Robert A Kingsley

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. ncbi Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype
    Robert A Kingsley
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2279-87. 2009
  2. ncbi Fibronectin binding to the Salmonella enterica serotype Typhimurium ShdA autotransporter protein is inhibited by a monoclonal antibody recognizing the A3 repeat
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, College Station, TX 77843, USA
    J Bacteriol 186:4931-9. 2004
  3. ncbi The use of flow cytometry to detect expression of subunits encoded by 11 Salmonella enterica serotype Typhimurium fimbrial operons
    Andrea D Humphries
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, 407 Reynolds Medical Building, College Station 77843-1114, USA
    Mol Microbiol 48:1357-76. 2003
  4. ncbi Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, College Station 77843 1114, USA
    Infect Immun 71:629-40. 2003
  5. ncbi The ShdA adhesin binds to the cationic cradle of the fibronectin 13FnIII repeat module: evidence for molecular mimicry of heparin binding
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, 407 Reynolds Medical Building, College Station, TX77843 1114, USA
    Mol Microbiol 52:345-55. 2004
  6. ncbi Characterization of the genomes of a diverse collection of Salmonella enterica serovar Typhimurium definitive phage type 104
    Fiona J Cooke
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    J Bacteriol 190:8155-62. 2008
  7. ncbi Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux
    Nicola K Petty
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002018. 2011
  8. ncbi A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo
    Angela M Jansen
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002131. 2011
  9. ncbi Molecular pathogenesis of Salmonella enterica serotype typhimurium-induced diarrhea
    Shuping Zhang
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843, USA
    Infect Immun 71:1-12. 2003
  10. ncbi Candidate live, attenuated Salmonella enterica serotype Typhimurium vaccines with reduced fecal shedding are immunogenic and effective oral vaccines
    Moataz Abd El Ghany
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Infect Immun 75:1835-42. 2007

Collaborators

Detail Information

Publications18

  1. ncbi Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype
    Robert A Kingsley
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2279-87. 2009
    ..Typhi are either pseudogenes or are absent. Genome analysis of other epidemic ST313 isolates from Malawi and Kenya provided evidence for microevolution and clonal replacement in the field...
  2. ncbi Fibronectin binding to the Salmonella enterica serotype Typhimurium ShdA autotransporter protein is inhibited by a monoclonal antibody recognizing the A3 repeat
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, College Station, TX 77843, USA
    J Bacteriol 186:4931-9. 2004
    ..Furthermore, a monoclonal antibody that specifically recognized an epitope in a recombinant protein containing the A3 repeat inhibited binding of ShdA to fibronectin...
  3. ncbi The use of flow cytometry to detect expression of subunits encoded by 11 Salmonella enterica serotype Typhimurium fimbrial operons
    Andrea D Humphries
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, 407 Reynolds Medical Building, College Station 77843-1114, USA
    Mol Microbiol 48:1357-76. 2003
    ..These data show that in vivo growth conditions drastically alter the repertoire of fimbrial antigens expressed in S. Typhimurium...
  4. ncbi Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, College Station 77843 1114, USA
    Infect Immun 71:629-40. 2003
    ..These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice...
  5. ncbi The ShdA adhesin binds to the cationic cradle of the fibronectin 13FnIII repeat module: evidence for molecular mimicry of heparin binding
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, 407 Reynolds Medical Building, College Station, TX77843 1114, USA
    Mol Microbiol 52:345-55. 2004
    ..Combined substitution of R6S and R7S strongly reduced ShdA binding to (13)FnIII. These data suggest that ShdA binds the Hep-2 domain of fibronectin by a mechanism that may mimic binding of the host polysaccharide heparin...
  6. ncbi Characterization of the genomes of a diverse collection of Salmonella enterica serovar Typhimurium definitive phage type 104
    Fiona J Cooke
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    J Bacteriol 190:8155-62. 2008
    ..Thus, here the nature of variation in different Salmonella serovar Typhimurium DT104 isolates is further defined at the gene and whole-genome levels, illustrating how this phage type evolves over time...
  7. ncbi Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux
    Nicola K Petty
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002018. 2011
    ..We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease...
  8. ncbi A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo
    Angela M Jansen
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002131. 2011
    ..The presence of the Vi capsule also correlated with induction of the anti-inflammatory cytokine IL-10 in vivo, a factor that impacted on chemotaxis and the activation of immune cells in vitro...
  9. ncbi Molecular pathogenesis of Salmonella enterica serotype typhimurium-induced diarrhea
    Shuping Zhang
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843, USA
    Infect Immun 71:1-12. 2003
  10. ncbi Candidate live, attenuated Salmonella enterica serotype Typhimurium vaccines with reduced fecal shedding are immunogenic and effective oral vaccines
    Moataz Abd El Ghany
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Infect Immun 75:1835-42. 2007
    ..Further, immunization with serotype Typhimurium aroA candidate vaccines harboring shdA and misL afforded complete protection against challenge with a virulent strain of serotype Typhimurium...
  11. ncbi Salmonella bongori provides insights into the evolution of the Salmonellae
    Maria Fookes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002191. 2011
    ..This work suggests that S. bongori has inherited the ancestral Salmonella virulence gene set, but has adapted by incorporating virulence determinants that resemble those employed by EPEC...
  12. ncbi A novel linear plasmid mediates flagellar variation in Salmonella Typhi
    Stephen Baker
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom
    PLoS Pathog 3:e59. 2007
    ..The DNA sequence of pBSSB1 was determined to be just over 27 kbp, and was predicted to encode 33 coding sequences. To our knowledge, pBSSB1 is the first non-bacteriophage-related linear plasmid to be described in the Enterobacteriaceae...
  13. ncbi Salmonella enterica serotype Typhimurium ShdA is an outer membrane fibronectin-binding protein that is expressed in the intestine
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, Reynolds Medical Building, College Station, TX 77843-1114, USA
    Mol Microbiol 43:895-905. 2002
    ....
  14. ncbi Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways
    Nicholas R Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 18:1624-37. 2008
    ..Enteritidis-harboring mutations in functional homologs of the pseudogenes present in S. Gallinarum could provide an experimentally tractable route toward unraveling the genetic basis of host adaptation in S. enterica...
  15. ncbi Population heterogeneity of Salmonella enterica serotype Typhimurium resulting from phase variation of the lpf operon in vitro and in vivo
    Robert A Kingsley
    Department of Medical Microbiology and Immunology, College of Medicine, Texas A and M University System Health Science Center, College Station, Texas 77843-1114, USA
    J Bacteriol 184:2352-9. 2002
    ..These data suggest that elaboration of fimbriae does not have a major influence on the population heterogeneity produced by phase variation of the lpf operon in naïve mice...
  16. ncbi A simple method for directional transcriptome sequencing using Illumina technology
    Nicholas J Croucher
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, Cambridgeshire CB10 1SA, UK
    Nucleic Acids Res 37:e148. 2009
    ..Hence, this simple method should prove a useful tool in aiding genome annotation and gene expression studies in both prokaryotes and eukaryotes...
  17. ncbi Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion
    Anne Bishop
    The Centre for Molecular Microbiology and Infection, Faculty of Life Sciences, Division of Molecular and Cell Biology, Imperial College London, London SW7 2AZ, UK
    Microbiology 154:1914-26. 2008
    ..Mutation of the staA, tcfA or pilS genes did not affect invasion or adhesion in either the presence or the absence of Vi capsule...
  18. ncbi Salmonella enterica serotype Typhimurium and its host-adapted variants
    Wolfgang Rabsch
    National Reference Center for Salmonellae and Other Enteric Pathogens, Robert Koch-Institut, D-38855 Wernigerode, Germany
    Infect Immun 70:2249-55. 2002