Research Topics
| F ChangSummaryAffiliation: Columbia University Country: USA Publications
Research Grants
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Detail Information
Publications
Cell biology. Formins set the record straightFred Chang
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Science 297:531-2. 2002- The contractile ringFred Chang
Columbia University College of Physicians and Surgeons, Department of Microbiology, 701 W. 168th Street, Room 1404, New York, NY 10032, USA
Curr Biol 13:R692-3. 2003 - Yeasts make their markFred Chang
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York NY, 10032, USA
Nat Cell Biol 5:294-9. 2003..As many of these components are identified, a comprehensive understanding of complex pathways is beginning to emerge... - Studies in fission yeast on mechanisms of cell division site placementF Chang
Columbia University, College of Physicians and Surgeons, Microbiology Department, New York, NY, USA
Cell Struct Funct 26:539-44. 2001..tea1p may be deposited on the cell surface by microtubules and function to recruit proteins involved in making actin structures. These studies suggest how microtubules may direct the assembly of the contractile ring in animal cells... - Shaping fission yeast with microtubulesFred Chang
Columbia University, College of Physicians and Surgeons, Department of Microbiology, 701 W 168th Street, New York 10032, USA
Cold Spring Harb Perspect Biol 1:a001347. 2009..Recent advances illustrate how the spatial and temporal regulation of cell polarization integrates many elements, including historical landmarks, positive and negative controls, and competition between pathways... - Establishment of a cellular axis in fission yeastF Chang
Columbia University, Dept of Microbiology, 701 168th St, New York, NY 10032, USA
Trends Genet 17:273-8. 2001..At the cell middle, the nucleus might specify the position of the actin contractile ring and the future cell division site by positioning cytokinesis factors such as Mid1p... - Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeastA Paoletti
Columbia University, Department of Microbiology, New York, NY 10032, USA
Mol Biol Cell 11:2757-73. 2000..Thus, the mid1p broad band defines a compartment at the medial cell surface, whose localization is linked to the position of the nucleus, and whose function may be to position the plane of cell division... - Microtubule and actin-dependent movement of the formin cdc12p in fission yeastF Chang
Columbia University, Department of Microbiology, New York, New York 10032, USA
Microsc Res Tech 49:161-7. 2000..These findings suggest a potential mechanism for how the mitotic spindle positions the cell division plane in animal cells... - cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilinF Chang
Imperial Cancer Research Fund, London, United Kingdom
J Cell Biol 137:169-82. 1997.... - Movement of a cytokinesis factor cdc12p to the site of cell divisionF Chang
Columbia University, Department of Micobiology, 701 168th Street, New York, New York 10032, USA
Curr Biol 9:849-52. 1999..These findings demonstrate that a cytokinesis factor may travel on both microtubule and actin networks to the future site of cell division... - Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombeR J Pelham
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Nat Cell Biol 3:235-44. 2001..Our studies give rise to a model in which actin patches are centres for actin polymerization that drive their own movement on actin cables using Arp2/3-based actin polymerization... - A mechanism for nuclear positioning in fission yeast based on microtubule pushingP T Tran
Department of Microbiology, Columbia University, New York, New York 10032, USA
J Cell Biol 153:397-411. 2001..Computer modeling suggests that a balance of these pushing MT forces can provide a mechanism to position the nucleus at the middle of the cell... - Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeastJ M Glynn
Department of Microbiology, Columbia University, New York, NY 10032, USA
Curr Biol 11:836-45. 2001..The role of microtubule ends in cell polarity is highlighted by the finding that the cell polarity factor, tea1p, is present on microtubule plus ends and cell tips [1]... - Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell divisionB Feierbach
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th Street, New York, NY 10032, USA
Curr Biol 11:1656-65. 2001..Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons... - Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ringF Chang
Imperial Cancer Research Fund, London, UK
J Cell Sci 109:131-42. 1996..The actin ring forms medially in a beta-tubulin mutant, showing that actin ring formation and placement are not dependent on the mitotic spindle... - Regulation of a formin complex by the microtubule plus end protein tea1pBecket Feierbach
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th St, New York, NY 10032, USA
J Cell Biol 165:697-707. 2004..Thus, these experiments begin to elucidate how microtubules contribute to the proper spatial regulation of actin assembly and polarized cell growth... - Mid2p stabilizes septin rings during cytokinesis in fission yeastAna Berlin
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
J Cell Biol 160:1083-92. 2003..No genetic interactions were found between mid2 and the related gene mid1. Thus, these studies identify a new factor responsible for the proper stability and function of septins during cytokinesis... - Regulation of cytokinesis by the formin cdc12pAnn Yonetani
Department of Microbiology and Immunology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Curr Biol 20:561-6. 2010..These observations suggest that the formin cdc12p participates downstream of cell-cycle regulators in a network that drives the initiation of cytokinesis... - Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polaritySophie G Martin
Department of Microbiology, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Dev Cell 8:479-91. 2005..Our results suggest a molecular mechanism for how microtubule plus ends regulate the spatial distribution of actin assembly... - Regulation and targeting of the fission yeast formin cdc12p in cytokinesisAnn Yonetani
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Mol Biol Cell 19:2208-19. 2008..Our findings provide insights into the regulation of formin activity and the mechanisms of contractile ring dynamics and assembly... - Establishing new sites of polarization by microtubulesNicolas Minc
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701W 168th Street, New York, NY 10032, USA
Curr Biol 19:83-94. 2009..The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that exhibits polarized cell growth at cell tips. MT plus ends contact and shrink from the cell tips and contribute to polarity regulation... - Rsp1p, a J domain protein required for disassembly and assembly of microtubule organizing centers during the fission yeast cell cycleSabina Zimmerman
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA
Dev Cell 6:497-509. 2004.... - Actin dynamics in the contractile ring during cytokinesis in fission yeastRobert J Pelham
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
Nature 419:82-6. 2002..Our studies show that cytokinesis is a more dynamic process than previously thought and provide a perspective on the mechanism of cell division... - Regulation of the formin for3p by cdc42p and bud6pSophie G Martin
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
Mol Biol Cell 18:4155-67. 2007..In particular, the for3p DAD mutation suppresses the bipolar growth (NETO) defect of bud6Delta cells. These findings suggest that cdc42p and bud6p activate for3p by relieving autoinhibition... - Shaping the actin cytoskeleton using microtubule tipsRoshni Basu
Microbiology Department, Columbia University College of Physicians and Surgeons, 701 168th St, New York 10032, USA
Curr Opin Cell Biol 19:88-94. 2007..Regulators of the actin cytoskeleton, such as activators of formin and Rho GTPases, are transported to specific sites on the cortex by riding on the plus ends of microtubules... - Dynamics of the formin for3p in actin cable assemblySophie G Martin
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, New York 10032, USA
Curr Biol 16:1161-70. 2006..These filaments are largely organized with their barbed ends facing the cell tip, where for3p is thought to function in their assembly... - Regulation of actin assembly by microtubules in fission yeast cell polarityFred Chang
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Novartis Found Symp 269:59-66; discussion 66-72, 223-30. 2005..These studies begin to elucidate a molecular pathway for how microtubules contribute to the proper spatial regulation of actin assembly and polarized cell growth... - HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomesFred Chang
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
Mol Biol Cell 15:1793-801. 2004..These defects in cell division and centrosomes might account for some of the pathological effects associated with HIV-1 infection... - New end take off: regulating cell polarity during the fission yeast cell cycleSophie G Martin
Columbia University, College of Physicians and Surgeons, Department of Microbiology, New York 10032, USA
Cell Cycle 4:1046-9. 2005..Thus, the study of NETO is revealing a mechanism by which the plus ends of microtubules regulate the spatial organization of actin... - Intra-nuclear microtubules and a mitotic spindle orientation checkpointSabina Zimmerman
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA
Nat Cell Biol 6:1245-6. 2004..Here, we show that these microtubules are actually inside the nuclear envelope... - Cytokinesis and the contractile ring in fission yeastB Feierbach
Columbia University, Department of Microbiology, 701 West 168th St, New York, NY 10032, USA
Curr Opin Microbiol 4:713-9. 2001..In particular, major findings have been made in the characterization of myosins in cytokinesis, and in how the cell division site may be positioned by the nucleus... - Organization of a sterol-rich membrane domain by cdc15p during cytokinesis in fission yeastTetsuya Takeda
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th St, New York, NY 10032, USA
Nat Cell Biol 6:1142-4. 2004..Overexpression of cdc15p in interphase cells induces abnormal membrane domain formation in an actin-independent manner. We propose that cdc15p functions to organize lipid rafts at the cleavage site for cytokinesis... - Mechanisms for maintaining microtubule bundlesScott V Bratman
Microbiology Department, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Trends Cell Biol 18:580-6. 2008..As examples, we describe recent advances in understanding the assembly and maintenance of simple MT bundles in fission yeast and plant cells, which have implications for the bundles of the animal mitotic spindle... - Role of fission yeast myosin I in organization of sterol-rich membrane domainsTetsuya Takeda
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Curr Biol 15:1331-6. 2005..Because the myo1p TH1 domain may bind directly to acidic phospholipids, these findings suggest a model for how type I myosin contributes to the organization of specialized membrane domains... - Cell polarity: a new mod(e) of anchoringSophie G Martin
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W. 168th Street, New York, NY 10032, USA
Curr Biol 13:R711-3. 2003..Recent work has revealed a novel membrane-anchoring mechanism which complements the microtubule transport of the fission yeast polarity determinant tea1p to ensure its retention at the cell tip... - Cell-cycle control: don't supersize meKally Z Pan
Microbiology Department, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Curr Biol 19:R517-9. 2009..Two recent articles suggest that fission yeast cells sense their own size through the action of an intracellular gradient emanating from cell tips and a sensor at the cell middle... - Mechanical forces of fission yeast growthNicolas Minc
Department of Microbiology, Columbia University College of Physicans and Surgeons, 701 W 168th Street, New York, NY 10032, USA
Curr Biol 19:1096-101. 2009..This study develops a quantitative framework for tip cell growth and characterizes mechanisms of force generation that contribute to fungal invasion into host tissues... - Stabilization of overlapping microtubules by fission yeast CLASPScott V Bratman
Microbiology Department, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Dev Cell 13:812-27. 2007..These findings show how a MT-stabilizing factor attached to specific sites on MTs can help to generate MT structures that have both dynamic and stable components... - Asymmetric microtubule pushing forces in nuclear centeringRafael R Daga
Department of Microbiology, Columbia University College of Physicians and Surgeon, New York, New York 10032, USA
Curr Biol 16:1544-50. 2006..These studies may provide insights into mechanisms of asymmetric microtubule behaviors and force sensing in other processes such as chromosome segregation and cell polarization... - The cell-end factor pom1p inhibits mid1p in specification of the cell division plane in fission yeastNeal N Padte
Microbiology Department, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
Curr Biol 16:2480-7. 2006..Our computational and experimental results support a model in which both positive cues from the medial nucleus and negative cues from the cell tips specify the position of the division plane... - Self-organization of microtubule bundles in anucleate fission yeast cellsRafael R Daga
Microbiology Department, Columbia University College of Physicians and Surgeons, 701 168th St, New York, NY 10032, USA
Nat Cell Biol 8:1108-13. 2006..Positioning of nuclear fragments and polarity factors by these microtubules illustrates how self-organization of these bundles contributes to establishing global spatial order... - Effects of {gamma}-tubulin complex proteins on microtubule nucleation and catastrophe in fission yeastSabina Zimmerman
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Mol Biol Cell 16:2719-33. 2005..Our findings show that mto1p and gamma-TuCs affect multiple properties of MTs including nucleation, nuclear attachment, plus-end catastrophe, and minus-end shrinkage... - Dynamic positioning of the fission yeast cell division planeRafael R Daga
Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA
Proc Natl Acad Sci U S A 102:8228-32. 2005..Furthermore, movement of ring fragments together minimizes formation of multiple division sites. These dynamic mechanisms of ring positioning provide a robust coordination of nuclear and cell division... - Laser microsurgery in fission yeast; role of the mitotic spindle midzone in anaphase BAlexey Khodjakov
New York State Department of Health, Wadsworth Center, Albany 12201, USA
Curr Biol 14:1330-40. 2004..To probe the mechanism of spindle elongation, we combine fluorescence microscopy, photobleaching, and laser microsurgery in the fission yeast Schizosaccharomyces pombe... - Fission yeast cdc31p is a component of the half-bridge and controls SPB duplicationAnne Paoletti
Institut Curie, UMR144 CNRS, 75248 Paris, France
Mol Biol Cell 14:2793-808. 2003.... - Site selection for the cleavage furrow at cytokinesisDavid R Burgess
Department of Biology, Boston College, Higgins Hall, 140 Commonwealth Ave, Chestnut Hill, MA 02167 3811, USA
Trends Cell Biol 15:156-62. 2005..In this article (which is part of the Cytokinesis series), we present a summary and critique of the major models for the roles of the mitotic apparatus microtubules in stimulating furrow formation at cytokinesis... - Movement of membrane domains and requirement of membrane signaling molecules for cytokinesisMichelle M Ng
Department of Biology, Boston College, 140 Commonwealth Avenue, Chestnut Hill, Massachusetts 02467, USA
Dev Cell 9:781-90. 2005..These studies suggest that membrane domains at the cleavage furrow possess a signaling pathway that contributes to cytokinesis... - Regulation of cytokinesis by spindle-pole bodiesValentin Magidson
Wadsworth Center, Albany, NY 12201-0509, USA
Nat Cell Biol 8:891-3. 2006..Ablation of only the daughter SPB often leads to activation of the SIN on the mother SPB and successful cytokinesis. Thus, either SPB can drive cytokinesis... - The kelch repeat protein, Tea1, is a potential substrate target of the p21-activated kinase, Shk1, in the fission yeast, Schizosaccharomyces pombeHyeWon Kim
Department of Molecular Genetics and Program in Genes and Development, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
J Biol Chem 278:30074-82. 2003..Taken together, our results strongly implicate Tea1 as a potential substrate-effector of the Shk1 kinase... - Imaging green fluorescent protein fusions in living fission yeast cellsP T Tran
University of Pennsylvania, Department of Cell and Developmental Biology, 421 Curie Blvd.-BRB2/3 Room 1009, Philadelphia, PA 19104, USA
Methods 33:220-5. 2004..We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells...
Research Grants
- Mechanisms of Nuclear Positioning and Microtubule OrganizationFred Chang; Fiscal Year: 2007..This work will provide insights into the regulation of microtubules of many cell types such as epithelial and muscle cells. As centrosomal defects are prevalent in cancer, MTOC regulation is now an important area in cancer research. ..
- Microtubule Organization and Nuclear PositioningFred Chang; Fiscal Year: 2009..In this proposal we will determine how the growth and shrinkage of microtubules are regulated. These studies will provide greater understanding of how mitotic spindles work and will be relevant for human diseases such as cancer. ..
- Microtubule Organization and Nuclear PositioningFred Chang; Fiscal Year: 2010..In this proposal we will determine how the growth and shrinkage of microtubules are regulated. These studies will provide greater understanding of how mitotic spindles work and will be relevant for human diseases such as cancer. ..
- ASSEMBLY AND PLACEMENT OF THE CELL DIVISION RINGFred Chang; Fiscal Year: 2010..Mistakes, for instance, in cytokinesis are likely to contribute to the development of the cancer cell. These studies using fission yeast as a model organism are highly relevant towards understanding human cell biology and disease. ..
- ASSEMBLY AND PLACEMENT OF THE CELL DIVISION RINGFred Chang; Fiscal Year: 2006..abstract_text> ..
- ASSEMBLY AND PLACEMENT OF THE CELL DIVISION RINGFred Chang; Fiscal Year: 2002....
- ASSEMBLY AND PLACEMENT OF THE CELL DIVISION RINGFred Chang; Fiscal Year: 2010..Mistakes, for instance, in cytokinesis are likely to contribute to the development of the cancer cell. These studies using fission yeast as a model organism are highly relevant towards understanding human cell biology and disease. ..