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| Erica SpackmanSummaryAffiliation: Agricultural Research Service Country: USA Publications
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Publications
An avian influenza virus from waterfowl in South America contains genes from North American avian and equine lineagesErica Spackman
Southeast Poultry Research Laboratory, U S Department of Agriculture Agricultural Research Service, 934 College Station Road, Athens, GA 30605, USA
Avian Dis 51:273-4. 2007..Two genes were most closely related to North American wild aquatic bird virus lineages and one gene was most closely related to an equine influenza virus from South America...
H7N3 avian influenza virus found in a South American wild duck is related to the Chilean 2002 poultry outbreak, contains genes from equine and North American wild bird lineages, and is adapted to domestic turkeysErica Spackman
Southeast Poultry Research Laboratory, US Department of Agriculture ARS, 934 College Station Road, Athens, GA 30605, USA
J Virol 80:7760-4. 2006..The HA protein cleavage site and the results of pathogenesis studies in chickens were consistent with a low-pathogenicity virus, and the infective dose was 10(5) times higher for chickens than turkeys...
The pathogenesis of turkey origin reoviruses in turkeys and chickensErica Spackman
Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Road, Athens, GA 30605, USA
Avian Pathol 34:291-6. 2005..Molecular characterization of the isolates revealed that the TRVs and chicken origin reoviruses had identical electropherotype profiles...
Phylogenetic analyses of type A influenza genes in natural reservoir species in North America reveals genetic variationErica Spackman
Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Road, Athens, GA 30605, USA
Virus Res 114:89-100. 2005..A lack of clear temporal or spatial grouping shows that multiple lineages of virus co-circulate and precludes the development of an epidemiological map for influenza virus from these species...
Characterization of highly pathogenic H5N1 avian influenza A viruses isolated from South KoreaChang-Won Lee
Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd, Athens, GA 30605, USA
J Virol 79:3692-702. 2005....
Characterization of low-pathogenicity H5N1 avian influenza viruses from North AmericaErica Spackman
Southeast Poultry Research Laboratory, U S Department of Agriculture Agricultural Research Service, 934 College Station Road, Athens, GA 30605, USA
J Virol 81:11612-9. 2007....
Characterization of recent H5 subtype avian influenza viruses from US poultryChang-Won Lee
Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Road, Athens, GA 30605, USA
Avian Pathol 33:288-97. 2004..The outbreak in Texas with a more chicken-adapted H5N3 virus underscores the importance of ongoing surveillance and control efforts regarding the H5 subtype AI virus in the US...
Characterization and efficacy determination of commercially available Central American H5N2 avian influenza vaccines for poultryDawn Eggert
Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA
Vaccine 28:4609-15. 2010....
Enteric viruses detected by molecular methods in commercial chicken and turkey flocks in the United States between 2005 and 2006Mary J Pantin-Jackwood
Southeast Poultry Research Laboratory, USDA, Agricultural Research Service, Athens, GA 30605, USA
Avian Dis 52:235-44. 2008....
Removal of real-time reverse transcription polymerase chain reaction (RT-PCR) inhibitors associated with cloacal swab samples and tissues for improved diagnosis of Avian influenza virus by RT-PCRAmaresh Das
Southeast Poultry Research Laboratory, Agricultural Research Service, USDA, Athens, GA 30605, USA
J Vet Diagn Invest 21:771-8. 2009..The MagMAX-M protocol reported in the present study can be useful in extracting high-quality RNA for accurate detection of AIV from cloacal swabs and tissues by real-time RT-PCR...
Biologic characterization of H4, H6, and H9 type low pathogenicity avian influenza viruses from wild birds in chickens and turkeysAntonio C Morales
Department of Population Health, College of Veterinary Medicine, University of Georgia, 953 College Station Road, Athens, GA 30602, USA
Avian Dis 53:552-62. 2009..Specifically, detection is difficult because these viruses did not cause overt clinical disease or mortality, but only induced mild microscopic lesions and exhibited poor seroconversion...
Characterization of low pathogenicity avian influenza viruses isolated from wild birds in Mongolia 2005 through 2007Erica Spackman
U S Department of Agriculture, Agricultural Research Service, Southeast Poultry Research Laboratory, Athens, GA 30605, USA
Virol J 6:190. 2009..An important feature of Mongolia is that there is little domestic poultry production in the country, therefore AIV detection in wild birds would not likely be from spill-over from domestic poultry...
The pathogenesis of low pathogenicity H7 avian influenza viruses in chickens, ducks and turkeysErica Spackman
Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Rd, Athens, GA, 30605, USA
Virol J 7:331. 2010..In this study twelve H7 LPAI virus isolates from North America were each evaluated for their comparative pathogenesis in chickens, ducks, and turkeys...
The pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducksEnid T McKinley
Southeast Poultry Research Laboratory, USDA, Agricultural Research Service, Athens, GA 30605, USA
Influenza Other Respi Viruses 4:353-6. 2010....
Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reovirusesJ Michael Day
Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Road, Athens, GA 30605, USA
Virus Genes 35:235-42. 2007..This report supports the earlier suggestion that the TRVs are a separate virus species within the Orthoreovirus genus, and may provide some insight into TRV host specificity and pathogenesis...
Analytical validation of a real-time reverse transcription polymerase chain reaction test for Pan-American lineage H7 subtype Avian influenza virusesErica Spackman
Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, GA 30605, USA
J Vet Diagn Invest 20:612-6. 2008..Furthermore, the 2008 test was able to detect 100% (n = 27) of the H7 AI, V isolates recovered from North American wild birds in a 2006-2007 sample set, (none of which were detected by the 2002 H7 test)...
NP, PB1, and PB2 viral genes contribute to altered replication of H5N1 avian influenza viruses in chickensJamie L Wasilenko
Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Road, Athens, GA 30605, USA
J Virol 82:4544-53. 2008..While the pathogenesis of AIVs in chickens is clearly dependent on the interaction of multiple gene products, we have shown that single-gene reassortment events are sufficient to alter the virulence of AIVs in chickens...
Sequence analysis of recent H7 avian influenza viruses associated with three different outbreaks in commercial poultry in the United StatesErica Spackman
Southeast Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA
J Virol 77:13399-402. 2003..Furthermore, analysis of the HA cleavage site amino acid sequence, a marker for pathogenicity in chickens and turkeys, shows a progression toward a cleavage site sequence that fulfills the molecular criteria for highly pathogenic AIV...
A multiplex RT-PCR test for the differential identification of turkey astrovirus type 1, turkey astrovirus type 2, chicken astrovirus, avian nephritis virus, and avian rotavirusJ Michael Day
Southeast Poultry Research Laboratory, USDA, ARS, 934 College Station Road, Athens, GA 30605, USA
Avian Dis 51:681-4. 2007..The specificity was further shown by testing 109 chicken specimens and 32 turkey specimens from commercial flocks with the appropriate test and sequencing the RT-PCR amplicons to confirm amplification of the correct target...
Turkey origin reovirus-induced immune dysfunction in specific pathogen free and commercial turkey poultsJ Michael Day
Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, Georgia 30605, USA
Avian Dis 52:387-91. 2008..This immune dysfunction and bursal atrophy was not present in commercial poults inoculated at three weeks of age...
Pathogenesis of type 2 turkey astroviruses with variant capsid genes in 2-day-old specific pathogen free poultsMary J Pantin-Jackwood
Southeast Poultry Research Laboratory, Agricultural Research Service, US Department of Agriculture, Athens, GA 30605, USA
Avian Pathol 37:193-201. 2008..In conclusion, TAstV-2 viruses with variant capsids produce a similar enteric disease in young turkeys and may also affect the immune system of the birds by causing bursal lymphoid depletion...
Astrovirus, reovirus, and rotavirus concomitant infection causes decreased weight gain in broad-breasted white poultsErica Spackman
Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA
Avian Dis 54:16-21. 2010..Cloacal shedding of TAstV-2 and TRotV was evaluated by reverse-transcription PCR testing of cloacal swabs and minimal differences were observed among the treatment groups...
Biologic characterization of chicken-derived H6N2 low pathogenic avian influenza viruses in chickens and ducksMark W Jackwood
Department of Population Health, College of Veterinary Medicine, University of Georgia, 953 College Station Road, Athens, GA 30602, USA
Avian Dis 54:120-5. 2010..However, the viruses isolated in 2004 did not appear to replicate or cause more disease than earlier virus isolates...
Phylogenetic analysis of Turkey astroviruses reveals evidence of recombinationMary J Pantin-Jackwood
Southeast Poultry Research Laboratory, Agriculture Research Service, US Department of Agriculture, Athens, GA 30605, USA
Virus Genes 32:187-92. 2006..Based on differing topologies of the capsid and polymerase gene phylogenetic trees, TAstV appears to undergo recombination...
Domestic pigs have low susceptibility to H5N1 highly pathogenic avian influenza virusesAleksandr S Lipatov
Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Athens, Georgia, United States of America
PLoS Pathog 4:e1000102. 2008....
Periodic monitoring of commercial turkeys for enteric viruses indicates continuous presence of astrovirus and rotavirus on the farmsMary J Pantin-Jackwood
Southeast Poultry Research Laboratory, Agricultural Research Service, U S Department of Agriculture, Athens, GA 30605, USA
Avian Dis 51:674-80. 2007..The full impact on flock performance needs to be further determined...
Development of an internal positive control for rapid diagnosis of avian influenza virus infections by real-time reverse transcription-PCR with lyophilized reagentsAmaresh Das
Southeast Poultry Research Laboratory, Agricultural Research Service, USDA, 934 College Station Rd, Athens, GA 30605, USA
J Clin Microbiol 44:3065-73. 2006..The same IPC in the form of armored RNA was also used to monitor the extraction of viral RNA and subsequent detection by RRT-PCR...
Recombination resulting in virulence shift in avian influenza outbreak, ChileDavid L Suarez
Southeast Poultry Research Laboratory, Athens, Georgia, USA
Emerg Infect Dis 10:693-9. 2004..Sequence comparison of all eight gene segments showed the Chilean viruses were also distinct from all other avian influenza viruses and represent a distinct South American clade...
Molecular characterization and typing of chicken and turkey astroviruses circulating in the United States: implications for diagnosticsMary J Pantin-Jackwood
Southeast Poultry Research Laboratory, Agricultural Research Service, U S Department of Agriculture, 934 College Station Rd, Athens, Georgia 30605, USA
Avian Dis 50:397-404. 2006..Based on these data, a revision of the present taxonomic classification for avian astroviruses within the genus Avastrovirus is warranted...
Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virusDavid E Swayne
United States Department of Agriculture, Southeast Poultry Research Laboratory, Agricultural Research Service, 934 College Station Road, Athens, GA 30605, USA
Avian Pathol 35:141-6. 2006..They will be an adjunct to biosecurity measures to reduce virus transmission...
Pekin and Muscovy ducks respond differently to vaccination with a H5N1 highly pathogenic avian influenza (HPAI) commercial inactivated vaccineCaran Cagle
Southeast Poultry Research Laboratory, Exotic and Emerging Avian Viral Diseases Unit, Agricultural Research Service, US Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA
Vaccine 29:6549-57. 2011..This information should be taken into account when developing effective vaccination programs for controlling H5N1 HPAI in different species of ducks...
Detection of H5N1 high-pathogenicity avian influenza virus in meat and tracheal samples from experimentally infected chickensAmaresh Das
Southeast Poultry Research Laboratory, Agricultural Research Service, U S Department of Agriculture, 934 College Station Road, Athens, Georgia 30605, USA
Avian Dis 52:40-8. 2008..In addition, the modified RNA extraction and RRT-PCR test on meat samples provide a rapid and sensitive method of identifying HPAI virus in illegal contraband or domestic meat samples...
An evaluation of avian influenza diagnostic methods with domestic duck specimensErica Spackman
Southeast Poultry Research Laboratory, USDA, Agricultural Research Service, 934 College Station Road, Athens, GA 30605, USA
Avian Dis 53:276-80. 2009..At all time points AGID detected antibody in substantially fewer samples than either ELISA or HI assay...
Multiplex real-time reverse transcription-polymerase chain reaction for the detection of three viruses associated with poult enteritis complex: turkey astrovirus, turkey coronavirus, and turkey reovirusErica Spackman
Southeast Poultry Research Laboratory, USDA ARS, 934 College Station Road, Athens, GA 30605, USA
Avian Dis 49:86-91. 2005..ARV detection by RRT-PCR was compared with virus isolation and had similar sensitivity...
Type A influenza virus detection and quantitation by real-time RT-PCRErica Spackman
Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA
Methods Mol Biol 436:19-26. 2008..This chapter will provide an overview of the USDA-validated rRT-PCR procedure for the detection of type A influenza...
Domestic poultry and SARS coronavirus, southern ChinaDavid E Swayne
Southeast Poultry Research Laboratory, Agricultural Research Service, U S Department of Agriculture, Athens, Georgia 30605, USA
Emerg Infect Dis 10:914-6. 2004..This finding suggests that domestic poultry were unlikely to have been the reservoir, or associated with dissemination, of SARS coronavirus in the animal markets of southern China...
Use of a novel virus inactivation method for a multicenter avian influenza real-time reverse transcriptase-polymerase chain reaction proficiency studyErica Spackman
Southeast Poultry Research Laboratory, USDA-ARS, Athens, GA 30605, USA
J Vet Diagn Invest 17:76-80. 2005..Variation in cycle threshold values among 35 data sets and 490 samples was minimal (CV = 5.19%), and sample identifications were highly accurate (96.7% correct identifications) regardless of real-time PCR instrumentation...
Development of a real-time reverse-transcription PCR for detection of newcastle disease virus RNA in clinical samplesMark G Wise
Southeast Poultry Research Laboratory, Agricultural Research Service, US Department of Agriculture, Athens, Georgia 30605, USA
J Clin Microbiol 42:329-38. 2004..Overall, a positive correlation was obtained between the RRT-PCR results and virus isolation for NDV from clinical samples...
Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypesErica Spackman
Southeast Poultry Research Laboratory, USDA Agricultural Research Service, Athens, Georgia 30605, USA
J Clin Microbiol 40:3256-60. 2002..The remaining samples were positive with only one detection method. Overall the sensitivity and specificity of the H7- and H5-specific RRT-PCR were similar to those of VI and HI...
Avian influenza virus RNA extraction from tissue and swab materialErica Spackman
Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA
Methods Mol Biol 436:13-8. 2008..In this chapter, two well-established procedures for the isolation of AI virus RNA from common clinical specimen types and enriched virus stocks for further molecular applications will be presented...
Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCRErica Spackman
Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA
Methods Mol Biol 436:27-33. 2008..This assay can identify the H5 hemagglutinin of any genetic lineage (North American or Asian) and either pathotype (highly pathogenic or low pathogenic), but does not differentiate between subtypes or pathotypes...
Validation of a real-time reverse transcriptase-PCR assay for the detection of H7 avian influenza virusJanice Pedersen
U S Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Veterinary Services Laboratories, Ames, IA 50010, USA
Avian Dis 54:639-43. 2010..S. Department of Agriculture procedure for the detection of H7 AIV. The 2008 H7 assay replaced the previously used (2002) assay, which does not detect H7 viruses currently circulating in wild birds in North and South America...
Characterization of H5N1 highly pathogenic avian influenza viruses isolated from poultry in Pakistan 2006-2008Jamie L Wasilenko
Southeast Poultry Research Laboratory, USDA Agricultural Research Service, 934 College Station Rd, Athens, GA 30605, USA
Virus Genes 44:247-52. 2012....
Evidence for a new avian paramyxovirus serotype 10 detected in rockhopper penguins from the Falkland IslandsPatti J Miller
Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605, USA
J Virol 84:11496-504. 2010....
Immunogenicity of fowlpox virus expressing the avian influenza virus H5 gene (TROVAC AIV-H5) in catsKemal Karaca
Merial Ltd, 115 Transtech Drive, Athens, Georgia 30601, USA
Clin Diagn Lab Immunol 12:1340-2. 2005..The vaccine described in this study and other poxvirus-vectored vaccines may be of value for the prophylaxis of AI virus-associated morbidity and mortality in mammals...
A brief introduction to the avian influenza virusErica Spackman
Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA
Methods Mol Biol 436:1-6. 2008..e., H1 and H3 in swine, H3 in horses, and H5 and H7 in chickens...
Sequence analysis and receptor specificity of the hemagglutinin of a recent influenza H2N2 virus isolated from chicken in North AmericaLaurel Glaser
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
Glycoconj J 23:93-9. 2006..Sequence analysis revealed that this HA is a member of the Eurasian clade, and receptor binding studies show that it maintains its specificity for the avian influenza virus alpha2-3 linked sialic acid receptor...
Movements of birds and avian influenza from Asia into AlaskaKevin Winker
University of Alaska Museum, Fairbanks, Alaska 99775, USA
Emerg Infect Dis 13:547-52. 2007....
Characteristics of diagnostic tests used in the 2002 low-pathogenicity avian influenza H7N2 outbreak in VirginiaFrancois Elvinger
Department of Large Animal Clinical Sciences, Virginia Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA
J Vet Diagn Invest 19:341-8. 2007....
Influenza A virus infections in land birds, People's Republic of ChinaA Townsend Peterson
Natural History Museum and Biodiversity Research Center, University of Kansas, Lawrence, Kansas 66045, USA
Emerg Infect Dis 14:1644-6. 2008..Influenza A infection was found, particularly among migratory species. Surveillance programs for monitoring spread of these viruses need to be redesigned...
A combination in-ovo vaccine for avian influenza virus and Newcastle disease virusJohn Steel
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
Vaccine 26:522-31. 2008..We propose that this virus has potential as a safe in-ovo live, attenuated, bivalent avian influenza and Newcastle disease virus vaccine...
Rarity of influenza A virus in spring shorebirds, southern AlaskaKevin Winker
Emerg Infect Dis 14:1314-6. 2008
