Michael L Metzker

Summary

Affiliation: Baylor College of Medicine
Country: USA

Publications

  1. ncbi Emerging technologies in DNA sequencing
    Michael L Metzker
    Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genome Res 15:1767-76. 2005
  2. ncbi Glass bead purification of plasmid template DNA for high throughput sequencing of mammalian genomes
    Debra A Dederich
    Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, 1 Baylor Plaza, ALKEK Building, N1409, Houston, TX 77030, USA
    Nucleic Acids Res 30:e32. 2002
  3. ncbi Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates
    Weidong Wu
    LaserGen, Inc, Houston, TX 77054, USA
    Nucleic Acids Res 35:6339-49. 2007
  4. ncbi Molecular evidence of HIV-1 transmission in a criminal case
    Michael L Metzker
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:14292-7. 2002
  5. ncbi The DNA sequence, annotation and analysis of human chromosome 3
    Donna M Muzny
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 440:1194-8. 2006
  6. ncbi The finished DNA sequence of human chromosome 12
    Steven E Scherer
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 440:346-51. 2006
  7. ncbi Improved nucleotide selectivity and termination of 3'-OH unblocked reversible terminators by molecular tuning of 2-nitrobenzyl alkylated HOMedU triphosphates
    VLADISLAV A LITOSH
    LaserGen, Inc, Houston, TX 77054, USA
    Nucleic Acids Res 39:e39. 2011
  8. ncbi Sequencing technologies - the next generation
    Michael L Metzker
    Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Rev Genet 11:31-46. 2010
  9. ncbi Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution
    Stephen Richards
    Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston Texas 77030, USA
    Genome Res 15:1-18. 2005
  10. ncbi Source identification in two criminal cases using phylogenetic analysis of HIV-1 DNA sequences
    Diane I Scaduto
    Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:21242-7. 2010

Research Grants

  1. Development of Fluorescent Detector for DNA Sequencing
    Michael Metzker; Fiscal Year: 2004
  2. Screening Tag Pol I Variants using 3'-O- Modified-dNTPs
    Michael Metzker; Fiscal Year: 2004
  3. Development of a portable PME DNA sequencer
    Michael Metzker; Fiscal Year: 2005

Detail Information

Publications15

  1. ncbi Emerging technologies in DNA sequencing
    Michael L Metzker
    Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genome Res 15:1767-76. 2005
    ....
  2. ncbi Glass bead purification of plasmid template DNA for high throughput sequencing of mammalian genomes
    Debra A Dederich
    Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, 1 Baylor Plaza, ALKEK Building, N1409, Houston, TX 77030, USA
    Nucleic Acids Res 30:e32. 2002
    ..2% and 516 bases, respectively. The cost of this direct lysis/glass bead method in September 2001 was approximately 10 cents per clone, which is a significant cost saving in high throughput genomic sequencing efforts...
  3. ncbi Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates
    Weidong Wu
    LaserGen, Inc, Houston, TX 77054, USA
    Nucleic Acids Res 35:6339-49. 2007
    ..A five-base experiment, illustrating the exquisite, stepwise addition through a homopolymer repeat, demonstrates the applicability of the N(6)-(2-nitrobenzyl)-dATP as an ideal RT for CRT sequencing...
  4. ncbi Molecular evidence of HIV-1 transmission in a criminal case
    Michael L Metzker
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:14292-7. 2002
    ..Resampling of blood from the suspected transmission pair and independent sequencing by different laboratories provided precaution against laboratory error...
  5. ncbi The DNA sequence, annotation and analysis of human chromosome 3
    Donna M Muzny
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 440:1194-8. 2006
    ....
  6. ncbi The finished DNA sequence of human chromosome 12
    Steven E Scherer
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 440:346-51. 2006
    ..The rate of base substitutions in recent evolutionary history shows an overall slowing in hominids compared with primates and rodents...
  7. ncbi Improved nucleotide selectivity and termination of 3'-OH unblocked reversible terminators by molecular tuning of 2-nitrobenzyl alkylated HOMedU triphosphates
    VLADISLAV A LITOSH
    LaserGen, Inc, Houston, TX 77054, USA
    Nucleic Acids Res 39:e39. 2011
    ..Modified nucleotides based on 5-hydroxymethyl-pyrimidines and 7-deaza-7-hydroxymethyl-purines lay the foundation for development of a complete set of four reversible terminators for application in NGS technologies...
  8. ncbi Sequencing technologies - the next generation
    Michael L Metzker
    Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Rev Genet 11:31-46. 2010
    ..I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest...
  9. ncbi Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution
    Stephen Richards
    Human Genome Sequencing Center and Department of Molecular and Human Genetics, Baylor College of Medicine, Houston Texas 77030, USA
    Genome Res 15:1-18. 2005
    ..Overall, a pattern of repeat-mediated chromosomal rearrangement, and high coadaptation of both male genes and cis-regulatory sequences emerges as important themes of genome divergence between these species of Drosophila...
  10. ncbi Source identification in two criminal cases using phylogenetic analysis of HIV-1 DNA sequences
    Diane I Scaduto
    Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:21242-7. 2010
    ..The observed paraphyly from each case study led to the identification of an inferred source (i.e., index case), whose identity was revealed at trial to be that of the defendant...
  11. ncbi Genome sequence of the Brown Norway rat yields insights into mammalian evolution
    Richard A Gibbs
    Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, MS BCM226, One Baylor Plaza, Houston, Texas 77030, USA <http://www.hgsc.bcm.tmc.edu
    Nature 428:493-521. 2004
    ....
  12. ncbi Color-blind fluorescence detection for four-color DNA sequencing
    Ernest K Lewis
    Human Genome Sequencing Center, Department of Molecular and Human Genetics, Cell and Molecular Biology Program, Baylor College of Medicine, Houston, TX 77030
    Proc Natl Acad Sci U S A 102:5346-51. 2005
    ..These advantages translate into significantly enhanced signal quality as illustrated in the primary DNA sequencing data and provide a means for achieving accurate base-calling at lower reagent concentrations...
  13. ncbi The DNA sequence of the human X chromosome
    Mark T Ross
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 434:325-37. 2005
    ..Of this number, 168 have been explained by mutations in 113 X-linked genes, which in many cases were characterized with the aid of the DNA sequence...
  14. ncbi Rigid, conjugated, fluoresceinated thymidine triphosphates: syntheses and polymerase mediated incorporation into DNA analogues
    Lars H Thoresen
    Texas A and M University, Chemistry Department, P. O. Box 30012, College Station, TX 77842, USA
    Chemistry 9:4603-10. 2003
    ..The 2',3'-dideoxy nucleoside 3 b was incorporated only about 430 times less efficiently than ddTTP under the same conditions, and caused chain termination. The implications of these studies on modified sequencing protocols are discussed...
  15. ncbi Syntheses, photophysical properties, and application of through-bond energy-transfer cassettes for biotechnology
    Guan-Sheng Jiao
    Department of Chemistry, Texas A and M University P.O. Box 30012, College Station, Texas 77842, USA
    Chemistry 12:7816-26. 2006
    ..Modification of a commercial automated DNA-sequencing apparatus to detect the emissions of these four energy-transfer cassettes enabled single-color dye-primer sequencing...

Research Grants4

  1. Development of Fluorescent Detector for DNA Sequencing
    Michael Metzker; Fiscal Year: 2004
    ..abstract_text> ..
  2. Screening Tag Pol I Variants using 3'-O- Modified-dNTPs
    Michael Metzker; Fiscal Year: 2004
    ..Successful implementation of these aims will result would demonstrate the BASS technology for application of de novo SNP discovery. ..
  3. Development of a portable PME DNA sequencer
    Michael Metzker; Fiscal Year: 2005
    ..It is anticipated that microPME instruments will have broad applications for routine usage for clinical diagnosis, forensic analyses, military applications, and general sequencing purposes. ..