Research Topics
| Donald WlodkowicSummaryAffiliation: University of Glasgow Country: UK Publications
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Detail Information
Publications
Real-time cytotoxicity assaysDonald Wlodkowic
The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
Methods Mol Biol 731:285-91. 2011..Considering vast time savings and kinetic data acquisition such assays have the potential to be applied in a number of areas including accelerated anticancer drug discovery and high-throughput screening routines...- Microfluidic cell arrays in tumor analysis: new prospects for integrated cytomicsDonald Wlodkowic
The Bioelectronics Research Centre, University of Glasgow, Rankine Building, Oakfiled Avenue, Glasgow, G12 8LT, UK
Expert Rev Mol Diagn 10:521-30. 2010..Lab-on-a-chip microfluidic cell arrays therefore provide new opportunities for the development of content-rich personalized clinical diagnostics and higher-throughput clinical screening in oncology... - Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometryDonald Wlodkowic
Department of Electronics and Electrical Engineering, The Bioelectronics Research Center, University of Glasgow, Glasgow, UK
Exp Cell Res 315:1706-14. 2009..In particular, it should allow for dynamic analysis of samples where low cell number has so far been an obstacle, e.g. primary cancer stems cells or circulating minimal residual tumors... - Microfabricated analytical systems for integrated cancer cytomicsDonald Wlodkowic
The Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow G12 8LT, UK
Anal Bioanal Chem 398:193-209. 2010..It is largely anticipated that advances in microfluidic technologies should aid in tailoring of investigational therapies and support the current computational efforts in systems biology... - Cytometry in cell necrobiology revisited. Recent advances and new vistasDonald Wlodkowic
The Bioelectronics Research Centre, University of Glasgow, Glasgow, United Kingdom
Cytometry A 77:591-606. 2010..This review highlights the key developments warranting a renaissance and evolution of cytometric techniques in the field of cell necrobiology... - Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cellsDonald Wlodkowic
The Bioelectronics Research Center, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT Glasgow, UK
Anal Chem 81:6952-9. 2009.... - Biological implications of polymeric microdevices for live cell assaysDonald Wlodkowic
Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, University of Glasgow, Glasgow, UK G12 8LT
Anal Chem 81:9828-33. 2009..Results show that PDMS is nontoxic to cells, as their viability and growth characteristic in PDMS-based platforms is comparable to that of their polystyrene counterparts... - Apoptosis and beyond: cytometry in studies of programmed cell deathDonald Wlodkowic
Biomems Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
Methods Cell Biol 103:55-98. 2011..Strategies of selecting the optimal multiparameter approaches, as well as potential difficulties in the experimental procedures, are thoroughly summarized... - Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresisKhashayar Khoshmanesh
Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand
Anal Chem 83:3217-21. 2011..This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells... - Microfluidic single-cell array cytometry for the analysis of tumor apoptosisDonald Wlodkowic
The Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT, United Kingdom
Anal Chem 81:5517-23. 2009.... - Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probesDonald Wlodkowic
The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland 1142, New Zealand
Methods Mol Biol 740:81-9. 2011.... - Wormometry-on-a-chip: Innovative technologies for in situ analysis of small multicellular organismsDonald Wlodkowic
Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, 1142, New Zealand
Cytometry A 79:799-813. 2011..We also introduce a new term "wormometry" to collectively distinguish these up-and-coming chip-based technologies that go far beyond the conventional meaning of the term "cytometry.".. - Trapping and imaging of micron-sized embryos using dielectrophoresisKhashayar Khoshmanesh
The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
Electrophoresis 32:3129-32. 2011..This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force... - Apoptosis goes on a chip: advances in the microfluidic analysis of programmed cell deathDonald Wlodkowic
The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
Anal Chem 83:6439-46. 2011.... - Multiparameter detection of apoptosis using red-excitable SYTO probesDonald Wlodkowic
Department of Biological Sciences, Glasgow Caledonian University, Glasgow, United Kingdom
Cytometry A 73:563-9. 2008..Considering simplicity of staining protocols, low cost, and avoidance of spectral compensation problems, we expect that red-excitable SYTO dyes will find a wide range of cytometric applications... - Cell death goes LIVE: technological advances in real-time tracking of cell deathJoanna Skommer
School of Biological Sciences, University of Auckland, Auckland, New Zealand
Cell Cycle 9:2330-41. 2010..Here, we review current status of the most innovative analytical technologies for dynamic tracking of cell death, and address the interdisciplinary promises and future challenges of these methods... - Flow cytometry-based apoptosis detectionDonald Wlodkowic
The Bioelectronics Research Center, University of Glasgow, Rankine Building, Glasgow G12 8LT, UK
Methods Mol Biol 559:19-32. 2009..In the present chapter, we outline a handful of commonly used methods that are based on the assessment of: mitochondrial transmembrane potential, activation of caspases, plasma membrane alterations and DNA fragmentation... - SYTO probes: markers of apoptotic cell demiseDonald Wlodkowic
University of Glasgow, UK
Curr Protoc Cytom . 2007.... - SYTO probes in the cytometry of tumor cell deathDonald Wlodkowic
The Bioelectronics Research Center, University of Glasgow, Glasgow, United Kingdom
Cytometry A 73:496-507. 2008..Finally, we provide a future outlook on SYTO probes in cytometric and microfluidics (Lab-on-a-Chip) high content analysis applications... - Rise of the micromachines: microfluidics and the future of cytometryDonald Wlodkowic
The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand
Methods Cell Biol 102:105-25. 2011.... - ER-Golgi network--a future target for anti-cancer therapyDonald Wlodkowic
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, UK
Leuk Res 33:1440-7. 2009..As the interest in the role of ER-Golgi network during cancer cell death has been gaining momentum, we attempt here to critically appraise current status of development of investigational anti-cancer agents that target ER and/or Golgi... - Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cellsShannon L Faley
Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow, UK G12 8LT
Lab Chip 9:2659-64. 2009..Further studies show that dasatinib does, however, markedly reduce CML stem/progenitor cell migration in situ... - Dynamic analysis of drug-induced cytotoxicity using chip-based dielectrophoretic cell immobilization technologyKhashayar Khoshmanesh
Department of Chemistry and MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand
Anal Chem 83:2133-44. 2011..This ability is of particular importance in studying the outcome of patient derived cancer cells, when exposed to therapeutic drugs, as these cells are often rare and difficult to collect, purify and immobilize... - Timing is everything: stochastic origins of cell-to-cell variability in cancer cell deathJoanna Skommer
School of Biological Sciences, University of Auckland, Auckland 1142, New Zealand
Front Biosci 16:307-14. 2011..Here we summarize our current understanding of the origins of stochastic variability in cells' commitment to apoptosis, and its implications in the field on cancer therapy... - Tumors on chips: oncology meets microfluidicsDonald Wlodkowic
Auckland Microfabrication Facility, Department of Chemistry, University of Auckland, Auckland, New Zealand
Curr Opin Chem Biol 14:556-67. 2010..It is envisaged that microfluidic solutions may well represent the platform of choice for next generation in vitro cancer models... - Microfluidics: Emerging prospects for anti-cancer drug screeningDonald Wlodkowic
Donald Wlodkowic, Auckland Microfabrication Facility, Department of Chemistry, University of Auckland, 1142 Auckland, New Zealand
World J Clin Oncol 1:18-23. 2010..Herein we highlight the selected advancements in this emerging field of bioengineering, and provide a snapshot of developments with relevance to anti-cancer drug screening routines...