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Genomes and Genes | M S PagetSummaryAffiliation: University of Sussex Country: UK Publications
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Publications
Defining the disulphide stress response in Streptomyces coelicolor A3(2): identification of the sigmaR regulonM S Paget
Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, UK
Mol Microbiol 42:1007-20. 2001..We present DNA sequence evidence that many of the targets identified in S. coelicolor are also under the control of the sigmaR homologue in the actinomycete pathogen Mycobacterium tuberculosis...- Mutational analysis of RsrA, a zinc-binding anti-sigma factor with a thiol-disulphide redox switchM S Paget
Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, UK
Mol Microbiol 39:1036-47. 2001..Unexpectedly, rsrA null mutants were blocked in sporulation, probably as a consequence of an increase in the level of free sigmaR... - The sigma70 family of sigma factorsMark S B Paget
School of Biological Sciences, University of Sussex, Brighton BN1 9QG, UK
Genome Biol 4:203. 2003..These structural data are starting to illuminate the mechanistic role of sigma factors in transcription initiation... - Thiol-based regulatory switchesMark S B Paget
Department of Biochemistry, School of Life Sciences, University of Sussex, Brighton BN1 9QG, United Kingdom
Annu Rev Genet 37:91-121. 2003..In this review, we highlight these issues, focusing on seven well-characterized microbial proteins controlled by thiol-based switches, each of which exhibits unique regulatory features... - A putative two-component signal transduction system regulates sigmaE, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2)M S Paget
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
Mol Microbiol 33:97-107. 1999..We propose a model in which the CseB/CseC two-component system modulates activity of the sigE promoter in response to signals from the cell envelope... - sigmaR, an RNA polymerase sigma factor that modulates expression of the thioredoxin system in response to oxidative stress in Streptomyces coelicolor A3(2)M S Paget
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
EMBO J 17:5776-82. 1998..We present evidence that the sigmaR system also functions in the actinomycete pathogen Mycobacterium tuberculosis... - Evidence that the extracytoplasmic function sigma factor sigmaE is required for normal cell wall structure in Streptomyces coelicolor A3(2)M S Paget
John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom
J Bacteriol 181:204-11. 1999..Growth on media deficient in magnesium caused the induction of both hrdD promoters in a sigE-dependent manner... - The Role of zinc in the disulphide stress-regulated anti-sigma factor RsrA from Streptomyces coelicolorWei Li
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
J Mol Biol 333:461-72. 2003..Redox stress induces disulphide bond formation amongst zinc-ligating residues, expelling the metal ion and stabilising a structure incapable of binding the sigma factor... - Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticusG H Jones
Department of Genetics, John Innes Centre, Colney, Norwich, UK
Mol Microbiol 23:169-78. 1997..The loss of actinomycin production correlated with a 10-fold decrease in the activity of actinomycin synthetase I, the enzyme which catalyses the activation of the precursor of the actinomycin chromophore... - Zinc-responsive regulation of alternative ribosomal protein genes in Streptomyces coelicolor involves zur and sigmaRGillian A Owen
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
J Bacteriol 189:4078-86. 2007..coelicolor. The induction of sigma(R) activity during zinc limitation establishes an important link between thiol-disulfide metabolism and zinc homeostasis... - Characterization of an inducible vancomycin resistance system in Streptomyces coelicolor reveals a novel gene (vanK) required for drug resistanceHee Jeon Hong
Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, UK
Mol Microbiol 52:1107-21. 2004..Analysis of mutants defective in the vanRS and cseBC cell envelope signal transduction systems revealed significant cross-talk between the two pathways... - Identification and structure of the anti-sigma factor-binding domain of the disulphide-stress regulated sigma factor sigma(R) from Streptomyces coelicolorWei Li
School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, UK
J Mol Biol 323:225-36. 2002.... - A signal transduction system in Streptomyces coelicolor that activates the expression of a putative cell wall glycan operon in response to vancomycin and other cell wall-specific antibioticsHee Jeon Hong
Department of Molecular Microbiology, John Innes Centre, Colney, Norwich NR4 7UH, UK
Mol Microbiol 44:1199-1211. 2002..Based on these data, we propose a model for the regulation and function of the sigmaE signal transduction system... - The RNA polymerase-binding protein RbpA confers basal levels of rifampicin resistance on Streptomyces coelicolorKaty V Newell
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
Mol Microbiol 60:687-96. 2006..The data presented suggest that RbpA confers basal levels of rifampicin resistance and is a novel regulator of rRNA synthesis in S. coelicolor... - The sigmaR regulon of Streptomyces coelicolor A32 reveals a key role in protein quality control during disulphide stressDimitris Kallifidas
Department of Chemistry and Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
Microbiology 156:1661-72. 2010..This work shows that the sigma(R)-RsrA system plays a more significant role in protein quality control than previously realized, and emphasizes the importance of controlling the cellular thiol-disulphide redox balance... - The zinc-responsive regulator Zur controls expression of the coelibactin gene cluster in Streptomyces coelicolorDimitris Kallifidas
Department of Chemistry and Biochemistry, University of Sussex, Falmer, Brighton BN1 9QG, UK
J Bacteriol 192:608-11. 2010..Disruption of a key coelibactin biosynthetic gene suppressed the Zur sporulation phenotype, suggesting that deregulation of coelibactin synthesis inhibits sporulation... - A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2)Dimitris Brekasis
Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK
EMBO J 22:4856-65. 2003..Rex homologues exist in most Gram-positive bacteria, including human pathogens such as Staphylococcus aureus, Listeria monocytogenes and Streptococcus pneumoniae... - X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensingE Allen Sickmier
Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
Structure 13:43-54. 2005..We show that T-Rex binds to the Rex operator, and NADH but not NAD(+) inhibits T-Rex/DNA binding activity. A mechanism for redox sensing by Rex family members is proposed by analogy with domain closure of NAD(H)-dependent enzymes... - Assignment of the zinc ligands in RsrA, a redox-sensing ZAS protein from Streptomyces coelicolorKonrad Zdanowski
Department of Biology (Area 10, University of York, P.O. Box 373, York YO10 5YW, UK
Biochemistry 45:8294-300. 2006..Cys11 and Cys44 form the trigger disulfide in RsrA, explaining why the metal ion is expelled during oxidation. We discuss these data in the context of redox sensing by RsrA and the sensory mechanisms of other ZAS proteins...