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Genomes and Genes | M DassoSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Ran-GTP regulates kinetochore attachment in somatic cellsAlexei Arnaoutov
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, Maryland 20892-5431, USA
Cell Cycle 4:1161-5. 2005..Here we discuss potential implications of these findings for normal k-fiber assembly...- Distinct in vivo dynamics of vertebrate SUMO paraloguesFerhan Ayaydin
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
Mol Biol Cell 15:5208-18. 2004.... - Crm1 is a mitotic effector of Ran-GTP in somatic cellsAlexei Arnaoutov
Laboratory of Gene Regulation and Development, NICHD, NIH, Building 18, Room 106, Bethesda, MD 20892 5431, USA
Nat Cell Biol 7:626-32. 2005..Thus, Crm1 is a critical Ran-GTP effector for mitotic spindle assembly and function in somatic cells... - Cell biology: new cog for a familiar machineMary Dasso
Nature 435:899-900. 2005 - Emerging roles of the SUMO pathway in mitosisMary Dasso
Laboratory of Gene Regulation and Development, NICHD NIH, Building 18, Room 106, MSC 5431, Bethesda, MD 20892, USA
Cell Div 3:5. 2008..Finally, growing evidence suggests that SUMOylation is a downstream target of regulation through Ran, a small GTPase with important functions in both interphase nuclear trafficking and mitotic spindle assembly... - The Ran GTPase: theme and variationsMary Dasso
Laboratory of Gene Regulation and Development, NICHD NIH, Bethesda, MD 20892 5431, USA
Curr Biol 12:R502-8. 2002..Our emergent understanding of Ran suggests that it plays a central role in spatial and temporal organization of the vertebrate cell... - Ran at kinetochoresM Dasso
Laboratory of Gene Regulation and Development, NICHD National Institute of Child Health and Human Development NIH National Institutes of Health, Bethesda, MD 20892 5431, USA
Biochem Soc Trans 34:711-5. 2006..Here, I will briefly summarize evidence for these kinetochore-associated functions and mention some of the issues that remain to be addressed regarding them... - The balance of RanBP1 and RCC1 is critical for nuclear assembly and nuclear transportR T Pu
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5431, USA
Mol Biol Cell 8:1955-70. 1997..The results further suggest that the balance of GTP- and GDP-Ran is critical for proper nuclear assembly and function in vitro... - Ubc9p and the conjugation of SUMO-1 to RanGAP1 and RanBP2H Saitoh
Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Building 18, Room 106, Bethesda, Maryland 20892 5431, USA
Curr Biol 8:121-4. 1998..We also show that p18(Ubc9) interacts specifically with the internal repeat domain of RanBP2, which is a substrate for SUMO-1 conjugation in Xenopus egg extracts... - The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extractsH Saitoh
Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
J Biol Chem 270:10658-63. 1995..The fourth protein is novel and has a molecular mass of 340 kDa. In this report, we discuss a preliminary characterization of the interactions between these proteins... - The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A)+ RNAA Bharathi
Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Gene 198:251-8. 1997..An epitope tagged human Rae1p localizes to both the nucleus and the cytoplasm in transiently transfected HeLa cells. We discuss the potential role of Rae1p in nuclear cytoplasmic trafficking in yeast and higher eukaryotic cells... - The promyelocytic leukemia protein stimulates SUMO conjugation in yeastB B Quimby
Section on Cell Cycle Regulation, Laboratory of Gene Regulation and Development, NICHD NIH, Bethesda, MD 20892, USA
Oncogene 25:2999-3005. 2006..These findings point toward a potential function of PML and PML:RARalpha as SUMO E3 enzymes or E3 regulators, and suggest that fusion of RARalpha to PML may affect this activity... - Expression and regulation of the mammalian SUMO-1 E1 enzymeY Azuma
Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Bethesda, Maryland 20892-5431, USA
FASEB J 15:1825-7. 2001 - The fate of metaphase kinetochores is weighed in the balance of SUMOylation during S phaseDebaditya Mukhopadhyay
Laboratory of Gene Regulation and Development, Bethesda, MD, USA
Cell Cycle 9:3194-201. 2010..Here, we will briefly review the implications of our findings and present new data on how SUMOylation during S phase can control chromosome alignment in the subsequent metaphase... - Ran GTPase regulates Mad2 localization to the nuclear pore complexB Booth Quimby
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
Eukaryot Cell 4:274-80. 2005..This is the first evidence indicating that Ran affects the localization of Mad2p to the NPC... - The Ran GTPase regulates kinetochore functionAlexei Arnaoutov
Laboratory of Gene Regulation and Development, NICHD, NIH, Building 18, Room 106, 20892, Bethesda, MD, USA
Dev Cell 5:99-111. 2003..Finally, we observe a clear wave of RCC1 association to mitotic chromosomes at the metaphase-anaphase transition in normal cycling extracts, suggesting that this mechanism has an important role in unperturbed cell cycles... - The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochoresRam Kumar Mishra
Laboratory of Gene Regulation and Development, NICHD NIH, Bethesda, MD 20892, USA
Nat Cell Biol 12:164-9. 2010.... - Meeting report: International Symposium on 'Ran and the Cell Cycle', October 2-5, 2005, Awaji Yumebutai, JapanMary Dasso
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892-5431, USA
Traffic 7:474-8. 2006 - RCC1 in the cell cycle: the regulator of chromosome condensation takes on new rolesM Dasso
Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Bethesda, MD 20892
Trends Biochem Sci 18:96-101. 1993.... - RanBP2 associates with Ubc9p and a modified form of RanGAP1H Saitoh
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
Proc Natl Acad Sci U S A 94:3736-41. 1997..These results suggest Ubc9p's role in cell cycle regulation may involve either modification of nuclear transport substrates or the nuclear transport machinery... - The SUMO protease SENP6 is essential for inner kinetochore assemblyDebaditya Mukhopadhyay
Laboratory of Gene Regulation and Development, National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 188:681-92. 2010..Together, these findings reveal a novel mechanism whereby the finely balanced activities of SENP6 and RNF4 control vertebrate kinetochore assembly through SUMO-targeted destabilization of inner plate components... - Bub1 is essential for assembly of the functional inner centromereYekaterina Boyarchuk
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 176:919-28. 2007..We further provide evidence that Bub1 kinase activity is pivotal for recruitment of all of these components. Together, our findings demonstrate that Bub1 acts at multiple points to assure the correct kinetochore formation... - The small GTPase Ran: interpreting the signsB Booth Quimby
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development/National Institutes of Health, Building 18T, Room 106, 18 Library Drive, Bethesda, MD 20892-5431, USA
Curr Opin Cell Biol 15:338-44. 2003..Moreover, recent experimental evidence has documented the distribution of Ran-GTP within cells and supported the notion that Ran plays a central role in the spatial and temporal organization of the eukaryotic cell... - SUMO-1 targets RanGAP1 to kinetochores and mitotic spindlesJomon Joseph
Laboratory of Gene Regulation and Development, National Institute of Child Health and Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 156:595-602. 2002.... - Association of the human SUMO-1 protease SENP2 with the nuclear poreJun Hang
Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892 5431, USA
J Biol Chem 277:19961-6. 2002..Our results suggest that association with the pore plays an important negative role in the regulation of SENP2, perhaps by restricting its activity to a subset of the conjugated proteins within the nucleus... - PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomesYoshiaki Azuma
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, MD 20892 5431, USA
EMBO J 24:2172-82. 2005.... - Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteasesChawon Yun
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
J Cell Biol 183:589-95. 2008..Together, these results suggest that regulation of SUMO deconjugation may be a major facet of B23/nucleophosmin function in vivo... - A new clue at the nuclear pore: RanBP2 is an E3 enzyme for SUMO1Yoshiaki Azuma
Laboratory of Gene Regulation and Development, NICHD/NIH, Building 18, Room 106, Bethesda, MD 20892, USA
Dev Cell 2:130-1. 2002..These intriguing results raise important questions about the mechanism of SUMO1 conjugation, the relationship of SUMO1 to nuclear transport, and the regulation of RanBP2 in the pore... - Modification in reverse: the SUMO proteasesDebaditya Mukhopadhyay
Laboratory of Gene Regulation and Development, National Institute of Child Health and Development, National Institutes of Health, Building 18, Room 106, Bethesda, MD 20892, USA
Trends Biochem Sci 32:286-95. 2007..The dissimilar sub-nuclear localization patterns of Ulp/SENPs and phenotypes of Ulp/SENP mutants further indicate that different Ulp/SENPs have distinct and non-redundant roles... - SUSP1 antagonizes formation of highly SUMO2/3-conjugated speciesDebaditya Mukhopadhyay
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 174:939-49. 2006..Together, our findings argue that SUSP1 may play a specialized role in dismantling highly conjugated SUMO2 and -3 species that is critical for PML body maintenance... - Potential roles of the nucleotide exchange factor ECT2 and Cdc42 GTPase in spindle assembly in Xenopus egg cell-free extractsTakashi Tatsumoto
Molecular Tumor Biology Section, Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Cell Biochem 90:892-900. 2003..These results suggest that the Rho family GTPase Cdc42 and its exchange factor XECT2 are critical regulators of spindle assembly in Xenopus egg extracts... - An ent-kaurene that inhibits mitotic chromosome movement and binds the kinetochore protein ran-binding protein 2Natalie T Rundle
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
ACS Chem Biol 1:443-50. 2006..Mass spectrometric analysis identified six EKA-binding proteins, including Ran-binding protein 2, a kinetochore protein whose depletion by small interfering RNA causes a similar mitotic arrest phenotype... - Nuclear transport erupts on the slopes of Mount EtnaMaureen A Powers
Department of Cell Biology, Emory University School of Medicine, 455 Whitehead Building, 615 Michael Street, Atlanta, GA, 30322, USA
Nat Cell Biol 6:82-6. 2004..Presentations emphasized the dynamic properties of the nuclear trafficking machinery, and demonstrated the continuity of nuclear transport with processes in the nucleus and cytoplasm... - The Nup107-160 nucleoporin complex is required for correct bipolar spindle assemblyArturo V Orjalo
Sections of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego Medical School, La Jolla, CA 92093 0347, USA
Mol Biol Cell 17:3806-18. 2006..We conclude that the Nup107-160 complex is dynamic in mitosis and that it promotes spindle assembly in a manner that is distinct from its functions at interphase nuclear pores... - Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modulesAmy J Prunuske
Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
Mol Biol Cell 17:760-9. 2006..These results provide new information about the critical components of nuclear membrane remodeling and lay the foundation for a better understanding of how this process is regulated... - The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivoJomon Joseph
National Institutes of Health, National Institute of Child Health and Human Development, Building 18, Room 106, 18 Library Drive, MSC 5431, Bethesda, MD 20892, USA
Curr Biol 14:611-7. 2004..Taken together, our observations indicate that RanBP2 and RanGAP1 are targeted as a single complex that is both regulated by and essential for stable kinetochore-MT association... - SUMO-2/3 regulates topoisomerase II in mitosisYoshiaki Azuma
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5431, USA
J Cell Biol 163:477-87. 2003..Together, our results suggest that SUMO conjugation is important for chromosome segregation in metazoan systems, and that mobilization of topoisomerase II from mitotic chromatin may be a key target of this modification... - Plant-specific mitotic targeting of RanGAP requires a functional WPP domainSun Yong Jeong
Plant Biotechnology Center and Department of Plant Cellular and Molecular Biology, The Ohio State University, Columbus, OH 43210, USA
Plant J 42:270-82. 2005..These results indicate that plant and animal RanGAPs undergo different migration patterns during cell division, which require their kingdom-specific targeting domains...