Research Topics
Genomes and Genes | S ShenolikarSummaryAffiliation: Duke University Medical Center Country: USA Publications
Research Grants
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Detail Information
Publications
Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null miceRochelle Cunningham
Univ of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
Am J Physiol Renal Physiol 294:F1001-7. 2008....- N-terminal PDZ domain is required for NHERF dimerizationS Shenolikar
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
FEBS Lett 489:233-6. 2001..In PS120 cells, reversible protein phosphorylation modulated NHERF dimerization and suggested a role for NHERF dimers in hormonal signaling... - NHERF: targeting and trafficking membrane proteinsS Shenolikar
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Physiol Renal Physiol 280:F389-95. 2001..This article reviews the new evidence that implicates NHERF in wider aspects of epithelial membrane biology... - Meeting report: targeting protein phosphatases-medicines for the new milleniumS Shenolikar
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, USA
Sci STKE 2000:pe1. 2000..A second important issue presented related to identification and characterization of various phosphatase-binding proteins that regulate phosphatase action... - Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wastingS Shenolikar
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 99:11470-5. 2002..Phosphate wasting seen in the NHERF-1(-/-) null mice provided a new experimental system for defining the role of PDZ adapters in the hormonal control of ion transport and renal disease... - Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1J H Connor
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cell Biol 21:6841-50. 2001..These studies suggested that modification of the I-1/GADD34/PP1 signaling complex regulates the initiation of protein translation in mammalian tissues... - Expanding the role of NHERF, a PDZ-domain containing protein adapter, to growth regulationJ W Voltz
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, NC 27710 USA
Oncogene 20:6309-14. 2001.... - The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchangeR A Hall
Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Nature 392:626-30. 1998..Our findings indicate that agonist-dependent beta2-adrenergic receptor binding of NHERF plays a role in beta2-adrenergic receptor-mediated regulation of Na+/H+ exchange... - Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitorS Endo
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27708, USA
Biochemistry 35:5220-8. 1996..Our data point to multiple interactions between the I-1 functional domain. and the PP1 catalytic subunit that define this phosphoprotein as a physiological regulator of the type-1 protein phosphatase... - Detection of phosphorylation by enzymatic techniquesS Shenolikar
Duke University Medical Center, Durham, North Carolina, USA
Curr Protoc Protein Sci . 2001..A support protocol has been included to identify the radiolabel as (32)P(I) based on its ability to form a complex with ammonium molybdate... - Detection of phosphorylation by enzymatic techniquesS Shenolikar
Duke University Medical Center, Durham, North Carolina, USA
Curr Protoc Mol Biol . 2001..A support protocol has been included to identify the radiolabel as (32)Pi based on its ability to form a complex with ammonium molybdate... - Cystic fibrosis transmembrane conductance regulator activation is reduced in the small intestine of Na+/H+ exchanger 3 regulatory factor 1 (NHERF-1)- but Not NHERF-2-deficient miceNellie Broere
Department of Biochemistry, Erasmus University Medical Center, 3015 GE, Rotterdam, The Netherlands
J Biol Chem 282:37575-84. 2007.... - Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targetsJames W Voltz
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 282:33879-87. 2007.... - Parathyroid hormone inhibits renal phosphate transport by phosphorylation of serine 77 of sodium-hydrogen exchanger regulatory factor-1Edward J Weinman
Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
J Clin Invest 117:3412-20. 2007..These results indicate that PTH-mediated inhibition of renal phosphate transport involves phosphorylation of S77 of the NHERF-1 PDZ I domain and the dissociation of NHERF-1/Npt2a complexes... - Sodium-hydrogen exchanger regulatory factor-1 interacts with mouse urate transporter 1 to regulate renal proximal tubule uric acid transportRochelle Cunningham
Department of Medicine, University of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
J Am Soc Nephrol 18:1419-25. 2007..These findings indicate that NHERF-1 exerts a significant effect on the renal tubular reabsorption of uric acid in the mouse by modulating the BBM abundance of mURAT1 and possibly other BBM uric acid transporters... - Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient micePaola Capuano
Institute of Physiology, Univ of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Am J Physiol Cell Physiol 292:C927-34. 2007..These data suggest that NHERF1 in the proximal tubule is important for PTH-induced internalization of NaP(i)-IIa and, specifically, couples the apical PTHR to PLC... - Adenoviral expression of NHERF-1 in NHERF-1 null mouse renal proximal tubule cells restores Npt2a regulation by low phosphate media and parathyroid hormoneRochelle Cunningham
Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
Am J Physiol Renal Physiol 291:F896-901. 2006..Together, these results highlight the requirement for NHERF-1 for physiological control of Npt2a trafficking and suggest that the Npt2a/NHERF-1 complex represents a unique PTH-responsive pool of Npt2a in renal microvilli... - Longitudinal study of urinary excretion of phosphate, calcium, and uric acid in mutant NHERF-1 null miceEdward J Weinman
Department of Medicine, University of Maryland School of Medicine, Baltimore 21202, USA
Am J Physiol Renal Physiol 290:F838-43. 2006..These studies indicate defects in the renal tubule transport of phosphate, calcium, and uric acid in NHERF-1(-/-) male and female mice that could account for the increased deposition of calcium in the papilla of null mice... - Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate mediaRochelle Cunningham
Department of Medicine, Department of Veterans Affairs Medical Center, Baltimore, Maryland, USA
Am J Physiol Renal Physiol 289:F933-8. 2005..These studies also suggest that there is a blunted phosphaturic effect of PTH in cells adapted to low-phosphate media... - The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2aEdward J Weinman
Department of Medicine, Division of Nephrology, University of Maryland, School of Medicine, 22, South Greene Street, Baltimore, MD 21202, USA
J Physiol 567:27-32. 2005..These studies indicate a unique requirement for NHERF-1 in cAMP regulation of NHE3 and in the trafficking of Npt2a... - NHERF and regulation of the renal sodium-hydrogen exchanger NHE3Edward J Weinman
Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Pflugers Arch 450:137-44. 2005..In this review, we trace the discovery of NHERF-1 and NHERF-2, and update our current understanding of the relation between these proteins and the regulation and trafficking of NHE3... - Defective parathyroid hormone regulation of NHE3 activity and phosphate adaptation in cultured NHERF-1-/- renal proximal tubule cellsRochelle Cunningham
Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
J Biol Chem 279:37815-21. 2004..NHERF-1(-/-) cells also do not adapt to alterations in the phosphate content of the media indicating that the defect resides within the cells of the proximal tubule and is not dependent on systemic factors... - A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchangeEdward J Weinman
Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
Biochemistry 42:12662-8. 2003..Together, the data suggested that the PDZ interaction between the NHE3 C-terminus and a NHERF-1 PDZ domain enhanced the regulation of sodium-hydrogen exchange by cAMP-elevating hormones... - NHERF-1 is required for renal adaptation to a low-phosphate dietEdward J Weinman
Department of Medicine, Division of Nephrology, University of Maryland School of Medicine, Rm N3W143, UHM, 22 South Greene Street, Baltimore, MD 21201, USA
Am J Physiol Renal Physiol 285:F1225-32. 2003..These experiments demonstrate that full renal adaptation to a low-phosphate diet requires NHERF-1, which serves to increase BBM expression of Npt2... - Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouseJames B Wade
Dept of Physiology, 655 W, Baltimore St, Univ of Maryland, Baltimore, MD 21201, USA
Am J Physiol Cell Physiol 285:C1494-503. 2003..These findings suggest that the organized subcellular distribution of the NHERF isoforms may play a role in the specific interactions mediating physiological control of transporter function... - NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranesEdward J Weinman
Department of Medicine, Division Nephrology, University of Maryland School of Medicine, Room N3W143, UHM, 22 South Greene Street, Baltimore, MD 21201, USA
FEBS Lett 536:141-4. 2003..As the protein levels for NHE3, NHERF-2, PKA and ezrin were not changed in the NHERF-1 (-/-) BBMs, the data suggest a unique role for NHERF-1 in cAMP-mediated inhibition of NHE3 activity in the renal proximal tubule of the mouse... - Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeletonRyan T Terry-Lorenzo
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 277:46535-43. 2002..I-2 complex to actin cytoskeleton and regulate cell morphology... - Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functionsJennifer A Gibbons
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
J Biol Chem 282:21838-47. 2007..Thus, PP1 expression in yeast should facilitate future structure-function studies of this protein serine/threonine phosphatase... - The association of NHERF adaptor proteins with g protein-coupled receptors and receptor tyrosine kinasesEdward J Weinman
Department of Medicine, University of Maryland School of Medicine, and Medical Service, Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201, USA
Annu Rev Physiol 68:491-505. 2006..This review summarizes the emerging data on the biochemical mechanisms, physiologic outcomes, and potential clinical implications of the assembly and disassembly of receptor/NHERF complexes... - Targeting protein phosphatase 1 (PP1) to the actin cytoskeleton: the neurabin I/PP1 complex regulates cell morphologyCarey J Oliver
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cell Biol 22:4690-701. 2002..These studies provided new insights into the assembly and regulation of a neurabin I/PP1 complex that controls actin rearrangement to promote spine development in mammalian neurons... - Regulation of ion transport by the NHERF family of PDZ proteinsShirish Shenolikar
Department Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Physiology (Bethesda) 19:362-9. 2004....
Research Grants
- PHOSPHATASE INHIBITOR 1, AN AMPLIFIER OF CAMP SIGNALSShirish Shenolikar; Fiscal Year: 2001..These studies are relevant to targeting phosphatases to modulate hormonal responses and ameliorate metabolic dysfunctions in many human diseases. ..
- Protein Phosphatase-1-Targeting the CytoskeletonShirish Shenolikar; Fiscal Year: 2005..Elucidating neurabin-mediated PP1 signaling may define the defects in plasticity that contribute to mental retardation, epilepsy and neurodegenerative disease. ..
- Phosphatase Inhibitor-1, An Amplifier of cAMP SignalsShirish Shenolikar; Fiscal Year: 2005..2) Define the role of GADD34 in the assembly of the mammalian eIF2alpha phosphatase and the transduction of physiological signals that regulate protein synthesis and dictate the switch between cell death and survival. ..