JORGE CROSA

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. ncbi Genetics and assembly line enzymology of siderophore biosynthesis in bacteria
    Jorge H Crosa
    Department of Molecular Microbiology and Immunology, School of Medicine Oregon Health and Science University, Portland, Oregon 97201, USA
    Microbiol Mol Biol Rev 66:223-49. 2002
  2. ncbi Homodimerization and Binding of Specific Domains to the Target DNA Are Essential Requirements for HlyU To Regulate Expression of the Virulence Gene rtxA1, Encoding the Repeat-in-Toxin Protein in the Human Pathogen Vibrio vulnificus
    Moqing Liu
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239
    J Bacteriol 193:6895-901. 2011
  3. ncbi Signal transduction and transcriptional and posttranscriptional control of iron-regulated genes in bacteria
    J H Crosa
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA
    Microbiol Mol Biol Rev 61:319-36. 1997
  4. ncbi Molecular and genetic characterization of the TonB2-cluster TtpC protein in pathogenic vibrios
    Carole J Kuehl
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Biometals 22:109-15. 2009
  5. ncbi Iron transport in Francisella in the absence of a recognizable TonB protein still requires energy generated by the proton motive force
    Lidia M Crosa
    Department of Molecular Microbiology and Immunology, School of Medicine, Oregon Health and Science University, Portland, OR, USA
    Biometals 22:337-44. 2009
  6. ncbi A novel protein, TtpC, is a required component of the TonB2 complex for specific iron transport in the pathogens Vibrio anguillarum and Vibrio cholerae
    Michiel Stork
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239 3098, USA
    J Bacteriol 189:1803-15. 2007
  7. ncbi Reactivation of the vanchrobactin siderophore system of Vibrio anguillarum by removal of a chromosomal insertion sequence originated in plasmid pJM1 encoding the anguibactin siderophore system
    Hiroaki Naka
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Environ Microbiol 10:265-77. 2008
  8. ncbi Tandem heterocyclization domains in a nonribosomal peptide synthetase essential for siderophore biosynthesis in Vibrio anguillarum
    Manuela Di Lorenzo
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239 3098, USA
    Biometals 21:635-48. 2008
  9. ncbi Two tonB systems function in iron transport in Vibrio anguillarum, but only one is essential for virulence
    Michiel Stork
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, SW Sam Jackson Park Road, Portland, OR 97239, USA
    Infect Immun 72:7326-9. 2004
  10. ncbi Genetic and transcriptional analysis of the siderophore malleobactin biosynthesis and transport genes in the human pathogen Burkholderia pseudomallei K96243
    Alejandro F Alice
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Bacteriol 188:1551-66. 2006

Research Grants

  1. Assembly Line Biosynthesis of Bacterial Siderophores
    JORGE CROSA; Fiscal Year: 2005
  2. Iron Uptake and Virulence of Burkholderia pseudomallei
    JORGE CROSA; Fiscal Year: 2005
  3. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 2006
  4. Iron and pathogenesis in infections by Vibrio vulnificus
    JORGE CROSA; Fiscal Year: 2007
  5. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 2007
  6. Assembly Line Biosynthesis of Bacterial Siderophores
    JORGE CROSA; Fiscal Year: 2009
  7. Iron and pathogenesis in infections by Vibrio vulnificus
    Jorge H Crosa; Fiscal Year: 2010
  8. Assembly Line Biosynthesis of Bacterial Siderophores
    Jorge H Crosa; Fiscal Year: 2010
  9. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    Jorge H Crosa; Fiscal Year: 2010
  10. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 1993

Collaborators

  • Christopher Walsh
  • Carlos R Osorio
  • RANJAN N CHAKRABORTY
  • Manuel L Lemos
  • Michiel Stork
  • Hiroaki Naka
  • Claudia S López
  • Manuela Di Lorenzo
  • Alejandro F Alice
  • Moqing Liu
  • Timothy J Welch
  • Marcelo E Tolmasky
  • Lidia M Crosa
  • Caleb W Dorsey
  • Luis A Actis
  • Carole J Kuehl
  • Cristiane C Thompson
  • Christopher Dubay
  • Fabiano L Thompson
  • Graciela M Dias
  • Michael Rose
  • R Sean Peacock
  • Fred Heffron
  • Hans J Vogel
  • Ben R Otto
  • Maria A Ledesma
  • Carolyn A Lowe
  • Maho Nagasawa
  • Pamela L Connerly
  • Sophie Poppelaars
  • Andrew P Tomaras
  • David Farrell
  • Lillian Waldbeser
  • Patricia Salinas
  • Qian Chen
  • Anne M Wertheimer

Detail Information

Publications26

  1. ncbi Genetics and assembly line enzymology of siderophore biosynthesis in bacteria
    Jorge H Crosa
    Department of Molecular Microbiology and Immunology, School of Medicine Oregon Health and Science University, Portland, Oregon 97201, USA
    Microbiol Mol Biol Rev 66:223-49. 2002
    ....
  2. ncbi Homodimerization and Binding of Specific Domains to the Target DNA Are Essential Requirements for HlyU To Regulate Expression of the Virulence Gene rtxA1, Encoding the Repeat-in-Toxin Protein in the Human Pathogen Vibrio vulnificus
    Moqing Liu
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239
    J Bacteriol 193:6895-901. 2011
    ..We also determined that HlyU homodimer formation is an essential requirement for binding to the upstream region of the rtxA1 operon and is the key feature in relieving the H-NS repression of rtxA1 transcription...
  3. ncbi Signal transduction and transcriptional and posttranscriptional control of iron-regulated genes in bacteria
    J H Crosa
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA
    Microbiol Mol Biol Rev 61:319-36. 1997
    ..I hope that by using these diverse paradigms, I will be able to convey a unifying picture of these mechanism and their importance in the maintenance and prosperity of bacteria within their ecological niches...
  4. ncbi Molecular and genetic characterization of the TonB2-cluster TtpC protein in pathogenic vibrios
    Carole J Kuehl
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Biometals 22:109-15. 2009
    ....
  5. ncbi Iron transport in Francisella in the absence of a recognizable TonB protein still requires energy generated by the proton motive force
    Lidia M Crosa
    Department of Molecular Microbiology and Immunology, School of Medicine, Oregon Health and Science University, Portland, OR, USA
    Biometals 22:337-44. 2009
    ..Our studies suggest that alternative pathways to internalize iron might exist in Francisella...
  6. ncbi A novel protein, TtpC, is a required component of the TonB2 complex for specific iron transport in the pathogens Vibrio anguillarum and Vibrio cholerae
    Michiel Stork
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239 3098, USA
    J Bacteriol 189:1803-15. 2007
    ..This novel component of the TonB2 system found in V. anguillarum and V. cholerae is perhaps a general feature in bacteria harboring the Vibrio-like TonB2 system...
  7. ncbi Reactivation of the vanchrobactin siderophore system of Vibrio anguillarum by removal of a chromosomal insertion sequence originated in plasmid pJM1 encoding the anguibactin siderophore system
    Hiroaki Naka
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Environ Microbiol 10:265-77. 2008
    ..Our results underscore the importance of the anguibactin system in the survival of V. anguillarum 775 under conditions of iron limitation...
  8. ncbi Tandem heterocyclization domains in a nonribosomal peptide synthetase essential for siderophore biosynthesis in Vibrio anguillarum
    Manuela Di Lorenzo
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239 3098, USA
    Biometals 21:635-48. 2008
    ..Regulation of the angN gene follows the patterns observed at the iron transport-biosynthesis promoter with angN transcription repressed in the presence of iron and enhanced by AngR and trans-acting factor (TAF) under iron limitation...
  9. ncbi Two tonB systems function in iron transport in Vibrio anguillarum, but only one is essential for virulence
    Michiel Stork
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, SW Sam Jackson Park Road, Portland, OR 97239, USA
    Infect Immun 72:7326-9. 2004
    ..Each of the tonB genes is transcribed in an operon with the cognate exbB and exbD genes in response to iron limitation. Only tonB2 is essential for transport of ferric anguibactin and virulence...
  10. ncbi Genetic and transcriptional analysis of the siderophore malleobactin biosynthesis and transport genes in the human pathogen Burkholderia pseudomallei K96243
    Alejandro F Alice
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Bacteriol 188:1551-66. 2006
    ..Interestingly, the transcription of the mbaA, fmtA, and mbaS genes is not controlled by the availability of the siderophore malleobactin...
  11. ncbi Global gene expression as a function of the iron status of the bacterial cell: influence of differentially expressed genes in the virulence of the human pathogen Vibrio vulnificus
    Alejandro F Alice
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    Infect Immun 76:4019-37. 2008
    ....
  12. ncbi Novel role of the lipopolysaccharide O1 side chain in ferric siderophore transport and virulence of Vibrio anguillarum
    Timothy J Welch
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University; 3181 Sam Jackson Park Road, Portland, OR 97239, USA
    Infect Immun 73:5864-72. 2005
    ..anguillarum: serum resistance and anguibactin-mediated iron transport. These factors allow V. anguillarum to survive in serum and multiply in the iron-limiting milieu of the host vertebrate...
  13. ncbi Plasmid- and chromosome-encoded redundant and specific functions are involved in biosynthesis of the siderophore anguibactin in Vibrio anguillarum 775: a case of chance and necessity?
    Alejandro F Alice
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    J Bacteriol 187:2209-14. 2005
    ..We also identified in this cluster a chromosomal angA gene that is essential in anguibactin biosynthesis...
  14. ncbi Molecular characterization of the TonB2 protein from the fish pathogen Vibrio anguillarum
    Claudia S López
    Department of Molecular Microbiology and Immunology L 220, Oregon Health and Science University, Portland, OR 97239, USA
    Biochem J 418:49-59. 2009
    ..Overall our results indicate that TonB2 is a representative of a different class of TonB proteins...
  15. ncbi Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775
    Manuela Di Lorenzo
    Department of Molecular Microbiology and Immunology, Oregon HealthScience University, Portland, Oregon 97201, USA
    J Bacteriol 185:5822-30. 2003
    ..We also show that there is considerable microheterogeneity in pJM1-like plasmids from virulent strains of V. anguillarum isolated from different geographical sources...
  16. ncbi Characterization of ferric-anguibactin transport in Vibrio anguillarum
    Claudia S López
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Biometals 20:393-403. 2007
    ..Deletion of the plug domain resulted in a receptor unable to act as an open channel for the transport of the ferric anguibactin complex...
  17. ncbi A nonribosomal peptide synthetase with a novel domain organization is essential for siderophore biosynthesis in Vibrio anguillarum
    Manuela Di Lorenzo
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97201, USA
    J Bacteriol 186:7327-36. 2004
    ..Analysis of the sequence at this promoter showed that it overlaps the iron transport-biosynthesis promoter and operates in the opposite direction...
  18. ncbi Transcription termination within the iron transport-biosynthesis operon of Vibrio anguillarum requires an antisense RNA
    Michiel Stork
    Department of Molecular Microbiology and Immunology L 220, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201 3098, USA
    J Bacteriol 189:3479-88. 2007
    ..This termination process requires a 427-nucleotide antisense RNA that spans the intergenic region and acts as a novel transcriptional terminator...
  19. ncbi Identification of amino acid residues required for ferric-anguibactin transport in the outer-membrane receptor FatA of Vibrio anguillarum
    Claudia S López
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Microbiology 153:570-84. 2007
    ..It was also shown that the conserved arginine 428 located in strand 11 is essential for transport. The results suggest that a conformational change or partial unfolding of the plug domain occurs during ferric-anguibactin transport...
  20. ncbi Complete genome sequence of the marine fish pathogen Vibrio anguillarum harboring the pJM1 virulence plasmid and genomic comparison with other virulent strains of V. anguillarum and V. ordalii
    Hiroaki Naka
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    Infect Immun 79:2889-900. 2011
    ..ordalii. Mobile genetic elements, some of them possibly originated in the pJM1 plasmid, were very abundant in 775, resulting in the silencing of specific genes, with only few insertions in the 96F and RV22 chromosomes...
  21. ncbi HlyU acts as an H-NS antirepressor in the regulation of the RTX toxin gene essential for the virulence of the human pathogen Vibrio vulnificus CMCP6
    Moqing Liu
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Microbiol 72:491-505. 2009
    ..Competitive footprinting and gel shift data demonstrate HlyU's higher affinity as compared with H-NS resulting in the de-repression and a corresponding increased expression of the rtxA1 operon...
  22. ncbi The HlyU protein is a positive regulator of rtxA1, a gene responsible for cytotoxicity and virulence in the human pathogen Vibrio vulnificus
    Moqing Liu
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Infect Immun 75:3282-9. 2007
    ..Activation of the expression of the rtxA1 operon by HlyU occurs at the transcription initiation level by binding of the HlyU protein to a region upstream of this operon...
  23. ncbi Plasmid-mediated iron uptake and virulence in Vibrio anguillarum
    Michiel Stork
    Department of Molecular Microbiology and Immunology, School of Medicine L-220, Oregon Health and Science University, Portland, Oregon 97201, USA
    Plasmid 48:222-8. 2002
    ..We also have evidence that the siderophore itself plays a positive role in the regulatory mechanism of the expression of both transport and biosynthesis genes...
  24. ncbi The siderophore-mediated iron acquisition systems of Acinetobacter baumannii ATCC 19606 and Vibrio anguillarum 775 are structurally and functionally related
    Caleb W Dorsey
    Department of Microbiology, 40 Pearson Hall, Miami University, Oxford, OH 45056, USA
    Microbiology 150:3657-67. 2004
    ..Taken together, these results demonstrate that these pathogens, which cause serious infections in unrelated hosts, express very similar siderophore-mediated iron-acquisition systems...
  25. ncbi Genetic organization of an Acinetobacter baumannii chromosomal region harbouring genes related to siderophore biosynthesis and transport
    Caleb W Dorsey
    Department of Microbiology, Miami University, Oxford, OH 45056, USA
    Microbiology 149:1227-38. 2003
    ..This arrangement explains the iron- and Fur-regulated expression of om73, and provides strong evidence for a similar regulation for the expression of p114...

Research Grants39

  1. Assembly Line Biosynthesis of Bacterial Siderophores
    JORGE CROSA; Fiscal Year: 2005
    ....
  2. Iron Uptake and Virulence of Burkholderia pseudomallei
    JORGE CROSA; Fiscal Year: 2005
    ..Knowledge gained from our investigation will contribute to the development of measures to control the diseases caused by these pathogens. ..
  3. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 2006
    ..In the long term, our findings will aid in the development of measures for the control of septicemic diseases. ..
  4. Iron and pathogenesis in infections by Vibrio vulnificus
    JORGE CROSA; Fiscal Year: 2007
    ..These studies will lead to an enhanced understanding of the pathogenesis of V. vulnificus infections in particular and of bacterial virulence in general. ..
  5. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 2007
    ..vulnificus, and V. cholerae that encodes a novel protein essential for tonB2-mediated iron transport. We will characterize this protein, TtpC, to understand its role in iron transport and virulence of V. anguillarum and V. vulnificus. ..
  6. Assembly Line Biosynthesis of Bacterial Siderophores
    JORGE CROSA; Fiscal Year: 2009
    ..These approaches will likely lead to the exploration of new avenues in the process of siderophore biosynthesis and virulence in bacteria. ..
  7. Iron and pathogenesis in infections by Vibrio vulnificus
    Jorge H Crosa; Fiscal Year: 2010
    ..These studies will lead to an enhanced understanding of the pathogenesis of V. vulnificus infections in particular and of bacterial virulence in general. ..
  8. Assembly Line Biosynthesis of Bacterial Siderophores
    Jorge H Crosa; Fiscal Year: 2010
    ..These approaches will likely lead to the exploration of new avenues in the process of siderophore biosynthesis and virulence in bacteria. ..
  9. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    Jorge H Crosa; Fiscal Year: 2010
    ..vulnificus, and V. cholerae that encodes a novel protein essential for tonB2-mediated iron transport. We will characterize this protein, TtpC, to understand its role in iron transport and virulence of V. anguillarum and V. vulnificus. ..
  10. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 1993
    ..In summary, the molecular studies of this plasmid-mediated iron-uptake system should provide useful information in both the basic aspects of plasmid-mediated iron uptake and its role as a bacterial virulence factor...
  11. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 2000
    ..An understanding of signal transduction in bacteria may also reveal new strategies for combating infectious disease. Results obtained in this system are thus directly applicable to other systems involved in pathogenesis. ..
  12. Assembly Line Biosynthesis of Bacterial Siderophores
    JORGE CROSA; Fiscal Year: 2009
    ..These approaches will likely lead to the exploration of new avenues in the process of siderophore biosynthesis and virulence in bacteria. ..