Research Topics
| Christopher K RaymondSummaryAffiliation: Merck Research Laboratories Country: USA Publications
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Detail Information
Publications
Expression of alternatively spliced sodium channel alpha-subunit genes. Unique splicing patterns are observed in dorsal root gangliaChristopher K Raymond
Rosetta Inpharmatics LLC, Seattle, Washington 98109, USA
J Biol Chem 279:46234-41. 2004..Consistent down-regulation of all transcripts was observed, as well as significant changes in the splicing patterns of SCN8A and SCN9A...- Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAsChristopher K Raymond
Rosetta Inpharmatics, Seattle, WA 98109, USA
RNA 11:1737-44. 2005..We used the method for quantitative analysis of six microRNAs across 12 tissue samples. The data confirm striking variation in the patterns of expression of these noncoding regulatory RNAs... - Genetic validation of whole-transcriptome sequencing for mapping expression affected by cis-regulatory variationTomas Babak
Rosetta Inpharmatics, LLC, 401 Terry Ave N, Seattle, WA 98109, USA
BMC Genomics 11:473. 2010.... - NSR-seq transcriptional profiling enables identification of a gene signature of Plasmodium falciparum parasites infecting childrenMarissa Vignali
Seattle Biomedical Research Institute, Seattle, Washington, USA
J Clin Invest 121:1119-29. 2011..Our study provides proof of principle that NSR-seq represents an approach that can be used to study clinical isolates of parasites causing severe malaria syndromes as well other blood-borne pathogens and blood-related diseases... - ACC2 is expressed at high levels in human white adipose and has an isoform with a novel N-terminus [corrected]John C Castle
Rosetta Inpharmatics LLC, Seattle, Washington, United States of America
PLoS ONE 4:e4369. 2009..The results demonstrate a significant difference in ACC expression between human and rodents, which may introduce difficulties for the use of rodent models for development of ACC inhibitors... - DNA copy number, including telomeres and mitochondria, assayed using next-generation sequencingJohn C Castle
Rosetta Inpharmatics LLC, Merck and Co, Inc, Seattle, Washington 98109, USA
BMC Genomics 11:244. 2010..DNA copy number variations occur within populations and aberrations can cause disease. We sought to develop an improved lab-automatable, cost-efficient, accurate platform to profile DNA copy number... - Digital transcriptome profiling using selective hexamer priming for cDNA synthesisChristopher D Armour
Department of Molecular Informatics, Rosetta Inpharmatics LLC, a wholly owned subsidiary of Merck and Co, Inc, Seattle, Washington, USA
Nat Methods 6:647-9. 2009..In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing... - Activity of the Rhodopseudomonas palustris p-coumaroyl-homoserine lactone-responsive transcription factor RpaRHidetada Hirakawa
Department of Microbiology, University of Washington, Seattle, Washington 98195 7242, USA
J Bacteriol 193:2598-607. 2011..These data raise the possibility that this antisense RNA or other RpaR-activated noncoding RNAs mediate the indirect activation of genes in the RpaR-controlled regulon... - Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progressionPeter S Linsley
Rosetta Inpharmatics, LLC, 401 Terry Ave N, Seattle, WA 98109, USA
Mol Cell Biol 27:2240-52. 2007..Simultaneous silencing of these genes was more effective at blocking cell cycle progression than disruption of the individual genes. Thus, miR-16 coordinately regulates targets that may act in concert to control cell cycle progression... - Targeted, haplotype-resolved resequencing of long segments of the human genomeChristopher K Raymond
University of Washington Genome Center, Department of Medicine, University of Washington, Seattle, WA 98195, USA
Genomics 86:759-66. 2005..We illustrate its implementation with a detailed example of more than 400 kbp from the human CFTR region, across 15 individuals, and summarize our experience applying it to many other human loci... - Genetic variation at the O-antigen biosynthetic locus in Pseudomonas aeruginosaChristopher K Raymond
University of Washington Genome Center, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
J Bacteriol 184:3614-22. 2002..The overall pattern underscores the plasticity of the P. aeruginosa genome, in which a specific site in a well-conserved genomic region can be occupied by any of numerous islands of functionally related DNA with diverse sequences... - Linker-mediated recombinational subcloning of large DNA fragments using yeastChristopher K Raymond
The University of Washington Genome Center, Department of Medicine, University of Washington, Seattle, Washington 98115, USA
Genome Res 12:190-7. 2002..This technology may find broad application in the final stages of completing the Human Genome Sequencing Project and in applications of BAC clones to the functional analysis of complex genomes... - The colorectal microRNAomeJordan M Cummins
The Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA
Proc Natl Acad Sci U S A 103:3687-92. 2006..These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues...