M Gawlitzek

Summary

Affiliation: Genentech Inc

Country: USA

Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms
M Gawlitzek
Process Sciences, Genentech, Inc, South San Francisco, California 94080, USA
Biotechnol Bioeng 68:637-46. 2000
Identification of cell culture conditions to control N-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells
Martin Gawlitzek
Manufacturing Sciences and Technology, Genentech, Inc, One DNA Way, South San Francisco, California 94080, USA
Biotechnol Bioeng 103:1164-75. 2009
Multiple cell culture factors can affect the glycosylation of Asn-184 in CHO-produced tissue-type plasminogen activator
D C Andersen
Department of Manufacturing Sciences, Genentech, Inc, One DNA Way, South San Francisco, California 94080 4990, USA
Biotechnol Bioeng 70:25-31. 2000

Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms
M Gawlitzek
Process Sciences, Genentech, Inc, South San Francisco, California 94080, USA
Biotechnol Bioeng 68:637-46. 2000
..Consequently, ammonium seems to alter the carbohydrate biosynthesis of TNFR-IgG by a pH-mediated effect on glycosyltransferase activity...
Identification of cell culture conditions to control N-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells
Martin Gawlitzek
Manufacturing Sciences and Technology, Genentech, Inc, One DNA Way, South San Francisco, California 94080, USA
Biotechnol Bioeng 103:1164-75. 2009
..Furthermore, the identification of relevant factors will enable one to control product quality attributes, specifically N-glycan site-occupancy, within a specific range when applied appropriately...
Multiple cell culture factors can affect the glycosylation of Asn-184 in CHO-produced tissue-type plasminogen activator
D C Andersen
Department of Manufacturing Sciences, Genentech, Inc, One DNA Way, South San Francisco, California 94080 4990, USA
Biotechnol Bioeng 70:25-31. 2000
....