Research Topics
Genomes and Genes | KATHLEEN SWEADNERSummaryAffiliation: Harvard University Country: USA Publications
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Publications
The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expressionK J Sweadner
Neuroscience Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA
Genomics 68:41-56. 2000..The gene family was named FXYD (pronounced fix-id) in recognition of invariant amino acids in its signature motif. The abundant proteins are involved in the control of ion transport...
FXYD proteins as regulators of the Na,K-ATPase in the kidneyKathleen J Sweadner
Neuroscience Center, Massachusetts General Hospital, Charlestown 02129, USA
Ann N Y Acad Sci 986:382-7. 2003..In contrast, alpha1, beta1, and gamma were found in adjacent macula densa. Phospholemman, alpha2, and beta2 are proposed to have distinct roles in regulating the sodium pump in structures involved in tubuloglomerular feedback...
Splice variants of the gamma subunit (FXYD2) and their significance in regulation of the Na, K-ATPase in kidneyElena Arystarkhova
Laboratory of Membrane Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
J Bioenerg Biomembr 37:381-6. 2005..The focus is on splice variants of gamma, their structural similarity and yet distinct effects conferred to Na, K-ATPase...
Structural similarities of Na,K-ATPase and SERCA, the Ca(2+)-ATPase of the sarcoplasmic reticulumK J Sweadner
Neuroscience Center, Massachusetts General Hospital, 149 6118, 149 13th Street, Charlestown, MA 02129, USA
Biochem J 356:685-704. 2001..On the whole, the similarity supports a shared fold, with some particular exceptions...
Predicted location and limited accessibility of protein kinase A phosphorylation site on Na-K-ATPaseK J Sweadner
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, MA 02129, USA
Am J Physiol Cell Physiol 280:C1017-26. 2001..Conditions that may expose the site or further bury it are discussed to highlight the issues facing future research on regulation of Na-K-ATPase by cAMP-dependent pathways...
Genomic organization of the human FXYD2 gene encoding the gamma subunit of the Na,K-ATPaseK J Sweadner
Neuroscience Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA
Biochem Biophys Res Commun 279:196-201. 2000..FXYD2 is on chromosome 11q23 close to a site of tumorigenic chromosomal translocations, and has a number of repeat elements...
NO regulation of Na,K-ATPase: nitric oxide regulation of the Na,K-ATPase in physiological and pathological statesDorette Z Ellis
Neuroscience Center, Massachusetts General Hospital, Charlestown 02129, USA
Ann N Y Acad Sci 986:534-5. 2003
The mechanism of Na-K interaction on Na,K-ATPaseClaudia Donnet
Laboratory of Membrane Biology, Massachusetts General Hospital, Charlestown 02129, USA
Ann N Y Acad Sci 986:249-51. 2003
A novel cAMP-stimulated pathway in protein phosphatase 2A activationMarina S Feschenko
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
J Pharmacol Exp Ther 302:111-8. 2002..Because PP2A is active toward a variety of endogenous substrates, cAMP-stimulated dephosphorylation may have complicated the interpretation of many prior studies...
Differential regulation of renal Na,K-ATPase by splice variants of the gamma subunitElena Arystarkhova
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
J Biol Chem 277:10162-72. 2002....
Global loss of Na,K-ATPase and its nitric oxide-mediated regulation in a transgenic mouse model of amyotrophic lateral sclerosisDorette Z Ellis
The Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
J Neurosci 23:43-51. 2003..Moreover, the global cellular distribution of Na,K-ATPase loss indicates that SOD1 overexpression is far-reaching in its pathological effects...
Multiplicity of expression of FXYD proteins in mammalian cells: dynamic exchange of phospholemman and gamma-subunit in response to stressElena Arystarkhova
Laboratory of Membrane Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Am J Physiol Cell Physiol 292:C1179-91. 2007..The data imply that FXYDs are obligatory rather than auxiliary components of Na-K-ATPase, and their interchangeability underlies responses of Na-K-ATPase to cellular stress...
Phospholemman, a single-span membrane protein, is an accessory protein of Na,K-ATPase in cerebellum and choroid plexusMarina S Feschenko
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
J Neurosci 23:2161-9. 2003..At least two other FXYD family members have been detected in the CNS, suggesting that additional complexity of sodium pump regulation will be found...
Phospholemman expression in extraglomerular mesangium and afferent arteriole of the juxtaglomerular apparatusRandall K Wetzel
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
Am J Physiol Renal Physiol 285:F121-9. 2003..Phospholemman is a major kinase target, and its location in the juxtaglomerular apparatus suggests that it is involved in tubuloglomerular feedback...
Distribution and oligomeric association of splice forms of Na(+)-K(+)-ATPase regulatory gamma-subunit in rat kidneyElena Arystarkhova
Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
Am J Physiol Renal Physiol 282:F393-407. 2002..Because different mechanisms maintain and regulate Na(+) homeostasis in different nephron segments, the splice forms of the gamma-subunit may have evolved to control the renal Na(+) pump through pump properties, gene expression, or both...
Hypertrophy, increased ejection fraction, and reduced Na-K-ATPase activity in phospholemman-deficient miceLi Guo Jia
Dividsion of Cardiovascular Medicine, Box 801394 MR5, University of Virginia Health System, Charlottesville, VA 22908, USA
Am J Physiol Heart Circ Physiol 288:H1982-8. 2005..We hypothesize that a primary effect of phospholemman is to modulate the Na-K-ATPase and that its reduced activity initiates compensatory responses...
Na,K-ATPase from mice lacking the gamma subunit (FXYD2) exhibits altered Na+ affinity and decreased thermal stabilityD Holstead Jones
Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 280:19003-11. 2005..Combined with other evidence that gamma participates in an early step of thermal denaturation, this indicates that FXYD proteins may play an important structural role in the enzyme complex...
Phospholemman: a new force in cardiac contractilityKathleen J Sweadner
Circ Res 97:510-1. 2005
A third mode of ouabain signaling. Focus on "Regulation of ERK1/2 by ouabain and Na-K-ATPase-dependent energy utilization and AMPK activation in parotid acinar cells"Kathleen J Sweadner
Am J Physiol Cell Physiol 295:C588-9. 2008
Research Grants
- Cellular/molecular Na,K-ATPase regulation in choroid plexusKATHLEEN SWEADNER; Fiscal Year: 2009....
- NA K ATPASE ISOZYME LOCALIZATION AND FUNCTION IN CNSKATHLEEN SWEADNER; Fiscal Year: 2001..These aims will be accomplished using a combination of cellular, molecular and immunological methodologies. ..
- STRUCTURE OF NA, K-ATPASE--MONOCLONAL ANTIBODY PROBESKATHLEEN SWEADNER; Fiscal Year: 2000..In sum, a combination of hybridoma technology, protein chemistry, and molecular approaches will be used to investigate the structure of this important plasma membrane protein. ..
- NA-K-ATPASE ISOZYME LOCALIZATION AND FUNCTION IN CNSKATHLEEN SWEADNER; Fiscal Year: 1993..This basic research should lay the groundwork for a rigorous analysis of ion movements, energy consumption, and their role in cell death and edema in ischemic brain, and contribute to a detailed understanding of ion pump function...
- Cellular/molecular Na,K-ATPase regulation in choroid plexusKathleen J Sweadner; Fiscal Year: 2010....
- Cellular/molecular Na,K-ATPase regulation in choroid plexusKATHLEEN SWEADNER; Fiscal Year: 2009....
- Cellular/molecular Na,K-ATPase regulation in choroid plexusKATHLEEN SWEADNER; Fiscal Year: 2007....
- FASEB Conference: Transport ATPasesKATHLEEN SWEADNER; Fiscal Year: 2007..The subject is related to a wide range of diseases, some of which count among the most successfully treated as a result of research. ..
- Novel Target for Ciliary Epithelium Transport RegulationKATHLEEN SWEADNER; Fiscal Year: 2005..If successful the work promises to lead to a new paradigm for understanding the regulation and pharmacological control of intraocular fluid secretion. ..
- Structure of Na,K-ATPase: monoclonal antibody probesKATHLEEN SWEADNER; Fiscal Year: 2004..In sum, a combination of protein chemistry, hybridoma technology, and molecular approaches will be used to investigate the structure of an essential plasma membrane protein. ..
- New Modulators of Na, K-ATPase in the CNSKATHLEEN SWEADNER; Fiscal Year: 2004..abstract_text> ..
- ISOZYMES OF NA, K-ATPASE--MONOCLONAL ANTIBODY PROBESKATHLEEN SWEADNER; Fiscal Year: 1993..Such physical evidence complements and tests models supported by molecular biology; for example, we have evidence that the ouabain binding site is not located at the position indicated by site-directed mutagenesis...
- ISOZYMES OF THE NA, K-ATPASE--MONOCLONAL ANTIBODY PROBESKATHLEEN SWEADNER; Fiscal Year: 1991..We will evaluate the ouabain affinities of the different Na,KATPases, to provide a basis for predicting their different physiological roles...
