Research Topics
| George M WhitesidesSummaryAffiliation: Harvard University Country: USA Publications
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Detail Information
Publications
Partitioning microfluidic channels with hydrogel to construct tunable 3-D cellular microenvironmentsAmy P Wong
Harvard Biophysics Program, Harvard Medical School, Boston, MA 02115, USA
Biomaterials 29:1853-61. 2008..This pair of cell lines represents a simple model system for intercellular communication: the LADMAC cells produce colony-stimulating factor 1 (CSF-1), which is required by the BAC cells for survival...- The origins and the future of microfluidicsGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138
Nature 442:368-73. 2006..The solutions to these problems will require imagination and ingenuity... - Fabrication of arrays of metal and metal oxide nanotubes by shadow evaporationMichael D Dickey
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
ACS Nano 2:800-8. 2008.... - Patterning the tips of optical fibers with metallic nanostructures using nanoskivingDarren J Lipomi
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nano Lett 11:632-6. 2011..Examples of structures transferred include gold crescents, rings, high-aspect-ratio concentric cylinders, and gratings of parallel nanowires... - Fabrication of large-area patterned nanostructures for optical applications by nanoskivingQiaobing Xu
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts, 02138, USA
Nano Lett 7:2800-5. 2007.... - Heterogeneous films of ionotropic hydrogels fabricated from delivery templates of patterned paperPaul J Bracher
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
ACS Appl Mater Interfaces 1:1807-12. 2009..The heterogeneous hydrogel films can be used to culture bacteria in various 2-D designs. The pattern of toxic and nontoxic ions used to cross-link the polymer determines the pattern of viable colonies of Escherichia coli within the film... - Thread as a matrix for biomedical assaysMeital Reches
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
ACS Appl Mater Interfaces 2:1722-8. 2010.... - Water-soluble sacrificial layers for surface micromachiningVincent Linder
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
Small 1:730-6. 2005.... - Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paperAudrey K Ellerbee
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Anal Chem 81:8447-52. 2009..This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries... - Thermodynamic parameters for the association of fluorinated benzenesulfonamides with bovine carbonic anhydrase IIVijay M Krishnamurthy
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Chem Asian J 2:94-105. 2007..Calorimetry revealed that all of the ligands studied bind in a 1:1 stoichiometry with BCA; this result was confirmed by 19F NMR spectroscopy and X-ray crystallography (for complexes with human carbonic anhydrase II)... - Increasing the net charge and decreasing the hydrophobicity of bovine carbonic anhydrase decreases the rate of denaturation with sodium dodecyl sulfateKatherine L Gudiksen
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Biophys J 91:298-310. 2006..At the high numbers of acylations, hydrophobic interactions cause the hexanoyl-modified BCA to denature nearly three orders of magnitude more rapidly than the acetyl-modified BCA... - Effects of surface charge on denaturation of bovine carbonic anhydraseIrina Gitlin
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Chembiochem 7:1241-50. 2006.... - Flowing lattices of bubbles as tunable, self-assembled diffraction gratingsMichinao Hashimoto
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA, USA
Small 2:1292-8. 2006..For our devices, we achieved tunable ranges in pitch from 12 to 51 microm, corresponding to first-order diffraction angles from 3.2 degrees to 0.7 degrees for light with a wavelength of 632 nm... - Pathway for unfolding of ubiquitin in sodium dodecyl sulfate, studied by capillary electrophoresisGregory F Schneider
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 130:17384-93. 2008..This study sheds light on the formation of the enigmatic protein-SDS complexes formed during SDS polyacrylamide gel electrophoresis and brings a new tool to the study of proteins and detergents... - Nanoskiving: a new method to produce arrays of nanostructuresQiaobing Xu
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, Massachusetts 02138, USA
Acc Chem Res 41:1566-77. 2008..This Account discusses a new fabrication method (nanoskiving) that produces arrays of metal nanostructures. The defining process in nanoskiving is cutting slabs from a polymeric matrix containing embedded, more extended metal structures... - Controlling the kinetics of contact electrification with patterned surfacesSamuel W Thomas
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 131:8746-7. 2009..More generally, these results show that our enhanced understanding of the ion-transfer mechanism of contact electrification enables the rational design of chemically tailored surfaces for functional electrets... - Peracetylated bovine carbonic anhydrase (BCA-Ac18) is kinetically more stable than native BCA to sodium dodecyl sulfateIrina Gitlin
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Phys Chem B 110:2372-7. 2006..Because it is experimentally impractical to prove equilibrium, it is not possible to establish whether there is a difference in the thermodynamics of unfolding/refolding between BCA and BCA-Ac18... - Using ion channel-forming peptides to quantify protein-ligand interactionsMichael Mayer
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 130:1453-65. 2008.... - Density-based diamagnetic separation: devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutionsAdam Winkleman
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Anal Chem 79:6542-50. 2007..This device requires no power, has no moving parts, and may be suitable for use in resource-poor environments... - Cofabrication: a strategy for building multicomponent microsystemsAdam C Siegel
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Acc Chem Res 43:518-28. 2010.... - Denaturation of proteins by SDS and tetraalkylammonium dodecyl sulfatesAndrew Lee
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts, USA
Langmuir 27:11560-74. 2011..These results suggest that the variation in the behavior of NR(4)(+)DS(-) with changes in R may be exploited in methods used to analyze and separate mixtures of proteins... - Programmable diagnostic devices made from paper and tapeAndres W Martinez
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Lab Chip 10:2499-504. 2010..They are the conceptual equivalent of field-programmable gate arrays (FPGAs) widely used in electronics... - Fluoroalkyl and alkyl chains have similar hydrophobicities in binding to the "hydrophobic wall" of carbonic anhydraseJasmin Mecinovic
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States
J Am Chem Soc 133:14017-26. 2011.... - Millimeter-scale contact printing of aqueous solutions using a stamp made out of paper and tapeChao Min Cheng
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Lab Chip 10:3201-5. 2010..By patterning the paper to which the transfer occurs using wax printing or an equivalent technique, accuracy increases substantially... - Integrated fabrication and magnetic positioning of metallic and polymeric nanowires embedded in thin epoxy slabsDarren J Lipomi
Harvard University, Department of Chemistry and Chemical Biology, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
ACS Nano 3:3315-25. 2009..Single-crystalline Au nanowires can be placed on glass wool fibers or on microfabricated polymeric waveguides, with which the nanowire can be addressed optically... - A general method for patterning gradients of biomolecules on surfaces using microfluidic networksXingyu Jiang
Department of Chemistry and Chemical Biology, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Anal Chem 77:2338-47. 2005..In this procedure, the relative amount of each protein, at saturation on the surface, depends only on its concentration.)... - Fabrication of thin, metallic films along the sidewalls of a topographically patterned stamp and their application in charge printingTingbing Cao
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Small 1:1191-5. 2005 - Using covalent dimers of human carbonic anhydrase II to model bivalency in immunoglobulinsEric T Mack
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 133:11701-15. 2011..These observations indicate that the avidities of these bivalent proteins, and by inference the avidities of structurally similar bivalent proteins such as IgG, are unexpectedly insensitive to the structure of the linker connecting them... - Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand bindingVijay M Krishnamurthy
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Chem Rev 108:946-1051. 2008 - A technique to transfer metallic nanoscale patterns to small and non-planar surfacesElizabeth J Smythe
School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, Massachusetts 02138, USA
ACS Nano 3:59-65. 2009..A distinguishing feature of this technique is the use of a thin, sacrificial film to strip and transfer metallic nanopatterns and its ability to directly transfer metallic structures produced by conventional lithography... - A non-chromatographic method for the purification of a bivalently active monoclonal IgG antibody from biological fluidsBasar Bilgicer
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 131:9361-7. 2009..It has the disadvantage that the structure of the hapten must be compatible with the synthesis of bi- and/or trivalent analogues... - Dependence of effective molarity on linker length for an intramolecular protein-ligand systemVijay M Krishnamurthy
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 129:1312-20. 2007.... - Multigait soft robotRobert F Shepherd
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 108:20400-3. 2011..A combination of crawling and undulation gaits allowed this robot to navigate a difficult obstacle. This demonstration illustrates an advantage of soft robotics: They are systems in which simple types of actuation produce complex motion... - Core-shell and segmented polymer-metal composite nanostructuresMichal Lahav
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nano Lett 6:2166-71. 2006..In the segmented structures, a self-assembled monolayer (SAM) of thioaniline nucleated the growth of PANI on top of metal nanorods and acted as an adhesion layer between the metal and PANI components... - Fabrication of high-aspect-ratio metallic nanostructures using nanoskivingQiaobing Xu
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Nano Lett 6:2163-5. 2006..The dimensions of the metal structures are determined by the thickness of the metal film and the thickness of the epoxy sections. The shape of the resulting nanostructure is defined by the cross section of the original template... - Eliminating positively charged lysine epsilon-NH3+ groups on the surface of carbonic anhydrase has no significant influence on its folding from sodium dodecyl sulfateKatherine L Gudiksen
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
J Am Chem Soc 127:4707-14. 2005..This study reinforces the idea that charged residues on the surface of BCA do not guide protein folding and raises the broader question of why proteins have charged residues on their surface, outside of the region of the active site... - Formation of bubbles and droplets in parallel, coupled flow-focusing geometriesMichinao Hashimoto
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Small 4:1795-805. 2008..g., the gas or liquid), and on variations in pressure at the flow-focusing orifices induced by the breakup of bubbles or droplets... - Using ratchets and sorters to fractionate motile cells of Escherichia coli by lengthS Elizabeth Hulme
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Lab Chip 8:1888-95. 2008..8 microm (Coefficient of Variation, CV: 21%) at the entrance, to 3.4 microm (CV: 16%) after the first sorting junction, to 3.2 mum (CV: 19%) after the second sorting junction, to 3.0 mum (CV: 19%) after the third sorting junction... - Designing ligands to bind proteinsGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Q Rev Biophys 38:385-95. 2005..The counterintuitive thermodynamic results observed serve to illustrate that, even in relatively simple systems, understanding protein-ligand association is challenging... - Continuously tunable microdroplet-laser in a microfluidic channelSindy K Y Tang
Department of Chemistry and Chemical Biology, Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts 02138, USA
Opt Express 19:2204-15. 2011..This range of tunability in wavelengths was larger than that reported in previous work on droplet-based cavities... - Influence of fluorocarbon and hydrocarbon acyl groups at the surface of bovine carbonic anhydrase II on the kinetics of denaturation by sodium dodecyl sulfateAndrew Lee
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusettes 02138, USA
J Phys Chem B 115:1199-210. 2011..These results suggested that the hydrophobicity of CF(3)CONH- is slightly greater (by a factor of <2) than that of RHCONH- with similar surface area... - Complexes of native ubiquitin and dodecyl sulfate illustrate the nature of hydrophobic and electrostatic interactions in the binding of proteins and surfactantsBryan F Shaw
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States
J Am Chem Soc 133:17681-95. 2011..This study establishes a few (of perhaps several) factors that control the simultaneous molecular recognition of multiple anionic amphiphiles by a folded cytosolic protein... - Using magnetic levitation to distinguish atomic-level differences in chemical composition of polymers, and to monitor chemical reactions on solid supportsKatherine A Mirica
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 130:17678-80. 2008..The method is particularly useful for monitoring the kinetics of reactions occurring on polymer beads... - Use of thin sectioning (nanoskiving) to fabricate nanostructures for electronic and optical applicationsDarren J Lipomi
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
Angew Chem Int Ed Engl 50:8566-83. 2011..Optical applications include surface plasmon resonators, plasmonic waveguides, and frequency-selective surfaces... - Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegansS Elizabeth Hulme
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
Lab Chip 10:589-97. 2010..This ability to perform longitudinal measurements within the device enabled the identification of age-related phenotypic changes that correlate with lifespan in C. elegans... - Electrochemical sensing in paper-based microfluidic devicesZhihong Nie
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Lab Chip 10:477-83. 2010..g., heavy-metal ions and glucose) in aqueous solutions. This low-cost analytical device should be useful for applications in public health, environmental monitoring, and the developing world... - Paper-supported 3D cell culture for tissue-based bioassaysRatmir Derda
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 106:18457-62. 2009..Cell cultures in stacked, paper-supported gels offer a uniquely flexible approach to study cell responses to 3D molecular gradients and to mimic tissue- and organ-level functions... - Nanoscience, nanotechnology, and chemistryGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Small 1:172-9. 2005 - Paper microzone platesEmanuel Carrilho
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Anal Chem 81:5990-8. 2009..Demonstration of quantitative colorimetric correlations using a scanner or camera to image the zones and to measure the intensity of color, makes it possible to conduct assays without a microplate reader... - Lysine acetylation can generate highly charged enzymes with increased resistance toward irreversible inactivationBryan F Shaw
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Protein Sci 17:1446-55. 2008.... - Molecular engineering of surfaces using self-assembled monolayersGeorge M Whitesides
Harvard University, Department of Chemistry and Chemical Biology, Cambridge, MA 02138 2902, USA
Sci Prog 88:17-48. 2005..Understanding the principles by which they form, and connecting molecular-level structure with macroscopic properties, opens a wide range of areas to study and exploitation... - Fabrication of a modular tissue construct in a microfluidic chipDerek A Bruzewicz
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Lab Chip 8:663-71. 2008..Recovery and analysis of modules after 24 h under constant flow of medium (200 microL h(-1)) showed that over 99% of encapsulated cells survived this interval in the microfluidic chamber... - Cell encapsulation in sub-mm sized gel modules using replica moldingAlison P McGuigan
Harvard University, Cambridge, Massachusetts, United States of America
PLoS ONE 3:e2258. 2008.... - Patterning multiple aligned self-assembled monolayers using lightDeclan Ryan
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Langmuir 20:9080-8. 2004..The ability to produce multiple, aligned patterns of SAMs in a single step, without alignment of photomasks in separate steps, increases the versatility of SAMs for studying a range of physical phenomena... - Self-assembled monolayers of thiolates on metals as a form of nanotechnologyJ Christopher Love
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Chem Rev 105:1103-69. 2005 - FLASH: a rapid method for prototyping paper-based microfluidic devicesAndres W Martinez
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Lab Chip 8:2146-50. 2008..FLASH provides a straightforward method for prototyping paper-based microfluidic devices in regions where the technological support for conventional photolithography is not available... - Chemistry and the worm: Caenorhabditis elegans as a platform for integrating chemical and biological researchS Elizabeth Hulme
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Angew Chem Int Ed Engl 50:4774-807. 2011..elegans that is chemically relevant. It also describes tools-biological, chemical, and physical-that are available to researchers studying the worm... - The relative rates of thiol-thioester exchange and hydrolysis for alkyl and aryl thioalkanoates in waterPaul J Bracher
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Orig Life Evol Biosph 41:399-412. 2011.... - Diagnostics for the developing world: microfluidic paper-based analytical devicesAndres W Martinez
Harvard University, Cambridge, MA 02138, USA
Anal Chem 82:3-10. 2010..To listen to a podcast about this feature, please go to the Analytical Chemistry multimedia page at pubs.acs.org/page/ancham/audio/index.html.)... - Controlling the orientation and synaptic differentiation of myotubes with micropatterned substratesJacinthe Gingras
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
Biophys J 97:2771-9. 2009..Our results represent what we believe is a new approach for musculoskeletal tissue engineering, and our model sheds light on mechanisms of myotube alignment in vivo... - Combining microscience and neurobiologyDouglas B Weibel
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Curr Opin Neurobiol 15:560-7. 2005..The major impediment to the development of a field of 'microfabrication and measurement' in neuroscience is the absence of effective collaborative interactions between the communities of fabricators and neurobiologists... - Cofabrication of electromagnets and microfluidic systems in poly(dimethylsiloxane)Adam C Siegel
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Angew Chem Int Ed Engl 45:6877-82. 2006 - Why are proteins charged? Networks of charge-charge interactions in proteins measured by charge ladders and capillary electrophoresisIrina Gitlin
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
Angew Chem Int Ed Engl 45:3022-60. 2006..By studying the influence of charge on the properties of proteins using charge ladders, it is possible to estimate the net charge and hydrodynamic radius and to infer the role of charged residues in ligand binding and protein folding... - Transistors formed from a single lithography step using information encoded in topographyMichael D Dickey
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Small 6:2050-7. 2010..The complexity of structures that can be fabricated using simple lithographic features distinguishes this procedure from other techniques based on shadow evaporation... - Multizone paper platform for 3D cell culturesRatmir Derda
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America
PLoS ONE 6:e18940. 2011..This capability made it possible to compare the growth of 3D tumor models of different spatial composition, and to examine the migration of cells in these structures... - Mechanical anisotropy of adherent cells probed by a three-dimensional magnetic twisting deviceShaohua Hu
Physiology Program, Harvard School of Public Health, Boston, MA 02115, USA
Am J Physiol Cell Physiol 287:C1184-91. 2004..Deformation patterns of the cytoskeleton and the nucleolus were sensitive to loading direction, suggesting anisotropic mechanical signaling. This technology may be useful for elucidating the structural basis of mechanotransduction... - Microfabricated teflon membranes for low-noise recordings of ion channels in planar lipid bilayersMichael Mayer
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Biophys J 85:2684-95. 2003..The low-noise properties of bilayer recordings on micropores in Teflon AF films were exploited to record the smallest conductance state of alamethicin (24 pS) at an unprecedentedly high bandwidth of 10.7 kHz... - Electrostatic self-assembly of macroscopic crystals using contact electrificationBartosz A Grzybowski
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Nat Mater 2:241-5. 2003..We suggest that the stability of these unusual structures can be explained by accounting for the interactions between electric dipoles that the particles in the aggregates induce in their neighbours... - The 'right' size in nanobiotechnologyGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Nat Biotechnol 21:1161-5. 2003.... - Components for integrated poly(dimethylsiloxane) microfluidic systemsJessamine M K Ng
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Electrophoresis 23:3461-73. 2002..Several components are described in detail: a passive chaotic mixer, pneumatically actuated switches and valves, a magnetic filter, functional membranes, and optical components... - A microfluidic apparatus for the study of ice nucleation in supercooled water dropsClaudiu A Stan
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 01238, USA
Lab Chip 9:2293-305. 2009..The dendritic growth of ice in 150-microm drops of supercooled water at -35 degrees C was observed and imaged at a rate of 16 000 frames/s... - Assumptions: taking chemistry in new directionsGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138-2902, USA
Angew Chem Int Ed Engl 43:3632-41. 2004 - Formation of droplets and bubbles in a microfluidic T-junction-scaling and mechanism of break-upPiotr Garstecki
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
Lab Chip 6:437-46. 2006.... - Chaotic mixer for microchannelsAbraham D Stroock
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Science 295:647-51. 2002..This method uses bas-relief structures on the floor of the channel that are easily fabricated with commonly used methods of planar lithography... - Don't forget long-term fundamental research in energyGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Science 315:796-8. 2007.... - Self-assembly at all scalesGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Science 295:2418-21. 2002..The concept of self-assembly is used increasingly in many disciplines, with a different flavor and emphasis in each... - Beyond molecules: self-assembly of mesoscopic and macroscopic componentsGeorge M Whitesides
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 99:4769-74. 2002..These larger systems also offer a level of control over the characteristics of the components and over the interactions among them that makes fundamental investigations especially tractable... - Folding of electrostatically charged beads-on-a-string as an experimental realization of a theoretical model in polymer scienceMeital Reches
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 106:17644-9. 2009.... - The force acting on a superparamagnetic bead due to an applied magnetic fieldSergey S Shevkoplyas
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
Lab Chip 7:1294-302. 2007..The derivation of the force on a magnetic bead due to a magnetic field also identifies the correct treatment to use for this interaction, and resolves discrepancies present throughout the literature... - Micropatterning tractional forces in living cellsNing Wang
Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115, USA
Cell Motil Cytoskeleton 52:97-106. 2002.... - Swimming in circles: motion of bacteria near solid boundariesEric Lauga
Division of Engineering and Applied Sciences, and Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02139, USA
Biophys J 90:400-12. 2006..We compare the results of the model with experimental data and obtain reasonable agreement. In particular, the radius of curvature of the trajectory is observed to increase with the length of the bacterium body... - Patterning flows using grooved surfacesAbraham D Stroock
Department of Chemistry and Chemical Biology, Harvard University, Cambridge Massachusetts 02138, USA
Anal Chem 74:5306-12. 2002..g., to control the position of streams in the cross section of a channel or to promote mixing. Potential applications in microfluidics are outlined... - Prototyping of microfluidic devices in poly(dimethylsiloxane) using solid-object printingJ Cooper McDonald
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Anal Chem 74:1537-45. 2002..The capabilities of this method are demonstrated by fabricating devices that contain multilevel and tall features, devices that cover a large area (approximately 150 cm2), and devices that contain nonintersecting, crossing channels... - Electrochemical desorption of self-assembled monolayers noninvasively releases patterned cells from geometrical confinementsXingyu Jiang
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
J Am Chem Soc 125:2366-7. 2003..This straightforward technique is useful in bioassays for drug screening and for fundamental studies in cell biology... - Rapid prototyping of microstructures by soft lithography for biotechnologyDaniel B Wolfe
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA
Methods Mol Biol 583:81-107. 2010..Here we describe the procedures for fabricating microstructures with lateral dimensions as small as 1 mum. These types of microstructures are useful for microfluidic devices, cell-based assays, and bioengineered surfaces... - The paradoxical thermodynamic basis for the interaction of ethylene glycol, glycine, and sarcosine chains with bovine carbonic anhydrase II: an unexpected manifestation of enthalpy/entropy compensationVijay M Krishnamurthy
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 128:5802-12. 2006..Thus, this study demonstrates a surprising example of enthalpy/entropy compensation in a well-defined system. Understanding this compensation is integral to the rational design of high-affinity ligands for proteins... - New approaches to nanofabrication: molding, printing, and other techniquesByron D Gates
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Chem Rev 105:1171-96. 2005 - Modeling the anodic half-cell of a low-temperature coal fuel cellDouglas B Weibel
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Angew Chem Int Ed Engl 44:5682-6. 2005 - Muscular thin films for building actuators and powering devicesAdam W Feinberg
Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
Science 317:1366-70. 2007..These centimeter-scale constructs perform functions as diverse as gripping, pumping, walking, and swimming with fine spatial and temporal control and generating specific forces as high as 4 millinewtons per square millimeter... - Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studiesSamuel K Sia
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Electrophoresis 24:3563-76. 2003..The review emphasizes the advantages of miniaturization for biological analysis, such as efficiency of the device and special insights into cell biology... - Selective chemical treatment of cellular microdomains using multiple laminar streamsShuichi Takayama
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Chem Biol 10:123-30. 2003..This technique does not require microinjection or immobilization of reagents onto nondiffusive objects; it opens a new window into cell biology... - Approaching zero: using fractured crystals in metrology for replica moldingQiaobing Xu
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 127:854-5. 2005..4 nm could be reproduced; these features approach the dimensions of atoms... - Potentiometric titrations in a poly(dimethylsiloxane)-based microfluidic deviceRosaria Ferrigno
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
Anal Chem 76:2273-80. 2004..These potentials are probed by microelectrodes that are integrated into the chip; the measured potentials were used to produce a titration curve from which the end point of a reaction was measured... - Fabrication of complex three-dimensional microchannel systems in PDMSHongkai Wu
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 125:554-9. 2003..The channels can be used as templates to form 3D structures in other materials... - Combinatorial computational method gives new picomolar ligands for a known enzymeBartosz A Grzybowski
Harvard University, Department of Chemistry and Chemical Biology, 12 Oxford Street, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 99:1270-3. 2002..The designed R isomer is the best-known inhibitor (K(d) approximately 30 pM) of human carbonic anhydrase II... - Geometric confinement influences cellular mechanical properties I -- adhesion area dependenceJudith Su
Department of Mechanical Engineering, MIT, USA
Mol Cell Biomech 4:87-104. 2007.... - Analysis by capillary electrophoresis of the kinetics of charge ladder formation for bovine carbonic anhydraseJanelle R Anderson
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Anal Chem 74:1870-8. 2002.... - Coding/decoding and reversibility of droplet trains in microfluidic networksMichael J Fuerstman
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
Science 315:828-32. 2007..The encoding/decoding device is a functional microfluidic system that requires droplets to navigate a network in a precise manner without the use of valves, switches, or other means of external control... - Geometric confinement influences cellular mechanical properties II -- intracellular variances in polarized cellsJudith Su
Department of Mechanical Engineering, MIT, USA
Mol Cell Biomech 4:105-18. 2007..9 times higher shear modulus than the trailing edge and that this increase in shear modulus correlates with a greater density of filamentous actin, as measured by phalloidin-staining observed through quantitative 3D microscopy... - Combined microfluidic-micromagnetic separation of living cells in continuous flowNan Xia
Vascular Biology Program, Department of Pathology, Karp Family Research Laboratories, Children's Hospital and Harvard Medical School, Boston, MA 02115, USA
Biomed Microdevices 8:299-308. 2006..This on-chip HGMC-microfluidic separator technology may potentially allow cell separations to be carried out in the field outside of hospitals and clinical laboratories... - Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated deviceNoo Li Jeon
Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospitals for Children, Boston, MA 02114, USA
Nat Biotechnol 20:826-30. 2002..The technique described in this paper provides a robust method to investigate migratory cells under a variety of conditions not accessible to study by earlier techniques...