Norma J Greenfield

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. ncbi Analysis of circular dichroism data
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    Methods Enzymol 383:282-317. 2004
  2. ncbi Solution NMR structure of the junction between tropomyosin molecules: implications for actin binding and regulation
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Mol Biol 364:80-96. 2006
  3. ncbi Using circular dichroism spectra to estimate protein secondary structure
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854 8021, USA
    Nat Protoc 1:2876-90. 2006
  4. ncbi The structure of the carboxyl terminus of striated alpha-tropomyosin in solution reveals an unusual parallel arrangement of interacting alpha-helices
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854 5635, USA
    Biochemistry 42:614-9. 2003
  5. ncbi Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Nat Protoc 1:2527-35. 2006
  6. ncbi Structure and tropomyosin binding properties of the N-terminal capping domain of tropomodulin 1
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Biophys J 88:372-83. 2005
  7. ncbi Structural requirements of tropomodulin for tropomyosin binding and actin filament capping
    Alla S Kostyukova
    Department of Neuroscience and Cell Biology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Biochemistry 44:4905-10. 2005
  8. ncbi Structure and interactions of the carboxyl terminus of striated muscle alpha-tropomyosin: it is important to be flexible
    Norma J Greenfield
    University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854 5635, USA
    Biophys J 83:2754-66. 2002
  9. ncbi Structure of the N terminus of a nonmuscle alpha-tropomyosin in complex with the C terminus: implications for actin binding
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Biochemistry 48:1272-83. 2009
  10. ncbi Molecular basis of tropomyosin binding to tropomodulin, an actin-capping protein
    Alla S Kostyukova
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 372:608-18. 2007

Collaborators

Detail Information

Publications20

  1. ncbi Analysis of circular dichroism data
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    Methods Enzymol 383:282-317. 2004
  2. ncbi Solution NMR structure of the junction between tropomyosin molecules: implications for actin binding and regulation
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Mol Biol 364:80-96. 2006
    ....
  3. ncbi Using circular dichroism spectra to estimate protein secondary structure
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854 8021, USA
    Nat Protoc 1:2876-90. 2006
    ..However, it does not give the residue-specific information that can be obtained by x-ray crystallography or NMR...
  4. ncbi The structure of the carboxyl terminus of striated alpha-tropomyosin in solution reveals an unusual parallel arrangement of interacting alpha-helices
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854 5635, USA
    Biochemistry 42:614-9. 2003
    ..We propose that unusual packing and flexibility are general features of coiled-coil domains in proteins that are involved in intermolecular interactions...
  5. ncbi Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Nat Protoc 1:2527-35. 2006
    ..Measurement of the spectra of five folded proteins and their unfolding curves at a single wavelength requires approximately 8 h...
  6. ncbi Structure and tropomyosin binding properties of the N-terminal capping domain of tropomodulin 1
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Biophys J 88:372-83. 2005
    ..Residues 55-62 and 76-92 display negative 1H-15N heteronuclear Overhauser enhancements showing they are flexible. The conformational dynamics of these residues may be important for actin capping activity...
  7. ncbi Structural requirements of tropomodulin for tropomyosin binding and actin filament capping
    Alla S Kostyukova
    Department of Neuroscience and Cell Biology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Biochemistry 44:4905-10. 2005
    ..The results suggest that molecular flexibility and binding to actin via an amphipathic helix are both required for tropomyosin-dependent capping of the pointed end of the actin filament...
  8. ncbi Structure and interactions of the carboxyl terminus of striated muscle alpha-tropomyosin: it is important to be flexible
    Norma J Greenfield
    University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854 5635, USA
    Biophys J 83:2754-66. 2002
    ....
  9. ncbi Structure of the N terminus of a nonmuscle alpha-tropomyosin in complex with the C terminus: implications for actin binding
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Biochemistry 48:1272-83. 2009
    ..We discuss the relevance of a stable but dynamic intermolecular junction for high-affinity binding to actin...
  10. ncbi Molecular basis of tropomyosin binding to tropomodulin, an actin-capping protein
    Alla S Kostyukova
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 372:608-18. 2007
    ....
  11. ncbi Tropomyosin ends determine the stability and functionality of overlap and troponin T complexes
    Thomas Palm
    Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    Biophys J 84:3181-9. 2003
    ..The circular dichroism results were corroborated by native gel electrophoresis, and the ability of the troponin T fragment to promote binding of full-length tropomyosins to filamentous actin...
  12. ncbi Functions of tropomyosin's periodic repeats
    Sarah E Hitchcock-DeGregori
    Department of Neuroscience and Cell Biology, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Biochemistry 41:15036-44. 2002
    ..We propose that the local structure and stability, and consequent flexibility, of the coiled coil are major determinants of actin affinity...
  13. ncbi Tropomyosin regulates elongation by formin at the fast-growing end of the actin filament
    Barbara Wawro
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Biochemistry 46:8146-55. 2007
    ..This role, in addition to its cooperative control of myosin function, establishes tropomyosin as a universal regulator of the multifaceted actin cytoskeleton...
  14. ncbi Circular dichroism analysis for protein-protein interactions
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, UMDNJ-Robert Johnson Medical School, Piscataway, NJ, USA
    Methods Mol Biol 261:55-78. 2004
    ..Changes in the stability of the protein complexes as a function of temperature or added denaturants, compared to the isolated proteins, can also be used to determine binding constants...
  15. ncbi Analysis of the kinetics of folding of proteins and peptides using circular dichroism
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Nat Protoc 1:2891-9. 2006
    ....
  16. ncbi Tropomyosin requires an intact N-terminal coiled coil to interact with tropomodulin
    Norma J Greenfield
    University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Biophys J 82:2580-91. 2002
    ....
  17. ncbi Tropomyosin: regulator of actin filaments
    Sarah E Hitchcock-DeGregori
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Adv Exp Med Biol 592:87-97. 2007
  18. ncbi Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854-8021, USA
    Nat Protoc 1:2733-41. 2006
    ..The experiments require 2-5 h...
  19. ncbi Effects of estradiol and 4-hydroxytamoxifen on the conformation, thermal stability, and DNA recognition of estrogen receptor beta
    Veena Vijayanathan
    Department of Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 125 Paterson Street, NB, NJ 08903, USA
    Biochem Cell Biol 85:1-10. 2007
    ..The increased thermal stability of HT-ERbeta-ERE was associated with reduced specificity of ERbeta-ERE recognition, illustrating profound differences in conformational states of ERbeta induced by E2 and HT...
  20. ncbi Tropomodulin contains two actin filament pointed end-capping domains
    Velia M Fowler
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 278:40000-9. 2003
    ..We conclude that the carboxyl-terminal capping domain of Tmod1 contains a TM-independent actin pointed end-capping activity, whereas the amino-terminal domain contains a TM-regulated pointed end actin-capping activity...