R R Tubbs

Summary

Affiliation: Cleveland Clinic Foundation
Country: USA

Publications

  1. ncbi Fluorescence in situ hybridization for MDM2 gene amplification as a diagnostic tool in lipomatous neoplasms
    Joshua Weaver
    Pathology and Laboratory Medicine Institute, Cleveland Clinic and the Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH 44195, USA
    Mod Pathol 21:943-9. 2008
  2. ncbi Evaluation of chronic lymphocytic leukemia by oligonucleotide-based microarray analysis uncovers novel aberrations not detected by FISH or cytogenetic analysis
    Kathryn A Kolquist
    Signature Genomic Laboratories, PerkinElmer Inc, 2820 North Astor Street, Spokane, WA, 99207, USA
    Mol Cytogenet 4:25. 2011
  3. ncbi The expression of TRMT2A, a novel cell cycle regulated protein, identifies a subset of breast cancer patients with HER2 over-expression that are at an increased risk of recurrence
    David G Hicks
    Clarient Inc, Aliso Veijo, California, USA
    BMC Cancer 10:108. 2010
  4. ncbi Automation of manual components and image quantification of direct dual label fluorescence in situ hybridization (FISH) for HER2 gene amplification: A feasibility study
    Raymond R Tubbs
    Department of Anatomic and Clinical Pathology, The Cleveland Clinic Foundation and the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH 44195, USA
    Appl Immunohistochem Mol Morphol 14:436-40. 2006
  5. ncbi Genotyping of phenotypically defined cells in neoplasia: enhanced immunoFISH via tyramide signal amplification (TSA) segregates immunophenotypically-defined cell populations for gated genotyping
    Raymond R Tubbs
    Department of Clinical Pathology L11, Cleveland Clinic Foundation and the Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA
    J Mol Histol 38:129-34. 2007
  6. ncbi An approach to the validation of novel molecular markers of breast cancer via TMA-based FISH scanning
    Raymond R Tubbs
    Departments of Anatomical and Clinical Pathology, The Cleveland Clinic Foundation, The Lerner College of Medicine, Cleveland, OH 44195, USA
    J Mol Histol 38:141-50. 2007
  7. ncbi High-resolution immunophenotyping of subcellular compartments in tissue microarrays by enzyme metallography
    Raymond Tubbs
    Department of Anatomic and Clinical Pathology, The Cleveland Clinic Foundation, OH 44195, USA
    Appl Immunohistochem Mol Morphol 13:371-5. 2005
  8. ncbi Interobserver interpretative reproducibility of GOLDFISH, a first generation gold-facilitated autometallographic bright field in situ hybridization assay for HER-2/neu amplification in invasive mammary carcinoma
    Raymond Tubbs
    Department Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195 5131, USA
    Am J Surg Pathol 26:908-13. 2002
  9. ncbi The specificity of interphase FISH translocation probes in formalin fixed paraffin embedded tissue sections is readily assessed using automated staining and scoring of tissue microarrays constructed from murine xenografts
    Raymond R Tubbs
    Dept of Clinical Pathology, The Cleveland Clinic Foundation and The Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA
    J Mol Histol 38:159-65. 2007
  10. ncbi Gold-facilitated in situ hybridization: a bright-field autometallographic alternative to fluorescence in situ hybridization for detection of Her-2/neu gene amplification
    Raymond Tubbs
    Department of Anatomic Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195 5131, USA
    Am J Pathol 160:1589-95. 2002

Detail Information

Publications84

  1. ncbi Fluorescence in situ hybridization for MDM2 gene amplification as a diagnostic tool in lipomatous neoplasms
    Joshua Weaver
    Pathology and Laboratory Medicine Institute, Cleveland Clinic and the Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH 44195, USA
    Mod Pathol 21:943-9. 2008
    ....
  2. ncbi Evaluation of chronic lymphocytic leukemia by oligonucleotide-based microarray analysis uncovers novel aberrations not detected by FISH or cytogenetic analysis
    Kathryn A Kolquist
    Signature Genomic Laboratories, PerkinElmer Inc, 2820 North Astor Street, Spokane, WA, 99207, USA
    Mol Cytogenet 4:25. 2011
    ..abstract:..
  3. ncbi The expression of TRMT2A, a novel cell cycle regulated protein, identifies a subset of breast cancer patients with HER2 over-expression that are at an increased risk of recurrence
    David G Hicks
    Clarient Inc, Aliso Veijo, California, USA
    BMC Cancer 10:108. 2010
    ..Furthermore, the small but established risk of cardiac morbidity with trastuzumab therapy compels efforts towards the identification of biomarkers that might help stratify patients...
  4. ncbi Automation of manual components and image quantification of direct dual label fluorescence in situ hybridization (FISH) for HER2 gene amplification: A feasibility study
    Raymond R Tubbs
    Department of Anatomic and Clinical Pathology, The Cleveland Clinic Foundation and the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH 44195, USA
    Appl Immunohistochem Mol Morphol 14:436-40. 2006
    ..Operational gains and enhanced consistency are inherent in this automated approach to HER2 clinical FISH testing...
  5. ncbi Genotyping of phenotypically defined cells in neoplasia: enhanced immunoFISH via tyramide signal amplification (TSA) segregates immunophenotypically-defined cell populations for gated genotyping
    Raymond R Tubbs
    Department of Clinical Pathology L11, Cleveland Clinic Foundation and the Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA
    J Mol Histol 38:129-34. 2007
    ..Enhanced immunoFISH (TSA) successfully segregates immunophenotypically-defined cell populations for gated genotyping...
  6. ncbi An approach to the validation of novel molecular markers of breast cancer via TMA-based FISH scanning
    Raymond R Tubbs
    Departments of Anatomical and Clinical Pathology, The Cleveland Clinic Foundation, The Lerner College of Medicine, Cleveland, OH 44195, USA
    J Mol Histol 38:141-50. 2007
    ....
  7. ncbi High-resolution immunophenotyping of subcellular compartments in tissue microarrays by enzyme metallography
    Raymond Tubbs
    Department of Anatomic and Clinical Pathology, The Cleveland Clinic Foundation, OH 44195, USA
    Appl Immunohistochem Mol Morphol 13:371-5. 2005
    ..This preliminary study shows the utility of using enzyme metallography for high-resolution immunophenotyping in TMAs...
  8. ncbi Interobserver interpretative reproducibility of GOLDFISH, a first generation gold-facilitated autometallographic bright field in situ hybridization assay for HER-2/neu amplification in invasive mammary carcinoma
    Raymond Tubbs
    Department Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195 5131, USA
    Am J Surg Pathol 26:908-13. 2002
    ..The GOLDFISH assay was specifically designed for qualitative interpretation, thus obviating the need for oil immersion microscopy and signal enumeration, and its interpretation was highly reproducible among five pathologists...
  9. ncbi The specificity of interphase FISH translocation probes in formalin fixed paraffin embedded tissue sections is readily assessed using automated staining and scoring of tissue microarrays constructed from murine xenografts
    Raymond R Tubbs
    Dept of Clinical Pathology, The Cleveland Clinic Foundation and The Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195, USA
    J Mol Histol 38:159-65. 2007
    ..Moderate complexity tissue microarrays constructed from murine xenografts are excellent validation tools for initial assessment of interphase FISH probe specificity...
  10. ncbi Gold-facilitated in situ hybridization: a bright-field autometallographic alternative to fluorescence in situ hybridization for detection of Her-2/neu gene amplification
    Raymond Tubbs
    Department of Anatomic Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195 5131, USA
    Am J Pathol 160:1589-95. 2002
    ..Results correlated well with indirect and direct label FISH. Autometallographic gold-based in situ hybridization represents a promising bright-field assay for the assessment of HER-2/neu gene amplification...
  11. ncbi Fluorescence in situ hybridization (FISH) as primary methodology for the assessment of HER2 Status in adenocarcinoma of the breast: a single institution experience
    Raymond R Tubbs
    Anatomic and Clinical Pathology, Cleveland Clinic Foundation and the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, USA
    Diagn Mol Pathol 16:207-10. 2007
    ..0 or more in at least 80% of tumor cells, was observed in 3% (20/742) of the cases. These data may be helpful as benchmarks for other institutions initiating primary FISH analysis for HER2 genotyping...
  12. ncbi Outcome of patients with early-stage breast cancer treated with doxorubicin-based adjuvant chemotherapy as a function of HER2 and TOP2A status
    Raymond Tubbs
    Department of Molecular Pathology, Cleveland Clinic, Cleveland, OH 44195, USA
    J Clin Oncol 27:3881-6. 2009
    ..We determined the status of the HER2 and TOP2A genes in a large cohort of breast cancer patients treated with adjuvant doxorubicin (A) and cyclophosphamide (C)...
  13. ncbi Concomitant oncoprotein detection with fluorescence in situ hybridization (CODFISH): a fluorescence-based assay enabling simultaneous visualization of gene amplification and encoded protein expression
    R R Tubbs
    Department of Clinical Pathology, The Cleveland Clinic Foundation, Ohio 44195, USA
    J Mol Diagn 2:78-83. 2000
    ..In addition, qualitative immunohistochemistry may be followed by CODFISH gene copy enumeration to clarify ambiguous cases...
  14. ncbi Discrepancies in clinical laboratory testing of eligibility for trastuzumab therapy: apparent immunohistochemical false-positives do not get the message
    R R Tubbs
    Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Clin Oncol 19:2714-21. 2001
    ..However, simple overexpression by tumor cells that have normal gene copy has not been evaluated by profiling mRNA expression, ie, such cases could simply represent true-positive, transcriptionally upregulated overexpression...
  15. ncbi Aneusomy of chromosomes 7, 8, and 17 and amplification of HER-2/neu and epidermal growth factor receptor in Gleason score 7 prostate carcinoma: a differential fluorescent in situ hybridization study of Gleason pattern 3 and 4 using tissue microarray
    M Skacel
    Department of Anatomic, The Urology Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Hum Pathol 32:1392-7. 2001
    ..Chromosome 7 abnormalities may play an important role in cancer progression in margin-negative patients. EGFR amplification was rare, suggesting that this oncogene is not altered at the gene copy number level...
  16. ncbi Typical and atypical chronic lymphocytic leukemia differ clinically and immunophenotypically
    J L Frater
    Division of Pathology and Laboratory Medicine, Cleveland Clinic Foundation, OH 44106, USA
    Am J Clin Pathol 116:655-64. 2001
    ..aCLL can be distinguished from typical CLL morphologically, clinically, and immunophenotypically. Atypical morphologic features in CLL seem to be a marker of aggressive clinical behavior...
  17. ncbi Absence of CCND1 gene amplification in breast tumours of BRCA1 mutation carriers
    S A Vaziri
    Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Mol Pathol 54:259-63. 2001
    ..These data suggest that breast tumorigenesis in BRCA1 mutation carriers occurs by a molecular mechanism distinct from that of age matched non-familial cases...
  18. ncbi Angiotropic lymphoma: an immunophenotypically and clinically heterogeneous lymphoma
    S Yegappan
    Department of Clinical Pathology, Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Mod Pathol 14:1147-56. 2001
    ..Cutaneous manifestations of AL are common; however, it appears to be a systemic lymphoma. Although often fatal, patients with AL who are diagnosed early and treated with chemotherapy may achieve remission...
  19. ncbi Use of novel t(11;14) and t(14;18) dual-fusion fluorescence in situ hybridization probes in the differential diagnosis of lymphomas of small lymphocytes
    J L Frater
    Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195, USA
    Diagn Mol Pathol 10:214-22. 2001
    ..FISH can be performed successfully on archival single-cell preparations (touch preparations or gravity preparations) when fresh tissue is unavailable. No discordant or false-positive cases were identified...
  20. ncbi Interleukin 7 enhances the proliferation and effector function of tumor-infiltrating lymphocytes from renal-cell carcinoma
    D Sica
    Department of Immunology, Cleveland Clinic Foundation, OH 44195
    Int J Cancer 53:941-7. 1993
    ..These results illustrate that IL7 can potentiate the growth and production of IFN gamma from RCC-reactive TIL and, to a lesser extent, enhance IL2-induced LAK activity of TIL...
  21. ncbi Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and Histochoice
    D G Vince
    Whitaker Biomedical Imaging Laboratory, Department of Biomedical Engineering, Cleveland Clinic Foundation, Ohio 44195, USA
    Anal Cell Pathol 15:119-29. 1997
    ..7% of cases than those prepared with NBF, indicating darker staining. These results show that Histochoice produces staining intensity that is comparable, and in many cases superior, to formalin...
  22. ncbi Evaluation of a new paraffin-reactive CD7 T-cell deletion marker and a polymerase chain reaction-based T-cell receptor gene rearrangement assay: implications for diagnosis of mycosis fungoides in community clinical practice
    A Ormsby
    Department of Anatomic Pathology and Dermatology, The Cleveland Clinic Foundation, 9500 Euclid Ave, Cleveland, OH 44195, USA
    J Am Acad Dermatol 45:405-13. 2001
    ..CONCLUSION: In the diagnosis of MF, detection of CD7 deletion and monoclonal TCR rearrangements can be successfully performed in a cost-effective, timely fashion using routine formalin-fixed paraffin-embedded biopsy specimens...
  23. ncbi Immunohistochemical analysis identifies two cyclin D1+ subsets of plasma cell myeloma, each associated with favorable survival
    James R Cook
    Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Am J Clin Pathol 125:615-24. 2006
    ..005). This report shows for the first time that cyclin D1 immunohistochemical analysis can provide prognostic information in plasma cell myeloma...
  24. ncbi Validation of a multicolor interphase fluorescence in situ hybridization assay for detection of transitional cell carcinoma on fresh and archival thin-layer, liquid-based cytology slides
    M Skacel
    Department of Anatomic Pathology, Cleveland Clinic Foundation, Ohio 44195, USA
    Anal Quant Cytol Histol 23:381-7. 2001
    ..To evaluate the feasibility of performing multicolor interphase fluorescence in situ hybridization (FISH) on ThinPrep slides of transitional cell carcinoma (TCC)...
  25. ncbi HER2 status in bilateral breast cancer
    Joseph P Crowe
    Departments of General Surgery Breast Center, The Cleveland Clinic, Cleveland, Ohio 44195, USA
    Int J Fertil Womens Med 51:219-23. 2006
    ..The purpose of this study was to identify correlates of HER2 status for patients with bilateral breast cancer...
  26. ncbi Assessment of the HER2 status in breast cancer by fluorescence in situ hybridization: a technical review with interpretive guidelines
    David G Hicks
    Department of Anatomic Pathology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Hum Pathol 36:250-61. 2005
    ..The development of automated platforms for hybridization, image analysis for signal enumeration, and experience with FISH interpretation should broaden the availability of this technology for clinical diagnostic testing...
  27. ncbi Mutations in myeloid neoplasms
    Claudiu V Cotta
    Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH 44122, USA
    Diagn Mol Pathol 17:191-9. 2008
    ..In addition to the information about mutations, their significance, and the methods used in their diagnosis, the normal patterns of expression of these genes, their normal functions, and their structures are also discussed...
  28. ncbi A data model to predict HER2 status in breast cancer based on the clinical and pathologic profiles of a large patient population at a single institution
    Joseph P Crowe
    Department of General Surgery Breast Center, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A 80, Cleveland, OH 44195, USA
    Breast 15:728-35. 2006
    ..Correlates of HER2 amplification were higher Scarff-Bloom-Richardson grade, younger age at diagnosis, and a comedo ductal carcinoma in situ component...
  29. ncbi JC virus chromogenic in situ hybridization in brain biopsies from patients with and without PML
    Gary W Procop
    Division of Pathology and Laboratory Medicine, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Diagn Mol Pathol 15:70-3. 2006
    ..CISH seems to be a useful tool for the detection of the JC virus in brain biopsies from patients with PML, and is more accessible because a commercial probe is available...
  30. ncbi Fluorescence in situ hybridization analysis of immunoglobulin heavy chain translocations in plasma cell myeloma using intact paraffin sections and simultaneous CD138 immunofluorescence
    James R Cook
    Department of Clinical Pathology, L11, 9500 Euclid Ave, Cleveland, OH 44195, USA
    J Mol Diagn 8:459-65. 2006
    ..This technique allows for efficient molecular cytogenetic analysis of plasma cell myeloma using routinely archived paraffin-embedded material...
  31. ncbi Variation of monosomy 3 status within uveal melanoma
    Lynn Schoenfield
    Department of Anatomic Pathology, and the Cole Eye Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Arch Pathol Lab Med 133:1219-22. 2009
    ..Monosomy 3, one such variable, can be determined using fluorescence in situ hybridization, either on enucleated samples, fine-needle aspiration biopsy, or tumor sample obtained by vitrector...
  32. ncbi The expression of the cytoskeletal focal adhesion protein paxillin in breast cancer correlates with HER2 overexpression and may help predict response to chemotherapy: a retrospective immunohistochemical study
    Sarah M Short
    Department of Anatomic and Clinical Pathology L25, Cleveland, Ohio, USA
    Breast J 13:130-9. 2007
    ..Further study of a role for paxillin expression in predicting response to cytotoxic regimens or targeted treatments is warranted...
  33. ncbi From array to array: confirmation of genomic gains and losses discovered by array-based comparative genomic hybridization utilizing fluorescence in situ hybridization on tissue microarrays
    Marek Skacel
    Department of Anatomic Pathology, The Cleveland Clinic Foundation, L 25, Cleveland, OH 44191, USA
    J Mol Histol 38:135-40. 2007
    ..As such, this approach facilitates discovery, validation and implementation of findings in the process of identification of new diagnostic, prognostic and potentially therapeutic molecular markers...
  34. ncbi Demonstration of immune complex deposits using fluorescence microscopy of hematoxylin and eosin-stained sections of Hollande's fixed renal biopsies
    James T McMahon
    Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mod Pathol 15:988-97. 2002
    ....
  35. ncbi Detection of bcl-2/J(H) translocation by polymerase chain reaction: a summary of the experience of the Molecular Oncology Survey of the College of American Pathologist
    Eric D Hsi
    Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Arch Pathol Lab Med 126:902-8. 2002
    ....
  36. ncbi Array-based comparative genomic hybridization in ulcerative colitis neoplasia: single non-dysplastic biopsies distinguish progressors from non-progressors
    Mary P Bronner
    Division of Pathology and Laboratory Medicine, Cleveland Clinic, Cleveland, OH 44195, USA
    Mod Pathol 23:1624-33. 2010
    ..They therefore show promise toward eliminating full colonoscopic surveillance with extensive biopsy sampling in the majority of ulcerative colitis patients...
  37. ncbi Posttransplant lymphoproliferative disorders in lung transplant patients: the Cleveland Clinic experience
    P Ramalingam
    Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195, USA
    Mod Pathol 15:647-56. 2002
    ..Although the incidence is low, careful early monitoring of lung transplantation patients is warranted because of the poor prognosis of patients developing this complication...
  38. ncbi Breast tumor immunophenotype of BRCA1-mutation carriers is influenced by age at diagnosis
    S A Vaziri
    Department of Cancer Biology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Clin Cancer Res 7:1937-45. 2001
    ..Because histological features of tumors of premenopausal women differ from those of tumors of older women, we sought to determine whether the immunophenotype of breast tumors of BRCA1 mutation carriers was influenced by age at diagnosis...
  39. ncbi Calpain regulates sensitivity to trastuzumab and survival in HER2-positive breast cancer
    S Kulkarni
    Department of Molecular Pathology, Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Oncogene 29:1339-50. 2010
    ..These data identify calpain as a new therapeutic target in HER2-positive breast cancer...
  40. ncbi HER2/neu amplification in breast cancer: stratification by tumor type and grade
    Elise R Hoff
    Department of Anatomic Pathology, Cleveland Clinic Foundation, OH 44195, USA
    Am J Clin Pathol 117:916-21. 2002
    ....
  41. ncbi HER2 overexpression and amplification in urothelial carcinoma of the bladder is associated with MYC coamplification in a subset of cases
    Donna E Hansel
    Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Am J Clin Pathol 130:274-81. 2008
    ..Further studies into the role of MYC coamplification in this population are needed to determine impacts on treatment with HER2-targeted therapy...
  42. ncbi Identification of the JAK2 V617F mutation in chronic myeloproliferative disorders using FRET probes and melting curve analysis
    Gurunathan Murugesan
    Department of Clinical Pathology, Cleveland Clinic Foundation and the Cleveland Clinic, Lerner College of Medicine, Cleveland, OH 44195, USA
    Am J Clin Pathol 125:625-33. 2006
    ..This study demonstrates that clinical genotyping of the JAK2 V617F mutation can be performed by melting analysis using both freshly isolated and formalin-fixed tissues...
  43. ncbi The incidence of topoisomerase II-alpha genomic alterations in adenocarcinoma of the breast and their relationship to human epidermal growth factor receptor-2 gene amplification: a fluorescence in situ hybridization study
    David G Hicks
    Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Hum Pathol 36:348-56. 2005
    ..If TOP2A gene dosage can be confirmed to correlate with tumor responsiveness to anthracycline-based therapy in the clinical setting, FISH testing for TOP2A status may be warranted to aid in the selection of the most appropriate therapy...
  44. ncbi Out of the darkness and into the light: bright field in situ hybridisation for delineation of ERBB2 (HER2) status in breast carcinoma
    Aaron M Gruver
    Department of Molecular Pathology, Pathology and Laboratory Medicine Institute, Cleveland Clinic, Lerner College of Medicine, Cleveland, Ohio 44195, USA
    J Clin Pathol 63:210-9. 2010
    ..A summary of the advancements in bright field in situ hybridisation, with focus on those techniques with clinical applications of interest to the practicing pathologist, is presented...
  45. ncbi The influence of polysomy 17 on HER2 gene and protein expression in adenocarcinoma of the breast: a fluorescent in situ hybridization, immunohistochemical, and isotopic mRNA in situ hybridization study
    Erinn Downs-Kelly
    Department of Anatomical and Clinical Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Am J Surg Pathol 29:1221-7. 2005
    ..These results suggest that chromosome 17 polysomy in the absence of HER2 amplification does not have a significant biologic influence on HER2 gene expression in breast carcinoma...
  46. ncbi Prognostic value of regulatory T cells, lymphoma-associated macrophages, and MUM-1 expression in follicular lymphoma treated before and after the introduction of monoclonal antibody therapy: a Southwest Oncology Group Study
    J W Sweetenham
    Department of Hematologic Oncology and Blood Disorders, Cleveland Clinic Taussig Cancer Institute, Cleveland, OH, USA
    Ann Oncol 21:1196-202. 2010
    ..The purpose was to examine the prognostic impact of features of tumor cells and immune microenvironment in patients with follicular lymphoma treated with and without anti-CD20 monoclonal antibody therapy...
  47. ncbi Diffuse large B-cell lymphoma of the stomach: assessment of microsatellite instability, allelic imbalance, and trisomy of chromosomes 3, 12, and 18
    Marek Skacel
    Department of Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Diagn Mol Pathol 11:75-82. 2002
    ..Because of the relatively low frequency of MSI in the high-grade B-cell lymphomas of the stomach, this feature cannot be used to reliably discriminate between the histologic types of extranodal diffuse large B-cell lymphoma...
  48. ncbi Loss of breast cancer metastasis suppressor 1 protein expression predicts reduced disease-free survival in subsets of breast cancer patients
    David G Hicks
    Department of Clinical and Anatomic Pathology, General Surgery-Breast Center, Lerner Research Institute, The Cleveland Clinic, Cleveland, Ohio 44195, USA
    Clin Cancer Res 12:6702-8. 2006
    ..BRMS1 staining could potentially be used in patient stratification in conjunction with other prognostic markers. Further, mechanisms other than genomic deletion account for loss of BRMS1 gene expression in breast tumors...
  49. ncbi Molecular diagnostic techniques for the clinical evaluation of gliomas
    Todd W Kelley
    Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Diagn Mol Pathol 14:1-8. 2005
    ....
  50. ncbi Automated colorimetric in situ hybridization (CISH) detection of immunoglobulin (Ig) light chain mRNA expression in plasma cell (PC) dyscrasias and non-Hodgkin lymphoma
    Rose C Beck
    Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195, USA
    Diagn Mol Pathol 12:14-20. 2003
    ..Automated CISH is useful in detecting light chain expression in paraffin sections and appeared superior to IHC for light chain detection in PC dyscrasias and B cell non-Hodgkin lymphomas, predominantly due to lack of background staining...
  51. ncbi Breast cancers with brain metastases are more likely to be estrogen receptor negative, express the basal cytokeratin CK5/6, and overexpress HER2 or EGFR
    David G Hicks
    Clinical and Anatomic Pathology, Cleveland Clinic Foundation, OH 44195, USA
    Am J Surg Pathol 30:1097-104. 2006
    ....
  52. ncbi Detection of MDM2 gene amplification or protein expression distinguishes sclerosing mesenteritis and retroperitoneal fibrosis from inflammatory well-differentiated liposarcoma
    Joshua Weaver
    Department of Anatomic Pathology, Pathology and Laboratory Medicine Institute, The Cleveland Clinic, Cleveland, OH, USA
    Mod Pathol 22:66-70. 2009
    ..However, lack of MDM2 immunoexpression would rule out the possibility of inflammatory well-differentiated liposarcoma...
  53. ncbi The impact of genotype on outcome in oligodendroglioma: validation of the loss of chromosome arm 1p as an important factor in clinical decision making
    Andrew A Kanner
    The Brain Tumor Institute, The Cleveland Clinic Taussig Cancer Center, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Neurosurg 104:542-50. 2006
    ..The authors of further prospective studies may better determine the true value of the allelic loss of 1p and its implication for clinical decision making...
  54. ncbi t(14;18)(q32;q21) involving IGH and MALT1 is uncommon in cutaneous MALT lymphomas and primary cutaneous diffuse large B-cell lymphomas
    Nicholas T Wongchaowart
    Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Cutan Pathol 33:286-92. 2006
    ..However, the incidence of IGH/MALT1 translocations in these forms of cutaneous lymphoma remains unclear...
  55. ncbi The utility of FOXO1 fluorescence in situ hybridization (FISH) in formalin-fixed paraffin-embedded specimens in the diagnosis of alveolar rhabdomyosarcoma
    Erinn Downs-Kelly
    Department of Anatomic, Cleveland Clinic, Cleveland, OH 44195, USA
    Diagn Mol Pathol 18:138-43. 2009
    ....
  56. ncbi The expression of fascin, an actin-bundling motility protein, correlates with hormone receptor-negative breast cancer and a more aggressive clinical course
    Brian J Yoder
    Department of Anatomic Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Clin Cancer Res 11:186-92. 2005
    ..If so, then fascin may represent a new molecular target for therapeutic intervention in patients with ER-negative breast cancer...
  57. ncbi T lymphocytes infiltrating renal cell carcinoma have a reduced expression of transferrin receptor
    S Kudoh
    Department of Immunology, Cleveland Clinic Foundation, OH 44195
    Int J Cancer 58:369-75. 1994
    ..Our studies illustrate another alteration in T-TIL that is not observed in T cells from the peripheral blood. The absence of TfR gene expression may contribute to the poor proliferative response of T cells from the tumor...
  58. ncbi Use of in situ hybridization to detect human papillomavirus in head and neck squamous cell carcinoma patients without a history of alcohol or tobacco use
    Walter T Lee
    Head and Neck Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Arch Pathol Lab Med 132:1653-6. 2008
    ..There are, however, a group of patients without a significant history of tobacco or alcohol use, and the etiology of these tumors is incompletely understood...
  59. ncbi Extranodal marginal zone B-cell lymphomas of the ocular adnexa: multiparameter analysis of 34 cases including interphase molecular cytogenetics and PCR for Chlamydia psittaci
    Angela Ruiz
    Department of Clinical Pathology, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH 44195, USA
    Am J Surg Pathol 31:792-802. 2007
    ..The lack of C. psittaci in this series, in contrast to some prior reports, indicates that there may also be geographic heterogeneity in the pathogenesis of ocular adnexa MALT lymphoma...
  60. ncbi Utility of FISH in the diagnosis of angiomatoid fibrous histiocytoma: a series of 18 cases
    Munir R Tanas
    Department of Anatomic Pathology, Pathology and Laboratory Medicine Institute, The Cleveland Clinic and The Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH 44195, USA
    Mod Pathol 23:93-7. 2010
    ..Finally, we discuss some of the potential pitfalls of this technique, including confusion with other mesenchymal neoplasms containing rearrangement of EWSR1, in particular Ewing's sarcoma/PNET...
  61. ncbi Multitarget fluorescence in situ hybridization assay detects transitional cell carcinoma in the majority of patients with bladder cancer and atypical or negative urine cytology
    Marek Skacel
    Department of Anatomic and Clinical Pathology, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    J Urol 169:2101-5. 2003
    ..These results emphasize the important role of this assay in the management of bladder cancer...
  62. ncbi Cyclooxygenase-2 in oligodendroglial neoplasms
    Elias A Castilla
    Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Cancer 98:1465-72. 2003
    ..Tumors with expression of COX-2 by immunohistochemistry may, in theory, benefit from treatment with therapeutic agents that inhibit COX-2...
  63. ncbi Chromosomal gains and genomic loss of p53 and p16 genes in Barrett's esophagus detected by fluorescence in situ hybridization of cytology specimens
    Mona Fahmy
    Department of Anatomic and Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Mod Pathol 17:588-96. 2004
    ..Selected biomarkers from this study merit further investigation to determine their potential to detect genetic changes in patients with Barrett's esophagus prior to the development of high-grade dysplasia...
  64. ncbi The utility of fluorescence in situ hybridization (FISH) in the diagnosis of myxoid soft tissue neoplasms
    Erinn Downs-Kelly
    Department of Anatomic Pathology, Cleveland Clinic, Cleveland, OH 44195, USA
    Am J Surg Pathol 32:8-13. 2008
    ..FISH is capable of providing specific ancillary information useful in this often difficult differential diagnosis...
  65. ncbi Tissue microarrays: a powerful tool for high-throughput analysis of clinical specimens: a review of the method with validation data
    Marek Skacel
    Cleveland Clinic Foundation Department of Anatomic Pathology, Ohio 44195, USA
    Appl Immunohistochem Mol Morphol 10:1-6. 2002
    ....
  66. ncbi Bone marrow phospho-STAT5 expression in non-CML chronic myeloproliferative disorders correlates with JAK2 V617F mutation and provides evidence of in vivo JAK2 activation
    Samer Aboudola
    Department of Clinical Pathology and Laboratory Medicine, Taussig Cancer Center, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Am J Surg Pathol 31:233-9. 2007
    ..Phosphoprotein detection represents a new area for diagnostic pathology that exploits specific functional characteristics of cells within the context of a tissue section...
  67. ncbi Chromosome 1p allelic loss by fluorescence in situ hybridization is not observed in dysembryoplastic neuroepithelial tumors
    Richard A Prayson
    Department of Neurological Surgery, Cleveland Clinic Foundation, OH 44195, USA
    Am J Clin Pathol 118:512-7. 2002
    ..LOH on chromosome 1p may be a useful differential diagnostic feature (favoring oligodendroglioma) in a subset of cases in which specimen fragmentation or size raises the differential diagnosis of DNT vs oligodendroglioma...
  68. ncbi Reflex UroVysion testing of bladder cancer surveillance patients with equivocal or negative urine cytology: a prospective study with focus on the natural history of anticipatory positive findings
    Brian J Yoder
    Department of Anatomic and Clinical Pathology, Cleveland Clinic Foundation, leveland, OH 44195, USA
    Am J Clin Pathol 127:295-301. 2007
    ..In about 65% of this anticipatory positive group, recurrent bladder urothelial carcinoma developed within 29 months...
  69. ncbi Interphase fluorescence in-situ hybridization in the diagnosis of bladder cancer
    Marek Skacel
    Department of Anatomic and Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Front Biosci 7:e27-32. 2002
    ..Therefore, this technique can be an important aid in the efforts to reduce mortality from transitional cell carcinoma of the bladder, since it increases our ability to prevent progression to incurable muscle invasive disease...
  70. ncbi Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant
    Anatoly Urisman
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS Pathog 2:e25. 2006
    ..These findings also raise questions about the possible relationship between exogenous infection and cancer development in genetically susceptible individuals...
  71. ncbi HER-2 fluorescence in situ hybridization: results from the survey program of the College of American Pathologists
    Diane L Persons
    Department of Pathology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Arch Pathol Lab Med 130:325-31. 2006
    ..Fluorescence in situ hybridization (FISH) is a common method used to determine HER-2 status in breast cancer. Limited information is available concerning reproducibility of FISH in determining HER-2 gene amplification...
  72. ncbi HER-2 testing in breast cancer
    Raymond R Tubbs
    JAMA 292:1817-8; author reply 1818. 2004
  73. ncbi HER-2 amplification in tubular carcinoma of the breast
    Gerard J Oakley
    Marshall University School of Medicine, Huntington, WV, USA
    Am J Clin Pathol 126:55-8. 2006
    ..01). HER-2 gene amplification likely occurs infrequently, or not at all, in tubular carcinomas of the breast, whereas most express estrogen receptors...
  74. ncbi Role of HPV DNA testing in predicting cervical intraepithelial lesions: comparison of HC HPV and ISH HPV
    M Nasar Qureshi
    Department of Pathology, Bayonne Medical Center, Bayonne, New Jersey, USA
    Diagn Cytopathol 29:149-55. 2003
    ....
  75. ncbi Interlaboratory comparison of immunohistochemical testing for HER2: results of the 2004 and 2005 College of American Pathologists HER2 Immunohistochemistry Tissue Microarray Survey
    Patrick L Fitzgibbons
    Department of Pathology, St Jude Medical Center, Fullerton, Calif, USA
    Arch Pathol Lab Med 130:1440-5. 2006
    ..Cores found to be IHC-positive or IHC-negative by participant consensus can be used as validated benchmarks for interlaboratory comparison, allowing laboratories to assess their performance and determine if improvements are needed...
  76. ncbi Metallographic in situ hybridization
    Richard D Powell
    Nanoprobes, Incorporated, 95 Horseblock Road, Unit 1, Yaphank, NY 11980, USA
    Hum Pathol 38:1145-59. 2007
    ..Metallographic staining also provides excellent results for immunohistochemistry and may be combined with other staining procedures for the simultaneous detection of more than one gene or combinations of genes and proteins...
  77. ncbi A new rabbit monoclonal antibody (4B5) for the immunohistochemical (IHC) determination of the HER2 status in breast cancer: comparison with CB11, fluorescence in situ hybridization (FISH), and interlaboratory reproducibility
    William C Powell
    Ventana Medical Systems Inc, Tucson, AZ, USA
    Appl Immunohistochem Mol Morphol 15:94-102. 2007
    ..3% SI) in both cohorts. Interlaboratory reproducibility was also excellent (kappa 1.0). RMoAb 4B5 provides excellent sensitivity, specificity, and interlaboratory reproducibility for the detection of HER2 status in breast cancer...
  78. ncbi The CD117 immunohistochemistry tissue microarray survey for quality assurance and interlaboratory comparison: a College of American Pathologists Cell Markers Committee Study
    David M Dorfman
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Arch Pathol Lab Med 130:779-82. 2006
    ..Consequently, correct identification of c-Kit expression is important for the diagnosis and treatment of GISTs...
  79. ncbi Dual-color, break-apart fluorescence in situ hybridization for EWS gene rearrangement distinguishes clear cell sarcoma of soft tissue from malignant melanoma
    Rajiv M Patel
    Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30355, USA
    Mod Pathol 18:1585-90. 2005
    ..As the studied probe can be utilized in routinely processed tissue, FISH provides an excellent alternative to reverse-transcriptase polymerase chain reaction in cases where fresh tissue is unavailable...
  80. ncbi Detection of clonal IGH gene rearrangements: summary of molecular oncology surveys of the College of American Pathologists
    Marina N Nikiforova
    Department of Pathology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    Arch Pathol Lab Med 131:185-9. 2007
    ....
  81. ncbi Tissue microarrays: robust molecular morphology tools
    Lawrence E DeBault
    J Mol Histol 38:111-2. 2007
  82. ncbi Molecular pathology testing of tissues fixed in prefer solution
    Raymond R Tubbs
    Am J Surg Pathol 28:417-9. 2004
  83. ncbi Clinical laboratory reports in molecular pathology
    Margaret L Gulley
    Department of Pathology, 913 Brinkhous Bullitt Bldg, University of North Carolina, Chapel Hill, NC 27599 7525, USA
    Arch Pathol Lab Med 131:852-63. 2007
    ..The recent introduction of array technology has added another layer of complexity involving massive parallel analysis of multiple genes, transcripts, or proteins...
  84. ncbi In vivo recombinant adenovirus-mediated p53 gene therapy in a syngeneic rat model for colorectal cancer
    Jeong-Heum Baek
    Department of Surgery, Gachon Medical School, Gil Medical Center, Incheon, Korea
    J Korean Med Sci 19:834-41. 2004
    ..These results demonstrate that gene replacement therapy with p53 may provide a novel modality of treatment in conjunction with other present treatments for metastatic colorectal cancer...