Research Topics
Species | David FrickSummaryAffiliation: New York Medical College Country: USA Publications
Research Grants
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Detail Information
Publications
Hepatitis C virus NS3 ATPases/helicases from different genotypes exhibit variations in enzymatic propertiesAngela M I Lam
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
J Virol 77:3950-61. 2003..NS3 residue 450 is a threonine in Hel-1a and Hel-1b and is an isoleucine in Hel-2a. A mutant Hel-1a with an isoleucine substituted for threonine 450 unwinds DNA more rapidly and binds DNA more tightly than the parent protein...- Step-by-step progress toward understanding the hepatitis C virus RNA helicaseDavid N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA
Hepatology 43:1392-5. 2006..The assay developed here should be useful in investigating a broad range of nucleic acid translocation motors... - The hepatitis C virus NS3 protein: a model RNA helicase and potential drug targetDavid N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Curr Issues Mol Biol 9:1-20. 2007.... - DNA primasesD N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
Annu Rev Biochem 70:39-80. 2001..The small eukaryotic primase subunit contains the active site for RNA synthesis, and its activity correlates with DNA replication during the cell cycle... - Role of divalent metal cations in ATP hydrolysis catalyzed by the hepatitis C virus NS3 helicase: magnesium provides a bridge for ATP to fuel unwindingDavid N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
J Mol Biol 365:1017-32. 2007..Binding of either free magnesium or free ATP to HCV helicase competes with MgATP, the true fuel for helicase movements, and leads to slower hydrolysis and nucleic acid unwinding... - Helicases as antiviral drug targetsDavid N Frick
Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Drug News Perspect 16:355-62. 2003..These new developments should eventually lead to new antiviral medications... - The nonstructural protein 3 protease/helicase requires an intact protease domain to unwind duplex RNA efficientlyDavid N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
J Biol Chem 279:1269-80. 2004..The data suggest that hepatitis C virus RNA helicase, and therefore viral replication, could be influenced by the rotations of the protease domain which likely occur during polyprotein processing... - Understanding helicases as a means of virus controlD N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Curr Pharm Des 12:1315-38. 2006.... - Electrostatic analysis of the hepatitis C virus NS3 helicase reveals both active and allosteric site locationsDavid N Frick
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Nucleic Acids Res 32:5519-28. 2004..When DNA was present in the structure, the calculated pK(a)s shifted for both Lys210 and Asp290, demonstrating how DNA binding might lead to electrostatic changes that stimulate ATP hydrolysis... - Monitoring helicase activity with molecular beaconsCraig A Belon
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Biotechniques 45:433-40, 442. 2008..The HCV genome-based molecular beacon helicase assay is used to demonstrate how such an assay can be used in high-throughput screens and to analyze HCV helicase inhibitors... - Enhanced nucleic acid binding to ATP-bound hepatitis C virus NS3 helicase at low pH activates RNA unwindingAngela M I Lam
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Nucleic Acids Res 32:4060-70. 2004.... - Two novel conserved motifs in the hepatitis C virus NS3 protein critical for helicase actionAngela M I Lam
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
J Biol Chem 278:44514-24. 2003..A model is presented explaining the roles of the Arg-clamp and the Phe-loop in the unwinding reaction... - Analysis of the evolutionary forces in an immunodominant CD8 epitope in hepatitis C virus at a population levelChristoph Neumann-Haefelin
Department of Medicine II, University of Freiburg, Hugstetter Str 55, 79106 Freiburg, Germany
J Virol 82:3438-51. 2008.... - Effects of mutagenic and chain-terminating nucleotide analogs on enzymes isolated from hepatitis C virus strains of various genotypesJulie A Heck
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
Antimicrob Agents Chemother 52:1901-11. 2008..These results suggest that NS3 can limit the incorporation of ribavirin into viral RNA, thus reducing its inhibitory or mutagenic effects... - Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicaseAngela M I Lam
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
J Virol 80:404-11. 2006..These results demonstrate that the RNA-unwinding activity of the HCV NS3 helicase is needed for RNA replication...
Research Grants
- Enzymatic Differences Among Hepatitis C Virus GenotypesDavid Frick; Fiscal Year: 2003....
- Enzymatic Differences Among Hepatitis C Virus GenotypesDavid Frick; Fiscal Year: 2006..abstract_text> ..
- Enzymatic Differences Among Hepatitis C Virus GenotypesDavid Frick; Fiscal Year: 2007..abstract_text> ..