Research Topics
Species | Mark P MolloySummaryAffiliation: Pfizer Global Research and Development Country: USA Publications
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Detail Information
Publications
Overcoming technical variation and biological variation in quantitative proteomicsMark P Molloy
Pfizer Global Research and Development, Ann Arbor Laboratories, Ann Arbor, MI 48105, USA
Proteomics 3:1912-9. 2003....
Large-scale evaluation of quantitative reproducibility and proteome coverage using acid cleavable isotope coded affinity tag mass spectrometry for proteomic profilingMark P Molloy
Pfizer Global Research and Development, Department of Molecular Technologies, Michigan Laboratories, Ann Arbor, MI, USA
Proteomics 5:1204-8. 2005..However, the method was strongly biased to detect acidic proteins (pI < 7), under-represented small proteins (<10 kDa) and failed to show clear superiority over 2-DE methods in monitoring hydrophobic proteins from cell lysates...
Isolation of bacterial cell membranes proteins using carbonate extractionMark P Molloy
Australian Proteome Analysis Facility Ltd, Sydney, Australia
Methods Mol Biol 424:397-401. 2008..With the aid of strong sample solubilising reagents compatible with two-dimensional electrophoresis (2-DE), many of the carbonate-enriched membrane proteins can be separated using standard 2-DE techniques...
Polyomic profiling reveals significant hepatic metabolic alterations in glucagon-receptor (GCGR) knockout mice: implications on anti-glucagon therapies for diabetesJianxin Yang
Pfizer Global Research and Development, Groton, CT, USA
BMC Genomics 12:281. 2011..We sought to determine the hepatic & systemic consequence of full glucagon receptor antagonism through the study of the glucagon receptor knock-out mouse (Gcgr-/-) compared to wild-type littermates...
Proteomics: technologies and applicationsMark P Molloy
Pfizer Global Research and Development, Ann Arbor Laboratories, USA
Brief Funct Genomic Proteomic 1:23-39. 2002..Some areas where proteomics has delivered valuable biological insight will also be considered, and there will be a look towards future areas of development...
Proteomic analysis of mdx skeletal muscle: Great reduction of adenylate kinase 1 expression and enzymatic activityYue Ge
Graduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, 48109, USA
Proteomics 3:1895-903. 2003..Our results suggested that decreased AK 1 expression and activity might result in redistribution of energy flow through the alternative CK system, thus a compensatory potential might limit cellular energy failure in mdx skeletal muscle...
Profiling the alkaline membrane proteome of Caulobacter crescentus with two-dimensional electrophoresis and mass spectrometryMark P Molloy
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Proteomics 2:899-910. 2002..crescentus membrane proteome. Our experiments point towards new options for proteomic researchers aiming to both extend the scope of analysis, and simplify methods of identifying proteins with high confidence...
Exploring the proteome: reviving emphasis on quantitative protein profilingRuth A VanBogelen
Proteomics 3:1833-4. 2003
The challenge of industrializing proteomicsMark P Molloy
Nat Biotechnol 21:597. 2003
Proteomic identification of lynchpin urokinase plasminogen activator receptor protein interactions associated with epithelial cancer malignancyRohit G Saldanha
Australian Proteome Analysis Facility Ltd and Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, 2109, NSW Australia
J Proteome Res 6:1016-28. 2007..Our findings support the notion that uPAR behaves as a lynchpin in promoting tumorigenesis by forming functionally active multiprotein complexes...
Comparing SILAC and two-dimensional gel electrophoresis image analysis for profiling urokinase plasminogen activator signaling in ovarian cancer cellsPauliina M Uitto
Department of Chemistry and Biomolecular Sciences, Macquarie University, 2109, Sydney, NSW, Australia
J Proteome Res 6:2105-12. 2007..However, due to the lower variances associated with the SILAC technique, smaller changes in expression of uPA-inducible proteins could be found with greater certainty...
Differential expression of the skeletal muscle proteome in mdx mice at different agesYue Ge
Graduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor 48109-0606, USA
Electrophoresis 25:2576-85. 2004..Finally, we analyzed alterations of protein expression in mdx mice at one and six months of age to determine how protein expression changes with disease progression...
