Roman Dziarski

Summary

Affiliation: Indiana University School of Medicine
Country: USA

Publications

  1. ncbi Staphylococcus aureus peptidoglycan is a toll-like receptor 2 activator: a reevaluation
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA
    Infect Immun 73:5212-6. 2005
  2. ncbi Recognition of bacterial peptidoglycan by the innate immune system
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, Indiana 46408, USA
    Cell Mol Life Sci 60:1793-804. 2003
  3. ncbi Peptidoglycan recognition in innate immunity
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Endotoxin Res 11:304-10. 2005
  4. ncbi Defect in neutrophil killing and increased susceptibility to infection with nonpathogenic gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)-deficient mice
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA
    Blood 102:689-97. 2003
  5. ncbi Mammalian PGRPs: novel antibacterial proteins
    Roman Dziarski
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Cell Microbiol 8:1059-69. 2006
  6. ncbi Role of MD-2 in TLR2- and TLR4-mediated recognition of Gram-negative and Gram-positive bacteria and activation of chemokine genes
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana, USA
    J Endotoxin Res 6:401-5. 2000
  7. ncbi The peptidoglycan recognition proteins (PGRPs)
    Roman Dziarski
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Genome Biol 7:232. 2006
  8. ncbi Peptidoglycan recognition proteins (PGRPs)
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA
    Mol Immunol 40:877-86. 2004
  9. ncbi MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, IN 46408, USA
    J Immunol 166:1938-44. 2001
  10. ncbi Review: Mammalian peptidoglycan recognition proteins (PGRPs) in innate immunity
    Roman Dziarski
    Indiana University School of Medicine Northwest, Gary, USA
    Innate Immun 16:168-74. 2010

Research Grants

  1. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2006
  2. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2007
  3. Antibacterial peptidoglycan recognition proteins
    Roman Dziarski; Fiscal Year: 2007
  4. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2009
  5. Mechanism of antibacterial activity of peptidoglycan recognition proteins
    Roman Dziarski; Fiscal Year: 2009
  6. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2010
  7. Mechanism of antibacterial activity of peptidoglycan recognition proteins
    Roman Dziarski; Fiscal Year: 2010
  8. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 1993
  9. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2001
  10. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2003

Collaborators

Detail Information

Publications35

  1. ncbi Staphylococcus aureus peptidoglycan is a toll-like receptor 2 activator: a reevaluation
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA
    Infect Immun 73:5212-6. 2005
    ..The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator...
  2. ncbi Recognition of bacterial peptidoglycan by the innate immune system
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, Indiana 46408, USA
    Cell Mol Life Sci 60:1793-804. 2003
    ..These molecules induce host responses to microorganisms or have direct antimicrobial effects...
  3. ncbi Peptidoglycan recognition in innate immunity
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Endotoxin Res 11:304-10. 2005
    ..These molecules induce host responses to micro-organisms, degrade PGN, or have direct antimicrobial effects...
  4. ncbi Defect in neutrophil killing and increased susceptibility to infection with nonpathogenic gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)-deficient mice
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA
    Blood 102:689-97. 2003
    ..Therefore, mammalian PGRP-S functions in intracellular killing of bacteria. Thus, only bacterial recognition by PGRP-S, but not its effector function, is conserved from insects to mammals...
  5. ncbi Mammalian PGRPs: novel antibacterial proteins
    Roman Dziarski
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Cell Microbiol 8:1059-69. 2006
    ..Direct bactericidal activity of these PGRPs either evolved in vertebrates or mammals, or it is yet to be discovered in insects...
  6. ncbi Role of MD-2 in TLR2- and TLR4-mediated recognition of Gram-negative and Gram-positive bacteria and activation of chemokine genes
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana, USA
    J Endotoxin Res 6:401-5. 2000
    ..Therefore, TLR2 is a functional receptor for both Gram-positive and Gram-negative bacteria and it induces activation of IL-8...
  7. ncbi The peptidoglycan recognition proteins (PGRPs)
    Roman Dziarski
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Genome Biol 7:232. 2006
    ..Direct bactericidal activity of these PGRPs either evolved in the vertebrate (or mammalian) lineage or is yet to be discovered in insects...
  8. ncbi Peptidoglycan recognition proteins (PGRPs)
    Roman Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA
    Mol Immunol 40:877-86. 2004
    ..One function, amidase activity, is shared by some insect and mammalian PGRPs, whereas antibacterial activity of some mammalian PGRPs is unique for mammals...
  9. ncbi MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, IN 46408, USA
    J Immunol 166:1938-44. 2001
    ....
  10. ncbi Review: Mammalian peptidoglycan recognition proteins (PGRPs) in innate immunity
    Roman Dziarski
    Indiana University School of Medicine Northwest, Gary, USA
    Innate Immun 16:168-74. 2010
    ..They modulate local inflammatory responses in tissues (such as arthritic joints) and there is evidence for association of PGRPs with inflammatory diseases, such as psoriasis...
  11. ncbi PGLYRP-2 and Nod2 are both required for peptidoglycan-induced arthritis and local inflammation
    Sukumar Saha
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Cell Host Microbe 5:137-50. 2009
    ..Our results demonstrate that PGRPs, Nod2, and TLR4, representing three different types of pattern-recognition molecules, play interdependent in vivo roles in local inflammation...
  12. ncbi Peptidoglycan recognition proteins protect mice from experimental colitis by promoting normal gut flora and preventing induction of interferon-gamma
    Sukumar Saha
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Cell Host Microbe 8:147-62. 2010
    ....
  13. ncbi Differential effects of peptidoglycan recognition proteins on experimental atopic and contact dermatitis mediated by Treg and Th17 cells
    Shin Yong Park
    Indiana University School of Medicine Northwest, Gary, Indiana, United States of America
    PLoS ONE 6:e24961. 2011
    ....
  14. ncbi Human peptidoglycan recognition proteins require zinc to kill both gram-positive and gram-negative bacteria and are synergistic with antibacterial peptides
    Minhui Wang
    Indiana University School of Medicine, Northwest Campus, 3400 Broadway, Gary, IN 46408, USA
    J Immunol 178:3116-25. 2007
    ....
  15. ncbi Zebrafish peptidoglycan recognition proteins are bactericidal amidases essential for defense against bacterial infections
    Xinna Li
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    Immunity 27:518-29. 2007
    ..These data demonstrate an absolute requirement for innate immunity in defense against infections in fish embryos and for a PGRP protein for survival in vertebrates...
  16. ncbi Peptidoglycan recognition proteins kill bacteria by activating protein-sensing two-component systems
    Des Raj Kashyap
    Indiana University School of Medicine Northwest, Gary, Indiana, USA
    Nat Med 17:676-83. 2011
    ..Thus, we reveal a previously unknown mechanism by which innate immunity proteins that bind the cell wall or outer membrane exploit the bacterial stress defense response to kill bacteria...
  17. ncbi Peptidoglycan Recognition Protein Pglyrp2 Protects Mice from Psoriasis-like Skin Inflammation by Promoting Regulatory T Cells and Limiting Th17 Responses
    Shin Yong Park
    Indiana University School of Medicine Northwest, Gary, IN 46408
    J Immunol 187:5813-23. 2011
    ..Thus, in wild type mice, Pglyrp2 limits overactivation of Th17 cells by promoting accumulation of regulatory T cells at the site of inflammation, which protects the skin from the exaggerated inflammatory response...
  18. ncbi Identification of serum N-acetylmuramoyl-l-alanine amidase as liver peptidoglycan recognition protein 2
    Yinong Zhang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Biochim Biophys Acta 1752:34-46. 2005
    ..Serum NAMLAA had a C398-C404 disulfide, partial phosphorylation of S218, and deamidation of N253 and N301. These results indicate that serum NAMLAA and liver PGLYRP2 are the same protein encoded by the pglyrp2 gene...
  19. ncbi Peptidoglycan recognition proteins are a new class of human bactericidal proteins
    Xiaofeng Lu
    Indiana University School of Medicine Northwest, Gary, IN 46408, USA
    J Biol Chem 281:5895-907. 2006
    ....
  20. ncbi Binding of bacterial peptidoglycan to CD14
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Biol Chem 273:8680-90. 1998
    ....
  21. ncbi Micrococci and peptidoglycan activate TLR2-->MyD88-->IRAK-->TRAF-->NIK-->IKK-->NF-kappaB signal transduction pathway that induces transcription of interleukin-8
    Q Wang
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    Infect Immun 69:2270-6. 2001
    ..These data indicate that micrococci and PGN induce TLR2-dependent activation of the gene for IL-8 and that this activation requires MyD88, IRAK, NIK, IKK, and NF-kappaB and may also utilize TRAF6 and, to a lesser extent, TRAF2...
  22. ncbi Peptidoglycan recognition protein 2 (N-acetylmuramoyl-L-Ala amidase) is induced in keratinocytes by bacteria through the p38 kinase pathway
    Haitao Wang
    Indiana University School of Medicine Northwest, 3400 Broadway, Gary, IN 46408, USA
    Infect Immun 73:7216-25. 2005
    ..PGLYRP2 may function in the skin and the eyes as an inducible scavenger of proinflammatory peptidoglycan...
  23. ncbi Soluble CD14 enhances membrane CD14-mediated responses to peptidoglycan: structural requirements differ from those for responses to lipopolysaccharide
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    Infect Immun 68:5254-60. 2000
    ..Thus, there are different structural requirements of sCD14 for binding to PGN and to LPS and for the enhancement of PGN- and LPS-induced cell activation...
  24. ncbi Human peptidoglycan recognition protein-L is an N-acetylmuramoyl-L-alanine amidase
    Zheng Ming Wang
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Biol Chem 278:49044-52. 2003
    ..In conclusion, human PGRP-L is an N-acetylmuramoyl-l-alanine amidase and this function is conserved in prokaryotes, insects, and mammals...
  25. ncbi Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1
    D Gupta
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Biol Chem 274:14012-20. 1999
    ..This is the first study to demonstrate activation of CREB/ATF and AP-1 transcription factors by PGN or by any other component of Gram-positive bacteria...
  26. ncbi Chemokines are the main proinflammatory mediators in human monocytes activated by Staphylococcus aureus, peptidoglycan, and endotoxin
    Z M Wang
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Biol Chem 275:20260-7. 2000
    ..These results establish chemokines as the main mediators induced by both Gram-positive and Gram-negative bacteria and are consistent with the highly inflammatory nature of bacterial infections...
  27. ncbi CD14 is a cell-activating receptor for bacterial peptidoglycan
    D Gupta
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Biol Chem 271:23310-6. 1996
    ....
  28. ncbi Bacterial peptidoglycan binds to tubulin
    R Dziarski
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, 46408, USA
    Biochim Biophys Acta 1524:17-26. 2001
    ..These results indicate that tubulin is a major PGN-binding protein and that PGN modulates tubulin polymerization...
  29. ncbi Peptidoglycan induces transcription and secretion of TNF-alpha and activation of lyn, extracellular signal-regulated kinase, and rsk signal transduction proteins in mouse macrophages
    D Gupta
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary 46408, USA
    J Immunol 155:2620-30. 1995
    ....
  30. ncbi Peptidoglycan recognition proteins: a novel family of four human innate immunity pattern recognition molecules
    C Liu
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana 46408, USA
    J Biol Chem 276:34686-94. 2001
    ..All four human PGRPs bind peptidoglycan and Gram-positive bacteria. Thus, these PGRPs may play a role in recognition of bacteria in these organs...
  31. ncbi Bacterial peptidoglycan-induced tnf-alpha transcription is mediated through the transcription factors Egr-1, Elk-1, and NF-kappaB
    Z Xu
    Northwest Center for Medical Education, Indiana University School of Medicine, Gary, IN 46408, USA
    J Immunol 167:6975-82. 2001
    ....
  32. ncbi Peptidoglycan recognition protein-S (PGRP-S) is upregulated by NF-kappaB
    Ming Fei Lang
    Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany
    Neurosci Lett 430:138-41. 2008
    ..However, there was no difference in the infarct size in PGRP-S-deficient mice compared to controls. In summary, the data show that PGRP-S is induced in cerebral ischemia by RelA, but its role in ischemia is unclear...
  33. ncbi A subset of Toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells
    Sandip K Datta
    Department of Medicine and The Sam and Rose Stein Institute for Research on Aging, University of California, La Jolla, CA 92093, USA
    J Immunol 170:4102-10. 2003
    ..In contrast to presentation of Ag to CD4(+) T cells by immature DCs, TLR-induced cross-presentation is mediated by mature DCs, is independent of endosomal acidification, and relies on cytosolic Ag processing machinery...
  34. ncbi Peptidoglycan recognition proteins: pleiotropic sensors and effectors of antimicrobial defences
    Julien Royet
    Institut de Biologie du Developpement de Marseille Luminy, UMR 6216 CNRS, Université de la Méditérannée Aix Marseille II, Marseille, France
    Nat Rev Microbiol 5:264-77. 2007
    ..By contrast, mammalian PGRPs are directly bactericidal. Here, we review these contrasting modes of action...
  35. ncbi Deadly plague versus mild-mannered TLR4
    Roman Dziarski
    Nat Immunol 7:1017-9. 2006

Research Grants22

  1. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2006
    ..This study may discover a new mechanism of innate immunity and a new immunodeficiency, and may provide tools for developing new preventive and therapeutic measures against infections with Gram-positive bacteria. ..
  2. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2007
    ..This study may discover a new mechanism of innate immunity and a new immunodeficiency, and may provide tools for developing new preventive and therapeutic measures against infections with Gram-positive bacteria. ..
  3. Antibacterial peptidoglycan recognition proteins
    Roman Dziarski; Fiscal Year: 2007
    ..abstract_text> ..
  4. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2009
    ..This project may discover new mechanisms of resistance to infections, new susceptibility genes for inflammatory bowel disease, and new mechanisms of regulation of bacterially-induced arthritis, encephalitis, and antibody responses. ..
  5. Mechanism of antibacterial activity of peptidoglycan recognition proteins
    Roman Dziarski; Fiscal Year: 2009
    ..New antibacterial agents are needed because of the increasing resistance of bacteria to commonly used antibiotics. ..
  6. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2010
    ..This project may discover new mechanisms of resistance to infections, new susceptibility genes for inflammatory bowel disease, and new mechanisms of regulation of bacterially-induced arthritis, encephalitis, and antibody responses. ..
  7. Mechanism of antibacterial activity of peptidoglycan recognition proteins
    Roman Dziarski; Fiscal Year: 2010
    ..New antibacterial agents are needed because of the increasing resistance of bacteria to commonly used antibiotics. ..
  8. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 1993
    ....
  9. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2001
    ....
  10. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2003
    ..This study may discover a new mechanism of innate immunity and a new immunodeficiency, and may provide tools for developing new preventive and therapeutic measures against infections with Gram-positive bacteria. ..
  11. MECHANISM OF PATHOGENIC ACTIVITY OF PEPTIDOGLYCAN
    Roman Dziarski; Fiscal Year: 2011
    ..This project may discover new mechanisms of resistance to infections, new susceptibility genes for inflammatory bowel disease, and new mechanisms of regulation of bacterially-induced arthritis, encephalitis, and antibody responses. ..