DAVID ROBERT BARNIDGE

Summary

Affiliation: Mayo Clinic
Country: USA

Publications

  1. ncbi Protein expression profiling of CLL B cells using replicate off-line strong cation exchange chromatography and LC-MS/MS
    David R Barnidge
    Mayo Proteomics Research Center, Mayo Clinic College of Medicine, 200 1st Street SW, Rochester, MN 55905, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 819:33-9. 2005
  2. ncbi Absolute quantification of the G protein-coupled receptor rhodopsin by LC/MS/MS using proteolysis product peptides and synthetic peptide standards
    David R Barnidge
    Neuromics Incorporated, Minneapolis, Minnesota 55414, USA
    Anal Chem 75:445-51. 2003
  3. ncbi Quantitative protein expression analysis of CLL B cells from mutated and unmutated IgV(H) subgroups using acid-cleavable isotope-coded affinity tag reagents
    David R Barnidge
    Mayo Proteomics Research Center, Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Proteome Res 4:1310-7. 2005

Research Grants

  1. B-CLL Proteins Quantified by Mass Spectrometry
    DAVID BARNIDGE; Fiscal Year: 2004

Detail Information

Publications3

  1. ncbi Protein expression profiling of CLL B cells using replicate off-line strong cation exchange chromatography and LC-MS/MS
    David R Barnidge
    Mayo Proteomics Research Center, Mayo Clinic College of Medicine, 200 1st Street SW, Rochester, MN 55905, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 819:33-9. 2005
    ..We show that despite the variance in SCX, capillary LC, and the data-dependent selection of precursor ions, an overlap of 64% between proteins identified in replicate runs was achieved for this system...
  2. ncbi Absolute quantification of the G protein-coupled receptor rhodopsin by LC/MS/MS using proteolysis product peptides and synthetic peptide standards
    David R Barnidge
    Neuromics Incorporated, Minneapolis, Minnesota 55414, USA
    Anal Chem 75:445-51. 2003
    ..We demonstrate that quantification is simple and reliable for the integral membrane protein rhodopsin with reasonable recoveries for replicate experiments using low-micromolar solutions of rhodopsin from rod outer segments...
  3. ncbi Quantitative protein expression analysis of CLL B cells from mutated and unmutated IgV(H) subgroups using acid-cleavable isotope-coded affinity tag reagents
    David R Barnidge
    Mayo Proteomics Research Center, Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Proteome Res 4:1310-7. 2005
    ..These results demonstrate that stable isotope labeling and mass spectrometry can complement 2D gel electrophoresis and gene microarray technologies for identifying putative and perhaps unique prognostic markers in CLL...

Research Grants1

  1. B-CLL Proteins Quantified by Mass Spectrometry
    DAVID BARNIDGE; Fiscal Year: 2004
    ..As part of this proposal the candidate will take courses offered in the Mayo Graduate School on Techniques in Molecular Biology, Human Cytogenetics, and Immunology. ..