Research Topics
Genomes and Genes | A Paul MouldSummaryAffiliation: University of Manchester Country: UK Publications
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Publications
Role of ADMIDAS cation-binding site in ligand recognition by integrin alpha 5 beta 1A Paul Mould
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
J Biol Chem 278:51622-9. 2003....- Integrin activation involves a conformational change in the alpha 1 helix of the beta subunit A-domainA Paul Mould
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
J Biol Chem 277:19800-5. 2002..The mechanism of beta subunit A-domain activation appears to be distinct from that of the A-domains found in some integrin alpha subunits... - Conformational changes in the integrin beta A domain provide a mechanism for signal transduction via hybrid domain movementA Paul Mould
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
J Biol Chem 278:17028-35. 2003..Taken together, these data suggest that the transduction of conformational changes through betaA involves shape shifting in the alpha7 helix region, which is linked to a swing of the hybrid domain away from the alpha subunit... - Regulation of integrin function through conformational complexity: not simply a knee-jerk reaction?A Paul Mould
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
Curr Opin Cell Biol 16:544-51. 2004..This conformational complexity is likely to permit fine-tuning of integrin function through regulation of ligand-binding affinity and intracellular signalling... - Structure of an integrin-ligand complex deduced from solution x-ray scattering and site-directed mutagenesisA Paul Mould
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
J Biol Chem 278:39993-9. 2003..Our data provide the first view of a complex between an integrin and a macromolecular ligand in solution, at a nominal resolution of approximately 10 A... - Evidence that monoclonal antibodies directed against the integrin beta subunit plexin/semaphorin/integrin domain stimulate function by inducing receptor extensionA Paul Mould
Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
J Biol Chem 280:4238-46. 2005..Second, the PSI domain is important for the transduction of conformational changes from the knee to head. Third, unbending is likely to provide a general mechanism for control of integrin-ligand recognition... - Dual functionality of the anti-beta1 integrin antibody, 12G10, exemplifies agonistic signalling from the ligand binding pocket of integrin adhesion receptorsJonathan D Humphries
Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
J Biol Chem 280:10234-43. 2005..We propose that either ligand-specific transmembrane conformational changes or ligand-specific differences in the kinetics of transmembrane domain separation underlie integrin agonism... - Novel activating and inactivating mutations in the integrin beta1 subunit A domainStephanie J Barton
The Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
Biochem J 380:401-7. 2004..Although these changes are grossly similar to those defined in the A domains found in some integrin alpha subunits, movement of the alpha1 helix appears to play a more prominent role in betaA domain activation... - Distinct roles of beta1 metal ion-dependent adhesion site (MIDAS), adjacent to MIDAS (ADMIDAS), and ligand-associated metal-binding site (LIMBS) cation-binding sites in ligand recognition by integrin alpha2beta1Dimitra Valdramidou
Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
J Biol Chem 283:32704-14. 2008.... - Mapping the ligand-binding pocket of integrin alpha5beta1 using a gain-of-function approachA Paul Mould
Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
Biochem J 424:179-89. 2009..These results will aid the development of reagents that block integrin alpha5beta1 functions in vivo... - Interaction of filamin A with the integrin beta 7 cytoplasmic domain: role of alternative splicing and phosphorylationMark A Travis
School of Biological Sciences, 2 205 Stopford Building, Wellcome Trust Centre for Cell Matrix Research, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
FEBS Lett 569:185-90. 2004..In addition, we provide evidence that phosphorylation of the splice variant region is unlikely to represent the mechanism by which binding is reduced... - Integrin structure: heady advances in ligand binding, but activation still makes the knees wobbleMartin J Humphries
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, 2 205 Stopford Building, Oxford Road, Manchester, UK M13 9PT
Trends Biochem Sci 28:313-20. 2003..Like all major advances, however, these studies have raised more questions than they have answered about issues such as the mechanisms underlying ligand-binding specificity and long-range conformational regulation... - Mapping functional residues onto integrin crystal structuresMartin J Humphries
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, United Kingdom
Curr Opin Struct Biol 13:236-43. 2003..In the past two years, this combining of structure and function has led to the development of novel hypotheses for the mechanisms of integrin regulation... - Linking integrin conformation to functionJanet A Askari
Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
J Cell Sci 122:165-70. 2009..In this Commentary, we pose some of the key remaining questions that surround integrin structure-function relationships and review the evidence that supports the current models... - E-cadherin is a ligand for integrin alpha2beta1John D Whittard
Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
Matrix Biol 21:525-32. 2002..These findings demonstrate that E-cadherin can interact with alpha2beta1 and suggest that heterotypic interactions between E-cadherin and integrins may be more common than originally thought... - Analyzing integrin-dependent adhesionA Paul Mould
University of Manchester, Manchester, United Kingdom
Curr Protoc Cell Biol . 2011..These assays can be used, e.g., to measure apparent affinities of integrins for different ligands and IC(50) values of pharmacological inhibitors... - Identification of multiple integrin beta1 homologs in zebrafish (Danio rerio)A Paul Mould
Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
BMC Cell Biol 7:24. 2006.... - Analyzing integrin-dependent adhesionA Paul Mould
University of Manchester, Manchester, United Kingdom
Curr Protoc Cell Biol . 2002..The unit also contains support protocols for integrin purification and coupling of antibodies to Sepharose for use in this purification, as well as for biotinylation of integrin ligands to be used in the solid-phase assay... - The "linker" region (amino acids 38-47) of the disintegrin elegantin is a novel inhibitory domain of integrin alpha5beta1-dependent cell adhesion on fibronectin: evidence for the negative regulation of fibronectin synergy site biological activitRushika Sumathipala
Laboratory of Thrombosis and Vascular Remodelling, Division of Cardiovascular Medicine, King's College London School of Medicine, St Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, United Kingdom
J Biol Chem 281:37686-96. 2006..These data demonstrate a novel biological function for a disintegrin domain that antagonizes integrin-mediated cell adhesion... - Evidence for the presence of a low-mass beta1 integrin on the cell surfaceXiaobo Meng
Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, 715 McDermot Avenue, Winnipeg, MB, Canada R3E 3P4
J Cell Sci 118:4009-16. 2005..The data also suggest that differences in glycosylation may be involved in defining the association between the integrin alpha and beta chains and the functional properties of these integrins... - The integrins of the urochordate Ciona intestinalis provide novel insights into the molecular evolution of the vertebrate integrin familyRichard Ewan
Plant Molecular Science Group, University of Glasgow, Bower Building, G12 8QQ, UK
BMC Evol Biol 5:31. 2005..The publication of the genome of a basal chordate, Ciona intestinalis, provides a unique opportunity to gain further insight into how and when the expanded integrin supergene family found in vertebrates evolved... - A specific alpha5beta1-integrin conformation promotes directional integrin translocation and fibronectin matrix formationKatherine Clark
Craniofacial Developmental Biology and Regeneration Branch, NIDCR, NIH, Bethesda, MA 20892, USA
J Cell Sci 118:291-300. 2005.... - Cell biology: adhesion articulatedA Paul Mould
Nature 432:27-8. 2004 - Integrin alphavbeta1 is a receptor for foot-and-mouth disease virusTerry Jackson
Department of Molecular Biology, Institute for Animal Health, Pirbright, Surrey GU24 ONF, UK
J Virol 76:935-41. 2002..In addition, data are presented suggesting that amino acid residues near the RGD motif may be important for differentiating between the binding specificities of alphavbeta1 and alphavbeta6... - The collagens of hydra provide insight into the evolution of metazoan extracellular matricesXiaoming Zhang
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
J Biol Chem 282:6792-802. 2007....