Research Topics
| James B DelehantySummaryAffiliation: Naval Medical Center Country: USA Publications
| Collaborators
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Detail Information
Publications
Delivering quantum dot-peptide bioconjugates to the cellular cytosol: escaping from the endolysosomal systemJames B Delehanty
Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
Integr Biol (Camb) 2:265-77. 2010..Importantly, this QD-peptide bioconjugate elicited minimal cytotoxicity in the cell lines tested...- Spatiotemporal multicolor labeling of individual cells using peptide-functionalized quantum dots and mixed delivery techniquesJames B Delehanty
Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
J Am Chem Soc 133:10482-9. 2011..Using this overall approach, QDs were targeted to both early and late endosomes, the cellular cytosol, and the plasma membrane in live cells, ultimately allowing for simultaneous five-color fluorescent imaging... - Binding and neutralization of lipopolysaccharides by plant proanthocyanidinsJames B Delehanty
Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
J Nat Prod 70:1718-24. 2007..Our results identify PACs as a new class of LPS-binding compound and suggest that they have potential utility in applications that necessitate either the purification and removal of LPS or the in vivo neutralization of LPS... - Multifunctional compact zwitterionic ligands for preparing robust biocompatible semiconductor quantum dots and gold nanoparticlesKimihiro Susumu
Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
J Am Chem Soc 133:9480-96. 2011..The strong potential of these ligands to expand NP capabilities in many biological applications is highlighted... - Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensingIgor L Medintz
Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
Nat Mater 9:676-84. 2010..A detailed mechanism describing the QD quenching processes that is consistent with dopamine's inherent redox chemistry is presented... - Cellular uptake and fate of PEGylated gold nanoparticles is dependent on both cell-penetration peptides and particle sizeEunkeu Oh
Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, DC 20375, USA
ACS Nano 5:6434-48. 2011..The 16 nm and larger AuNPs did not enter the cells and were located at the cellular periphery. A preliminary assessment of cytotoxicity demonstrated minimal effects on cellular viability following peptide-mediated uptake... - Intracellular bioconjugation of targeted proteins with semiconductor quantum dotsKelly Boeneman
Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Avenue, S W, Washington, DC 20375, USA
J Am Chem Soc 132:5975-7. 2010..This approach can facilitate long-term monitoring of their spatio-temporal activity or, alternatively, allow engineering and in situ assembly of designer chimeric QD-fluorescent protein sensors... - Intracellular delivery of quantum dot-protein cargos mediated by cell penetrating peptidesIgor L Medintz
Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
Bioconjug Chem 19:1785-95. 2008..Use of QD-peptide/fluorescent protein vectors may make powerful tools for understanding the mechanisms of nanoparticle-mediated drug delivery... - Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dotsDuane E Prasuhn
Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
ACS Nano 4:267-78. 2010..This modular approach for displaying peptides or DNA on QDs may be extended to other more complex biomolecules such as proteins or utilized with different types of nanoparticle materials... - Multidentate poly(ethylene glycol) ligands provide colloidal stability to semiconductor and metallic nanocrystals in extreme conditionsMichael H Stewart
Optical Sciences Division, Naval Research Laboratory, Washington, D C 20375, USA
J Am Chem Soc 132:9804-13. 2010..The improved colloidal stability of nanocrystals afforded by the tetradentate ligands was further demonstrated via the assembly of stable QD-nuclear localization signal peptide bioconjugates that promoted intracellular uptake... - Self-assembled quantum dot-peptide bioconjugates for selective intracellular deliveryJames B Delehanty
Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, USA
Bioconjug Chem 17:920-7. 2006..The efficacy of using peptides for selective intracellular delivery is highlighted by performing a multicolor QD labeling, where we found that the presence or absence of peptide on the QD surface controls cellular uptake... - Quantum dots: a powerful tool for understanding the intricacies of nanoparticle-mediated drug deliveryJames B Delehanty
Center for Bio Molecular Science and Engineering, SW Washington, DC 20375, USA
Expert Opin Drug Deliv 6:1091-112. 2009..Important related issues such as QD biofunctionalization and toxicity are also discussed. The paper concludes with a perspective of how this field can be expected to develop in the future... - Enhancing the stability and biological functionalities of quantum dots via compact multifunctional ligandsKimihiro Susumu
Division of Optical Sciences and Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, D C 20375, USA
J Am Chem Soc 129:13987-96. 2007..The new functional surface ligands described here provide not only stable and highly water-soluble QDs but also simple and easy access to various biological entities... - Transfected cell microarrays for the expression of membrane-displayed single-chain antibodiesBaochuan Lin
United States Naval Research Laboratory, Center for Bio Molecular Science and Engineering, Washington, DC, USA
Methods Mol Biol 706:119-37. 2011..This approach provides for the high-throughput functional analysis of many different proteins in parallel and can be considerably more informative and cost-effective relative to more traditional protein expression techniques... - Quantum dots and fluorescent protein FRET-based biosensorsKelly Boeneman
Center for Biomolecular Science and Engineering, Washington, DC 20375, USA
Adv Exp Med Biol 733:63-74. 2012..We further show that QDs and fluorescent proteins can be conjugated together intracellularly with strong potential for live-cell imaging and biosensing applications... - Immobilized proanthocyanidins for the capture of bacterial lipopolysaccharidesBrandy J Johnson
Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, D C 20375 5348, USA
Anal Chem 80:2113-7. 2008..Molar comparison of polymyxin B to proanthocyanidins indicated that the Sepharose immobilized PACs have a binding affinity for LPS similar to that of polymyxin B... - Delivering quantum dots into cells: strategies, progress and remaining issuesJames B Delehanty
Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC, USA
Anal Bioanal Chem 393:1091-105. 2009..Finally, we conclude by providing a perspective of how this field can be expected to develop in the future... - Selection of cholera toxin specific IgNAR single-domain antibodies from a naïve shark libraryJinny L Liu
Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC 20375, USA
Mol Immunol 44:1775-83. 2007..The successful isolation of target specific sdAbs from one of our non-biased NAR libraries, demonstrate their ability to provide binders against an unacquainted antigen of interest... - A comparison of microscope slide substrates for use in transfected cell microarraysJames B Delehanty
Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375 5348, USA
Biosens Bioelectron 20:773-9. 2004..976). The transfection efficiency of HEK 293T/17 cells varied in accord with the mean spot diameter, demonstrating that the uptake of DNA was a function of the local DNA concentration on each substrate... - Transfected cell microarrays for the expression of membrane-displayed single-chain antibodiesJames B Delehanty
Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, D C 20375 5348, USA
Anal Chem 76:7323-8. 2004..More generally, our work details the utility of transfected cell microarrays in mediating the functional characterization of expressed membrane receptor proteins... - Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama libraryEllen R Goldman
Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC 20375, USA
Anal Chem 78:8245-55. 2006..The ability to rapidly select such rugged antibodies will enhance the reliability of immunoassays by extending shelf life and the capacity to function in hostile environments... - Semiconductor quantum dots in bioanalysis: crossing the valley of deathW Russ Algar
Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Avenue, South West, Washington, DC 20375, USA
Anal Chem 83:8826-37. 2011..We describe the burgeoning role of QDs in many different fields of bioanalyses and highlight the advantages afforded by their unique physical and optical properties... - Antiviral properties of cobalt(III)-complexesJames B Delehanty
Center for Bio Molecular Science and Engineering, 4555 Overlook Avenue SW, Washington, DC 20375, USA
Bioorg Med Chem 16:830-7. 2008..We discuss our findings within the context of the ability to further functionalize Co(III)hexammine to render it a highly specific antiviral therapeutic reagent... - Printing functional protein microarrays using piezoelectric capillariesJames B Delehanty
Center for Biomolecular Sciences and Engineering, Naval Research Laboratory, Washington, DC, USA
Methods Mol Biol 264:135-43. 2004..Further, this method results in the deposition of capture antibodies that adopt a more uniform spot morphology... - Elaborate nanoparticle-based traps for catching cytosolic players in the actJames B Delehanty
Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Code 6900, Washington DC 20375 USA
Chembiochem 13:30-3. 2012..The system uses the rapid, innate self-assembly of ferritin, expressing bait and prey molecules, into ordered nanoclusters whose morphology is tracked by using fluorescence microscopy... - Identification of important residues in metal-chelate recognition by monoclonal antibodiesJames B Delehanty
Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, USA
Biochemistry 42:14173-83. 2003.... - Method for printing functional protein microarraysJames B Delehanty
Naval Res Lab, Washington, DC
Biotechniques 34:380-5. 2003..The implications of these results on the future development of protein biochips are discussed... - A microarray immunoassay for simultaneous detection of proteins and bacteriaJames B Delehanty
Naval Res Lab, Washington, DC
Anal Chem 74:5681-7. 2002..2 x 10(4) cfu/mL, respectively. The assays presented here are very fast, as compared to previously published methods for measuring antibody-antigen interactions using microarrays (minutes versus hours)... - Monoclonal antibodies that recognize minimal differences in the three-dimensional structures of metal-chelate complexesR Mark Jones
Department of Ophthalmology, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
Bioconjug Chem 13:408-15. 2002..Antibody affinity correlated well with the extent of total structural difference for these metal-EDTA complexes...