Research Topics
| Daniel B KearnsSummaryAffiliation: Indiana University Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motilityDaniel B Kearns
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Mol Microbiol 52:357-69. 2004..These findings suggest that the swrA insertion and its reversion take place by slipped-strand mispairing during DNA replication and that swarming motility is subject to phase variation...- Multi-species integrative biclusteringPeter Waltman
Computer Science Department, Warren Weaver Hall Room 305, 251 Mercer Street, New York, NY 10012, USA
Genome Biol 11:R96. 2010.... - A defined medium to investigate sliding motility in a Bacillus subtilis flagella-less mutantRay Fall
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
BMC Microbiol 6:31. 2006..Here we have used a flagella-less hag null mutant to examine and confirm sliding motility... - Division of labour during Bacillus subtilis biofilm formationDaniel B Kearns
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Mol Microbiol 67:229-31. 2008..The population heterogeneity that they observe is another significant example in a growing list of phenotypes that arise owing to bistable gene expression... - Microbiology. Bright insight into bacterial glidingDaniel B Kearns
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Science 315:773-4. 2007 - A field guide to bacterial swarming motilityDaniel B Kearns
Indiana University, Bloomington, Indiana 47405, USA
Nat Rev Microbiol 8:634-44. 2010.... - RemA (YlzA) and RemB (YaaB) regulate extracellular matrix operon expression and biofilm formation in Bacillus subtilisJared T Winkelman
Department of Biology, Indiana University, Bloomington, IN 47405, USA
J Bacteriol 191:3981-91. 2009..Both proteins are required for the activation of the matrix biosynthesis operons and appear to act in parallel to SinR and two other known biofilm regulators, AbrB and DegU... - The EpsE flagellar clutch is bifunctional and synergizes with EPS biosynthesis to promote Bacillus subtilis biofilm formationSarah B Guttenplan
Department of Biology, Indiana University, Bloomington, Indiana, United States of America
PLoS Genet 6:e1001243. 2010..Thus, the transition from motility to biofilm formation may be governed by a single bifunctional enzyme... - Gene position in a long operon governs motility development in Bacillus subtilisLoralyn M Cozy
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Mol Microbiol 76:273-85. 2010..subtilis are a form of development because mosaics of stable and differentiated epigenotypes were evident when the normally dispersed bacteria were forced to grow in one dimension... - DegU-phosphate activates expression of the anti-sigma factor FlgM in Bacillus subtilisYi Huang Hsueh
Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47405, USA
Mol Microbiol 81:1092-108. 2011..Regulation of FlgM is poorly understood outside of Salmonella, and differential control of FlgM expression may be a common means of coupling flagellin expression to flagellar assembly... - A molecular clutch disables flagella in the Bacillus subtilis biofilmKris M Blair
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Science 320:1636-8. 2008..The clutch is a simple, rapid, and potentially reversible form of motility control... - Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneityRui Chen
Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47405, USA
J Bacteriol 191:5775-84. 2009..We conclude that heterogeneous activation of sigma(D)-dependent gene expression is sufficient to explain both the morphological heterogeneity and functional heterogeneity present in vegetative B. subtilis populations... - Swarming motility and the control of master regulators of flagellar biosynthesisJoyce E Patrick
Indiana University, Department of Biology, 1001 East Third Street, Bloomington, IN 47405, USA
Mol Microbiol 83:14-23. 2012..We hypothesize that environmental control of the master regulators is important to the swarming phenotype perhaps at the level of controlling flagellar number... - CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilisSampriti Mukherjee
Department of Biology, Indiana University, Bloomington, IN 47408, USA
Mol Microbiol 82:447-61. 2011..Finally, phylogenetic analysis suggests that CsrA, a highly pleiotropic virulence regulator in many bacterial pathogens, had an ancestral role in flagellar assembly and evolved to co-regulate various cellular processes with motility... - MinJ (YvjD) is a topological determinant of cell division in Bacillus subtilisJoyce E Patrick
Department of Biology, Indiana University, Bloomington, IN 47405, USA
Mol Microbiol 70:1166-79. 2008..MinJ itself localized to cell poles in a manner that was dependent on DivIVA. MinJ is conserved in other low G+C Gram-positive bacteria and may be an important component of cell division site selection in these organisms... - SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of ?(D) in Bacillus subtilisLoralyn M Cozy
Indiana University, Department of Biology, Bloomington, IN 47405, USA
Mol Microbiol 83:1210-28. 2012..Thus, flagellin gene expression demonstrated hypersensitivity and hysteresis and we conclude that ?(d) -dependent gene expression is bistable... - Laboratory strains of Bacillus subtilis do not exhibit swarming motilityJoyce E Patrick
Indiana University, Department of Biology, 1001 East Third Street, Bloomington, IN 47405, USA
J Bacteriol 191:7129-33. 2009..We attribute discrepancies in the literature to a lack of reproducible standard experimental conditions, selection for spontaneous swarming suppressors, inadvertent genetic linkage to swarming mutations, and auxotrophy... - Modified mariner transposons for random inducible-expression insertions and transcriptional reporter fusion insertions in Bacillus subtilisEric R Pozsgai
Department of Biology, Indiana University, Bloomington, Indiana, USA
Appl Environ Microbiol 78:778-85. 2012..subtilis and should be applicable to any mariner-compatible host organism, provided that in vitro mutagenesis or an in vivo species-specific delivery vector is employed... - A master regulator for biofilm formation by Bacillus subtilisDaniel B Kearns
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Mol Microbiol 55:739-49. 2005.... - Genetic requirements for potassium ion-dependent colony spreading in Bacillus subtilisRebecca F Kinsinger
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
J Bacteriol 187:8462-9. 2005..subtilis to colonize surfaces by spreading is highly dependent on balanced nucleotide biosynthesis and nutrient assimilation, which require sufficient K(+) ions, as well as growth conditions that promote sliding motility... - Cell population heterogeneity during growth of Bacillus subtilisDaniel B Kearns
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Genes Dev 19:3083-94. 2005..Cell population heterogeneity could enable B. subtilis to exploit its present location through the production of sessile cells as well as to explore new environmental niches through the generation of nomadic cells... - Targets of the master regulator of biofilm formation in Bacillus subtilisFrances Chu
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Mol Microbiol 59:1216-28. 2006..These findings reinforce the view that SinR is a master regulator for biofilm formation and further suggest that a principal biological function of SinR is to govern the assembly of complex multicellular communities... - A major protein component of the Bacillus subtilis biofilm matrixSteven S Branda
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
Mol Microbiol 59:1229-38. 2006..Having defined the major components of the biofilm matrix and the control of their synthesis by the global regulator SinR, we present a working model for how B. subtilis switches between nomadic and sedentary lifestyles... - A novel regulatory protein governing biofilm formation in Bacillus subtilisFrances Chu
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Mol Microbiol 68:1117-27. 2008..These findings reveal that matrix production is governed by an intricate network involving the interplay of negatively and positively acting regulatory proteins... - An extracellular matrix-associated zinc metalloprotease is required for dilauroyl phosphatidylethanolamine chemotactic excitation in Myxococcus xanthusDaniel B Kearns
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
J Bacteriol 184:1678-84. 2002..Chemotactic excitation of the fibA mutant was restored by incubation with purified wild-type fibrils. The results suggest that this metalloprotease is involved in sensory transduction... - Swarming motility in undomesticated Bacillus subtilisDaniel B Kearns
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
Mol Microbiol 49:581-90. 2003..We conclude that robust swarming is a feature of undomesticated B. subtilis and that this behaviour has been lost or attenuated in laboratory strains through the accumulation of multiple genetic defects...
Research Grants
- The mechanism of bistable motility gene expression in Bacillus subtilis.Daniel B Kearns; Fiscal Year: 2010..PUBLIC HEALTH RELEVANCE: We will study flagellar-mediated motility in Bacillus subtilis and we will determine the kinetics, mechanism, and regulation of a bistable epigenetic switching controlling flagellin and autolysin expression. ..