Research Topics
| Andrew R OsborneSummaryAffiliation: Harvard University Country: USA Publications
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Publications
Protein translocation by the Sec61/SecY channelAndrew R Osborne
Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Cell Dev Biol 21:529-50. 2005..We review these translocation mechanisms, relating biochemical and genetic observations to the structures of the protein-conducting channel and its binding partners...
A role for the two-helix finger of the SecA ATPase in protein translocationKarl J Erlandson
Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 455:984-7. 2008....
Analysis of polypeptide movement in the SecY channel during SecA-mediated protein translocationKarl J Erlandson
Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 283:15709-15. 2008..The channel itself does not bind the polypeptide chain but provides "friction" that minimizes backsliding when ADP-bound SecA resets to "grab" the next segment of the substrate...
Ribosome binding of a single copy of the SecY complex: implications for protein translocationJean François Ménétret
Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118 2526, USA
Mol Cell 28:1083-92. 2007..We also show that point mutations of basic residues within either loop abolish ribosome binding. We suggest that SecY binds to this primary site on the ribosome and subsequently captures and translocates the nascent chain...
Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channelAndrew R Osborne
Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Cell 129:97-110. 2007..Oligomeric channels with only one active pore likely mediate protein translocation in all organisms...
RecA-like motor ATPases--lessons from structuresJiqing Ye
Department of Cell Biology, Harvard Medical School, HHMI, 240 Longwood Ave, LHRRB 613, Boston, MA 02115, USA
Biochim Biophys Acta 1659:1-18. 2004..The structures determined for different RecA-like motor ATPases begin to reveal how they move macromolecules...
A large conformational change of the translocation ATPase SecAAndrew R Osborne
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 101:10937-42. 2004..We propose that the open form of SecA represents an activated state...
