Chunfa Huang

Summary

Affiliation: Case Western Reserve University
Location: Cleveland, USA
Summary:
Fields: cell signal transduction
Expertise: Renal cell signaling, Cancer cell signaling, G protein-coupled receptor, regulator of G protein signal

Publications

  1. ncbi Phospholipase D activity in nontransformed and transformed fibroblasts
    C Huang
    Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, NC 27157 1016
    Biochim Biophys Acta 1127:242-8. 1992
  2. ncbi Interaction of the calcium-sensing receptor and filamin, a potential scaffolding protein
    H Awata
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, Cleveland, Ohio 44106, USA
    J Biol Chem 276:34871-9. 2001
  3. ncbi Functional characterization of a calcium-sensing receptor mutation in severe autosomal dominant hypocalcemia with a Bartter-like syndrome
    Rosa Vargas-Poussou
    INSERM U423, Department of Physiology, Universite Rene Descartes, Hopital Necker Enfants Malades, Paris, France
    J Am Soc Nephrol 13:2259-66. 2002
  4. ncbi Differentially regulated expression of endogenous RGS4 and RGS7
    Andrejs M Krumins
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
    J Biol Chem 279:2593-9. 2004
  5. ncbi Mechanical force-activated phospholipase D is mediated by Galpha12/13-Rho and calmodulin-dependent kinase in renal epithelial cells
    Jenny Ziembicki
    Dept. of Medicine, Case Western Reserve Univ, Louis Stokes Veterans Affairs Medical Ctr, 10701 East Blvd, 151W, Cleveland, OH 44106, USA
    Am J Physiol Renal Physiol 289:F826-34. 2005
  6. ncbi Fibrocystin interacts with CAML, a protein involved in Ca2+ signaling
    Junko Nagano
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Lois Stokes Veterans Affairs Medical Center, Cleveland, OH, USA
    Biochem Biophys Res Commun 338:880-9. 2005
  7. ncbi HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties
    R Tandon
    Department of Surgery, Case Western Reserve University, Cleveland, OH 44106, USA
    Am J Physiol Renal Physiol 292:F701-10. 2007
  8. ncbi Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function
    Chunfa Huang
    Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Am J Physiol Renal Physiol 292:F1073-81. 2007
  9. ncbi MUPP1 complexes renal K+ channels to alter cell surface expression and whole cell currents
    Aleksandra Sindic
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Am J Physiol Renal Physiol 297:F36-45. 2009
  10. ncbi Calcium-sensing receptor decreases cell surface expression of the inwardly rectifying K+ channel Kir4.1
    Seung Kuy Cha
    Department of Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 286:1828-35. 2011

Collaborators

  • R Tyler Miller
  • S M Mumby
  • Michael F Romero
  • P A Janmey
  • L A Bruggeman
  • Ceredwyn E Hill
  • Stephen J Gold
  • Kim W Chan
  • Rosa Vargas Poussou
  • P Houillier
  • Yaxian Ding
  • M E Handlogten
  • Jenny Ziembicki
  • Junko Nagano
  • H Awata
  • Hans M Wyss
  • Seung Kuy Cha
  • Aleksandra Sindic
  • R Tandon
  • Kristine M Hujer
  • Rajnish Tandon
  • Zhenzhen Wu
  • Anoop Kumar Padival
  • Andrejs M Krumins
  • Rosa Vargas-Poussou
  • Joel M Henderson
  • David A Weitz
  • Thomas Franke
  • Elisa Mele
  • Xiaoping Qi
  • Martin R Pollak
  • N Shiraishi
  • Jeffrey H Miner
  • Fitzroy J Byfield
  • Jung Hee Suh
  • Danian Che
  • William A Miller-Little
  • An Ping Chen
  • J R Sedor
  • F J Byfield
  • J R Schelling
  • I Levental
  • J Ziembicki
  • Richard J Bram
  • Ulrich Hopfer
  • John R Sedor
  • Jeffrey R Schelling
  • Kimio Tomita
  • Kenichiro Kitamura
  • Christopher J Ward
  • Karen S Hawkins
  • Kan Yu
  • Thomas M Wilkie
  • Roger K Sunahara
  • Sheryll A Barker
  • Corinne Antignac
  • Gabrielle Planelles
  • Philippe Hulin
  • Xavier Jeunemaitre
  • Michele Dechaux
  • Michel Paillard
  • L W Daniel
  • R L Wykle
  • D Greene
  • J C Strum
  • P K Smitherman

Detail Information

Publications32

  1. ncbi Phospholipase D activity in nontransformed and transformed fibroblasts
    C Huang
    Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, NC 27157 1016
    Biochim Biophys Acta 1127:242-8. 1992
    ..The results from cell-free assays suggest that PLD activity is more dependent on the type of PC substrate than on the source of the enzyme...
  2. ncbi Interaction of the calcium-sensing receptor and filamin, a potential scaffolding protein
    H Awata
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, Cleveland, Ohio 44106, USA
    J Biol Chem 276:34871-9. 2001
    ....
  3. ncbi Functional characterization of a calcium-sensing receptor mutation in severe autosomal dominant hypocalcemia with a Bartter-like syndrome
    Rosa Vargas-Poussou
    INSERM U423, Department of Physiology, Universite Rene Descartes, Hopital Necker Enfants Malades, Paris, France
    J Am Soc Nephrol 13:2259-66. 2002
    ....
  4. ncbi Differentially regulated expression of endogenous RGS4 and RGS7
    Andrejs M Krumins
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
    J Biol Chem 279:2593-9. 2004
    ..Although we searched without finding evidence for regulation of RGS4 proteolysis, the possibility remains that alterations in the degradation of this protein could provide a means to promptly alter patterns of signal transduction...
  5. ncbi Mechanical force-activated phospholipase D is mediated by Galpha12/13-Rho and calmodulin-dependent kinase in renal epithelial cells
    Jenny Ziembicki
    Dept. of Medicine, Case Western Reserve Univ, Louis Stokes Veterans Affairs Medical Ctr, 10701 East Blvd, 151W, Cleveland, OH 44106, USA
    Am J Physiol Renal Physiol 289:F826-34. 2005
    ..The signaling pathways for PLD activation involve Galpha(12/13)/Rho/F-actin and calmodulin-dependent kinase...
  6. ncbi Fibrocystin interacts with CAML, a protein involved in Ca2+ signaling
    Junko Nagano
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Lois Stokes Veterans Affairs Medical Center, Cleveland, OH, USA
    Biochem Biophys Res Commun 338:880-9. 2005
    ..Fibrocystin may participate in regulation of intracellular Ca(2+) in the distal nephron in a manner similar to PKD1 and PKD2 that are involved in autosomal dominant polycystic kidney disease...
  7. ncbi HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties
    R Tandon
    Department of Surgery, Case Western Reserve University, Cleveland, OH 44106, USA
    Am J Physiol Renal Physiol 292:F701-10. 2007
    ..The mechanical properties of the WT podocytes suggest that these cells can better maintain capillary integrity than the HIVAN podocytes and implicate pathological assembly of the cytoskeleton as a mechanism of HIVAN...
  8. ncbi Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function
    Chunfa Huang
    Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Am J Physiol Renal Physiol 292:F1073-81. 2007
    ..The CaR interacts directly with Kir4.1 and Kir4.2 and can decrease their currents, which in turn could reduce recycling of K(+) for the basolateral Na(+)-K(+)-ATPase and thereby contribute to inhibition of Na(+) reabsorption...
  9. ncbi MUPP1 complexes renal K+ channels to alter cell surface expression and whole cell currents
    Aleksandra Sindic
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Am J Physiol Renal Physiol 297:F36-45. 2009
    ..2 interacts selectively with MUPP1 to affect its cell surface expression. Thus MUPP1 and Kir4.2 may participate in a protein complex in the nephron that could regulate transport of K(+) as well as other ions...
  10. ncbi Calcium-sensing receptor decreases cell surface expression of the inwardly rectifying K+ channel Kir4.1
    Seung Kuy Cha
    Department of Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 286:1828-35. 2011
    ..Thus, the CaR decreases cell surface expression of Kir4.1 channels via a mechanism that involves G?(q) and caveolin. These results provide a novel molecular basis for the inhibition of renal NaCl transport by the CaR...
  11. ncbi The Ca2+-sensing receptor activates cytosolic phospholipase A2 via a Gqalpha -dependent ERK-independent pathway
    M E Handlogten
    Division of Nephrology, Department of Medicine, University of Florida, Gainesville, Florida 32610, USA
    J Biol Chem 276:13941-8. 2001
    ..These results demonstrate that the CaR activates cPLA(2) via a Galpha(q), PLC, Ca(2+)-CaM, and calmodulin-dependent protein kinase-dependent pathway that is independent the ERK pathway...
  12. ncbi Extracellular Ca(2+)-sensing receptor is a promiscuous divalent cation sensor that responds to lead
    M E Handlogten
    Division of Nephrology, Department of Medicine, University of Florida, Gainesville, Florida 32610, USA
    Am J Physiol Renal Physiol 279:F1083-91. 2000
    ....
  13. ncbi Comparison of phospholipase D activity in vasopressin- and phorbol ester-stimulated fibroblasts
    C Huang
    Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, NC 27157 1016
    FEBS Lett 319:141-4. 1993
    ..The data suggest that REF52 cells contain dual PLD activities. The first is stimulated only by VP, requires Ca2+ and hydrolyzes PI. The second is stimulated by both TPA and VP, activated by protein kinase C and hydrolyzes PC...
  14. ncbi Phospholipase D hydrolyzes ether- and ester-linked glycerophospholipids by different pathways in MDCK cells
    C Huang
    Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, NC 27157 1016, USA
    Biochem Biophys Res Commun 213:950-7. 1995
    ..These results suggest that the selective hydrolysis of PLD action may play an important role in cellular signal transduction under physiological and pathological conditions...
  15. ncbi Phorbol diesters stimulate the accumulation of phosphatidate, phosphatidylethanol, and diacylglycerol in three cell types. Evidence for the indirect formation of phosphatidylcholine-derived diacylglycerol by a phospholipase D pathway and direct formation
    C F Huang
    W Alton Jones Cell Science Center, Lake Placid, New York 12946
    J Biol Chem 265:14858-63. 1990
    ..These results show that TPA elicits DG formation from PC in MDCK cells predominantly by an indirect pathway, whereas in arterial smooth muscle cells DG is formed in part by the direct action of PLC.(ABSTRACT TRUNCATED AT 400 WORDS)..
  16. ncbi Attenuation of Gi- and Gq-mediated signaling by expression of RGS4 or GAIP in mammalian cells
    C Huang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA
    Proc Natl Acad Sci U S A 94:6159-63. 1997
    ..RGS4 and GAIP both attenuated Gi-mediated inhibition of cAMP synthesis. RGS4 was more effective than GAIP in blocking Gq-mediated activation of phospholipase Cbeta...
  17. ncbi Vasopressin-induced polyphosphoinositide and phosphatidylcholine degradation in fibroblasts. Temporal relationship for formation of phospholipase C and phospholipase D hydrolysis products
    C F Huang
    W Alton Jones Cell Science Center, Inc, Lake Placid, New York 12946
    J Biol Chem 265:17468-73. 1990
    ..From the time courses shown, we hypothesize that the early generation of PA, heretofore ascribed to products of the polyphosphoinositide cycle, are in part derived from PC by phospholipase D...
  18. ncbi Organization of G proteins and adenylyl cyclase at the plasma membrane
    C Huang
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235 9041, USA
    Mol Biol Cell 8:2365-78. 1997
    ..The data suggest that hormone-sensitive adenylyl cyclase systems are localized in a specialized subdomain of the plasma membrane that may optimize the efficiency and fidelity of signal transduction...
  19. ncbi Identification of phosphatidylcholine-selective and phosphatidylinositol-selective phospholipases D in Madin-Darby canine kidney cells
    C Huang
    Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, North Carolina 27157 1016
    J Biol Chem 267:16859-65. 1992
    ....
  20. ncbi Persistent membrane association of activated and depalmitoylated G protein alpha subunits
    C Huang
    University of Texas Southwestern Medical Center, Department of Pharmacology, 5323 Harry Hines Boulevard, Dallas, TX 75235 9041, USA
    Proc Natl Acad Sci U S A 96:412-7. 1999
    ..Limitation of alpha subunits to the plasma membrane obviously restricts their mobility and may contribute to the efficiency and specificity of signaling...
  21. ncbi Phospholipase D hydrolysis of choline phosphoglycerides is selective for the alkyl-linked subclass of Madin-Darby canine kidney cells
    L W Daniel
    Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston Salem, North Carolina 27157
    J Biol Chem 268:21519-26. 1993
    ..In summary, TPA-stimulated phospholipase D is selective for the alkyl-PC subclass in MDCK cells. The alkyl-DG subsequently formed does not appear to function as a second-messenger in activating protein kinase C...
  22. ncbi Biophysical properties of normal and diseased renal glomeruli
    Hans M Wyss
    Physics, Harvard University, Cambridge, Massachusetts, USA
    Am J Physiol Cell Physiol 300:C397-405. 2011
    ..This increased deformability of glomeruli could directly contribute to disease by permitting increased distension with hemodynamic force or represent a mechanically inhospitable environment for glomerular cells...
  23. ncbi Role of ceramide in Ca2+-sensing receptor-induced apoptosis
    Zhenzhen Wu
    Department of Medicine, Case Western Reserve University, Cleveland, OH, USA
    J Lipid Res 46:1396-404. 2005
    ....
  24. ncbi Parallel activation of phosphatidylinositol 4-kinase and phospholipase C by the extracellular calcium-sensing receptor
    Chunfa Huang
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, Cleveland, Ohio 44106, USA
    J Biol Chem 277:20293-300. 2002
    ..Taken together, our data demonstrate that CaR stimulates PI 4-kinase, the first step in inositol lipid biosynthesis conversion of PI to PI 4-P by Rho-dependent and Galpha(q)- and Galpha(i)-independent pathways...
  25. ncbi The calcium-sensing receptor and its interacting proteins
    Chunfa Huang
    Department of Medicine and Physiology, Case Western Reserve University, Louis Stokes VAMC Rammelkamp Center for Research, Metro Health Medical Center, Cleveland, Ohio, USA
    J Cell Mol Med 11:923-34. 2007
    ..These and other proteins may not all be associated with the CaR in all tissues, but they form the basis for understanding the complete nature of CaR signalling...
  26. ncbi The Ca2+-sensing receptor couples to Galpha12/13 to activate phospholipase D in Madin-Darby canine kidney cells
    Chunfa Huang
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, 10701 East Boulevard 151W, Cleveland, OH 44106, USA
    Am J Physiol Cell Physiol 286:C22-30. 2004
    ..This suggests that the CaR may regulate cytoskeleton via Galpha12/13, Rho, and PLD...
  27. ncbi Novel Ca receptor signaling pathways for control of renal ion transport
    Chunfa Huang
    Departments of Medicine and Physiology, Case Western Reserve University, Louis Stokes VAMC and Rammelkamp Center for Research, MetroHealth Medical Center, Cleveland, Ohio, USA
    Curr Opin Nephrol Hypertens 19:106-12. 2010
    ..This review will summarize recent information regarding the renal transport effects of the CaR...
  28. ncbi Regulation of renal ion transport by the calcium-sensing receptor: an update
    Chunfa Huang
    Department of Medicine, Case Western Reserve University, Louis Stokes VAMC, Rammelkamp Center for Research, MetroHealth Medical Center, Cleveland, Ohio, USA
    Curr Opin Nephrol Hypertens 16:437-43. 2007
    ....
  29. ncbi Role of p115RhoGEF in the regulation of extracellular Ca(2+)-induced choline kinase activation and prostate cancer cell proliferation
    Chunfa Huang
    Louis Stokes Cleveland Veteran Affairs Medical Center, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Int J Cancer 128:2833-42. 2011
    ..The results demonstrate an important role of p115RhoGEF in prostate tumorigenesis and provide a potential target of cancer therapeutics...
  30. ncbi Activation of choline kinase by extracellular Ca2+ is Ca(2+)-sensing receptor, Galpha12 and Rho-dependent in breast cancer cells
    Chunfa Huang
    Louis Stokes Cleveland Veteran Affairs Medical Center, Cleveland, Ohio 44106, United States
    Cell Signal 21:1894-900. 2009
    ....
  31. ncbi Silencing of filamin A gene expression inhibits Ca2+ -sensing receptor signaling
    Chunfa Huang
    Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, 10701 East Blvd, 151W, Cleveland, OH 44106, USA
    FEBS Lett 580:1795-800. 2006
    ..This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function...
  32. ncbi High glucose-induced membrane translocation of PKC betaI is associated with Arf6 in glomerular mesangial cells
    Anoop Kumar Padival
    Department of Medicine, Case Western Reserve University, Cleveland, OH, USA
    Mol Cell Biochem 258:129-35. 2004
    ....