Keiko Ozato

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi Brd4 is required for recovery from antimicrotubule drug-induced mitotic arrest: preservation of acetylated chromatin
    Akira Nishiyama
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Mol Biol Cell 17:814-23. 2006
  2. ncbi IRF family proteins and type I interferon induction in dendritic cells
    Prafullakumar Tailor
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Res 16:134-40. 2006
  3. ncbi PLZF outreach: a finger in interferon's pie
    Keiko Ozato
    Program on Genomics in Differentiation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Immunity 30:757-8. 2009
  4. ncbi Another road to interferon: Yasuichi Nagano's journey
    Keiko Ozato
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892-2753, USA
    J Interferon Cytokine Res 27:349-52. 2007
  5. ncbi The interferon regulatory factor family in host defense: mechanism of action
    Keiko Ozato
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:20065-9. 2007
  6. ncbi Toll-like receptor signaling and regulation of cytokine gene expression in the immune system
    Keiko Ozato
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Biotechniques . 2002
  7. ncbi TRIM family proteins and their emerging roles in innate immunity
    Keiko Ozato
    Program of Genomics and Differentiation, National Institute of Child Health and Human Development, National Institutes of Health NIH, Bethesda, Maryland 20892 2753, USA
    Nat Rev Immunol 8:849-60. 2008
  8. ncbi Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription
    Anup Dey
    Laboratory of Molecular Growth Regulation, Program in Genomics of Differentiation, National Institute of Child Health and Human Development and Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 20:4899-909. 2009
  9. ncbi IFN regulatory factor-4 and -8 govern dendritic cell subset development and their functional diversity
    Tomohiko Tamura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 174:2573-81. 2005
  10. ncbi Gene disruption study reveals a nonredundant role for TRIM21/Ro52 in NF-kappaB-dependent cytokine expression in fibroblasts
    Ryusuke Yoshimi
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    J Immunol 182:7527-38. 2009

Detail Information

Publications75

  1. ncbi Brd4 is required for recovery from antimicrotubule drug-induced mitotic arrest: preservation of acetylated chromatin
    Akira Nishiyama
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Mol Biol Cell 17:814-23. 2006
    ..Brd4 plays an integral part in a cellular response to drug-induced mitotic stress by preserving a properly acetylated chromatin status...
  2. ncbi IRF family proteins and type I interferon induction in dendritic cells
    Prafullakumar Tailor
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Res 16:134-40. 2006
    ..IFN and other cytokines produced by activated DC in turn advance DC maturation and change the phenotype and function of DC. These processes are also likely to be governed by IRF family proteins...
  3. ncbi PLZF outreach: a finger in interferon's pie
    Keiko Ozato
    Program on Genomics in Differentiation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Immunity 30:757-8. 2009
    ..Interferon-induced PLZF phosphorylation and histone deacetylase 1 recruitment probably mediates the repressor-to-activator conversion...
  4. ncbi Another road to interferon: Yasuichi Nagano's journey
    Keiko Ozato
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892-2753, USA
    J Interferon Cytokine Res 27:349-52. 2007
  5. ncbi The interferon regulatory factor family in host defense: mechanism of action
    Keiko Ozato
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:20065-9. 2007
    ..This review deals with the diverse roles of IRFs in host defense and discusses the molecular mechanisms by which they regulate target gene transcription...
  6. ncbi Toll-like receptor signaling and regulation of cytokine gene expression in the immune system
    Keiko Ozato
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Biotechniques . 2002
    ..1). Together, although the basic backbone is conserved throughout evolution, the TLR signaling system in mammalian species has an added complexity to accommodate a mechanism that links innate and adaptive immunity...
  7. ncbi TRIM family proteins and their emerging roles in innate immunity
    Keiko Ozato
    Program of Genomics and Differentiation, National Institute of Child Health and Human Development, National Institutes of Health NIH, Bethesda, Maryland 20892 2753, USA
    Nat Rev Immunol 8:849-60. 2008
    ..In this Review, we describe recent data that reveal broader antiviral and antimicrobial activities of TRIM proteins and discuss their involvement in the regulation of pathogen-recognition and transcriptional pathways in host defence...
  8. ncbi Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription
    Anup Dey
    Laboratory of Molecular Growth Regulation, Program in Genomics of Differentiation, National Institute of Child Health and Human Development and Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 20:4899-909. 2009
    ..In sum, Brd4 marks M/G1 genes for transcriptional memory during mitosis, and upon exiting mitosis, this mark acts as a signal for initiating their prompt transcription in daughter cells...
  9. ncbi IFN regulatory factor-4 and -8 govern dendritic cell subset development and their functional diversity
    Tomohiko Tamura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 174:2573-81. 2005
    ..Together, IRF-4 and IRF-8 serve as a backbone of the molecular program regulating DC subset development and their functional diversity...
  10. ncbi Gene disruption study reveals a nonredundant role for TRIM21/Ro52 in NF-kappaB-dependent cytokine expression in fibroblasts
    Ryusuke Yoshimi
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    J Immunol 182:7527-38. 2009
    ....
  11. ncbi Selective recognition of acetylated histones by bromodomain proteins visualized in living cells
    Tomohiko Kanno
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 13:33-43. 2004
    ..Thus the recognition of histone acetylation code by bromodomains is selective, is involved in transcription, and potentially conveys transcriptional memory across cell divisions...
  12. ncbi The bromodomain protein Brd4 stimulates G1 gene transcription and promotes progression to S phase
    Kazuki Mochizuki
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892, USA
    J Biol Chem 283:9040-8. 2008
    ..Brd4 recruitment was low to absent at genes not affected by Brd4 shRNA. The results indicate that Brd4 stimulates G(1) gene expression by binding to multiple G(1) gene promoters in a cell cycle-dependent manner...
  13. ncbi ICSBP/IRF-8 inhibits mitogenic activity of p210 Bcr/Abl in differentiating myeloid progenitor cells
    Tomohiko Tamura
    Bldg 6, Rm 2A01, Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Dr MSC 2753, Bethesda, MD 20892 2753, USA
    Blood 102:4547-54. 2003
    ..These results indicate that ICSBP inhibits growth of Bcr/Abl-transformed myeloid progenitor cells by activating several genes that interfere with the c-Myc pathway...
  14. ncbi The sequestosome 1/p62 attenuates cytokine gene expression in activated macrophages by inhibiting IFN regulatory factor 8 and TNF receptor-associated factor 6/NF-kappaB activity
    Ji Young Kim
    Laboratory of Molecular Growth Regulation, Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 182:2131-40. 2009
    ..Together, p62 may provide a mechanism by which to control excessive inflammatory responses after macrophage activation...
  15. ncbi Cutting edge: IFN consensus sequence binding protein/IFN regulatory factor 8 drives the development of type I IFN-producing plasmacytoid dendritic cells
    Hideki Tsujimura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 170:1131-5. 2003
    ..ICSBP-restored DCs produced IFN-alpha and IL-12p40 in a DC subset-selective manner with the amounts comparable to those by +/+ DCs. Together, ICSBP is essential for early pDC development and final maturation of both pDCs and mDCs...
  16. ncbi Intracellular delivery of acetyl-histone peptides inhibits native bromodomain-chromatin interactions and impairs mitotic progression
    Akira Nishiyama
    Laboratory of Molecular Growth Regulation, Genomics and Differentiation Program, National Institutes of Child Health and Human Development, National Institutes of Health, Building 6, Room 2A01, 6 Center Drive, Bethesda, MD 20892 2753, United States
    FEBS Lett 582:1501-7. 2008
    ..Together, PTD-based delivery of histone tail peptides offers a novel means to study the mechanism and biological significance of bromodomain-chromatin interactions in vivo...
  17. ncbi Cutting edge: autoantigen Ro52 is an interferon inducible E3 ligase that ubiquitinates IRF-8 and enhances cytokine expression in macrophages
    Hee Jeong Kong
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892, USA
    J Immunol 179:26-30. 2007
    ..Together, Ro52 is an E3 ligase for IRF-8 that acts in a non-degradation pathway of ubiquitination, and contributes to the elicitation of innate immunity in macrophages...
  18. ncbi Gamma interferon triggers interaction between ICSBP (IRF-8) and TEL, recruiting the histone deacetylase HDAC3 to the interferon-responsive element
    Takeshi Kuwata
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 22:7439-48. 2002
    ..By associating with two different Ets family proteins, ICSBP exerts a dual function in IFN-gamma-dependent gene regulation in an immune system-specific manner...
  19. ncbi ICSBP/IRF-8 retrovirus transduction rescues dendritic cell development in vitro
    Hideki Tsujimura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health NIH, Bethesda, MD 20892, USA
    Blood 101:961-9. 2003
    ..Taken together, this study identifies ICSBP as a factor critical for both early differentiation and final maturation of DCs...
  20. ncbi Analysis of interleukin-21-induced Prdm1 gene regulation reveals functional cooperation of STAT3 and IRF4 transcription factors
    Hyokjoon Kwon
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA
    Immunity 31:941-52. 2009
    ..These results reveal broad cooperative gene regulation by STAT3 and IRF4...
  21. ncbi Essential role for ICSBP in the in vivo development of murine CD8alpha + dendritic cells
    Julio Aliberti
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:305-10. 2003
    ..Together these results demonstrate that ICSBP is critically required for the in vivo differentiation of CD8alpha(+) DCs and may also influence the functional maturation of the CD8alpha(-) subsets...
  22. ncbi Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages
    Leopoldo Laricchia-Robbio
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Proc Natl Acad Sci U S A 102:14368-73. 2005
    ..1 and/or IRF1, the mobility of IRF8 was markedly reduced, producing a more stably bound component. Together, IRF8-chromatin interaction is dynamic in live macrophages and influenced by partner proteins and immunological stimuli...
  23. ncbi Modulation of the Brd4/P-TEFb interaction by the human T-lymphotropic virus type 1 tax protein
    Won Kyung Cho
    Virus Tumor Biology Section, Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 41 Medlars Dr, Bldg 41, Rm B201, Bethesda, MD 20892, USA
    J Virol 81:11179-86. 2007
    ..Our results suggest the possibility that Tax may compete and functionally substitute for Brd4 in P-TEFb regulation...
  24. ncbi The feedback phase of type I interferon induction in dendritic cells requires interferon regulatory factor 8
    Prafullakumar Tailor
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Immunity 27:228-39. 2007
    ..Together, these data indicate that IRF8 magnifies the second phase of IFN transcription in DCs by prolonging binding of basic transcription machinery to the IFN promoters, thereby playing a role in innate immunity...
  25. ncbi CpG-activated Thy1.2+ dendritic cells protect against lethal Listeria monocytogenes infection
    Ken J Ishii
    Section of Retroviral Immunology, Center for Biologics Evaluation Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Eur J Immunol 35:2397-405. 2005
    ..2-) or plasmacytoid DC (mPDCA+), and secrete IFN-gamma that contributes to protection. These findings suggest that a novel Thy1.2+ DC subset plays a critical role in mediating the immunoprotective activity of CpG DNA...
  26. ncbi Inducible deposition of the histone variant H3.3 in interferon-stimulated genes
    Tomohiko Tamura
    Laboratory of Molecular Growth Regulation, Eunice Kennedy Shriver NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:12217-25. 2009
    ..3 knockdown cells. Results indicate that H3.3 plays a role in IFN-mediated transcription, and its deposition leaves a prolonged post-transcriptional mark in these genes...
  27. ncbi Bromodomain protein Brd4 binds to GTPase-activating SPA-1, modulating its activity and subcellular localization
    Andrea Farina
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Mol Cell Biol 24:9059-69. 2004
    ..This work reveals a novel link between Brd4 and a GTPase-dependent mitogenic signaling pathway...
  28. ncbi Toll-like receptor 9 signaling activates NF-kappaB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells
    Hideki Tsujimura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:6820-7. 2004
    ..IRF-8 reintroduction fully restored CpG activation of NF-kappaB and cytokine induction in -/- DCs. Together, TLR signals that activate NF-kappaB are diverse among different TLRs, and TLR9 signaling uniquely depends on IRF-8 in DCs...
  29. ncbi Deletion of the proline-rich region of the murine metastasis susceptibility gene Brd4 promotes epithelial-to-mesenchymal transition- and stem cell-like conversion
    Jude Alsarraj
    Laboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Cancer Res 71:3121-31. 2011
    ..Thus our findings suggest that BRD4 may be altering the predisposition of tumors to undergo conversion to a more de-differentiated or primitive state during metastatic progression...
  30. ncbi The interferon regulatory factor ICSBP/IRF-8 in combination with PU.1 up-regulates expression of tumor suppressor p15(Ink4b) in murine myeloid cells
    Martina Schmidt
    Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892 4263, USA
    Blood 103:4142-9. 2004
    ....
  31. ncbi The BXH2 mutation in IRF8 differentially impairs dendritic cell subset development in the mouse
    Prafullakumar Tailor
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Blood 111:1942-5. 2008
    ..Together, this work indicates that IRF8-partner interactions play different roles in CD8alpha(+) DCs and pDCs, revealing a mechanistic separation that underlies development of these DC subsets...
  32. ncbi Interaction of bovine papillomavirus E2 protein with Brd4 stabilizes its association with chromatin
    Maria G McPhillips
    Laboratory of Viral Diseases, NIAID, NIH, Building 4, Room 137, 4 Center Dr, MSC 0455, Bethesda, MD 20892-0455, USA
    J Virol 79:8920-32. 2005
    ..This study demonstrates that the segregation of papillomavirus genomes is not simply due to the passive hitchhiking of the E2/genome complex with a convenient cellular chromosomal protein...
  33. ncbi Identification of target genes and a unique cis element regulated by IRF-8 in developing macrophages
    Tomohiko Tamura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Dr, MSC 2753, Bethesda, MD 20892 2753, USA
    Blood 106:1938-47. 2005
    ..1 Ets transcription factor bind to this element in vivo. Collectively, these data indicate that IRF-8 stimulates transcription of target genes through a novel cis element to specify macrophage differentiation...
  34. ncbi The mitotic chromosome binding activity of the papillomavirus E2 protein correlates with interaction with the cellular chromosomal protein, Brd4
    Michael K Baxter
    Laboratory of Viral Diseases, NIAID, NIH, Building 4, Room 137, 4 Center Dr, MSC 0455, Bethesda, MD 20892 0455, USA
    J Virol 79:4806-18. 2005
    ....
  35. ncbi Developmental and epigenetic regulation of the human TLR3 gene
    Analia Porrás
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, MD 20892, United States
    Mol Immunol 46:27-36. 2008
    ..These findings reveal a new source for variability in innate immune system function and provide a model for further study of perinatal epigenetic transitions during development...
  36. ncbi The double bromodomain protein Brd4 binds to acetylated chromatin during interphase and mitosis
    Anup Dey
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:8758-63. 2003
    ..This colocalization also required both bromodomains. These observations indicate that Brd4 specifically recognizes acetylated histone codes, and this recognition is passed onto the chromatin of newly divided cells...
  37. ncbi Muramyl dipeptide activation of nucleotide-binding oligomerization domain 2 protects mice from experimental colitis
    Tomohiro Watanabe
    Mucosal Immunity Section, Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 118:545-59. 2008
    ....
  38. ncbi Ebola Zaire virus blocks type I interferon production by exploiting the host SUMO modification machinery
    Tsung Hsien Chang
    Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 5:e1000493. 2009
    ....
  39. ncbi Interaction of histone acetylases and deacetylases in vivo
    Satoshi Yamagoe
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 23:1025-33. 2003
    ..This HDAC-HAT association is partly accounted for by a direct protein-protein interaction observed in vitro. The HDAC-HAT complex may play a role in establishing a dynamic equilibrium of the two enzymes in vivo...
  40. ncbi The chromatin-remodeling BAF complex mediates cellular antiviral activities by promoter priming
    Kairong Cui
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 24:4476-86. 2004
    ..We propose that constitutive binding of the BAF complex is an important mechanism for the IFN-inducible promoters to respond rapidly to IFN and virus stimulation...
  41. ncbi Virus-induced differential expression of nuclear receptors and coregulators in dendritic cells: implication to interferon production
    Sinnie Sin Man Ng
    Unit on Molecular Hormone Action, Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1109, USA
    FEBS Lett 585:1331-7. 2011
    ..Thus, NRs and coregulators are integral components of DC-organizing anti-viral response wherein NOR1 and LXR? participate in regulating interferon production...
  42. ncbi Induction of an anti-inflammatory cytokine, IL-10, in dendritic cells after toll-like receptor signaling
    Ranmal Samarasinghe
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Interferon Cytokine Res 26:893-900. 2006
    ..TLR-stimulated IL-10 production may regulate DC maturation steps, thereby influencing the ensuing immune responses...
  43. ncbi Proteomic survey of ubiquitin-linked nuclear proteins in interferon-stimulated macrophages
    Ji Young Kim
    Program of Genomics in Differentiation, Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland, USA
    J Interferon Cytokine Res 31:619-28. 2011
    ....
  44. ncbi Bromodomain 4 activation predicts breast cancer survival
    Nigel P S Crawford
    Laboratory of Cancer Biology and Genetics and Laboratory of Population Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:6380-5. 2008
    ..Taken together, these data suggest that dysregulation of Brd4-associated pathways may play an important role in breast cancer progression and underlies multiple common prognostic signatures...
  45. ncbi Brd4 associates with mitotic chromosomes throughout early zebrafish embryogenesis
    Reiko Toyama
    Laboratory of Molecular Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, Maryland, USA
    Dev Dyn 237:1636-44. 2008
    ..The association of Brd4 with acetylated histones may also be conserved in early embryos as we found that histones H3 and H4 are already acetylated during pre-MBT stages...
  46. ncbi Immune cell-specific amplification of interferon signaling by the IRF-4/8-PU.1 complex
    Yuka Kanno
    Molecular Immunology and Inflammation Branch, National Institute of Arthritis, Muscuolskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Interferon Cytokine Res 25:770-9. 2005
    ..1complex provides secondary IFN signaling pathways unique to the immune system. Collectively, the contribution of IRF-8 and PU.1 to IFN-regulated gene expression may in part account for immune cell-specific functions of IFNs...
  47. ncbi Mechanistic link between PKR dimerization, autophosphorylation, and eIF2alpha substrate recognition
    Madhusudan Dey
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 122:901-13. 2005
    ..We propose an ordered mechanism of PKR activation in which catalytic-domain dimerization triggers Thr446 autophosphorylation and specific eIF2alpha substrate recognition...
  48. ncbi IFN consensus sequence binding protein/IFN regulatory factor-8 guides bone marrow progenitor cells toward the macrophage lineage
    Hideki Tsujimura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 169:1261-9. 2002
    ..Taken together, ICSBP plays a critical role in myeloid cell development by controlling lineage selection and is indispensable for IFN-gamma-dependent modulation of progenitor cell maturation...
  49. ncbi A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase
    Tetsuo Maruyama
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Mol Cell Biol 22:6509-20. 2002
    ..Taken as a whole, the present study suggests that Brd4 regulates cell cycle progression in part by interacting with RFC...
  50. ncbi The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription
    Moon Kyoo Jang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 19:523-34. 2005
    ..Together, P-TEFb alternately interacts with Brd4 and the inhibitory subunit to maintain functional equilibrium in the cell...
  51. ncbi ICSBP/IRF-8: its regulatory roles in the development of myeloid cells
    Tomohiko Tamura
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Interferon Cytokine Res 22:145-52. 2002
    ..These results illustrate the mechanism by which the loss of ICSBP leads to immunodeficiency and CML-like syndrome and suggest ICSBP's critical role in the development of myeloid cells...
  52. ncbi Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexes
    Jung S Byun
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:19286-91. 2009
    ....
  53. ncbi Chromosomal integration of retinoic acid response elements prevents cooperative transcriptional activation by retinoic acid receptor and retinoid X receptor
    Bruno Lefebvre
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 22:1446-59. 2002
    ....
  54. ncbi Phosphorylation of histone H3 is functionally linked to retinoic acid receptor beta promoter activation
    Bruno Lefebvre
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO Rep 3:335-40. 2002
    ..Since such post-translational modifications were not observed at several other promoters, we conclude that histone H3 phosphorylation may be a molecular signature of the activated, retinoid-controlled mRARbeta2 gene promoter...
  55. ncbi Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes
    Jianxin You
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 117:349-60. 2004
    ..The interaction of E2 with Brd4 is required to ensure the tethering of viral genomes to the host mitotic chromosomes for persistence of viral episomes in PV-infected cells...
  56. ncbi Synergistic activation of interleukin-12 p35 gene transcription by interferon regulatory factor-1 and interferon consensus sequence-binding protein
    Jianguo Liu
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    J Biol Chem 279:55609-17. 2004
    ..This study provides novel mechanistic information on how signals initiated during innate and adaptive immune responses synergize to yield greater IL-12 production and sustained cellular immunity...
  57. ncbi Ubiquitin-dependent degradation of interferon regulatory factor-8 mediated by Cbl down-regulates interleukin-12 expression
    Huabao Xiong
    Immunobiology Center, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 280:23531-9. 2005
    ..Taken together, these results suggest that the proteasomal degradation of IRF-8 mediated by the ubiquitin E3 ligase Cbl down-regulates IL-12 expression...
  58. ncbi The role of IFN regulatory factor-3 in the cytotoxic activity of NS-9, a polyinosinic-polycytidylic acid/cationic liposome complex, against tumor cells
    Tomonori Uno
    Discovery Research Laboratories, Nippon Shinyaku Co, Ltd, 14 Nishinosho Monguchi cho, Kisshoin, Minami, Kyoto 601 8550, Japan
    Mol Cancer Ther 4:799-805. 2005
    ..We conclude that IRF3 plays a crucial role in the cytotoxic activity of NS-9 against tumor cells, whereas RNA-dependent protein kinase, RNase L, or type I IFNs are not important for its activity...
  59. ncbi Nramp1-mediated innate resistance to intraphagosomal pathogens is regulated by IRF-8, PU.1, and Miz-1
    Michal Alter-Koltunoff
    Department of Food Engineering and Biotechnology, Technion, Haifa 32000, Israel
    J Biol Chem 278:44025-32. 2003
    ..Thus, our results explain in molecular terms the role of IRF-8 in conferring innate resistance to intracellular pathogens and point to its possible involvement in autoimmune diseases...
  60. ncbi Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression
    Toru Kubota
    Department of Virology III, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashi Murayama, Tokyo, Japan
    J Biol Chem 283:25660-70. 2008
    ..Together, SUMO modification is an integral part of IRF3 and IRF7 activity that contributes to postactivation attenuation of IFN production...
  61. ncbi IRF-8/interferon (IFN) consensus sequence-binding protein is involved in Toll-like receptor (TLR) signaling and contributes to the cross-talk between TLR and IFN-gamma signaling pathways
    Jie Zhao
    Immunobiology Center, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Biol Chem 281:10073-80. 2006
    ..Taken together, the results suggest that the interaction of IRF-8 with TRAF6 modulates TLR signaling and may contribute to the cross-talk between IFN-gamma and TLR signal pathways...
  62. ncbi Interferon regulatory factor-2 regulates cell growth through its acetylation
    Atsuko Masumi
    Department of Safety Research on Biologics, National Institute of Infectious Diseases, Tokyo, Japan
    J Biol Chem 278:25401-7. 2003
    ..These results indicate that IRF-2 is acetylated in a cell growth-dependent manner, which enables it to contribute to transcription of cell growth-regulated promoters...
  63. ncbi Crystal structure of the human BRD2 bromodomain: insights into dimerization and recognition of acetylated histone H4
    Yoshihiro Nakamura
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    J Biol Chem 282:4193-201. 2007
    ..The two acetyllysine-binding pockets and a negatively charged secondary binding pocket, produced at the dimer interface in BRD2 BD1, may be the unique features that allow BRD2 BD1 to selectively bind to the acetylated H4 tail...
  64. ncbi Interferon regulatory factor-8 is indispensable for the expression of promyelocytic leukemia and the formation of nuclear bodies in myeloid cells
    Natalie Dror
    Department of Biotechnology and Food Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 282:5633-40. 2007
    ..When IRF-8 levels are compromised, the reduced PML expression may lead to genome instability and eventually to the leukemic phenotype...
  65. ncbi Histone acetylation and subcellular localization of chromosomal protein BRD4 during mouse oocyte meiosis and mitosis
    Takashi Nagashima
    Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan
    Mol Hum Reprod 13:141-8. 2007
    ....
  66. ncbi Natural killer dendritic cells are an intermediate of developing dendritic cells
    Li Chen
    Department of Pathology, Ohio State University Medical Center, 129 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    J Leukoc Biol 81:1422-33. 2007
    ..1(+)MHC II(-) and possess strong cytotoxic function yet show a poor ability to present antigen in the steady state. These findings suggest that NKDCs may play a critical role in linking innate and adaptive immunity...
  67. ncbi Innate immunity to intraphagosomal pathogens is mediated by interferon regulatory factor 8 (IRF-8) that stimulates the expression of macrophage-specific Nramp1 through antagonizing repression by c-Myc
    Michal Alter-Koltunoff
    Department of Biotechnology and Food Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 283:2724-33. 2008
    ..Thus, interplay between repression and activation state of the Nramp1 promoter mediated by IRF-8 provides the molecular basis by which macrophages resist intraphagosomal pathogens at early stage after infection...
  68. ncbi Complex formation of the interferon (IFN) consensus sequence-binding protein with IRF-1 is essential for murine macrophage IFN-gamma-induced iNOS gene expression
    Huabao Xiong
    Immunobiology Center, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 278:2271-7. 2003
    ..Complex formation of ICSBP with IRF-1 is essential for iNOS expression, and interleukin-4 attenuates the physical interaction of ICSBP with IRF-1 resulting in the inhibition of INOS gene expression...
  69. ncbi IRF-8/ICSBP and IRF-1 cooperatively stimulate mouse IL-12 promoter activity in macrophages
    Atsuko Masumi
    Department of Safety Research on Biologics, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama shi, Tokyo, Japan
    FEBS Lett 531:348-53. 2002
    ..Together, these results indicate that ICSBP and IRF-1 cooperatively stimulate murine IL-12 transcription through a novel regulatory element in the murine promoter...
  70. ncbi The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA
    Vladimir P Bermudez
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:10237-42. 2003
    ..These results support a model in which sister chromatid cohesion is linked to DNA replication...
  71. ncbi Global nature of dynamic protein-chromatin interactions in vivo: three-dimensional genome scanning and dynamic interaction networks of chromatin proteins
    Robert D Phair
    BioInformatics Services, Rockville, MD 20854, USA
    Mol Cell Biol 24:6393-402. 2004
    ..We suggest that these properties are crucial for generating high plasticity in genome expression...
  72. ncbi Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4
    Zhiyuan Yang
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Mol Cell 19:535-45. 2005
    ..Brd4, HEXIM1, and 7SK are all implicated in regulating cell growth, which may result from their dynamic control of the general transcription factor P-TEFb...
  73. ncbi Identification of IRF-8 and IRF-1 target genes in activated macrophages
    Natalie Dror
    Department of Biotechnology and Food Engineering, Technion Israel Institute of Technology, Haifa, Israel
    Mol Immunol 44:338-46. 2007
    ..We therefore suggest a broader role for IRF-1 and IRF-8 in macrophages differentiation and maturation, being important inflammatory mediators...
  74. ncbi The role of the interferon regulatory factor (IRF) family in dendritic cell development and function
    Lucia Gabriele
    Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Cytokine Growth Factor Rev 18:503-10. 2007
    ....
  75. ncbi Repression of IFN regulatory factor 8 by DNA methylation is a molecular determinant of apoptotic resistance and metastatic phenotype in metastatic tumor cells
    Dafeng Yang
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia, USA
    Cancer Res 67:3301-9. 2007
    ....