Research Topics
Genomes and Genes | Joseph ItalianoSummaryAffiliation: Harvard University Country: USA Publications
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Publications
Clinical relevance of microparticles from platelets and megakaryocytesJoseph E Italiano
Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
Curr Opin Hematol 17:578-84. 2010..This review will address mechanisms of platelet microparticle generation and evaluate our current understanding of their clinical relevance...
Selective sorting of alpha-granule proteinsJ E Italiano
Division of Translational Medicine, Brigham and Women s Hospital, Vascular Biology Program, Boston, MA 02115, USA
J Thromb Haemost 7:173-6. 2009..We consider the implications of this new paradigm of platelet secretion, discuss mechanisms of alpha-granule biogenesis, and review the molecular basis of transport and delivery of alpha-granules to assembling platelets...
Angiogenesis is regulated by a novel mechanism: pro- and antiangiogenic proteins are organized into separate platelet alpha granules and differentially releasedJoseph E Italiano
Translational Medicine Division, Brigham and Women s Hospital, Boston, MA 02115, USA
Blood 111:1227-33. 2008....
Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblastsHervé Falet
Division of Hematology, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, 221 Longwood Avenue, LMRC 301, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 99:16782-7. 2002..We conclude that Arp23 complex contribution to actin filament nucleation in platelets and fibroblasts importantly requires free barbed ends generated by severing and uncapping...
Platelets actively sequester angiogenesis regulatorsGiannoula Lakka Klement
Children s Hospital, Boston, MA 02115, USA
Blood 113:2835-42. 2009....
Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticlesRobert Flaumenhaft
Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
Blood 113:1112-21. 2009..These results indicate that direct production by megakaryocytes represents a physiologic means to generate circulating platelet microparticles...
Platelet- and megakaryocyte-derived microparticlesRobert Flaumenhaft
Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
Semin Thromb Hemost 36:881-7. 2010..Special emphasis is placed on the mechanisms of microparticle formation and novel functions for microparticles in normal physiology and disease states...
Cytoskeletal mechanisms for platelet productionJohn H Hartwig
Hematology Division, Brigham and Women s Hospital, 1 Blackfan Circle, Boston, MA 02115, USA
Blood Cells Mol Dis 36:99-103. 2006..How individual platelets form and release from proplatelets remains poorly understood...
Mechanisms and implications of platelet discoid shapeJoseph E Italiano
Division of Hematology and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
Blood 101:4789-96. 2003..Together, these findings highlight the essential requirements of the MK/platelet-restricted beta1-tubulin isoform in platelet structure and suggest that spherocytosis does not impair many aspects of platelet function...
Role for phosphoinositide 3-kinase in Fc gamma RIIA-induced platelet shape changeKurt L Barkalow
Division of Hematology, Brigham and Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
Am J Physiol Cell Physiol 285:C797-805. 2003....
The biogenesis of platelets from megakaryocyte proplateletsSunita R Patel
Hematology Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA
J Clin Invest 115:3348-54. 2005..This Review will describe the process of platelet assembly in detail and discuss several disorders that affect platelet production...
Alpha-adducin dissociates from F-actin and spectrin during platelet activationKurt L Barkalow
Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
J Cell Biol 161:557-70. 2003....
RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubulesHarald Schulze
Dana Farber Cancer Institute, Boston, MA 02115, USA
J Biol Chem 283:14109-19. 2008..GTP may influence organization of the specialized microtubules required in thrombopoiesis and that RanBP10 might serve as a molecular link between Ran and noncentrosomal microtubules...
Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubulesSunita Patel-Hett
Translational Medicine Division, Brigham and Women s Hospital, Boston, MA 02115, USA
Blood 111:4605-16. 2008..Thus, the dynamic microtubules associated with the marginal band likely function during both resting and activated platelet states...
Interactions between the megakaryocyte/platelet-specific beta1 tubulin and the secretory leukocyte protease inhibitor SLPI suggest a role for regulated proteolysis in platelet functionsHarald Schulze
Dana Farber Cancer Institute, 1 Jimmy Fund Way, Boston, MA 02115, USA
Blood 104:3949-57. 2004..SLPI may thus have unanticipated roles in MK and platelet functions, including regulated proteolysis after activation...
Delivering new insight into the biology of megakaryopoiesis and thrombopoiesisElisabeth M Battinelli
Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
Curr Opin Hematol 14:419-26. 2007..The aim of this review is to explore the state of the art knowledge on the cell biological and molecular pathways that regulate megakaryopoiesis and lead to platelet production...
Mechanisms of organelle transport and capture along proplatelets during platelet productionJennifer L Richardson
Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Blood 106:4066-75. 2005..These studies demonstrate that the components that form platelets are delivered to and assembled de novo along proplatelets...
Dynamic visualization of thrombopoiesis within bone marrowTobias Junt
Immune Disease Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
Science 317:1767-70. 2007..These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis...
Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytesSunita R Patel
Division of Hematology, Brigham and Women's Hospital, Dana Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
Blood 106:4076-85. 2005..We conclude that while continuous polymerization of microtubules is necessary to support the enlarging proplatelet mass, the sliding of overlapping microtubules is a vital component of proplatelet elongation...
ABL2/ARG tyrosine kinase mediates SEMA3F-induced RhoA inactivation and cytoskeleton collapse in human glioma cellsAkio Shimizu
Vascular Biology Program, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 283:27230-8. 2008..Cells treated with p190RhoGAP small interfering RNA also did not inactivate RhoA. Together, these results suggested that ABL2/ARG is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity...
A role for Rab27b in NF-E2-dependent pathways of platelet formationSanjay Tiwari
Dana Farber Cancer Institute, One Jimmy Fund Way, Boston, MA 02115, USA
Blood 102:3970-9. 2003..These results establish a role for Rab27 in platelet synthesis and suggest that Rab27b in particular may coordinate proplatelet formation with granule transport, possibly by recruiting specific effector pathways...
Platelet-associated PF-4 as a biomarker of early tumor growthDavid Cervi
Children s Hospital Boston, Karp Family Research Laboratories, Boston, MA 02115, USA
Blood 111:1201-7. 2008..We conclude that platelet-associated PF-4, but not its plasma counterpart, may represent a potential biomarker of early tumor presence...
Direct visualization of the endomitotic cell cycle in living megakaryocytes: differential patterns in low and high ploidy cellsNicholas Papadantonakis
Department of Biochemistry, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Cell Cycle 7:2352-6. 2008..This is the first study to document chromosomal segregation patterns during endomitosis ex vivo and to indicate their potential differential regulation in low and high ploidy cells...
Research Grants
- Cytoskeletal Mechanisms of Platelet FormationJoseph Italiano; Fiscal Year: 2005..Understanding the cytoskeletal mechanics of platelet formation may provide insights into how platelets are produced and yield strategies for treatment of thrombocytopenia. ..
- Cytoskeletal Mechanisms of Platelet FormationJoseph Italiano; Fiscal Year: 2007....
- Cytoskeletal Mechanisms of Platelet FormationJoseph E Italiano; Fiscal Year: 2010....
