Research Topics
| Uma LakshmipathySummaryAffiliation: Invitrogen Corporation Country: USA Publications
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Detail Information
Publications
Concise review: MicroRNA expression in multipotent mesenchymal stromal cellsUma Lakshmipathy
Stem Cells and Regenerative Medicine, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008, USA
Stem Cells 26:356-63. 2008..In this review, we describe the importance of microRNAs in stem cells, focusing on our current understanding of microRNAs in MSC and their derivatives...- MicroRNA expression pattern of undifferentiated and differentiated human embryonic stem cellsUma Lakshmipathy
Invitrogen Corporation, Carlsbad, CA 92008, USA
Stem Cells Dev 16:1003-16. 2007.... - hESC engineering by integrase-mediated chromosomal targetingYing Liu
Invitrogen Corporation, Carlsbad, CA, USA
Methods Mol Biol 584:229-68. 2010.... - BacMam-mediated gene delivery into multipotent mesenchymal stromal cellsMichael O'Grady
Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA
Methods Mol Biol 698:485-504. 2011..In this chapter, we describe methods of generating high titer stocks of BacMam for transducing MSC and their derivatives... - Creation of engineered human embryonic stem cell lines using phiC31 integraseBhaskar Thyagarajan
Invitrogen Corporation, Carlsbad, California 92008, USA
Stem Cells 26:119-26. 2008..Our results demonstrate the utility of phiC31 integrase to target pseudo-attP sites in hESC and show that integrase-mediated site-specific integration can efficiently create stably expressing engineered human embryonic stem cell clones... - A simplified culture and polymerase chain reaction identification assay for quality control performance testing of stem cell media productsShayne Boucher
Life Technologies, Primary and Stem Cell Systems, Frederick, Maryland 21704, USA
Cytotherapy 11:761-7, 767.e1-2. 2009.... - Chromatin insulator elements block transgene silencing in engineered human embryonic stem cell lines at a defined chromosome 13 locusChad C MacArthur
Primary and Stem Cell Systems, Life Technologies Corporation, Carlsbad, California, USA
Stem Cells Dev 21:191-205. 2012..This is a valuable tool for lineage development study, gain- and loss-of-function experiments, and human disease modeling using hESCs... - Generation of platform human embryonic stem cell lines that allow efficient targeting at a predetermined genomic locationYing Liu
Primary and Stem Cell Systems, Life Technologies Corporation, Carlsbad, California 92008, USA
Stem Cells Dev 18:1459-72. 2009..The system described here represents a method to rapidly assemble complex plasmid-based assay systems, controllably insert them into the hESC genome, and have them actively express in undifferentiated as well as in differentiated cells... - A single EBV-based vector for stable episomal maintenance and expression of GFP in human embryonic stem cellsBhaskar Thyagarajan
Primary and Stem Cell Systems, Life Technologies, 5781 Van Allen Way, Carlsbad, CA 92008, USA
Regen Med 4:239-50. 2009..In this study, we describe a vector that is maintained episomally in hESCs... - Efficient transfection of embryonic and adult stem cellsUma Lakshmipathy
Stem Cell Institute, Hematology, Oncology and Transplantation Division, University of Minnesota, Minneapolis 55455, USA
Stem Cells 22:531-43. 2004..The high levels of transfection achieved by nucleofection will enable its use as a rapid screening tool to evaluate the effect of ectopically expressed transcription factors on tissue-specific differentiation of stem cells... - Gene transfer via nucleofection into adult and embryonic stem cellsUma Lakshmipathy
Stem Cell Institute, University of Minnesota, Minneapolis, USA
Methods Mol Biol 407:115-26. 2007..Various types of adult and embryonic stem cells can be successfully transfected using this method, as described in this chapter... - PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and oFelicia Ng
Genome and Gene Expression Analysis Group, Bioinformatics Institute, Agency for Science Technology and Research A STAR, Singapore
Blood 112:295-307. 2008..Thus, this study illustrates it is possible to predict signaling pathways active in cellular differentiation and growth using microarray data and experimentally verify these predictions... - Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell researchRichard Josephson
GlobalStem, Inc, 6 Taft Court, Rockville, Maryland 20850, USA
Stem Cells 25:437-46. 2007..On the basis of global gene and microRNA expression, 2102Ep is somewhat less similar to hESC than the alternatives; however, 2102Ep expresses more hESC-associated microRNAs than NTERA-2 does, and fewer markers of differentiated fates... - Stem cell plasticityUma Lakshmipathy
Stem Cell Institute, Hematology, Oncology and Transplantation Division, Department of Medicine, University of Minnesota, 420 Delaware Street, Minneapolis, MN 55455, USA
Blood Rev 19:29-38. 2005.... - Micro RNA profiling: an easy and rapid method to screen and characterize stem cell populationsUma Lakshmipathy
Stem Cell Institute, University of Minnesota, Minneapolis, USA
Methods Mol Biol 407:97-114. 2007..In this chapter, we describe in detail the method to carry out miRNA array analysis in human embryonal carcinoma cells and confirm the array results using QPCR...