Research Topics
| Sashi NadanacivaSummaryAffiliation: Pfizer Global Research and Development Country: USA Publications
| Collaborators
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Detail Information
Publications
High-content screening for compounds that affect mtDNA-encoded protein levels in eukaryotic cellsSashi Nadanaciva
Compound Safety Prediction, Worldwide Medicinal Chemistry, Pfizer Inc, Groton, CT 06340, USA
J Biomol Screen 15:937-48. 2010..62. The assay enables large-scale screening of compounds to identify those that potentially affect mtDNA-encoded protein levels and can be implemented within a screening paradigm to minimize compound attrition...- The role of mitochondrial dysfunction and drug safetySashi Nadanaciva
Pfizer Global R and D, Compound Safety Prediction, Worldwide Medicinal Chemistry, Eastern Point Road, Groton, CT 06340, USA
IDrugs 12:706-10. 2009..This feature review discusses the mechanisms by which drugs impair mitochondrial function, and describes some of the high-throughput methods being used to screen for drug-induced mitochondrial impairment... - Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesisSashi Nadanaciva
MitoSciences Inc, Eugene, OR 97403, USA
J Immunol Methods 343:1-12. 2009..The assay has high reproducibility, requires small amounts of sample, is quantitative, and is able to identify drugs which ultimately lead to a decrease in mtDNA-encoded proteins... - New insights in drug-induced mitochondrial toxicitySashi Nadanaciva
Compound Safety Prediction, Pfizer Global R and D, Eastern Point Rd, Groton, CT 06340, USA
Curr Pharm Des 17:2100-12. 2011.... - A high content screening assay for identifying lysosomotropic compoundsSashi Nadanaciva
Compound Safety Prediction, Worldwide Medicinal Chemistry, Pfizer Inc, Groton, CT 06340, USA
Toxicol In Vitro 25:715-23. 2011.... - Mitochondrial impairment by PPAR agonists and statins identified via immunocaptured OXPHOS complex activities and respirationSashi Nadanaciva
MitoSciences, Inc, 1850 Millrace Drive, Eugene, OR 97403, USA
Toxicol Appl Pharmacol 223:277-87. 2007..In several cases, the combination additively uncoupled or inhibited respiration, suggesting that some combinations are more likely to yield clinically relevant drug-induced mitochondrial side effects than others... - Current concepts in drug-induced mitochondrial toxicitySashi Nadanaciva
Pfizer Inc, Groton, Connecticut, USA
Curr Protoc Toxicol . 2009..This unit provides an overview on the physiological roles of mitochondria and the mechanisms by which they can be adversely affected by drugs... - Investigating mitochondrial dysfunction to increase drug safety in the pharmaceutical industrySashi Nadanaciva
Compound Safety Prediction, Pfizer Global R and D, Groton, CT 06340, USA
Curr Drug Targets 12:774-82. 2011..Potentially useful animal models and in vivo methods to detect drug-induced mitochondrial impairment are also discussed... - Effect of the multitargeted tyrosine kinase inhibitors imatinib, dasatinib, sunitinib, and sorafenib on mitochondrial function in isolated rat heart mitochondria and H9c2 cellsYvonne Will
Exploratory Safety Differentiation, Pfizer, Inc, Groton, Connecticut 06340, USA
Toxicol Sci 106:153-61. 2008..For the other three kinase inhibitors lacking direct mitochondrial effects, altered kinase and other signaling pathways, are a more reasonable explanation for potential toxicity... - High-throughput assays for assessing mitochondrial dysfunction caused by compounds that impair mtDNA-encoded protein levels in eukaryotic cellsSashi Nadanaciva
Compound Safety Prediction, Worldwide Medicinal Chemistry, Pfizer Inc, Groton, Connecticut, USA
Curr Protoc Toxicol . 2011..The third assay is an in-cell immunoassay that utilizes colorimetric detection of the two proteins and requires an absorbance microplate reader... - Mitochondrial membrane potential measurement of H9c2 cells grown in high-glucose and galactose-containing media does not provide additional predictivity towards mitochondrial assessmentPayal Rana
Compound Safety Prediction, Pfizer Global R and D, Groton, CT, USA
Toxicol In Vitro 25:580-7. 2011..We conclude that even though the multiplexed assay is useful for HTS applications, it provides no additional value over the high-glucose-galactose cell viability assay... - Analysis of protein fragmentation inhibition by an MMP-inhibitor in an in vivo model of heart failure using automated chromatographyJ Randall Slemmon
Genomics and Biotechnology, Pfizer Global Research and Development, Skokie, IL 60077, USA
J Chromatogr B Analyt Technol Biomed Life Sci 819:219-28. 2005..The system is flexible and amenable to improvements such as incorporating detection by mass spectrometry... - Biguanide-induced mitochondrial dysfunction yields increased lactate production and cytotoxicity of aerobically-poised HepG2 cells and human hepatocytes in vitroJames A Dykens
Drug Safety Research and Development, Pfizer, Inc, Ramsgate Rd Sandwich, CT13 9NJ, UK
Toxicol Appl Pharmacol 233:203-10. 2008..Indeed, the desired clinical outcome, viz., decreased blood glucose, could be due to increased glucose uptake and glycolytic flux in response to drug-induced mitochondrial dysfunction... - The contribution of oxidative stress to drug-induced organ toxicity and its detection in vitro and in vivoCláudia V Pereira
Pfizer R and D, Compound Safety Prediction WWMC, Cell Based Assays and Mitochondrial Biology, Eastern Point Rd, Groton, CT 06340, USA
Expert Opin Drug Metab Toxicol 8:219-37. 2012..Although new and more efficient assays and models are being developed, the hunt for biomarkers and noninvasive techniques is still in progress... - Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assaysSashi Nadanaciva
MitoSciences Inc, 1850 Millrace Drive, Eugene, OR 97403, USA
Toxicol In Vitro 21:902-11. 2007..A major advantage of the immunocapture approach is that it can be used throughout drug screening from early compound evaluation to clinical trials...