Research Topics
| D J CarucciSummaryAffiliation: Naval Medical Center Country: USA Publications
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Detail Information
Publications
Exploring the proteome of PlasmodiumDaniel J Carucci
Malaria Program IDD, Naval Medical Research Center, Silver Spring, MD 20910, USA
Int J Parasitol 32:1539-42. 2002....- Malaria research in the post-genomic eraD J Carucci
Malaria Program, Naval Medical Research Center, 20910 7500, Silver Spring, MD 20910 7500, USA
Parasitol Today 16:434-8. 2000..These complete genomic sequence data are certain to provide the foundation for nearly all malaria research in the next decades, as discussed here by Dan Carucci... - Technologies for the study of gene and protein expression in PlasmodiumDaniel J Carucci
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
Philos Trans R Soc Lond B Biol Sci 357:13-6. 2002..This paper will address the use of these technologies as well as some of the computational tools that will be ultimately required to adequately study gene and protein expression in Plasmodium... - Genomic tools for gene and protein discovery in malaria: toward new vaccinesD J Carucci
Malaria Program, Naval Medical Research Center, 20910, Silver Spring, MD, USA
Vaccine 19:2315-8. 2001.... - Utilization of genomic sequence information to develop malaria vaccinesD L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
J Exp Biol 206:3789-802. 2003..Here, we present our strategy to exploit the genomic sequence of P. falciparum for malaria vaccine development... - Plasmodium yoelii: cloning and characterization of the gene encoding for the mitochondrial heat shock protein 60G I Sanchez
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland, 21205, USA
Exp Parasitol 93:181-90. 1999..falciparum blood stages. These results show that PyHsp60 and PfHsp60 genes are homologous and that of the PyHsp60 gene encodes a heat-inducible, intracellular protein that is expressed in several of the developmental stages of P. yoelii... - Induction of CD4(+) T cell-dependent CD8(+) type 1 responses in humans by a malaria DNA vaccineR Wang
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
Proc Natl Acad Sci U S A 98:10817-22. 2001..Demonstration in humans of elicitation of the class I restricted IFN-gamma responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine... - Immunogenicity and protective efficacy of a Plasmodium yoelii Hsp60 DNA vaccine in BALB/c miceG I Sanchez
Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
Infect Immun 69:3897-905. 2001..The observation that in some of the PyHsp60 DNA vaccine-immunized mice there was protection against infection or a delay in the onset of parasitemia after sporozoite challenge deserves further evaluation... - Determining liver stage parasite burden by real time quantitative PCR as a method for evaluating pre-erythrocytic malaria vaccine efficacyA A Witney
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Room 3A4O, Silver Spring, MD 20910-7500, USA
Mol Biochem Parasitol 118:233-45. 2001..Our data establish this quantitative real-time PCR assay to be a fast and reproducible way of accurately assessing liver stage parasite burden and vaccine efficacy in rodent malaria models... - Reduced immunogenicity of DNA vaccine plasmids in mixturesM Sedegah
Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
Gene Ther 11:448-56. 2004..Interactions between components in a multiplasmid DNA vaccine may limit the ability to use plasmid pools alone to induce responses against multiple targets simultaneously... - Functional genomic technologies applied to the control of the human malaria parasite, Plasmodium falciparumD J Carucci
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
Pharmacogenomics 2:137-42. 2001.... - The malaria genome sequencing project: complete sequence of Plasmodium falciparum chromosome 2M J Gardner
Institute of Genomic Research, Naval Medical Research Center, Rockville, MD, USA
Parassitologia 41:69-75. 1999..Within 2-3 years, the sequence of almost all P. falciparum genes will have been determined, paving the way for genetic, biochemical, and immunological research aimed at developing new drugs and vaccines against malaria... - The infectivity of Plasmodium yoelii in different strains of miceM Belmonte
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, Maryland 20910-7500, USA
J Parasitol 89:602-3. 2003..9 and 10.6 sporozoites. The ID50 obtained for CD-1 mice (147 sporozoites) was significantly higher... - Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSFM Sedegah
1Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
Genes Immun 5:553-61. 2004.... - Proteome analysis of rhoptry-enriched fractions isolated from Plasmodium merozoitesTobili Y Sam-Yellowe
Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115, USA
J Proteome Res 3:995-1001. 2004..Molecular characterization and understanding of the supramolecular organization of these novel potential rhoptry proteins may assist in the identification of new intervention targets for the asexual blood stages of malaria... - High-throughput generation of P. falciparum functional molecules by recombinational cloningJoão Carlos Aguiar
Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
Genome Res 14:2076-82. 2004..falciparum genome and will provide for a resource for the malaria resource community distributed through public repositories... - A small peptide (CEL-1000) derived from the beta-chain of the human major histocompatibility complex class II molecule induces complete protection against malaria in an antigen-independent mannerYupin Charoenvit
CEL-SCI Corporation, 8229 Boone Blvd, Suite 802, Vienna, VA 22182, USA
Antimicrob Agents Chemother 48:2455-63. 2004.... - Molecular approaches to malariaKlaus Lingelbach
FB Biology, Philipps University, Marburg, Germany
Mol Microbiol 54:575-87. 2004..In this report we summarize the results and new research avenues presented at a recent meeting held with the aim of developing interdisciplinary approaches to combat this disease... - Global analysis of transcript and protein levels across the Plasmodium falciparum life cycleKarine G Le Roch
Department of Cell Biology ICND202, The Scripps Research Institute, La Jolla, California 92037, USA
Genome Res 14:2308-18. 2004..Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation... - Proteomics approach reveals novel proteins on the surface of malaria-infected erythrocytesLaurence Florens
Department of Cell Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
Mol Biochem Parasitol 135:1-11. 2004..making them good targets for interventions with a broad specificity to various P. falciparum isolates... - Plasmodium post-genomics: an updateDaniel J Carucci
Grand Challenges in Global Health Initiative, Foundation for the National Institutes of Health, 45 Center Drive 3AN 44, Bethesda, MD 20892 460, USA
Trends Parasitol 20:558-61. 2004..This review attempts to capture some of the current efforts in the post-genomics era of malaria research and highlights the approaches discussed at the Molecular Approaches to Malaria 2004 meeting... - Functional characterization of an LCCL-lectin domain containing protein family in Plasmodium bergheiHolly E Trueman
Immunology and Infection Section, Department of Biological Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK
J Parasitol 90:1062-71. 2004..The mutant is not rescued by coparasitization of mosquitoes with a mixture Pblap1+ and Pblap1- homokaryotic oocysts... - Transcriptionally active PCR for antigen identification and vaccine development: in vitro genome-wide screening and in vivo immunogenicityDavid P Regis
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, United States
Mol Biochem Parasitol 158:32-45. 2008..These data support the potential of a TAP approach for rapid high throughput functional screening and identification of potential candidate vaccine antigens from genomic sequence data... - Induction of multi-antigen multi-stage immune responses against Plasmodium falciparum in rhesus monkeys, in the absence of antigen interference, with heterologous DNA prime/poxvirus boost immunizationGeorge Jiang
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
Malar J 6:135. 2007.... - Extended immunization intervals enhance the immunogenicity and protective efficacy of plasmid DNA vaccinesGary T Brice
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
Microbes Infect 9:1439-46. 2007..Our studies establish that the dosing interval significantly impacts the immunogenicity and protective efficacy of plasmid DNA vaccines... - Vaxfectin enhances immunogenicity and protective efficacy of P. yoelii circumsporozoite DNA vaccinesMartha Sedegah
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
Vaccine 24:1921-7. 2006..Clinical trials of pDNA vaccines have often used low pDNA doses based on a per kilogram weight basis. Formulation of pDNA vaccines in Vaxfectin may improve their potency in human clinical trials... - Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparumPaul R Sanders
The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050 Australia
J Biol Chem 280:40169-76. 2005..This insight into merozoite surfaces provides new opportunities for understanding both erythrocyte invasion and anti-parasite immunity... - Advances in malaria genomics since MIM Arusha, 2002Daniel J Carucci
Grand Challenges in Global Health, Foundation for the National Institutes of Health, 45 Center Drive, Bethesda, MD 20814, USA
Acta Trop 95:260-4. 2005.... - The Plasmodium falciparum sexual development transcriptome: a microarray analysis using ontology-based pattern identificationJason A Young
Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Mol Biochem Parasitol 143:67-79. 2005..gnf.org/publications/Gametocyte), shed light on the components of molecular mechanisms underlying parasite sexual development and other areas of malarial parasite biology... - Transcriptional analysis of in vivo Plasmodium yoelii liver stage gene expressionJohn B Sacci
Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
Mol Biochem Parasitol 142:177-83. 2005..This is the first comprehensive analysis of in vivo gene expression undertaken for the liver stage of P. yoelii, and provides insights into the differential expression of P. yoelii genes during this critical stage of development... - A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analysesNeil Hall
Pathogen Sequencing Unit, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Science 307:82-6. 2005..We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process... - A Plasmodium gene family encoding Maurer's cleft membrane proteins: structural properties and expression profilingTobili Y Sam-Yellowe
Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115, USA
Genome Res 14:1052-9. 2004..falciparum and Plasmodium yoelii yoelii, where no orthologs were predicted uniquely based on sequence similarity. These basic proteins with two transmembrane domains belong to a larger superfamily, which includes STEVORs and RIFINs... - Rodent models of malaria in the genomics eraJane M Carlton
Parasite Genomics Group, The Institute for Genomic Research, Rockville20850, USA
Trends Parasitol 18:100-2. 2002..The Rodent Malaria Genomics Symposium: Current Status and Future Directions was held on 15-16 November 2001 in Atlanta, GA, USA... - A multilateral effort to develop DNA vaccines against falciparum malariaSanjai Kumar
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
Trends Parasitol 18:129-35. 2002.... - Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCRPeter L Blair
Department of Biological Sciences, PO Box 369, University of Notre Dame, Notre Dame, IN 46556-0369, USA
Nucleic Acids Res 30:2224-31. 2002..Our analysis demonstrates that using real-time RT-PCR fluorogenic 5' nuclease assays is a sensitive, quantitative method for analysis of Plasmodium transcripts... - Persistence of protective immunity to malaria induced by DNA priming and poxvirus boosting: characterization of effector and memory CD8(+)-T-cell populationsMartha Sedegah
Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
Infect Immun 70:3493-9. 2002..These results demonstrate that a heterologous priming and boosting vaccination can provide lasting protection against malaria in this model system... - Quantitation of liver-stage parasites by automated TaqMan real-time PCRAdam A Witney
Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
Methods Mol Med 72:137-9. 2002 - Genome sequence of the human malaria parasite Plasmodium falciparumMalcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:498-511. 2002..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria... - Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoeliiJane M Carlton
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:512-9. 2002..This is the first genome sequence of a model eukaryotic parasite, and it provides insight into the use of such systems in the modelling of Plasmodium biology and disease... - A proteomic view of the Plasmodium falciparum life cycleLaurence Florens
Department of Cell Biology, The Scripps Research Institute, SR-11, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 419:520-6. 2002..The detection of chromosomal clusters encoding co-expressed proteins suggested a potential mechanism for controlling gene expression... - Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14Malcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:531-4. 2002..We report here the nucleotide sequences of chromosomes 10, 11 and 14, and a re-analysis of the chromosome 2 sequence. These chromosomes represent about 35% of the 23-megabase P. falciparum genome... - Proteomics in malariaJeffrey R Johnson
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Proteome Res 3:296-306. 2004..The high-throughput nature of these techniques is in accord with the pace of drug and vaccine development that have the potential to directly reduce the morbidity and mortality of disease... - PfSPATR, a Plasmodium falciparum protein containing an altered thrombospondin type I repeat domain is expressed at several stages of the parasite life cycle and is the target of inhibitory antibodiesRana Chattopadhyay
Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
J Biol Chem 278:25977-81. 2003..falciparum sporozoites specifically recognized the expression of this protein on transfected COS-7 cells. These data support the evaluation of this protein as a vaccine candidate... - Induction in humans of CD8+ and CD4+ T cell and antibody responses by sequential immunization with malaria DNA and recombinant proteinRuobing Wang
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
J Immunol 172:5561-9. 2004..The induction in humans of the three primary Ag-specific adaptive immune responses establishes a strategy for developing immunization regimens against diseases in desperate need of vaccines... - Safety, tolerability, and antibody responses in humans after sequential immunization with a PfCSP DNA vaccine followed by the recombinant protein vaccine RTS,S/AS02AJudith E Epstein
Malaria Program, Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
Vaccine 22:1592-603. 2004.... - Novel antigen identification method for discovery of protective malaria antigens by rapid testing of DNA vaccines encoding exons from the parasite genomeDiana Haddad
Naval Medical Research Center, Walter Reed Army Institute of Research, Silver Spring, MD, USA
Infect Immun 72:1594-602. 2004..We conclude that high-throughput cloning of exons into DNA vaccines and their screening is feasible and can rapidly identify new malaria vaccine candidate antigens... - Successful induction of CD8 T cell-dependent protection against malaria by sequential immunization with DNA and recombinant poxvirus of neonatal mice born to immune mothersMartha Sedegah
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
J Immunol 171:3148-53. 2003..yoelii parasites or immunized with the vaccine were protected and had excellent T cell responses. These data support assessment of this immunization strategy in neonates/young infants in areas in which malaria exacts its greatest toll... - Discovery of gene function by expression profiling of the malaria parasite life cycleKarine G Le Roch
Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 301:1503-8. 2003..Genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes... - Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic dataDenise L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA
Proc Natl Acad Sci U S A 100:9952-7. 2003..The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens... - A glycosylphosphatidylinositol anchor signal sequence enhances the immunogenicity of a DNA vaccine encoding Plasmodium falciparum sexual-stage antigen, Pfs230Sarah L Fanning
Department of Biology, Loyola University Chicago, 6525 North Sheridan Road, Chicago, IL, 60626, USA
Vaccine 21:3228-35. 2003..p.) injection of E. coli-produced recombinant Pfs230 region C. None of these vaccination strategies were more effective than r230/MBP.C alone in generating malaria transmission-blocking immunity... - Purification of chromosomes from Plasmodium falciparumDaniel J Carucci
Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
Methods Mol Med 72:235-40. 2002 - Optimal induction of antigen-specific CD8+ T cell responses requires bystander cell participationGary T Brice
Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
J Leukoc Biol 72:1164-71. 2002..Our data further establish a novel role for NK cells in the activation of antigen-specific immune responses...