Research Topics
Genomes and Genes | Jang Yen WuSummaryAffiliation: Florida Atlantic University Country: USA Publications
Research Grants
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Detail Information
Publications
Mechanism of neuroprotective function of taurineJang Yen Wu
Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL, USA
Adv Exp Med Biol 643:169-79. 2009....
Role of taurine in the central nervous systemJang Yen Wu
Charles E, Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
J Biomed Sci 17:S1. 2010..The presence of metabotropic taurine receptors which are negatively coupled to phospholipase C (PLC) signaling pathway through inhibitory G proteins is proposed, and the evidence supporting this notion is also presented...
Protective function of taurine in glutamate-induced apoptosis in cultured neuronsRebecca Leon
Department of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA
J Neurosci Res 87:1185-94. 2009..The antiapoptotic function of taurine is due to its inhibition of glutamate-induced membrane depolarization...
Role of glutamate decarboxylase (GAD) isoform, GAD65, in GABA synthesis and transport into synaptic vesicles-Evidence from GAD65-knockout mice studiesHeng Wu
Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
Brain Res 1154:80-3. 2007..However, GAD67 could serve this role under some pathological conditions...
Protein phosphorylation of human brain glutamic acid decarboxylase (GAD)65 and GAD67 and its physiological implicationsJianning Wei
Department of Biomedical Sciences, Florida Atlantic University, Boca Raton, Florida 33431, USA
Biochemistry 43:6182-9. 2004..Furthermore, mutation of T91 to aspartic acid or glutamic acid mimics the effect of phosphorylation. A model depicting the effect of phosphorylation on GAD activity upon neuronal stimulation is also proposed...
Role of mu-calpain in proteolytic cleavage of brain L-glutamic acid decarboxylaseDi Sha
Department of Biomedical Sciences, Florida Atlantic University, Boca Raton, FL 33431, USA
Brain Res 1207:9-18. 2008..mu-calpain is activated by neuronal stimulation and Ca(2+)-influx. The physiological significance of calpain in regulation of GABA synthesis and GABAergic neurotransmission is also discussed...
Genistein and daidzein induce neurotoxicity at high concentrations in primary rat neuronal culturesYing Jin
Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
J Biomed Sci 14:275-84. 2007....
Protection of taurine and granulocyte colony-stimulating factor against excitotoxicity induced by glutamate in primary cortical neuronsChunliu Pan
Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, FL 33431, USA
J Biomed Sci 17:S18. 2010..However, the mechanisms of their underlying neuroprotective effects are not fully understood...
Identification and functional analysis of truncated human glutamic acid decarboxylase 65Jianning Wei
Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
J Biomed Sci 10:617-24. 2003....
Calpain cleavage of brain glutamic acid decarboxylase 65 is pathological and impairs GABA neurotransmissionChandana Buddhala
Department of Biomedical Science, Charles E Schmidt College of Medicine, Florida Atlantic University, Boca Raton, Florida, United States of America
PLoS ONE 7:e33002. 2012....
Post-translational regulation of L-glutamic acid decarboxylase in the brainJianning Wei
Department of Basic Science, Charles E Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
Neurochem Res 33:1459-65. 2008..The results from these studies have improved our understanding of the regulation of GAD function in the brain...
Structural and functional analysis of cysteine residues in human glutamate decarboxylase 65 (GAD65) and GAD67Jianning Wei
Department of Biomedical Sciences, Florida Atlantic University, Boca Raton, Florida 33431, USA
J Neurochem 93:624-33. 2005..3702,a mass increase of 125.1 daltons (Da) as a result of modification of cysteine by NEM. Parallel studies have also been conducted with hGAD67. Cysteine 455 was found to be important for GAD67 activity...
Post-MPTP treatment with granulocyte colony-stimulating factor improves nigrostriatal function in the mouse model of Parkinson's diseaseMark McCollum
Department of Basic Science, Charles E Schmidt College of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA
Mol Neurobiol 41:410-9. 2010..This might indicate an indirect effect of G-CSF on cell proliferation. The underlying mechanism of G-CSF is under further investigation...
Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stressChunliu Pan
Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, FL 33431, USA
J Biomed Sci 17:S17. 2010..Taurine is a free amino acid present in high concentrations in a variety of organs of mammalians. As an antioxidant, taurine has been found to protect cells against oxidative stress, but the underlying mechanism is still unclear...
A novel mechanism for GABA synthesis and packaging into synaptic vesiclesChandana Buddhala
Department of Basic Science, Florida Atlantic University, Boca Raton, FL 33431, United States
Neurochem Int 55:9-12. 2009....
Activity-dependent cleavage of brain glutamic acid decarboxylase 65 by calpainJianning Wei
Department of Biomedical Sciences, Florida Atlantic University, Boca Raton, Florida 33431, USA
J Neurochem 98:1688-95. 2006..Results of our in vitro cleavage assay using purified calpain and immunopurified rat GAD65 also supported the idea that GAD65 could be directly cleaved by calpain...
Molecular cloning, expression, purification, and characterization of shorter forms of human glutamic decarboxylase 67 in an E. coli expression systemDi Sha
Department of Biomedical Sciences, Florida Atlantic University, Biomedical Science Program, 777 Glades Road, Boca Raton, FL 33431-0991, USA
Brain Res Mol Brain Res 136:255-61. 2005..The cleavage of GAD67 seems to be Ca+(2)-dependent. The model for the conversion of GAD from full-length GAD to shorter forms of GAD and its physiological implications was proposed...
Effect of taurine on regulation of GABA and acetylcholine biosynthesisDi Sha
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA
Adv Exp Med Biol 526:499-505. 2003
Effect of apocalmodulin on recombinant human brain glutamic acid decarboxylaseHong Jin
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA
J Neurochem 92:739-48. 2005..The physiological significance of the interaction between GAD and ApoCaM is discussed...
Demonstration of functional coupling between dopamine synthesis and its packaging into synaptic vesiclesRong Chen
Department of Pharmacology and Toxicology, University of Kansas, Lawrence, KS, USA
J Biomed Sci 10:774-81. 2003..Hence, it is concluded that there is a general coupling mechanism between neurotransmitter synthesis and packaging of transmitter into synaptic vesicles...
Demonstration of functional coupling between gamma -aminobutyric acid (GABA) synthesis and vesicular GABA transport into synaptic vesiclesHong Jin
Departments of Molecular Biosciences and Medicinal Chemistry, University of Kansas, Lawrence, KS 66045, USA
Proc Natl Acad Sci U S A 100:4293-8. 2003..Third, VGAT and SV-associated Ca(2+)calmodulin-dependent kinase II have been found to form a protein complex with GAD. A model is also proposed to link the neuronal stimulation to enhanced synthesis and packaging of GABA into SVs...
Research Grants
- REGULATION OF GABA BIOSYNTHESIS IN THE BRAINJang Yen Wu; Fiscal Year: 2003..The success of a large scale purification of recombinant human brain GAD65 and GAD67 and the availability of subtype specific antibodies for GAD65 and GAD67, ensure the high feasibility of this proposal. ..
