Achillefs N Kapanidis

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. ncbi Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation
    Nam Ki Lee
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA
    Biophys J 88:2939-53. 2005
  2. ncbi Defining the limits of single-molecule FRET resolution in TIRF microscopy
    Seamus J Holden
    Biological Physics Research Group, Department of Physics, University of Oxford, Oxford, United Kingdom
    Biophys J 99:3102-11. 2010
  3. ncbi Alternating-laser excitation of single molecules
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095 1569, USA
    Acc Chem Res 38:523-33. 2005
  4. ncbi Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Science 314:1144-7. 2006
  5. ncbi Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry, and California Nanosystems Institute, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Mol Cell 20:347-56. 2005
  6. ncbi Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes
    Emmanuel Margeat
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA
    Biophys J 90:1419-31. 2006
  7. ncbi Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 101:8936-41. 2004
  8. ncbi Probing biomolecular structures and dynamics of single molecules using in-gel alternating-laser excitation
    Yusdi Santoso
    Department of Physics and Biological Physics Research Group, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, OX1 3PU, United Kingdom
    Anal Chem 81:9561-70. 2009
  9. ncbi Surfing on a new wave of single-molecule fluorescence methods
    Johannes Hohlbein
    Department of Physics, Biological Physics Research Group, University of Oxford, Oxford, UK
    Phys Biol 7:031001. 2010
  10. ncbi Biology, one molecule at a time
    Achillefs N Kapanidis
    Department of Physics and Interdisciplinary Research Collaboration in Bionanotechnology, Clarendon Laboratory, University of Oxford, Oxford, UK
    Trends Biochem Sci 34:234-43. 2009

Collaborators

Detail Information

Publications17

  1. ncbi Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation
    Nam Ki Lee
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA
    Biophys J 88:2939-53. 2005
    ..ALEX can benefit structural analysis of biomolecules, especially when such molecules are inaccessible to conventional structural methods due to heterogeneity or transient nature...
  2. ncbi Defining the limits of single-molecule FRET resolution in TIRF microscopy
    Seamus J Holden
    Biological Physics Research Group, Department of Physics, University of Oxford, Oxford, United Kingdom
    Biophys J 99:3102-11. 2010
    ..the mechanisms of which are often characterized by distance changes on the scale of one DNA basepair...
  3. ncbi Alternating-laser excitation of single molecules
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095 1569, USA
    Acc Chem Res 38:523-33. 2005
    ..The principle of alternation is compatible with several time scales, allowing monitoring of fast dynamics or simultaneous monitoring of a large number of individual molecules...
  4. ncbi Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Science 314:1144-7. 2006
    ....
  5. ncbi Retention of transcription initiation factor sigma70 in transcription elongation: single-molecule analysis
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry, and California Nanosystems Institute, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Mol Cell 20:347-56. 2005
    ....
  6. ncbi Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes
    Emmanuel Margeat
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA
    Biophys J 90:1419-31. 2006
    ....
  7. ncbi Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules
    Achillefs N Kapanidis
    Department of Chemistry and Biochemistry, University of California, 607 Charles E Young Drive East, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 101:8936-41. 2004
    ....
  8. ncbi Probing biomolecular structures and dynamics of single molecules using in-gel alternating-laser excitation
    Yusdi Santoso
    Department of Physics and Biological Physics Research Group, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, OX1 3PU, United Kingdom
    Anal Chem 81:9561-70. 2009
    ..In-gel ALEX will be a versatile tool for studying structures and dynamics of complex biomolecules and their assemblies...
  9. ncbi Surfing on a new wave of single-molecule fluorescence methods
    Johannes Hohlbein
    Department of Physics, Biological Physics Research Group, University of Oxford, Oxford, UK
    Phys Biol 7:031001. 2010
    ....
  10. ncbi Biology, one molecule at a time
    Achillefs N Kapanidis
    Department of Physics and Interdisciplinary Research Collaboration in Bionanotechnology, Clarendon Laboratory, University of Oxford, Oxford, UK
    Trends Biochem Sci 34:234-43. 2009
    ..These approaches have been instrumental in addressing important biological questions in diverse areas such as protein-DNA interactions, protein folding and the function(s) of membrane proteins...
  11. ncbi The power and prospects of fluorescence microscopies and spectroscopies
    Xavier Michalet
    Department of Chemistry and Biochemistry, UCLA, Young Hall, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
    Annu Rev Biophys Biomol Struct 32:161-82. 2003
    ....
  12. ncbi Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances
    Nam Ki Lee
    School of Chemistry, Seoul National University, Seoul, Korea
    Biophys J 92:303-12. 2007
    ..This study paves the way for advanced single-molecule analysis of complex mixtures and biomolecular machinery...
  13. ncbi Fluorescence resonance energy transfer (FRET) in analysis of transcription-complex structure and function
    Jayanta Mukhopadhyay
    Waksman Institute, Howard Hughes Medical Institute, Rutgers University, 190 Frelinghuysen Road, Piscataway, New Jersey 08854, USA
    Methods Enzymol 371:144-59. 2003
  14. ncbi Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex
    Vladimir Mekler
    Howard Hughes Medical Institute, Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
    Cell 108:599-614. 2002
    ..1 is located outside the active center cleft. The approach described here should be applicable to the analysis of other nanometer-scale complexes...
  15. ncbi Red light, green light: probing single molecules using alternating-laser excitation
    Yusdi Santoso
    Department of Physics and Interdisciplinary Research Collaboration in Bionanotechnology, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, UK
    Biochem Soc Trans 36:738-44. 2008
    ..We also highlight applications of ALEX to the study of protein-nucleic acid interactions...
  16. ncbi Reconfigurable, braced, three-dimensional DNA nanostructures
    Russell P Goodman
    Nat Nanotechnol 3:93-6. 2008
    ..The introduction of shape-changing structural modules opens new avenues for the manipulation of matter on the nanometre scale...
  17. ncbi Periodic acceptor excitation spectroscopy of single molecules
    Soren Doose
    Clarendon Laboratory, Department of Physics and IRC in Bionanotechnology, Oxford University, Oxford, UK
    Eur Biophys J 36:669-74. 2007
    ....