Research Topics
Species | Robert A ColvinSummaryAffiliation: Ohio University Country: USA Publications
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Publications
pH dependence and compartmentalization of zinc transported across plasma membrane of rat cortical neuronsRobert A Colvin
Program in Neuroscience, Department of Biological Sciences, Ohio University, Athens, Ohio 45701, USA
Am J Physiol Cell Physiol 282:C317-29. 2002..4. However, rapid restoration of punctate zinquin labeling was not observed when cells were placed in 30 microM Zn(2+), pH 6.0. These data were confirmed in parallel (65)Zn(2+) transport experiments...- Insights into Zn2+ homeostasis in neurons from experimental and modeling studiesRobert A Colvin
Dept of Biological Sciences, Ohio Univ, Athens, OH 45701, USA
Am J Physiol Cell Physiol 294:C726-42. 2008..We conclude that maintenance of neuronal Zn2+ homeostasis, when challenged with Zn2+ loads, relies heavily on the function of a high-affinity muffler, the characteristics of which can be effectively studied with computational models... - Cytosolic zinc buffering and muffling: their role in intracellular zinc homeostasisRobert A Colvin
Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
Metallomics 2:306-17. 2010..We suggest that the biological functions of transient changes in cytosolic zinc ion concentrations (presumptive zinc signals) complement those of calcium ions in both spatial and temporal dimensions... - Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartmentRobert A Colvin
Department of Biological Sciences, Program in Neuroscience, Ohio University, Athens, OH 45701, USA
Brain Res 1085:1-10. 2006..We demonstrated real time trafficking of sequestered Zn(2+), using zinquin fluorescence, apparently associated with an endosome-like compartment or protein complexes in the cytosol... - Evidence for pH dependent Zn2+influx in K562 erythroleukemia cells: studies using ZnAF-2F fluorescence and 65Zn2+ uptakeRobert A Colvin
Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
Arch Biochem Biophys 442:222-8. 2005..34). The IC50 for proton effects was close to the pKa for histidine, suggesting conserved histidine residues present in SLC39A1 play a critical role in Zn2+ influx and are involved in the pH effect... - Zn2+ transporters and Zn2+ homeostasis in neuronsRobert A Colvin
Program in Neuroscience, Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
Eur J Pharmacol 479:171-85. 2003..Finally, this review presents a working model of neuronal Zn2+ homeostasis and discusses the experimental evidence for the proposed roles that Zn2+ transporters might play... - Mechanisms of Zn2+ efflux in cultured cortical neuronsYan Qin
Department of Biological Sciences, Ohio University, Athens, Ohio 45701, USA
J Neurochem 107:1304-13. 2008..6-8);whereas an effect of Ca2+ was not observed at pHo 8. In summary, a Na+, Ca2+/Zn2+ exchanger mechanism is proposed to be the primary transport mechanism that extrudes Zn2+ when neuronal intracellular free Zn2+ levels rise... - Silencing of ZnT1 reduces Zn2+ efflux in cultured cortical neuronsYan Qin
Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
Neurosci Lett 450:206-10. 2009.... - A proton-dependent zinc uptake in PC12 cellsRengarajan V Balaji
Program in Neuroscience, Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
Neurochem Res 30:171-6. 2005..000277 pH units per minute (assuming a 1:2 zinc:proton stoichiometry). This study adds a new dimension towards understanding the role played by intracellular pH in causing zinc entry into cells... - Release of intracellular Zn(2+) in cultured neurons after brief exposure to low concentrations of exogenous nitric oxideWei Lin
Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
Biometals 20:891-901. 2007..The possibility that release of intracellular Zn(2+) in response to NO could play a role in intracellular signaling is discussed...
Research Grants
- Zinc transport and homeostasis in cortical neuronsRobert Colvin; Fiscal Year: 2002..What is the identity of Zn2+ containing compartments in the cell body and processes of cortical neurons?..