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Genomes and GenesSpecies | S K CalderwoodSummaryAffiliation: Harvard University Country: USA Publications
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Publications
Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1B Chu
Dana Farber Cancer Institute and Joint Center for Radiation Therapy, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 271:30847-57. 1996....
Transcriptional activity of heat shock factor 1 at 37 degrees C is repressed through phosphorylation on two distinct serine residues by glycogen synthase kinase 3 and protein kinases Calpha and CzetaB Chu
Department of Adult Oncology, Dana Farber Cancer Institute and Joint Center for Radiation Therapy, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 273:18640-6. 1998..Regulation at 37 degrees C, therefore, involves the action of three protein kinase cascades that repress HSF1 through phosphorylation of serine residues 303, 307, and 363 and may promote growth by suppressing the heat shock response...
Effects of the flavonoid drug quercetin on the response of human prostate tumours to hyperthermia in vitro and in vivoA Asea
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Int J Hyperthermia 17:347-56. 2001..These experiments, thus, suggest the use of Quercetin as a hyperthermia sensitizer in the treatment of prostate carcinoma...
Repression of the HSP70B promoter by NFIL6, Ku70, and MAPK involves three complementary mechanismsD Tang
Department of Radiation Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Biochem Biophys Res Commun 280:280-5. 2001..These data indicate that the repression of the HSP70B promoter by NF-IL6, Ku70, and MAPK occurs independently of each other and involves three complementary mechanisms...
Cyclooxygenase inhibitors are potent sensitizers of prostate tumours to hyperthermia and radiationA Asea
Department of Radiation Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
Int J Hyperthermia 17:401-14. 2001..01) inhibition of hyperthemia-induced serum prostaglandin E2. These findings indicate that NSAID may have both sensitizing effects on prostate tumour growth and may function by inhibiting prostaglandin synthesis...
Regulation of heat shock gene transcription in neuronal cellsJ Tonkiss
Center for Behavioral Development, Boston University School of Medicine, Boston, MA, USA
Int J Hyperthermia 21:433-44. 2005..HSF1 transcriptional activity has been discussed in neuronal cells, concentrating on the regulation and activity of HSF1 and HSF2 and their role in HSP expression, during neurodegenerative diseases and as mediators of cell survival...
Heat shock factor 1 represses estrogen-dependent transcription through association with MTA1M A Khaleque
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 2215, USA
Oncogene 27:1886-93. 2008..Repression of estrogen-dependent transcription may contribute to the role of HSF1 in cancer...
Combined antitumor effect of radiation and ibuprofen in human prostate carcinoma cellsS T Palayoor
Joint Center for Radiation Therapy, Harvard Medical School, Boston, Massachusetts 02215, USA
Clin Cancer Res 4:763-71. 1998....
Targeting HSP70-induced thermotolerance for design of thermal sensitizersS K Calderwood
Department of Radiation Oncology, Dana Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA
Int J Hyperthermia 18:597-608. 2002..This paper will concentrate on the molecular pathways of thermotolerance induction for designing inhibitors of heat resistance/thermal sensitizers, which may allow the full potential of thermal therapy to be utilized...
Evolving connections between molecular chaperones and neuronal functionS K Calderwood
Division of Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Int J Hyperthermia 21:375-8. 2005
Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cellsS L Nunes
Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Biochem Biophys Res Commun 213:1-6. 1995..These experiments show a profound effect of heat shock on the structure and stability of HSF-1 complexes during nuclear localization and support the hypothesis that HSC70 binding may control HSF-1 function...
Telomerase deficiency and telomere dysfunction inhibit mammary tumors induced by polyomavirus middle T oncogeneM Jaskelioff
Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA
Oncogene 28:4225-36. 2009..Together, these results indicate that, in the setting of viral oncoprotein mammary tumorigenesis, telomerase-dependent telomere maintenance facilitates the formation and metastatic progression of mammary tumors...
Development of an XTT tetrazolium salt-based assay for detection of specific hyperthermia sensitizers in a high-flux screening programmeS Lechpammer
Department of Radiation Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
Int J Hyperthermia 18:203-15. 2002....
Heat shock proteins in cancer: chaperones of tumorigenesisStuart K Calderwood
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Trends Biochem Sci 31:164-72. 2006....
The inhibition of LPS-induced production of inflammatory cytokines by HSP70 involves inactivation of the NF-kappaB pathway but not the MAPK pathwaysYongzhong Shi
Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, People s Republic of China
Shock 26:277-84. 2006....
Extracellular heat shock proteins in cell signalingStuart K Calderwood
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
FEBS Lett 581:3689-94. 2007..The possession of a wide range of receptors for the Hsp and Grp family permits binding to a diverse range of cells and the performance of complex multicellular functions particularly in immune cells and neurones...
The anti-inflammatory effects of heat shock protein 72 involve inhibition of high-mobility-group box 1 release and proinflammatory function in macrophagesDaolin Tang
Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Changsha, People s Republic of China
J Immunol 179:1236-44. 2007..Our experimental data provide important insights into the anti-inflammatory mechanisms of heat shock protein protection...
Heat shock proteins in physiology and pathology: the Berlin meetingAlexzander A A Asea
Scott and White Memorial Hospital and Clinic, Texas A and M University Health Science Center, Temple, TX 76508, USA
Cell Stress Chaperones 12:205-8. 2007
Enhanced immunogenicity of heat shock protein 70 peptide complexes from dendritic cell-tumor fusion cellsYutaka Enomoto
Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
J Immunol 177:5946-55. 2006..These experiments indicate that HSP70-peptide complexes (PC) derived from DC-tumor fusion cells have increased their immunogenicity and therefore constitute an improved formulation of chaperone protein-based tumor vaccine...
Cell stress proteins: novel immunotherapeuticsStuart K Calderwood
Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
Novartis Found Symp 291:115-31; discussion 131-40. 2008..HSP-antigen complexes have proven effective in the treatment of rodent tumours in preclinical studies and are now undergoing clinical trials for treatment of human cancer...
Cell surface receptors for molecular chaperonesStuart K Calderwood
Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Room 553, 21 27 Burlington Avenue, Boston, MA 02215, USA
Methods 43:199-206. 2007..Understanding how heat shock protein-receptor binding orchestrates individual components of tumor immunity will permit enhanced design of molecular chaperone based immunotherapy...
Cell fusion: from hybridoma to dendritic cell-based vaccineJianlin Gong
Department of Medicine, Boston University Medical School, Boston, MA 02118, USA
Expert Rev Vaccines 7:1055-68. 2008..In addition, we discuss the challenges facing effective fusion cell vaccine production, including factors in preparation, selection and quality control of fusion cell vaccines, as well as approaches for enhancing anti-tumor immunity...
Extracellular heat shock proteins in cell signaling and immunityStuart K Calderwood
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Ann N Y Acad Sci 1113:28-39. 2007..The possession of a wide range of receptors for the Hsp and Grp family permits binding to a diverse range of cells and the performance of complex multicellular functions particularly in immune cells and neurons...
Induction of hsp70-mediated Th17 autoimmunity can be exploited as immunotherapy for metastatic prostate cancerTimothy Kottke
Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota 55902, USA
Cancer Res 67:11970-9. 2007....
Heat-shock proteins in cancer vaccines: agents of antigen cross-presentationAyesha Murshid
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Expert Rev Vaccines 7:1019-30. 2008....
The dual immunoregulatory roles of stress proteinsAlan Graham Pockley
School of Medicine and Biomedical Sciences, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK
Trends Biochem Sci 33:71-9. 2008....
OSU-03012 stimulates PKR-like endoplasmic reticulum-dependent increases in 70-kDa heat shock protein expression, attenuating its lethal actions in transformed cellsMargaret A Park
Department of Biochemistry, 401 College Street, Massey Cancer Center, Room 280a, Box 980035, Virginia Commonwealth University, Richmond, VA 23298 0035, USA
Mol Pharmacol 73:1168-84. 2008..OSU-03012 also increased expression of HSP70 in a PERK-dependent fashion, providing support for the contention that OSU-03012-induced PERK signaling promotes both cell survival and cell death processes...
Heat shock proteins: stress proteins with Janus-like properties in cancerStuart K Calderwood
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Int J Hyperthermia 24:31-9. 2008..Immunological approaches aim at increasing HSP levels in cells and tissues with the aim of increasing tumor antigen presentation to the immune system...
How is the immune response affected by hyperthermia and heat shock proteins?Stuart K Calderwood
Molecular and Cellular Radiation Oncology, BIDMC, Harvard Medical School, Boston, MA 02215, USA
Int J Hyperthermia 21:713-6. 2005..Understanding these process may permit the effective deployment of hyperthermia and hsp based vaccines in tumor treatment...
Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 bindingXiaoZhe Wang
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
J Biol Chem 281:782-91. 2006..These experiments indicate a novel mechanism for the regulation of HSF1 by proinflammatory signaling and may permit HSF1 to respond rapidly to extracellular events, permitting optimal physiological regulation...
Message in a bottle: role of the 70-kDa heat shock protein family in anti-tumor immunityStuart K Calderwood
Division of Molecular and Cellular Radiation Oncology, BIDMC, Harvard Medical School, Boston, MA 02215, USA
Eur J Immunol 35:2518-27. 2005..HSP70-PC thus coordinately activate innate immune responses and deliver antigens for re-presentation by MHC class I and II molecules on the APC cell surface, leading to specific anti-tumor immunity...
Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1XiaoZhe Wang
Dana Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 23:6013-26. 2003..Our experiments indicate a novel pathway for HSF1 regulation and suggest a mechanism for suppression of its activity during cellular proliferation...
Elevated expression of heat shock factor (HSF) 2A stimulates HSF1-induced transcription during stressHaiying He
Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 278:35465-75. 2003..Our data suggest a mechanism involving the formation of heterocomplexes between HSF1 and HSF2 with enhanced activity to activate the hsp70 promoter when compared with HSF1 or HSF2 homotrimers...
Transcriptional activity and DNA binding of heat shock factor-1 involve phosphorylation on threonine 142 by CK2Fabrice Soncin
Centre National de la Recherche Scientifique EP 560, Institut Pasteur de Lille, 1 rue Calmette BP 245, 59021, Lille Cedex, France
Biochem Biophys Res Commun 303:700-6. 2003..Phosphorylation by CK2 on threonine 142 may therefore be an essential step in the thermal activation of latent HSF1 by stresses...
Heat shock factor 1 contains two functional domains that mediate transcriptional repression of the c-fos and c-fms genesYue Xie
Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 278:4687-98. 2003..Mapping the structural domains involved in this process should permit further characterization of molecular mechanisms that mediate repression...
Tumor-derived heat shock protein 70 peptide complexes are cross-presented by human dendritic cellsElfriede Noessner
Clinical Cooperation Group Hyperthermie, Institute of Molecular Immunology, GSF National Research Center for Environment and Health, 81377 Munich, Germany
J Immunol 169:5424-32. 2002....
Double-stranded RNA-dependent protein kinase (pkr) is essential for thermotolerance, accumulation of HSP70, and stabilization of ARE-containing HSP70 mRNA during stressMeijuan Zhao
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 277:44539-47. 2002....
NF-IL6 and HSF1 have mutually antagonistic effects on transcription in monocytic cellsYue Xie
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA
Biochem Biophys Res Commun 291:1071-80. 2002..Our studies suggest a strong mutual antagonism between the heat shock response and APR, which may influence the sensitivity and duration of inflammatory responses...
Heat shock factor 1 represses transcription of the IL-1beta gene through physical interaction with the nuclear factor of interleukin 6Yue Xie
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Beth Israel and Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 277:11802-10. 2002..Interaction between HSF1 and NF-IL6 could thus be an important mechanism in HSF1 regulation of general gene transcription during endotoxin stress...
Expression of a dominant negative heat shock factor-1 construct inhibits aneuploidy in prostate carcinoma cellsYiqun Wang
Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 279:32651-9. 2004..Our experiments therefore demonstrate that HSF1 plays a functional role in cancer cells under nonstress conditions and influences cell cycle behavior and progression through mitosis and promotes the development of the aneuploid state...
Interactions between extracellular signal-regulated protein kinase 1, 14-3-3epsilon, and heat shock factor 1 during stressXiaoZhe Wang
Dana Farber Cancer Institute and Division of Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 279:49460-9. 2004..Association of HSF1 with ERK and 14-3-3epsilon during heat shock may thus modulate the amplitude of the response and lead to efficient termination of HSP expression on resumption of growth conditions...
Chaperones and slow death--a recipe for tumor immunotherapyStuart K Calderwood
Molecular and Cellular Radiation Oncology, Department of Radiation Oncology, Beth Israel Deaconess Hospital, Harvard Medical School, Boston, MA 02215, USA
Trends Biotechnol 23:57-9. 2005....
Extracellular HSP70 binding to surface receptors present on antigen presenting cells and endothelial/epithelial cellsJimmy R Theriault
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 21 27 Burlington Avenue, Boston, MA 02215, USA
FEBS Lett 579:1951-60. 2005..In addition to APC, HSP70 can avidly bind to non-APC cell lines, especially those from epithelial or endothelial background...
Expression of heat shock proteins and heat shock protein messenger ribonucleic acid in human prostate carcinoma in vitro and in tumors in vivoDan Tang
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Cell Stress Chaperones 10:46-58. 2005....
Regulatory interfaces between the stress protein response and other gene expression programs in the cellStuart K Calderwood
Division of Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 21 27 Burlington Avenue, Boston, MA 02215, USA
Methods 35:139-48. 2005..The expression of the stress protein response during periods of acute protein damage was shown to lead to efficient activation of HSF1 and HSP expression accompanied by repression of other gene expression programs...
Heat shock proteins in cancer: diagnostic, prognostic, predictive, and treatment implicationsDaniel R Ciocca
Oncology Laboratory, Institute of Experimental Medicine and Biology of Cuyo (CRICYT-CONICET, Mendoza, Argentina
Cell Stress Chaperones 10:86-103. 2005..In conclusion, the present times are of importance for the field of Hsps in cancer, with great contributions to both basic and clinical cancer research...
Induction of heat shock proteins by heregulin beta1 leads to protection from apoptosis and anchorage-independent growthMd Abdul Khaleque
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Oncogene 24:6564-73. 2005..HSF1 activated by this pathway plays a key role in the protection of cells from apoptosis and the mediation of anchorage independent growth by HRGbeta1, indicating a role for HSF1 in this tumorigenic pathway...
Novel signal transduction pathway utilized by extracellular HSP70: role of toll-like receptor (TLR) 2 and TLR4Alexzander Asea
Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 277:15028-34. 2002....
Heat induced release of Hsp70 from prostate carcinoma cells involves both active secretion and passive release from necrotic cellsSalamatu S Mambula
Division of Molecular and Cellular Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
Int J Hyperthermia 22:575-85. 2006..CONCLUSIONS: Thus, at least two mechanisms contribute to HSP70 release during hyperthermia and the relative contribution from each pathway depends on the temperature conditions...
Heat shock protein 70 is secreted from tumor cells by a nonclassical pathway involving lysosomal endosomesSalamatu S Mambula
Division of Molecular and Cellular Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 21-27 Burlington Avenue, Boston, MA 02215, USA
J Immunol 177:7849-57. 2006..Our experiments therefore suggest a mechanism for HSP70 secretion and binding to the surface of other cells that may be involved in recognition of the tumor cells by the immune system...
Targeting Cdc37 inhibits multiple signaling pathways and induces growth arrest in prostate cancer cellsPhillip J Gray
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
Cancer Res 67:11942-50. 2007..Thus, Cdc37 is essential for maintaining prostate tumor cell growth and may represent a novel target in the search for multitargeted therapies based on the HSP90 chaperone system...
Mechanisms for Hsp70 secretion: crossing membranes without a leaderSalamatu S Mambula
Division of Molecular and Cellular Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 21 27 Burlington Avenue, Room 553, Boston, MA 02215, USA
Methods 43:168-75. 2007..Thus an outline is beginning to of the mechanisms of Hsp70 secretion. Much further study will be required to fully elucidate mechanisms involved in targeting Hsp70 towards the non-canonical secretion pathways and its regulation...
