ROBERT C FLAUMENHAFT

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi Proteins of the exocytotic core complex mediate platelet alpha-granule secretion. Roles of vesicle-associated membrane protein, SNAP-23, and syntaxin 4
    R Flaumenhaft
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 274:2492-501. 1999
  2. ncbi Association of oral but not transdermal estrogen therapy with enhanced platelet reactivity in a subset of postmenopausal women
    Robert Flaumenhaft
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Menopause 16:407-12. 2009
  3. ncbi Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Blood 113:1112-21. 2009
  4. ncbi Discovery-based strategies for studying platelet function
    R Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Mini Rev Med Chem 8:350-7. 2008
  5. ncbi SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet plasma membrane
    Robert Flaumenhaft
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Blood 110:1492-501. 2007
  6. ncbi Localization and quantification of platelet-rich thrombi in large blood vessels with near-infrared fluorescence imaging
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA
    Circulation 115:84-93. 2007
  7. ncbi Formation and fate of platelet microparticles
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, 41 Ave Louis Pasteur, RE319, Boston, MA 02115, USA
    Blood Cells Mol Dis 36:182-7. 2006
  8. ncbi Protein palmitoylation in signal transduction of hematopoietic cells
    Robert Flaumenhaft
    Beth Israel Deaconess Medical Center, Harvard Medical School, Division of Hemostasis and Thrombosis, Department of Medicine, Boston, MA, 02215, USA
    Hematology 10:511-9. 2005
  9. ncbi The actin cytoskeleton differentially regulates platelet alpha-granule and dense-granule secretion
    Robert Flaumenhaft
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Blood 105:3879-87. 2005
  10. ncbi Platelet- and megakaryocyte-derived microparticles
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Semin Thromb Hemost 36:881-7. 2010

Research Grants

  1. SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETION
    Robert Flaumenhaft; Fiscal Year: 2003
  2. SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETION
    Robert Flaumenhaft; Fiscal Year: 2007

Collaborators

  • Bruce Furie
  • Ann Dvorak
  • Joseph Italiano
  • John V Frangioni
  • Sidney W Whiteheart
  • Nataliya Rozenvayn
  • Derek S Sim
  • Louisa Dowal
  • Gwenda J Graham
  • Price Blair
  • James R Dilks
  • Derek Sim
  • Kamil Woronowicz
  • Eiichi Tanaka
  • Shahrokh Falati
  • Glenn Merrill-Skoloff
  • Katharine Crane Lai
  • Dian Feng
  • Lucyna Woronowicz
  • Price S Blair
  • Christian G Peters
  • Frederick Y Chen
  • Qiansheng Ren
  • Rita G Laurence
  • Peter L Gross
  • Alessandro Celi
  • Katharine Crane

Detail Information

Publications28

  1. ncbi Proteins of the exocytotic core complex mediate platelet alpha-granule secretion. Roles of vesicle-associated membrane protein, SNAP-23, and syntaxin 4
    R Flaumenhaft
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 274:2492-501. 1999
    ....
  2. ncbi Association of oral but not transdermal estrogen therapy with enhanced platelet reactivity in a subset of postmenopausal women
    Robert Flaumenhaft
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Menopause 16:407-12. 2009
    ..We sought to determine the effects of oral versus transdermal estrogen therapy on platelet function in postmenopausal women...
  3. ncbi Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Blood 113:1112-21. 2009
    ..These results indicate that direct production by megakaryocytes represents a physiologic means to generate circulating platelet microparticles...
  4. ncbi Discovery-based strategies for studying platelet function
    R Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Mini Rev Med Chem 8:350-7. 2008
    ..This review will address the evolution of discovery-based strategies to identify probes that are at once useful reagents for studying platelet activation and effective therapeutics...
  5. ncbi SNAP-23 and syntaxin-2 localize to the extracellular surface of the platelet plasma membrane
    Robert Flaumenhaft
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Blood 110:1492-501. 2007
    ..These observations represent the first description of SNARE proteins on the extracellular surface of a cell...
  6. ncbi Localization and quantification of platelet-rich thrombi in large blood vessels with near-infrared fluorescence imaging
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA
    Circulation 115:84-93. 2007
    ..Near-infrared light, with its superior tissue penetration and reduced scatter, could potentially solve this problem...
  7. ncbi Formation and fate of platelet microparticles
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, 41 Ave Louis Pasteur, RE319, Boston, MA 02115, USA
    Blood Cells Mol Dis 36:182-7. 2006
    ..The life span of platelet microparticles and the mechanism of their clearance have also not been determined. This article addresses these fundamental aspects of the physiology of platelet microparticles...
  8. ncbi Protein palmitoylation in signal transduction of hematopoietic cells
    Robert Flaumenhaft
    Beth Israel Deaconess Medical Center, Harvard Medical School, Division of Hemostasis and Thrombosis, Department of Medicine, Boston, MA, 02215, USA
    Hematology 10:511-9. 2005
    ..This review will provide an overview of the mechanisms and regulation of protein palmitoylation and focus on the role of palmitoylation in signal transduction pathways of lymphocytes and platelets...
  9. ncbi The actin cytoskeleton differentially regulates platelet alpha-granule and dense-granule secretion
    Robert Flaumenhaft
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Blood 105:3879-87. 2005
    ..These results indicate that the actin cytoskeleton interferes with platelet exocytosis and differentially regulates alpha-granule and dense granule secretion...
  10. ncbi Platelet- and megakaryocyte-derived microparticles
    Robert Flaumenhaft
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Semin Thromb Hemost 36:881-7. 2010
    ..Special emphasis is placed on the mechanisms of microparticle formation and novel functions for microparticles in normal physiology and disease states...
  11. ncbi Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+
    R Flaumenhaft
    Center for Thrombosis and Hemostasis Research, Boston, Massachusetts, USA
    J Cell Physiol 179:1-10. 1999
    ..Furthermore, these results show that H+ can trigger alpha-granule release independent of Ca2+...
  12. ncbi The platelet as a model for chemical genetics
    Robert Flaumenhaft
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Chem Biol 10:481-6. 2003
    ..The platelet is an anucleate cell with several features that render it suitable for chemical genetic analysis. This review addresses the benefits and challenges of chemical genetics using platelets as a model system...
  13. ncbi Molecular basis of platelet granule secretion
    Robert Flaumenhaft
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Mass 02115, USA
    Arterioscler Thromb Vasc Biol 23:1152-60. 2003
    ..Through these mechanisms, interactions of ligands with their cognate cell-surface receptors are transmitted to the membrane fusion machinery to facilitate membrane fusion and secretion of granule contents from platelets...
  14. ncbi The platelet actin cytoskeleton associates with SNAREs and participates in alpha-granule secretion
    Kamil Woronowicz
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Biochemistry 49:4533-42. 2010
    ..These data show that the platelet cytoskeleton interacts with select SNAREs and that actin polymerization facilitates alpha-granule release...
  15. ncbi Platelets possess and require an active protein palmitoylation pathway for agonist-mediated activation and in vivo thrombus formation
    Derek S Sim
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02115, USA
    Arterioscler Thromb Vasc Biol 27:1478-85. 2007
    ..Several platelet proteins are palmitoylated, but whether protein palmitoylation functions in platelet activation is unknown. We sought to determine the role of platelet protein palmitoylation in platelet activation and thrombus formation...
  16. ncbi Clinical relevance of microparticles from platelets and megakaryocytes
    Joseph E Italiano
    Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Curr Opin Hematol 17:578-84. 2010
    ..This review will address mechanisms of platelet microparticle generation and evaluate our current understanding of their clinical relevance...
  17. ncbi SNARE protein degradation upon platelet activation: calpain cleaves SNAP-23
    Katharine Crane Lai
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Physiol 194:206-14. 2003
    ..These studies suggest a mechanism whereby calpain activation serves to localize platelet secretion to areas of thrombus formation...
  18. ncbi Protein kinase C mediates translocation of type II phosphatidylinositol 5-phosphate 4-kinase required for platelet alpha-granule secretion
    Nataliya Rozenvayn
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 278:8126-34. 2003
    ..These results suggest a mechanism whereby protein kinase C-mediated translocation of type II PtdIns 5-phosphate 4-kinase leads to the recruitment of PtdIns (4,5)P(2)-binding proteins...
  19. ncbi Subcellular distribution of 3 functional platelet SNARE proteins: human cellubrevin, SNAP-23, and syntaxin 2
    Dian Feng
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Blood 99:4006-14. 2002
    ..This distribution provides a basis for several characteristics of alpha-granule secretion that include homotypic alpha-granule fusion and the fusion of alpha-granules with the open canalicular system and plasma membrane...
  20. ncbi In vivo models of platelet function and thrombosis: study of real-time thrombus formation
    Shahrokh Falati
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 272:187-97. 2004
  21. ncbi Targeting platelet G-protein coupled receptors (GPCRs): looking beyond conventional GPCR antagonism
    Louisa Dowal
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Curr Vasc Pharmacol 8:140-54. 2010
    ..We will also consider emerging concepts in GPCR pharmacology that have the potential to foster the development of GPCR-targeted reagents with novel mechanisms of action and improved clinical profiles...
  22. ncbi Platelet alpha-granules: basic biology and clinical correlates
    Price Blair
    Department of Medicine, Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Blood Rev 23:177-89. 2009
    ..This review will consider the formation, release, and physiologic roles of alpha-granules with special emphasis on work performed over the last decade...
  23. ncbi Endobrevin/VAMP-8-dependent dense granule release mediates thrombus formation in vivo
    Gwenda J Graham
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USA
    Blood 114:1083-90. 2009
    ..These results demonstrate the importance of dense granule release even in the earliest phases of thrombus formation and validate the distal platelet secretory machinery as a potential target for antiplatelet therapies...
  24. ncbi Phosphatidylinositol 4,5-bisphosphate regulates activation-induced platelet microparticle formation
    Nataliya Rozenvayn
    Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Biochemistry 44:6361-70. 2005
    ..These studies show that calpain may help limit PIP(2) formation following platelet activation and that PIP(2) content is an important determinant of platelet microparticle formation...
  25. ncbi Initial accumulation of platelets during arterial thrombus formation in vivo is inhibited by elevation of basal cAMP levels
    Derek S Sim
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, 41 Ave Louis Pasteur, Boston, MA 02115, USA
    Blood 103:2127-34. 2004
    ..Thus, the status of the intracellular signaling machinery prior to engagement of platelet receptors influences the rate of platelet accumulation during thrombus formation...
  26. ncbi Platelet permeabilization
    Robert Flaumenhaft
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 273:365-78. 2004
  27. ncbi Real-time assessment of cardiac perfusion, coronary angiography, and acute intravascular thrombi using dual-channel near-infrared fluorescence imaging
    Eiichi Tanaka
    Division of Hematology Oncology, Beth Israel Deaconess Medical Center, Boston, Mass 02215, USA
    J Thorac Cardiovasc Surg 138:133-40. 2009
    ..Our objective was to determine whether methylene blue, already US Food and Drug Administration approved for other indications, has useful near-infrared fluorescence properties for image-guided cardiac surgery...
  28. ncbi Interactions of platelets, blood-borne tissue factor, and fibrin during arteriolar thrombus formation in vivo
    Derek Sim
    Center for Hemostasis and Thrombosis Research, Harvard Medical School, Boston, MA 02115, USA
    Microcirculation 12:301-11. 2005
    ..This review discusses the role of platelet intracellular signaling, P-selectin expression on platelets, and tissue factor-bearing microparticles in thrombus formation...

Research Grants9

  1. SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETION
    Robert Flaumenhaft; Fiscal Year: 2003
    ..These studies are of fundamental importance as they will define the molecular basis of alpha-granule secretion, a phenomenon that has previously been described only morphologically. ..
  2. SNARE PROTEINS IN PLATELET ALPHA-GRANULE SECRETION
    Robert Flaumenhaft; Fiscal Year: 2007
    ..abstract_text> ..