David A Holowka

Summary

Affiliation: Cornell University
Country: USA

Publications

  1. ncbi Interactions between Fc(epsilon)RI and lipid raft components are regulated by the actin cytoskeleton
    D Holowka
    Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853 1301, USA
    J Cell Sci 113:1009-19. 2000
  2. ncbi Insights into immunoglobulin E receptor signaling from structurally defined ligands
    David Holowka
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Immunol Rev 217:269-79. 2007
  3. ncbi Biomembrane liquid-liquid phase separation and detergent resistance: a relationship strengthened
    David Holowka
    Department of Chemistry and Chemical Biology, Cornell University, Baker Laboratory, Ithaca, NY 14853 1301, USA
    Biochem J 424:e5-6. 2009
  4. ncbi IgE receptor-mediated alteration of membrane-cytoskeleton interactions revealed by mass spectrometric analysis of detergent-resistant membranes
    Xuemei Han
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 48:6540-50. 2009
  5. ncbi Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells
    Daniel R Larson
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 171:527-36. 2005
  6. ncbi Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles
    Tobias Baumgart
    School of Applied and Engineering Physics and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 104:3165-70. 2007

Research Grants

  1. ANTIGEN BINDING, CROSSLINKING & TRANSMEMBRANE SIGNALING
    David Holowka; Fiscal Year: 1992
  2. ANTIGEN BINDING, CROSSLINKING & TRANSMEMBRANE SIGNALING
    David Holowka; Fiscal Year: 2001
  3. Antigen Binding, Crosslinking and Signaling
    David Holowka; Fiscal Year: 2007
  4. ANTIGEN BINDING, CROSSLINKING & TRANSMEMBRANE SIGNALING
    David Holowka; Fiscal Year: 1993
  5. Antigen Binding, Crosslinking and Transmembrane Signaling
    David A Holowka; Fiscal Year: 2010

Collaborators

Detail Information

Publications6

  1. ncbi Interactions between Fc(epsilon)RI and lipid raft components are regulated by the actin cytoskeleton
    D Holowka
    Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853 1301, USA
    J Cell Sci 113:1009-19. 2000
    ..Our results indicate that interactions between Lyn and crosslinked IgE-Fc(epsilon)RI are regulated by stimulated F-actin polymerization, and this is best explained by a segregation of anchored raft components from more mobile ones...
  2. ncbi Insights into immunoglobulin E receptor signaling from structurally defined ligands
    David Holowka
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Immunol Rev 217:269-79. 2007
    ..Thus, studies with chemically defined oligovalent ligands show important features of IgE receptor cross-linking that regulate signaling, leading to mast cell activation...
  3. ncbi Biomembrane liquid-liquid phase separation and detergent resistance: a relationship strengthened
    David Holowka
    Department of Chemistry and Chemical Biology, Cornell University, Baker Laboratory, Ithaca, NY 14853 1301, USA
    Biochem J 424:e5-6. 2009
    ..The study by Levental et al. in this issue of the Biochemical Journal provides new insights into the relationship between liquid-liquid phase separation in these plasma membrane vesicles and detergent-resistance of cellular lipid rafts...
  4. ncbi IgE receptor-mediated alteration of membrane-cytoskeleton interactions revealed by mass spectrometric analysis of detergent-resistant membranes
    Xuemei Han
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    Biochemistry 48:6540-50. 2009
    ..We find that these changes are independent of fatty acid chain length. Our mass spectrometric analyses provide a detailed accounting of receptor-activated alterations in the plasma membrane that are regulated by the actin cytoskeleton...
  5. ncbi Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells
    Daniel R Larson
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 171:527-36. 2005
    ..They demonstrate the capacity of FCS cross-correlation analysis to investigate the mechanism of signaling-dependent protein-protein interactions in intact, living cells...
  6. ncbi Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles
    Tobias Baumgart
    School of Applied and Engineering Physics and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 104:3165-70. 2007
    ..Thus, GPMVs now provide an effective approach to characterize biological membrane heterogeneities...

Research Grants22

  1. ANTIGEN BINDING, CROSSLINKING & TRANSMEMBRANE SIGNALING
    David Holowka; Fiscal Year: 1992
    ..These studies will determine whether the features of crosslinking that are critical for signal tranduction by IgE- receptor complexes on mast cells are also important for transmembrane signaling by surface immunoglobulin on B cells...
  2. ANTIGEN BINDING, CROSSLINKING & TRANSMEMBRANE SIGNALING
    David Holowka; Fiscal Year: 2001
    ....
  3. Antigen Binding, Crosslinking and Signaling
    David Holowka; Fiscal Year: 2007
    ....
  4. ANTIGEN BINDING, CROSSLINKING & TRANSMEMBRANE SIGNALING
    David Holowka; Fiscal Year: 1993
    ..This process appears to represent a type of immunological memory, and the molecular basis for its mechanism will be examined in conjunction with the antigen binding measurements...
  5. Antigen Binding, Crosslinking and Transmembrane Signaling
    David A Holowka; Fiscal Year: 2010
    ....