Research Topics
Species | J R MastersSummaryAffiliation: University College London Country: UK Publications
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Detail Information
Publications
HeLa cells 50 years on: the good, the bad and the uglyJohn R Masters
Institute of Urology, University College London, UK
Nat Rev Cancer 2:315-9. 2002- Proteomics in the analysis of prostate cancerSoren Naaby-Hansen
Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, Royal Free and University College London Medical School, London, UK
Methods Mol Med 81:277-97. 2003 - A PAK4-LIMK1 pathway drives prostate cancer cell migration downstream of HGFTasneem Ahmed
Randall Division of Cell and Molecular Biophysics, King s College London, UK
Cell Signal 20:1320-8. 2008..It is well established that unphosphorylated (active) cofilin is a required to drive cell migration. Our results support a model whereby HGF-stimulated cell migration also requires a cofilin phosphorylation step that is mediated by PAK4... - Prostate cancer proteomicsJohn R Masters
Prostate Cancer Research Centre, UCL, London, United Kingdom
OMICS 15:169-71. 2011..Despite the promise, protoemics has yielded little of relevance to the management of prostate cancer, and most of the work that has been published is either irreproducible or of no clinical value... - Can p53 staining be used to identify patients with aggressive superficial bladder cancer?John R W Masters
Institute of Urology, University College London, 3rd Floor, 67 Riding House Street, London W1W 7EJ, UK
J Pathol 200:74-81. 2003.... - Short tandem repeat profiling provides an international reference standard for human cell linesJ R Masters
Institute of Urology, University College London, 3rd Floor Research Laboratories, 67 Riding House Street, London W1W 7EY, United Kingdom
Proc Natl Acad Sci U S A 98:8012-7. 2001..If DNA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated... - Human cancer cell lines: fact and fantasyJ R Masters
Institute of Urology, University College London, 67 Riding House Street, London W1W 7EY, UK
Nat Rev Mol Cell Biol 1:233-6. 2000..Under the right conditions, and with appropriate controls, properly authenticated cancer cell lines retain the properties of the cancers of origin... - Curing metastatic cancer: lessons from testicular germ-cell tumoursJohn R W Masters
The Prostate Cancer Research Centre, Institute of Urology, University College London, 3rd Floor, 67 Riding House Street, London W1W 7EJ, UK
Nat Rev Cancer 3:517-25. 2003..Why are TGCTs more sensitive to chemotherapeutics than most other tumour types? Answers to this question could lead to new treatments for metastatic cancers... - Clinical applications of expression profiling and proteomics in prostate cancerJohn R W Masters
The Prostate Cancer Research Centre, University College London, London, UK
Anticancer Res 27:1273-6. 2007..In the future, small custom-built chips will be used to detect a small panel of RNA or protein markers to answer specific questions concerning patient management for each type of cancer... - Changing medium and passaging cell linesJohn R Masters
Department of Surgery, Prostate Cancer Research Centre, Royal Free and University College London Medical School, London, UK
Nat Protoc 2:2276-84. 2007..Given the necessary care and attention, most cell lines are easy to maintain and grow... - Prostate cancer stem cell therapy: hype or hope?J R Masters
Department of Surgery, Prostate Cancer Research Centre, University College London, London, UK
Prostate Cancer Prostatic Dis 11:316-9. 2008..Prostate cancer stem cell therapy is a valid goal to aim for, but there are massive hurdles to overcome, even if the concept is shown to be correct... - False cell lines: The problem and a solutionJohn R Masters
Prostate Cancer Research Centre, UCL, London, U K E mail
Cytotechnology 39:69-74. 2002..This review describes how cross-contamination occurs, catalogues the use of false cell lines in leading biomedical journals, and suggests ways to resolve the problem... - Hypomethylation of WNT5A, CRIP1 and S100P in prostate cancerQ Wang
Prostate Cancer Research Centre, Institute of Urology and Nephrology, University College London, and Department of Histopathology, University College London Hospitals Trust, UK
Oncogene 26:6560-5. 2007..Anti-c-Myb antibody co-precipitation with WNT5A was methylation-sensitive in 1542-NPTX cells. It is likely that an epigenetic mechanism regulates WNT5A expression in prostate cancer... - O6-alkylguanine-DNA-alkyltransferase activity and nitrosourea sensitivity in human cancer cell linesM C Walker
Institute of Urology and Nephrology, University College London, UK
Br J Cancer 66:840-3. 1992..These results support the suggestion that resistance to nitrosoureas can be mediated by mechanisms other than ATase and that at relatively high levels of expression, ATase does not confer resistance in proportion to its activity... - Biochemical basis of resistance to chemotherapyJ R Masters
Institute of Urology, University College London, St Pauls Hospital, U K
Radiother Oncol 19:297-305. 1990..Following a general introduction four areas of topical interest are discussed: (1) multidrug resistance and P-glycoprotein, (2) glutathione and its related enzymes, (3) topoisomerase II and (4) DNA repair... - Heat shock protein expression in testis and bladder cancer cell lines exhibiting differential sensitivity to heatE H Richards
Institute of Urology and Nephrology, University College London, UK
Br J Cancer 72:620-6. 1995.... - Epithelial cell differentiation pathways in the human prostate: identification of intermediate phenotypes by keratin expressionD L Hudson
Institute of Urology and Nephrology, Research Laboratories, University College London Medical School, UK
J Histochem Cytochem 49:271-8. 2001..J Histochem Cytochem 49:271-278, 2001).. - Rapid identification of antisense mRNA-expressing clones using strand-specific RT-PCRMichele Cummings
Prostate Cancer Research Centre, University College London, London W1W 7EJ, England
Antisense Nucleic Acid Drug Dev 13:115-7. 2003..This approach allows earlier identification of potentially useful clones and cuts down on the number of clones to be screened by Western blotting... - Genome-wide screening for genetic changes in a matched pair of benign and prostate cancer cell lines using array CGHN Brookman-Amissah
Prostate Cancer Research Centre, Institute of Urology, University College London, London, UK
Prostate Cancer Prostatic Dis 8:335-43. 2005..The aCGH results were compared to gene expression data obtained using DNA microarrays and suggested the involvement of caspases and ICEBERG on 11q and E2F1 on chromosome 20q... - Proliferative heterogeneity in the human prostate: evidence for epithelial stem cellsD L Hudson
Institute of Urology and Nephrology, Research Laboratories, Department of Surgery, University College London Medical School, University of London, United Kingdom
Lab Invest 80:1243-50. 2000..On the basis of their proliferative characteristics and pluripotency, the type II colonies may be the progeny of stem cells and the type I colonies of a more differentiated transit-amplifying population... - Cancer stem cellsJ R Masters
Prostate Cancer Research Centre, Institute of Urology, University College London, London, UK
BJU Int 92:661-2. 2003 - KW-2149 (7-N-[2-[gamma-L-glutamylamino]ethyldithioethyl] mitomycin C): DNA interactions and drug uptake following serum activationS R McAdam
Department of Oncology, University College London, UK
Biochem Pharmacol 55:1777-83. 1998..The results suggest that uptake is passive, and this indicates that a component in serum modifies KW-2149 to a form that passively enters cells more rapidly... - Comparison of marker protein expression in benign prostatic hyperplasia in vivo and in vitroP M Fry
Institute of Urology, Royal Free and University College London Medical School, London, UK
BJU Int 85:504-13. 2000..Co-localization of proteins was compared in frozen-tissue sections and cultured cells by simultaneous multiple immunofluorescence, and recorded using a high-resolution charge-coupled device camera... - Differential protein synthesis and expression levels in normal and neoplastic human prostate cells and their regulation by type I and II interferonsKohji Nagano
Ludwig Institute for Cancer Research, Royal Free and University College London Medical School, London, UK
Oncogene 23:1693-703. 2004.... - Plexin-B1 mutations in prostate cancerOscar Gee Wan Wong
Prostate Cancer Research Centre, Institute of Urology, University College London, 67 Riding House Street, London W1W 7EJ, United Kingdom
Proc Natl Acad Sci U S A 104:19040-5. 2007..These results identify a key role for Plexin-B1 and the semaphorin signaling pathway it mediates in prostate cancer... - XPA versus ERCC1 as chemosensitising agents to cisplatin and mitomycin C in prostate cancer cells: role of ERCC1 in homologous recombination repairMichele Cummings
Prostate Cancer Research Centre, Institute of Urology, University College London, and St Bartholomew s Hospital, Department of Medical Oncology, London W1W 7EJ, UK
Biochem Pharmacol 72:166-75. 2006..These results indicate that ERCC1 is a broader therapeutic target than XPA with which to sensitise cancer cells to chemotherapy because of its additional role in recombination repair... - Regulation of DNA repair gene expression in human cancer cell linesClaire J McGurk
Prostate Cancer Research Centre, Institute of Urology, UCL, 3rd Floor Research Laboratories, London, W1W 7EJ, United Kingdom
J Cell Biochem 97:1121-36. 2006..Taken together, these results suggest that constitutive levels of these DNA repair proteins are controlled at the level of translation... - Combined affinity labelling and mass spectrometry analysis of differential cell surface protein expression in normal and prostate cancer cellsClaire Hastie
Prostate Cancer Research Centre, Royal Free and University College London Medical School, UK
Oncogene 24:5905-13. 2005.... - Differential expression of CD44 during human prostate epithelial cell differentiationTahirah N Alam
Prostate Cancer Research Centre, Institute of Urology, University College London, London, UK
J Histochem Cytochem 52:1083-90. 2004..Therefore, CD44 v3-v10 may be important as a cell surface marker for differentiating cells in the prostate epithelium... - Reduced levels of XPA, ERCC1 and XPF DNA repair proteins in testis tumor cell linesCarey Welsh
University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA 15213 1863, USA
Int J Cancer 110:352-61. 2004..Our results encourage further investigation of the possibility that low levels of these nucleotide excision repair proteins could be related to the favorable response of testis tumors to cisplatin-based chemotherapy... - Tumour markers for managing men who present with metastatic prostate cancer and serum prostate-specific antigen levels of <10 ng/mLAlison J Birtle
The Prostate Cancer Research Centre, The Institute of Urology, London, UK
BJU Int 96:303-7. 2005..There was strong AR expression in 30 (91%) cases and it was present in areas where PSA was absent. CONCLUSION: In this patient group, immunohistochemical assessments of PSMA and AR are potentially useful as diagnostic markers... - Clinical features of patients who present with metastatic prostate carcinoma and serum prostate-specific antigen (PSA) levels < 10 ng/mL: the "PSA negative" patientsAlison J Birtle
Prostate Cancer Research Center, The Institute of Urology, University College London, London, United Kingdom
Cancer 98:2362-7. 2003..To the authors' knowledge, little information exists in the literature regarding patterns of disease and response to treatment. The authors wished to define the clinical features of this patient group... - Prostate epithelial stem cell isolation and cultureDavid L Hudson
Prostate Cancer Research Centre, Royal Free and University College London Medical School, London, UK
Methods Mol Med 81:59-67. 2003 - Elevation of XPA protein level in testis tumor cells without increasing resistance to cisplatin or UV radiationBeate Köberle
Department of Pharmacology, University of Pittsburgh Medical School and University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania, USA
Mol Carcinog 47:580-6. 2008..The relative sensitivity of testis tumor cells to cisplatin, UV radiation, and other DNA damaging agents is likely related not to NER capacity, but to other factors such as the integrity of the p53 pathway in these cells...