ROGER SENDER LASKEN

Summary

Affiliation: J. Craig Venter Institute
Country: USA

Publications

  1. ncbi Single-cell genomic sequencing using Multiple Displacement Amplification
    Roger S Lasken
    J Craig Venter Institute, 11149 N Torrey Pines Rd, Suite 200, La Jolla, CA 92037, USA
    Curr Opin Microbiol 10:510-6. 2007
  2. ncbi Genomic DNA amplification from a single bacterium
    Arumugham Raghunathan
    Molecular Staging, Inc, New Haven, Connecticut, USA
    Appl Environ Microbiol 71:3342-7. 2005
  3. ncbi Specific single-cell isolation and genomic amplification of uncultured microorganisms
    Thomas Kvist
    Environmental Microbiology and Biotechnology Group, BioCentrum Technical University of Denmark, Building 227, Lyngby, Denmark
    Appl Microbiol Biotechnol 74:926-35. 2007
  4. ncbi Mechanism of chimera formation during the Multiple Displacement Amplification reaction
    Roger S Lasken
    J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Biotechnol 7:19. 2007
  5. ncbi Genomic sequencing of single microbial cells from environmental samples
    Thomas Ishoey
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, United States
    Curr Opin Microbiol 11:198-204. 2008
  6. ncbi Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluid
    Sandeep Kathju
    Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
    BMC Res Notes 3:259. 2010
  7. ncbi Genomic DNA amplification by the multiple displacement amplification (MDA) method
    Roger S Lasken
    J Craig Venter Institute, La Jolla, CA 92121, USA
    Biochem Soc Trans 37:450-3. 2009
  8. ncbi Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens
    Roger S Lasken
    Molecular Staging Inc, 300 George Street, New Haven, CT 06511, USA
    Trends Biotechnol 21:531-5. 2003
  9. ncbi A full-coverage, high-resolution human chromosome 22 genomic microarray for clinical and research applications
    Patrick G Buckley
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden
    Hum Mol Genet 11:3221-9. 2002
  10. ncbi Something from (almost) nothing: the impact of multiple displacement amplification on microbial ecology
    Erik K Binga
    Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
    ISME J 2:233-41. 2008

Research Grants

  1. WHOLE GENOME AMPLIFICATION FOR RAPID LOW COST GENOTYPING
    ROGER LASKEN; Fiscal Year: 2005
  2. Single Cell Genomics of Human Microbial Flora
    ROGER SENDER LASKEN; Fiscal Year: 2010

Collaborators

  • Josh D Neufeld
  • Ramunas Stepanauskas
  • Marc Mussmann
  • A Fawad Faruqi
  • Seiyu Hosono
  • Sandeep Kathju
  • Wanmin Song
  • Xiaohong Wu
  • Yuefen Du
  • Jing Du
  • Michael Egholm
  • Zhenyu Sun
  • Frank B Dean
  • Erik K Binga
  • Thomas Ishoey
  • Traci Kiesling
  • Thomas Kvist
  • Arumugham Raghunathan
  • David L Barker
  • Carole J Bornarth
  • Mark Driscoll
  • Osama A Alsmadi
  • Stephen F Kingsmore
  • Patrick G Buckley
  • Paul Stoodley
  • Latha Satish
  • Garth D Ehrlich
  • J Christopher Post
  • Sandra Johnson
  • Tanja Woyke
  • Mark Novotny
  • Birgitte K Ahring
  • Evan Skowronski
  • Mark Danielsen
  • Kenneth Dretchen
  • Peter Westermann
  • Eugene A Davidson
  • Kendra Cox
  • Guy Grater
  • Jonathan Leshin
  • Shannon Hibbard
  • Harley R Ferguson
  • Jonathon H Pinter
  • Diane Giannola
  • Vladimir Makarov
  • Mark S T Hansen
  • William Ziehler
  • Orlando R Irsula
  • Martin Latterich
  • Richard Shen
  • Arnold Oliphant
  • Eric Lai
  • Irina Sleptsova
  • Joel P Brockman
  • Michele Wisniewski
  • Mark D Driscoll
  • PATRICIO ABARZUA
  • Patricia Bray-Ward
  • Ingegerd Fransson
  • Magdalena Benetkiewicz
  • Nils Mandahl
  • Elisabeth Blennow
  • Britt-Inger Johnsson
  • Charlotte Thyr
  • Kiran K Mantripragada
  • Anna Wedell
  • Ian Dunham
  • John E Collins
  • V Peter Collins
  • Martin Hergersberg
  • David M Beare
  • Carl E G Bruder
  • Magnus Rosenquist
  • Linhua Fang
  • Uwe Menzel
  • Qiuling Zong
  • Caroline Jarbo
  • Daniel Pinkel
  • Jan P Dumanski
  • Donna Albertson
  • Haider Ali
  • Koichi Ichimura
  • Boris C Bastian

Detail Information

Publications16

  1. ncbi Single-cell genomic sequencing using Multiple Displacement Amplification
    Roger S Lasken
    J Craig Venter Institute, 11149 N Torrey Pines Rd, Suite 200, La Jolla, CA 92037, USA
    Curr Opin Microbiol 10:510-6. 2007
    ..The genetically linked sequences from single cells are also a powerful tool to be used in guiding genomic assembly of shotgun sequences of multiple organisms from environmental DNA extracts (metagenomic sequences)...
  2. ncbi Genomic DNA amplification from a single bacterium
    Arumugham Raghunathan
    Molecular Staging, Inc, New Haven, Connecticut, USA
    Appl Environ Microbiol 71:3342-7. 2005
    ..A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms...
  3. ncbi Specific single-cell isolation and genomic amplification of uncultured microorganisms
    Thomas Kvist
    Environmental Microbiology and Biotechnology Group, BioCentrum Technical University of Denmark, Building 227, Lyngby, Denmark
    Appl Microbiol Biotechnol 74:926-35. 2007
    ..The system was validated using Methanothermobacter thermoautotrophicus as single-cell test organism, and the validation setup produced 100% sequence homology to the ten tested regions of the genome of this organism...
  4. ncbi Mechanism of chimera formation during the Multiple Displacement Amplification reaction
    Roger S Lasken
    J Craig Venter Institute, Rockville, MD 20850, USA
    BMC Biotechnol 7:19. 2007
    ..Determining the source of the DNA rearrangements would be an important step towards reducing or eliminating them...
  5. ncbi Genomic sequencing of single microbial cells from environmental samples
    Thomas Ishoey
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, United States
    Curr Opin Microbiol 11:198-204. 2008
    ..Methods are reviewed for preparation of bacterial fractions from environmental samples, single-cell isolation, DNA amplification by MDA, and DNA sequencing...
  6. ncbi Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluid
    Sandeep Kathju
    Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
    BMC Res Notes 3:259. 2010
    ..aureus DNA as well as on clinical samples known to contain S. aureus nucleic acids...
  7. ncbi Genomic DNA amplification by the multiple displacement amplification (MDA) method
    Roger S Lasken
    J Craig Venter Institute, La Jolla, CA 92121, USA
    Biochem Soc Trans 37:450-3. 2009
    ..Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed...
  8. ncbi Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens
    Roger S Lasken
    Molecular Staging Inc, 300 George Street, New Haven, CT 06511, USA
    Trends Biotechnol 21:531-5. 2003
  9. ncbi A full-coverage, high-resolution human chromosome 22 genomic microarray for clinical and research applications
    Patrick G Buckley
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden
    Hum Mol Genet 11:3221-9. 2002
    ..Furthermore, comprehensive epigenetic profiling of 22q-located genes and high-resolution analysis of replication timing across the entire chromosome can be studied using our array...
  10. ncbi Something from (almost) nothing: the impact of multiple displacement amplification on microbial ecology
    Erik K Binga
    Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
    ISME J 2:233-41. 2008
    ..Whole genome amplification via MDA has increased access to the genomic DNA of uncultivated microorganisms and low-biomass environments and represents a 'power tool' in the molecular toolbox of microbial ecologists...
  11. ncbi Unbiased whole-genome amplification directly from clinical samples
    Seiyu Hosono
    Molecular Staging, Inc, New Haven, Connecticut 06511, USA
    Genome Res 13:954-64. 2003
    ..The low amplification bias of MDA represents a dramatic technical improvement in the ability to amplify a whole genome compared with older, PCR-based methods...
  12. ncbi Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)
    Traci Kiesling
    Department of Biochemistry and Molecular Biology and Cellular Biology, Georgetown University School of Medicine, Washington, DC 20057, USA
    Nucleic Acids Res 35:e117. 2007
    ..This method should be applicable whenever there is a requirement to detect a specific DNA sequence. Other applications include SNP analysis and genotyping. The reaction is inherently simple to multiplex and is amenable to automation...
  13. ncbi Insights into the genome of large sulfur bacteria revealed by analysis of single filaments
    Marc Mussmann
    Max Planck Institute for Marine Microbiology, Bremen, Germany
    PLoS Biol 5:e230. 2007
    ..The first look into the genome of these filamentous sulfur-oxidizing bacteria substantially deepens the understanding of their evolution and their contribution to sulfur and nitrogen cycling in marine sediments...
  14. ncbi High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay
    Osama A Alsmadi
    Molecular Staging, Inc, 300 George Street, Suite 701, New Haven, CT 06511, USA
    BMC Genomics 4:21. 2003
    ..The low cost and scalability of ligation/ERCA genotyping makes it ideally suited for automated, high throughput methods...
  15. ncbi Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel
    David L Barker
    Illumina, Inc, San Diego, California 92121, USA
    Genome Res 14:901-7. 2004
    ..The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples...
  16. ncbi Comprehensive human genome amplification using multiple displacement amplification
    Frank B Dean
    Molecular Staging, Inc, 300 George Street, Suite 701, New Haven, CT 06511, USA
    Proc Natl Acad Sci U S A 99:5261-6. 2002
    ..MDA-based WGA is a simple and reliable method that could have significant implications for genetic studies, forensics, diagnostics, and long-term sample storage...

Research Grants4

  1. WHOLE GENOME AMPLIFICATION FOR RAPID LOW COST GENOTYPING
    ROGER LASKEN; Fiscal Year: 2005
    ..C) Individual lymphocytes will be used as a model system to prove feasibility of doing preimplantation genetic diagnosis from single blastomeres. ..
  2. Single Cell Genomics of Human Microbial Flora
    ROGER SENDER LASKEN; Fiscal Year: 2010
    ....