Frank H Niesen

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. ncbi An automated in vitro protein folding screen applied to a human dynactin subunit
    Christoph Scheich
    Proteinstrukturfabrik, Heubnerweg 6, Berlin 14059, Germany
    Protein Sci 13:370-80. 2004
  2. ncbi X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
    Babu A Manjasetty
    Protein Structure Factory, c o BESSY GmbH, Albert Einstein Str 15, 12489 Berlin, Germany
    BMC Struct Biol 5:21. 2005
  3. ncbi The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability
    Frank H Niesen
    Structural Genomics Consortium, Botnar Research Centre, Oxford University, Oxford, UK
    Nat Protoc 2:2212-21. 2007
  4. ncbi An approach to quality management in structural biology: biophysical selection of proteins for successful crystallization
    Frank H Niesen
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, D 10117 Berlin, Germany
    J Struct Biol 162:451-9. 2008
  5. ncbi Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation
    Peter Rellos
    University of Oxford, Nuffield Department of Clinical Medicine, Structural Genomics Consortium, Oxford, United Kingdom
    PLoS Biol 8:e1000426. 2010
  6. ncbi Kinase inhibitor selectivity profiling using differential scanning fluorimetry
    Oleg Fedorov
    Structural Genomics Consortium, Oxford University, Oxford, UK
    Methods Mol Biol 795:109-18. 2012
  7. ncbi Kinase domain insertions define distinct roles of CLK kinases in SR protein phosphorylation
    Alex N Bullock
    Structural Genomics Consortium, University of Oxford, Old Road Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
    Structure 17:352-62. 2009
  8. ncbi High-affinity inhibitors of human NAD-dependent 15-hydroxyprostaglandin dehydrogenase: mechanisms of inhibition and structure-activity relationships
    Frank H Niesen
    Structural Genomics Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e13719. 2010
  9. ncbi Structure of dystrophia myotonica protein kinase
    Jonathan M Elkins
    Structural Genomics Consortium, Nuffield Department of Medicine, Oxford University, Old Road Campus Research Building, Oxford, OX3 7DQ, United Kingdom
    Protein Sci 18:782-91. 2009
  10. ncbi The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins
    Opher Gileadi
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford, UK
    J Struct Funct Genomics 8:107-19. 2007

Collaborators

Detail Information

Publications18

  1. ncbi An automated in vitro protein folding screen applied to a human dynactin subunit
    Christoph Scheich
    Proteinstrukturfabrik, Heubnerweg 6, Berlin 14059, Germany
    Protein Sci 13:370-80. 2004
    ..coli. The described screen allows for high-throughput folding analysis of inclusion body proteins for structural and functional analyses...
  2. ncbi X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
    Babu A Manjasetty
    Protein Structure Factory, c o BESSY GmbH, Albert Einstein Str 15, 12489 Berlin, Germany
    BMC Struct Biol 5:21. 2005
    ..Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of arterial smooth muscle cells (SMCs)...
  3. ncbi The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability
    Frank H Niesen
    Structural Genomics Consortium, Botnar Research Centre, Oxford University, Oxford, UK
    Nat Protoc 2:2212-21. 2007
    ..Results can be obtained in a single day...
  4. ncbi An approach to quality management in structural biology: biophysical selection of proteins for successful crystallization
    Frank H Niesen
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, D 10117 Berlin, Germany
    J Struct Biol 162:451-9. 2008
    ..In summary, our study demonstrates that biophysical characterization provides an ideal tool to facilitate quality management for structural biology and many other areas of biological research...
  5. ncbi Structure of the CaMKIIdelta/calmodulin complex reveals the molecular mechanism of CaMKII kinase activation
    Peter Rellos
    University of Oxford, Nuffield Department of Clinical Medicine, Structural Genomics Consortium, Oxford, United Kingdom
    PLoS Biol 8:e1000426. 2010
    ..Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the Web plugin are available in Text S1...
  6. ncbi Kinase inhibitor selectivity profiling using differential scanning fluorimetry
    Oleg Fedorov
    Structural Genomics Consortium, Oxford University, Oxford, UK
    Methods Mol Biol 795:109-18. 2012
    ..Comparative studies revealed that generated data is highly reproducible and correlates well with the results from other ligand binding methodologies, direct binding constants as well as enzymatic assays...
  7. ncbi Kinase domain insertions define distinct roles of CLK kinases in SR protein phosphorylation
    Alex N Bullock
    Structural Genomics Consortium, University of Oxford, Old Road Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
    Structure 17:352-62. 2009
    ..In addition, despite high sequence conservation, we identified inhibitors with surprising isoform specificity for CLK1 over CLK3...
  8. ncbi High-affinity inhibitors of human NAD-dependent 15-hydroxyprostaglandin dehydrogenase: mechanisms of inhibition and structure-activity relationships
    Frank H Niesen
    Structural Genomics Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom
    PLoS ONE 5:e13719. 2010
    ..High-affinity probes for 15-PGDH will, therefore, represent important tools for further studies...
  9. ncbi Structure of dystrophia myotonica protein kinase
    Jonathan M Elkins
    Structural Genomics Consortium, Nuffield Department of Medicine, Oxford University, Old Road Campus Research Building, Oxford, OX3 7DQ, United Kingdom
    Protein Sci 18:782-91. 2009
    ..The BIM-8 inhibitor occupies the ATP site and has similar binding mode as observed in PDK1...
  10. ncbi The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins
    Opher Gileadi
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford, UK
    J Struct Funct Genomics 8:107-19. 2007
    ....
  11. ncbi Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites
    Ashley C W Pike
    Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford, UK
    EMBO J 27:704-14. 2008
    ..Based on the presented structural and functional data, a model for specific activation segment phosphorylation at non-consensus substrate sites is proposed that is likely to be common to other kinases from diverse subfamilies...
  12. ncbi Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization
    Andrew P Turnbull
    Max Delbruck Centrum fur Molekulare Medizin, Berlin, Germany
    EMBO J 24:875-84. 2005
    ..A conformational change may facilitate palmitoyl extrusion from BET3 and allow the fatty acid chain to engage in intermolecular hydrophobic interactions...
  13. ncbi Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, ON, Canada M5G 1L5
    Proc Natl Acad Sci U S A 103:15835-40. 2006
    ..The methods are cost-effective, can be implemented in any laboratory, promise to increase the success rates of purifying and crystallizing human proteins significantly, and identify new ligands for these proteins...
  14. ncbi X-ray structure of fumarylacetoacetate hydrolase family member Homo sapiens FLJ36880
    Babu A Manjasetty
    Protein Structure Factory, c o BESSY GmbH, Albert Einstein Str 15, D 12489 Berlin, Germany
    Biol Chem 385:935-42. 2004
    ..Two point mutations in the active-site of FAH, responsible for the metabolic disease hereditary tyrosinemia type I (HTI) in humans, affect residues that are structurally conserved in FLJ36880 and located in the putative catalytic site...
  15. ncbi A catalog of human cDNA expression clones and its application to structural genomics
    Konrad Büssow
    Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany
    Genome Biol 5:R71. 2004
    ..Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures...
  16. ncbi Rapid purification and crystal structure analysis of a small protein carrying two terminal affinity tags
    Uwe Mueller
    , , Takustr. 6, D-14195 Berlin, Germany
    J Struct Funct Genomics 4:217-25. 2003
    ....
  17. ncbi Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold
    Babu A Manjasetty
    Protein Structure Factory, Berlin 12489, Germany
    Protein Sci 15:914-20. 2006
    ..Biochemical assays failed to demonstrate any of these enzyme activities in PTD012. However, PTD012 exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate...
  18. ncbi Crystal structure of Homo sapiens protein hp14.5
    Babu A Manjasetty
    Protein Structure Factory, c/o BESSY GmbH, Berlin, Germany
    Proteins 54:797-800. 2004