Research Topics
| Jean F WelterSummaryAffiliation: Case Western Reserve University Country: USA Publications
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Detail Information
Publications
Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assayJean F Welter
Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA
Biotechniques 42:732, 734-7. 2007..Thus, it appears safe to eliminate the centrifugation step from the aggregate culture protocol. This results in significant time and effort savings and paves the way for future full automation of the aggregate assay...- Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cellsLuis A Solchaga
Division of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7080, USA
Tissue Eng Part A 16:1009-19. 2010..Supplementation with FGF-2 allows reaching target cell numbers more rapidly and extends the level of expansion within which these cells are useful for tissue-engineered cartilage repair... - FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cellsLuis A Solchaga
Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
J Cell Physiol 203:398-409. 2005..With the generation of gene expression profiles of FGF-treated and control cells we have taken the first steps in the elucidation of the molecular mechanism(s) behind these phenomena... - Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytesReid Wenger
Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106-4946, USA
Front Biosci 11:1690-5. 2006..02) the proportion of apoptotic cells in the cartilage-constructs (41% +/- 4.2%; mean +/- SD) compared to control cartilage-constructs (28.5 +/- 8.4%)... - Nondestructive evaluation of hydrogel mechanical properties using ultrasoundJason M Walker
Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
Ann Biomed Eng 39:2521-30. 2011..A stronger correlation with mechanical measurements was found using the poroelastic rather than the elastic model. Results from this preliminary testing will be used to guide further studies of native and engineered cartilage... - High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cellsKitsie J Penick
Case Western Reserve University, Cleveland, OH 44106-7080, USA
Biotechniques 39:687-91. 2005..When analyzed histologically, both systems demonstrate morphological characteristics that are consistent with chondrogenic differentiation and cartilaginous extracellular matrix production... - Performance of polyoxymethylene plastic (POM) as a component of a tissue engineering bioreactorKitsie J Penick
Department of Biology (Skeletal Research Center, Case Western Reserve University, Cleveland, OH 44106, USA
J Biomed Mater Res A 75:168-74. 2005..The contrast of these findings to those of LaIuppa et al.5 may reflect a cell-type specific sensitivity, or may be due to different handling of the material... - Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricksLuis A Solchaga
Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
Methods Mol Biol 698:253-78. 2011.... - Concentrated collagen-chondroitin sulfate scaffolds for tissue engineering applicationsWan Hsiang Liang
Department of Chemical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Biomed Mater Res A 94:1050-60. 2010..In addition, our tissue culture results with human mesenchymal stem cells and foreskin keratinocytes show that the new scaffolds can be used for cartilage and skin tissue-engineering applications... - Cartilage tissue engineering for laryngotracheal reconstruction: comparison of chondrocytes from three anatomic locations in the rabbitJames H Henderson
Departments of Biology and Orthopaedics, Skeletal Research Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
Tissue Eng 13:843-53. 2007..The results also suggest that this and similar tissue engineering approaches must be optimized for each potential source of chondrocytes... - Primary cilia modulate Ihh signal transduction in response to hydrostatic loading of growth plate chondrocytesYvonne Y Shao
Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH 44195, USA
Bone 50:79-84. 2012..These results suggest that growth plate chondrocytes respond to hydrostatic loading by increasing Ihh signaling, and that the primary cilium is required for this mechano-biological signal transduction to occur... - Evaluation of machining methods for trabecular metal implants in a rabbit intramedullary osseointegration modelMukund Deglurkar
Department of Orthopaedics, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Biomed Mater Res B Appl Biomater 80:528-40. 2007..The resulting mechanical properties of the bone implant-interface were similar in all three groups, with only interface stiffness and interface shear modulus being significantly higher in the machined samples... - High-efficiency nonviral transfection of primary chondrocytesJean F Welter
Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
Methods Mol Med 100:129-46. 2004..In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE 6 transfection... - Hydrostatic pressure induces apoptosis in human chondrocytes from osteoarthritic cartilage through up-regulation of tumor necrosis factor-alpha, inducible nitric oxide synthase, p53, c-myc, and bax-alpha, and suppression of bcl-2Najmul Islam
Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4946, USA
J Cell Biochem 87:266-78. 2002..These results indicated that HP is a strong inducer of apoptosis in osteoarthritic human chondrocytes in vitro... - Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotypeLuis A Solchaga
Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
Methods Mol Med 100:53-68. 2004..Although we have the technology to accomplish chondrogenic differentiation of stem cells, much is still to be learned regarding the regulatory mechanisms controlling the lineage transitions and maturation of the cartilaginous tissue... - Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive PotentialJeffery J Auletta
Divisions of Pediatric Hematology Oncology and Pediatric Infectious Diseases, Department of Pediatrics, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
Stem Cells Int 2011:235176. 2011..However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion... - Combined experimental and mathematical approach for development of microfabrication-based cancer migration assaySaheli Sarkar
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
Ann Biomed Eng 39:2346-59. 2011..The combined experimental and mathematical model can be used to quantitatively study the impact of cell-cell interactions on migration... - Sequence, gene structure, and expression pattern of CTNNBL1, a minor-class intron-containing gene--evidence for a role in apoptosisLeila Jabbour
Department of Anatomy, Case Western Reserve University, Cleveland, OH 44106, USA
Genomics 81:292-303. 2003..The rate of cell death was higher when cells were transfected with a carboxy-terminal fragment of CTNNBL1, suggesting that the apoptosis-inducing activity is a function of this region... - A rapid seeding technique for the assembly of large cell/scaffold composite constructsLuis A Solchaga
Department of Orthopaedics, Case Western Reserve University Cleveland, Ohio 44106-7080, USA
Tissue Eng 12:1851-63. 2006..The vacuum seeding technique is safe, rapid, reproducible, and results in controlled quantitative cell loading, high retention, and uniform distribution...