Research Topics
Species | Heike FieglerSummaryAffiliation: Wellcome Trust Genome Campus Country: UK Publications
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Detail Information
Publications
Array comparative genomic hybridization analysis of colorectal cancer cell lines and primary carcinomasEleanor J Douglas
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Cancer Res 64:4817-25. 2004..3 amplification a common finding in MSI+ samples. A number of genes of interest are located within the frequently aberrated regions, which are likely to be of importance in the development and progression of CRC...- Breaking the waves: improved detection of copy number variation from microarray-based comparative genomic hybridizationJohn C Marioni
Computational Biology Group, Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Centre for Mathematical Sciences, Wilberforce Road, Cambridge CB3 0WA, UK
Genome Biol 8:R228. 2007..However, methods for analyzing the complex data produced and identifying regions of CNV are still being refined... - Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11Katleen De Preter
Center for Medical Genetics, Ghent University Hospital, MRB 2nd floor, De Pintelaan 185, B 9000 Ghent, Belgium
BMC Genomics 6:97. 2005..We decided to further exploit this model system as a means to identify candidate tumour suppressor or differentiation genes located on chromosome 11... - High resolution array-CGH analysis of single cellsHeike Fiegler
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nucleic Acids Res 35:e15. 2007..Our results demonstrate the potential of this technology for studies of tumor biology and for clinical diagnostics... - Construction and use of spotted large-insert clone DNA microarrays for the detection of genomic copy number changesHeike Fiegler
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nat Protoc 2:577-87. 2007..According to our protocols, the procedure will take approximately 3 days from labeling the DNA to scanning the hybridized slides... - Deletion at chromosome band 20p12.1 in colorectal cancer revealed by high resolution array comparative genomic hybridizationEleanor J Davison
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Genes Chromosomes Cancer 44:384-91. 2005..Sequence changes in BA318C17.1 and reduced expression of both genes was detected, suggesting that the abrogation of these genes may play a role in colorectal tumorigenesis... - Genomic array technologyHeike Fiegler
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
Methods Cell Biol 75:769-85. 2004 - Accurate and reliable high-throughput detection of copy number variation in the human genomeHeike Fiegler
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
Genome Res 16:1566-74. 2006..Based on these studies, we developed a variance-based automatic copy number detection analysis process (CNVfinder) and have demonstrated its robustness by comparison with the SW-ARRAY method... - Global variation in copy number in the human genomeRichard Redon
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nature 444:444-54. 2006..The data obtained delineate linkage disequilibrium patterns for many CNVs, and reveal marked variation in copy number among populations. We also demonstrate the utility of this resource for genetic disease studies... - A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridizationYeun Jun Chung
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Genome Res 14:188-96. 2004..The resulting 3K mouse BAC microarrays reproducibly identified DNA copy number alterations in cell lines and primary tumors, such as single-copy deletions, regional amplifications, and aneuploidy... - Replication timing of human chromosome 6Kathryn Woodfine
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Cell Cycle 4:172-6. 2005..Positive correlations are observed between replication timing and a number of genomic features including GC content, repeat content and transcriptional activity... - Germline rates of de novo meiotic deletions and duplications causing several genomic disordersDaniel J Turner
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, CB10 1SA, UK
Nat Genet 40:90-5. 2008.... - Genomic profiling identifies discrete deletions associated with translocations in glioblastoma multiformePaul J Mulholland
Human Cytogenetics Laboratory, Cancer Research, UK
Cell Cycle 5:783-91. 2006.... - A complex rearrangement on chromosome 22 affecting both homologues; haplo-insufficiency of the Cat eye syndrome region may have no clinical relevanceMarjolein Kriek
Center for Human and Clinical Genetics, Leiden University Medical Center, Einthovenweg, 2300 RC, Leiden, The Netherlands
Hum Genet 120:77-84. 2006..This study highlights the value of using different genomic approaches to unravel chromosomal alterations in order to study their phenotypic impact... - Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex changes and multiple forms of chromosomal instability in colorectal cancersMichelle Gaasenbeek
Molecular and Population Genetics Laboratory, London Research Institute, Cancer Research UK, London WC2A 3PX, UK
Cancer Res 66:3471-9. 2006..These data suggest that CIN is not synonymous with copy number change and some cancers have a specific tendency to whole-chromosome deletion and regain or to mitotic recombination... - Guidelines for molecular karyotyping in constitutional genetic diagnosisJoris Robert Vermeesch
Center for Human Genetics, University Hospital Gasthuisberg, Leuven, Belgium
Eur J Hum Genet 15:1105-14. 2007..Internal and external quality control programmes are urgently needed to evaluate and standardise the test results between laboratories... - Analysis of ovarian cancer cell lines using array-based comparative genomic hybridizationMaryou B K Lambros
Molecular and Population Genetics Laboratory, Cancer Research UK, London, UK
J Pathol 205:29-40. 2005..Other potential oncogenes, which mapped to regions found by this study, included cyclin E and PIK3C2G. Candidate tumour suppressor genes in regions of loss included CDKN2C, SMAD4-interacting protein and RASSF2... - High-resolution analysis of genomic copy number alterations in bladder cancer by microarray-based comparative genomic hybridizationCarolyn D Hurst
Cancer Research UK Clinical Centre, St James's University Hospital, Beckett St, Leeds LS9 7TF, UK
Oncogene 23:2250-63. 2004..3 being the most frequent. Real-time PCR analysis revealed a novel candidate gene with consistent overexpression in all cell lines with the 6p22.3 amplicon... - DNA microarrays for comparative genomic hybridization based on DOP-PCR amplification of BAC and PAC clonesHeike Fiegler
Wellcome Trust Sanger Institute/Cancer Research UK Genomic Microarray Group, Hinxton, Cambridge, CB10 1SA, United Kingdom
Genes Chromosomes Cancer 36:361-74. 2003.... - Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot projectEwan Birney
Nature 447:799-816. 2007..Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function... - Replication-timing-correlated spatial chromatin arrangements in cancer and in primate interphase nucleiFlorian Grasser
Department of Biology II, Human Genetics, Ludwig Maximilians University Munich, Planegg Martinsreid, Germany
J Cell Sci 121:1876-86. 2008.... - Determination of donor-type chimerism using a semi-quantitative PCR-based method in a canine model for bone marrow transplantationHeike Fiegler
, , 81377, , Germany
Vet Immunol Immunopathol 84:61-70. 2002..The established semi-quantitative PCR assay offers a quick, simple, accurate and sensitive way of evaluating and quantitating the percentage of chimerism in a sex-mismatched canine BMT model... - Micro-array analyses decipher exceptional complex familial chromosomal rearrangementChristine Fauth
, , Trogerstr. 32, , Germany
Hum Genet 119:145-53. 2006..We detail the most complicated familial complex chromosomal rearrangement reported to date and thus an extreme example of inheritance of chromosomal rearrangements without error in meiotic segregation... - Genome-wide screening of genomic alterations and their clinicopathologic implications in non-small cell lung cancersTae-Min Kim
Department of Microbiology, College of Medicine, Catholic University of Korea, Socho-gu, Seoul, Korea
Clin Cancer Res 11:8235-42. 2005..The novel genomic alterations identified in this study, along with their clinicopathologic implications, would be useful to elucidate the molecular mechanisms of lung cancer and to identify reliable biomarkers for clinical application... - Genomic and protein expression profiling identifies CDK6 as novel independent prognostic marker in medulloblastomaFrank Mendrzyk
Division of Molecular Genetics B060, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
J Clin Oncol 23:8853-62. 2005..Identification of molecular markers for prognosis and development of novel pathogenesis-based therapies depends crucially on a better understanding of medulloblastoma pathomechanisms... - Investigating chromosome organization with genomic microarraysKathryn Woodfine
Department of Medical and Molecular Genetics, GKT School of Medicine, King's College London, London, SE1 9RT, UK
Chromosome Res 13:249-57. 2005..Furthermore, by application of S phase fractions to genomic microarrays, replication timing can be estimated. Thus, microarrays can provide new information about chromosome structure and gene regulation... - Array-CGH analysis of microsatellite-stable, near-diploid bowel cancers and comparison with other types of colorectal carcinomaAngela M Jones
Molecular and Population Genetics Laboratory, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
Oncogene 24:118-29. 2005..In conclusion, our data support the suggestion that some MSI-CIN- carcinomas form a qualitatively different group from the other cancer types, and also suggest that the MSI-CIN- group is itself heterogeneous... - Chromatin architecture of the human genome: gene-rich domains are enriched in open chromatin fibersNick Gilbert
MRC Human Genetics Unit, Edinburgh, EH4 2XU, Scotland
Cell 118:555-66. 2004..We suggest that domains of open chromatin may create an environment that facilitates transcriptional activation and could provide an evolutionary constraint to maintain clusters of genes together along chromosomes... - Replication timing of the human genomeKathryn Woodfine
The Welcome Trust Sanger Institute, Welcome Genome Campus, Cambridge, UK
Hum Mol Genet 13:191-202. 2004..We show a positive correlation, both genome-wide and at a high resolution, between replication timing and a range of genome parameters including GC content, gene density and transcriptional activity... - An integrated resource for genome-wide identification and analysis of human tissue-specific differentially methylated regions (tDMRs)Vardhman K Rakyan
Institute of Cell and Molecular Science, Barts and The London, London E1 2AT, United Kingdom
Genome Res 18:1518-29. 2008....