A G Lee

Summary

Affiliation: University of Southampton
Country: UK

Publications

  1. ncbi Lipid sorting: lipids do it on their own
    Anthony G Lee
    School of Biological Sciences, University of Southampton, UK
    Curr Biol 15:R421-3. 2005
  2. ncbi Lipid-protein interactions in biological membranes: a structural perspective
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton, UK
    Biochim Biophys Acta 1612:1-40. 2003
  3. ncbi Ca2+ -ATPase structure in the E1 and E2 conformations: mechanism, helix-helix and helix-lipid interactions
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Biochim Biophys Acta 1565:246-66. 2002
  4. ncbi How phospholamban could affect the apparent affinity of Ca(2+)-ATPase for Ca(2+) in kinetic experiments
    Anthony G Lee
    School of Biological Sciences, University of Southampton, SO16 7PX Southampton, UK
    FEBS Lett 551:37-41. 2003
  5. ncbi The effects of lipids on channel function
    Anthony G Lee
    School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, UK
    J Biol 8:86. 2009
  6. ncbi A calcium pump made visible
    Anthony G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, UK
    Curr Opin Struct Biol 12:547-54. 2002
  7. ncbi Lipid-protein interactions
    Anthony G Lee
    School of Biological Sciences, Life Sciences Building, University of Southampton, U K
    Biochem Soc Trans 39:761-6. 2011
  8. ncbi The potassium channel KcsA and its interaction with the lipid bilayer
    I M Williamson
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Cell Mol Life Sci 60:1581-90. 2003
  9. ncbi Biological membranes: the importance of molecular detail
    Anthony G Lee
    School of Biological Sciences, Life Sciences Building 85, University of Southampton, Southampton, SO17 1BJ, UK
    Trends Biochem Sci 36:493-500. 2011
  10. ncbi How lipids affect the activities of integral membrane proteins
    Anthony G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochim Biophys Acta 1666:62-87. 2004

Collaborators

  • J M East
  • P J Lockyer
  • P Adams
  • Mairi E Sandison
  • Michele Zagnoni
  • Javier Palacios
  • Derek Marsh
  • Andrew M Powl
  • Phedra Marius
  • Joanne Carney
  • Simon J Alvis
  • Ian M Williamson
  • Sanjay Mall
  • John Butler
  • J D Pilot
  • Jiraphun Jittikoon
  • J Neville Wright
  • A M Powl
  • Robert Broadbridge
  • S M Twine
  • Ashish Arora
  • Elizabeth H Clark
  • Thomas Newton
  • I M Williamson
  • Melanie J Logan-Smith
  • Wendy S Smith
  • M J Logan-Smith
  • S Mall
  • Hywel Morgan
  • Neil A Hanley
  • David I Wilson
  • Cosma Spalluto
  • Steven L Harrison
  • Michael G Gore
  • P Marius
  • J Carney
  • P Morton
  • S J Alvis
  • B C Fish
  • M G Gore
  • John Chad
  • John P J Black
  • R P Sharma
  • R Broadbridge
  • G W Gould
  • J M McWhirter

Detail Information

Publications45

  1. ncbi Lipid sorting: lipids do it on their own
    Anthony G Lee
    School of Biological Sciences, University of Southampton, UK
    Curr Biol 15:R421-3. 2005
    ..How are lipid molecules sorted between organelles in eukaryotic cells? A recent paper shows that the work needed to bend a membrane and form a vesicle is sufficient to sort lipid molecules...
  2. ncbi Lipid-protein interactions in biological membranes: a structural perspective
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton, UK
    Biochim Biophys Acta 1612:1-40. 2003
    ..The function of membrane proteins is dependent on the thickness of the surrounding lipid bilayer, sometimes on the presence of specific, usually anionic, phospholipids, and sometimes on the phase of the phospholipid...
  3. ncbi Ca2+ -ATPase structure in the E1 and E2 conformations: mechanism, helix-helix and helix-lipid interactions
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK
    Biochim Biophys Acta 1565:246-66. 2002
    ..It is suggested that this and other similar clefts provide binding sites for a variety of hydrophobic molecules affecting the activity of the Ca(2+)-ATPase...
  4. ncbi How phospholamban could affect the apparent affinity of Ca(2+)-ATPase for Ca(2+) in kinetic experiments
    Anthony G Lee
    School of Biological Sciences, University of Southampton, SO16 7PX Southampton, UK
    FEBS Lett 551:37-41. 2003
    ..It is shown that this can be explained by a scheme in which the ATPase shows two modes of binding for PLN, one of high and one of low affinity; the proposed scheme is not dependent on the kinetic model assumed for the Ca(2+)-ATPase...
  5. ncbi The effects of lipids on channel function
    Anthony G Lee
    School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, UK
    J Biol 8:86. 2009
    ..Anionic lipids affect the function of many channels, including connexins, as shown in a recent report in BMC Biology. These effects might follow from direct binding of the anionic lipids to the channels...
  6. ncbi A calcium pump made visible
    Anthony G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, UK
    Curr Opin Struct Biol 12:547-54. 2002
    ..The Ca(2+)-ATPase is implicated in thermogenesis in some types of muscle; this could involve processes of slippage and leak modulated by interaction between the Ca(2+)-ATPase and sarcolipin...
  7. ncbi Lipid-protein interactions
    Anthony G Lee
    School of Biological Sciences, Life Sciences Building, University of Southampton, U K
    Biochem Soc Trans 39:761-6. 2011
    ....
  8. ncbi The potassium channel KcsA and its interaction with the lipid bilayer
    I M Williamson
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Cell Mol Life Sci 60:1581-90. 2003
    ....
  9. ncbi Biological membranes: the importance of molecular detail
    Anthony G Lee
    School of Biological Sciences, Life Sciences Building 85, University of Southampton, Southampton, SO17 1BJ, UK
    Trends Biochem Sci 36:493-500. 2011
    ....
  10. ncbi How lipids affect the activities of integral membrane proteins
    Anthony G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochim Biophys Acta 1666:62-87. 2004
    ..Finally, the role of non-annular, or 'co-factor' lipids, tightly bound to membrane proteins, is described...
  11. ncbi What the structure of a calcium pump tells us about its mechanism
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochem J 356:665-83. 2001
    ....
  12. ncbi How lipids and proteins interact in a membrane: a molecular approach
    Anthony G Lee
    School of Biological Sciences, University of Southampton, Southampton, UK
    Mol Biosyst 1:203-12. 2005
    ..Evidence is also accumulating for the presence of 'co-factor' lipid molecules binding with high specificity to membrane proteins, often between transmembrane alpha-helices, and often being essential for activity...
  13. ncbi How lipids interact with an intrinsic membrane protein: the case of the calcium pump
    A G Lee
    Department of Biochemistry, University of Southampton, Southampton SO16 7PX, UK
    Biochim Biophys Acta 1376:381-90. 1998
    ..The changes in activity are likely to follow from tilting of trans-membrane alpha-helices in the ATPase. In simple model systems it has been shown that the extent to which lipids can distort to match the protein is limited...
  14. ncbi Curcumin, a molecule that inhibits the Ca2+-ATPase of sarcoplasmic reticulum but increases the rate of accumulation of Ca2+
    M J Logan-Smith
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    J Biol Chem 276:46905-11. 2001
    ....
  15. ncbi Uptake of Ca2+ mediated by the (Ca2+ + Mg2+)-ATPase in reconstituted vesicles
    G W Gould
    Department of Biochemistry, University of Southampton, U K
    Biochim Biophys Acta 904:36-44. 1987
    ..It is suggested that the effect of phosphatidylethanolamine on the level of Ca2+ accumulation follows from an effect on the rate of Ca2+ efflux mediated by the ATPase...
  16. ncbi Effects of bilayer thickness on the activity of diacylglycerol kinase of Escherichia coli
    J D Pilot
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX
    Biochemistry 40:8188-95. 2001
    ..Cholesterol increased the activity in di(C14:1)PC but slightly decreased it in di(C18:1)PC or di(C24:1)PC, effects that could follow from changes in bilayer thickness caused by cholesterol...
  17. ncbi Mutational analysis of trans-membrane helices M3, M4, M5 and M7 of the fast-twitch Ca2+-ATPase
    P Adams
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
    Biochem J 335:131-8. 1998
    ....
  18. ncbi Lipid interactions with bacterial channels: fluorescence studies
    A M Powl
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochem Soc Trans 33:905-9. 2005
    ..Fluorescence quenching methods can also be used to study binding of lipids to non-annular sites such as those between monomers in the homotetrameric potassium channel KcsA...
  19. ncbi Self-association of model transmembrane alpha-helices is modulated by lipid structure
    S Mall
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochemistry 40:12379-86. 2001
    ..Addition of cholesterol to form the liquid-ordered state led to increased dimerization but without phase separation. The presence of phosphatidylethanolamine had little effect on dimerization...
  20. ncbi Mechanism of binding of warfarin enantiomers to recombinant domains of human albumin
    S M Twine
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, UK
    Arch Biochem Biophys 414:83-90. 2003
    ..These preferences can largely be explained by the domains having different rates for step 2 of the binding process...
  21. ncbi Effects of phospholipid headgroup and phase on the activity of diacylglycerol kinase of Escherichia coli
    J D Pilot
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX
    Biochemistry 40:14891-7. 2001
    ..These experiments suggest that the presence of lipids preferring a hexagonal H(II) phase leads to low activities. Activities of DGK are low in a gel phase lipid...
  22. ncbi Myelin: Delivery by raft
    A G Lee
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, SO16 7PX, Southampton, UK
    Curr Biol 11:R60-2. 2001
    ..The major intrinsic membrane protein of myelin, proteolipid protein, interacts with rafts in oligodendrocytes but not with the different rafts found in other cell types...
  23. ncbi Controlled delivery of proteins into bilayer lipid membranes on chip
    Michele Zagnoni
    NSI Group, School of Electronics and Computer Science, University of Southampton, Highfield, Southampton, UK
    Lab Chip 7:1176-83. 2007
    ..Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system...
  24. ncbi Cell biology: a greasy grip
    Anthony G Lee
    Nature 438:569-70. 2005
  25. ncbi The presence of sarcolipin results in increased heat production by Ca(2+)-ATPase
    Sanjay Mall
    School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, United Kingdom
    J Biol Chem 281:36597-602. 2006
    ..9 x 10(-4) +/- 2.9 x 10(-4) in units of mol fraction per monolayer. It is suggested that the interaction between Ca(2+)-ATPase and SLN in the sarcoplasmic reticulum could be important in thermogenesis by the sarcoplasmic reticulum...
  26. ncbi Phospholamban and sarcolipin are maintained in the endoplasmic reticulum by retrieval from the ER-Golgi intermediate compartment
    John Butler
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Cardiovasc Res 74:114-23. 2007
    ..We have examined the hypothesis that phospholamban and sarcolipin are maintained in the SR by a process of retrieval from post-SR compartments and the role of their transmembrane domains in targeting...
  27. ncbi Penetration of lipid chains into transmembrane surfaces of membrane proteins: studies with MscL
    Joanne Carney
    School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, United Kingdom
    Biophys J 92:3556-63. 2007
    ....
  28. ncbi Ion channels: a paddle in oil
    Anthony G Lee
    Nature 444:697. 2006
  29. ncbi Different effects of lipid chain length on the two sides of a membrane and the lipid annulus of MscL
    Andrew M Powl
    School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Biophys J 93:113-22. 2007
    ....
  30. ncbi Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening
    Phedra Marius
    School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Biophys J 94:1689-98. 2008
    ..The conductance of the open channel increases with increasing concentration of anionic lipid, an effect possibly due to effects of anionic lipid on the concentration of K(+) close to the membrane surface...
  31. ncbi A fluorescence method to define transmembrane alpha-helices in membrane proteins: studies with bacterial diacylglycerol kinase
    Jiraphun Jittikoon
    School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochemistry 46:10950-9. 2007
    ....
  32. ncbi The interfacial lipid binding site on the potassium channel KcsA is specific for anionic phospholipids
    Phedra Marius
    School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Biophys J 89:4081-9. 2005
    ..It is suggested that this pattern of binding follows from the pattern of charge distribution on KcsA on the intracellular side of the membrane...
  33. ncbi Evidence for a global inhibitor-induced conformation change on the Ca(2+)-ATPase of sarcoplasmic reticulum from paired inhibitor studies
    Melanie J Logan-Smith
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, U.K
    Biochemistry 41:2869-75. 2002
    ....
  34. ncbi Interactions of phospholipids with the potassium channel KcsA
    Ian M Williamson
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, United Kingdom
    Biophys J 83:2026-38. 2002
    ..In the chain length range C18 to C24 it is suggested that the Trp residues become more buried within the hydrocarbon core of the bilayer...
  35. ncbi Lipid-protein interactions with cardiac phospholamban studied by spin-label electron spin resonance
    Ashish Arora
    , Abt. Spektroskopie, , Germany
    Biochemistry 42:5151-8. 2003
    ..A similar pattern of lipid selectivity is obtained in DMPG, but the absolute selectivities are reduced considerably. The results are discussed in terms of the integration of the regulatory species in the lipid membrane...
  36. ncbi The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase
    Elizabeth H Clark
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biochemistry 42:11065-73. 2003
    ..As well as any structural effects, the presence of Trp residues in DGK has a clear effect on thermal stability...
  37. ncbi Lipid-protein interactions studied by introduction of a tryptophan residue: the mechanosensitive channel MscL
    Andrew M Powl
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochemistry 42:14306-17. 2003
    ..Quenching experiments suggest two classes of lipid binding sites on TbMscL for phosphatidic acid, binding of phosphatidic acid being much less dependent on ionic strength than binding of phosphatidylserine...
  38. ncbi Interactions of anionic phospholipids and phosphatidylethanolamine with the potassium channel KcsA
    Simon J Alvis
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton, United Kingdom
    Biophys J 85:3828-38. 2003
    ....
  39. ncbi Ca2+ transport by the synaptosomal plasma membrane Ca2+-ATPase and the effect of thioridazine
    Javier Palacios
    , Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Biochemistry 43:2353-8. 2004
    ..The higher levels of Ca(2+) accumulation obtained in the presence of thioridazine could be explained by a reduction of the rate of slippage on the plasma membrane ATPase...
  40. ncbi Identification of the hydrophobic thickness of a membrane protein using fluorescence spectroscopy: studies with the mechanosensitive channel MscL
    Andrew M Powl
    School of Biological Sciences, University of Southampton, UK
    Biochemistry 44:5713-21. 2005
    ....
  41. ncbi Heterogeneity in the binding of lipid molecules to the surface of a membrane protein: hot spots for anionic lipids on the mechanosensitive channel of large conductance MscL and effects on conformation
    Andrew M Powl
    School of Biological Sciences, University of Southampton, UK
    Biochemistry 44:5873-83. 2005
    ....
  42. ncbi Sarcolipin uncouples hydrolysis of ATP from accumulation of Ca2+ by the Ca2+-ATPase of skeletal-muscle sarcoplasmic reticulum
    Wendy S Smith
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochem J 361:277-86. 2002
    ..It is suggested that the presence of SLN could be important in non-shivering thermogenesis, a process in which heat is generated by hydrolysis of ATP by skeletal-muscle sarcoplasmic reticulum...
  43. ncbi Sarco/endoplasmic-reticulum calcium ATPase SERCA1 is maintained in the endoplasmic reticulum by a retrieval signal located between residues 1 and 211
    Thomas Newton
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
    Biochem J 371:775-82. 2003
    ..These results indicate that the N-terminal region of SERCA1, containing transmembrane helices M1 and M2, contains an ER-retrieval signal...
  44. ncbi Fluorescence quenching methods to study lipid-protein interactions
    Joanne Carney
    University of Southampton, Southampton, United Kingdom
    Curr Protoc Protein Sci . 2006
    ..Details are included on data analysis, including equations and software that can be used for fitting the fluorescence quenching data...
  45. ncbi Synaptic vesicle fusion and synaptotagmin: 2B or not 2B?
    Anthony G Lee
    Nat Neurosci 5:823-4. 2002