Research Topics
Species | Robin IrvineSummaryAffiliation: University of Cambridge Country: UK Publications
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Publications
Mobility of proteins associated with the plasma membrane by interaction with inositol lipidsDavid Brough
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
J Cell Sci 118:3019-25. 2005..Moreover, the mobility of GAP1(IP4BP) is not detectably altered by the generation of either of its two potential regulators, Ins(1,3,4,5)P(4) or PtdIns(3,4,5)P(3)...
Back in the water: the return of the inositol phosphatesR F Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1QJ, UK
Nat Rev Mol Cell Biol 2:327-38. 2001..Old and new data point to a new vista of inositol phosphates, with functions in many diverse aspects of cell biology, such as ion-channel physiology, membrane dynamics and nuclear signalling...
20 years of Ins(1,4,5)P3, and 40 years beforeRobin F Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1QJ, UK
Nat Rev Mol Cell Biol 4:586-90. 2003..The background to this discovery is a complex story that goes back more than 50 years and involves a large cast of characters, both chemical and human...
The intracellular localisation and mobility of Type Igamma phosphatidylinositol 4P 5-kinase splice variantsMaria-Luisa Giudici
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
FEBS Lett 580:6933-7. 2006..Moreover, the markedly different localisation and mobility of active versus inactive PIPkin Igamma93 provide insights into the factors that dictate cellular targeting of Type Igamma PIPkins...
Genomic tagging of endogenous type IIbeta phosphatidylinositol 5-phosphate 4-kinase in DT40 cells reveals a nuclear localisationJonathan P Richardson
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
Cell Signal 19:1309-14. 2007..Genomic tagging of endogenous proteins in DT40 cells is a technique that offers unique advantages in studying endogenous signalling proteins...
Phosphatidylinositol 4-phosphate 5-kinase I?_v6, a new splice variant found in rodents and humansYang Xia
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1PD, UK
Biochem Biophys Res Commun 411:416-20. 2011....
Cell signaling. The art of the solubleRobin Irvine
Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, UK
Science 316:845-6. 2007
The regulation and function of inositol 1,4,5-trisphosphate 3-kinasesRobin F Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1PD, UK
Adv Enzyme Regul 46:314-23. 2006
Nuclear inositide signalling -- expansion, structures and clarificationRobin F Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1PD, UK
Biochim Biophys Acta 1761:505-8. 2006..Here, a few key issues with regard to nuclear inositide signalling are briefly addressed...
Inositol phospholipids: translocation, translocation, translocationR Irvine
Department of Pharmacology University of Cambridge Tennis Court Road, Cambridge CB2 1QJ, UK
Curr Biol 8:R557-9. 1998....
Inositide evolution - towards turtle domination?Robin F Irvine
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
J Physiol 566:295-300. 2005..This review will focus on two specific aspects of this diversity: the evolution of the polyphosphoinositides, and the synthesis and functions of the higher inositol phosphates...
Nuclear lipid signallingRobin F Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1QJ, UK
Nat Rev Mol Cell Biol 4:349-60. 2003..However, exactly as has been discovered in the cytoplasm, this is just part of a complex picture that involves many other lipids and functions...
Nuclear lipid signalingR Irvine
Department of Pharmacology, University of Cambridge, Cambridge, UK
Sci STKE 2000:re1. 2000....
Manganese-stimulated phosphatidylinositol headgroup exchange in rat liver microsomesR F Irvine
Department of Pharmacology, University of Cambridge, UK
Biochim Biophys Acta 1393:292-8. 1998....
Inositol 1,3,4,5-tetrakisphosphate as a second messenger--a special role in neurones?R F Irvine
Department of Pharmacology, University of Cambridge, UK
Chem Phys Lipids 98:49-57. 1999..In this review we discuss the possible relevance of these high expression levels to the complex way in which neurones control Ca2+ and use it as a second messenger...
Inositol phosphates: Does IP(4) run a protection racket?R Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1QJ, UK
Curr Biol 11:R172-4. 2001..Recent findings about a hitherto unsuspected action of the IP(3) 3-kinase product, IP(4), suggest that the evolution of IP(3) 3-kinase may have even more far-reaching consequences than we thought...
Nuclear lipid signalingRobin F Irvine
Department of Pharmacology, University of Cambridge, Cambridge CB2 1QJ, UK
Sci STKE 2002:re13. 2002....
Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brainJonathan H Clarke
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD United Kingdom
J Comp Neurol 517:296-312. 2009..Overall, our data suggest that PIP4Kgamma may have a role in neuron function, specifically in the regulation of vesicular transport, in specific regions of the developed brain...
Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephronJonathan H Clarke
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
Am J Physiol Renal Physiol 295:F1422-30. 2008..Overall our data suggest that PIP4Kgamma may have a function in the regulation of vesicular transport in specialized kidney epithelial cells...
Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to coordinate free movement with spatial informationGerald R V Hammond
Department of Pharmacology, University of Cambridge, Cambridge, England, UK
J Cell Biol 184:297-308. 2009....
Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P(2)Gerald R V Hammond
Department of Pharmacology, University of Cambridge, Cambridge, UK
Biochem J 422:23-35. 2009..In addition, we present evidence that the majority of PtdIns4P resides in the plasma membrane, where it is metabolically distinct from the steady-state plasma membrane pool of PtdIns(4,5)P(2)...
Type IIalpha phosphatidylinositol phosphate kinase associates with the plasma membrane via interaction with type I isoformsKatherine A Hinchliffe
University of Cambridge, Department of Pharmacology, Tennis Court Road, Cambridge, CB2 1PD, U K
Biochem J 363:563-70. 2002..This change in subcellular localization could result in improved access of the type IIalpha PIPkin to its lipid substrates...
Regulation of type II PIP kinase by PKD phosphorylationKatherine A Hinchliffe
Faculty of Life Sciences, The University of Manchester, Core Technology Facility, 46 Grafton Street, Manchester M13 9NT, United Kingdom
Cell Signal 18:1906-13. 2006..We conclude that the type II PIP kinases are physiological targets for PKD phosphorylation, and that this modification is likely to regulate inositol lipid turnover by inhibition of these lipid kinases...
Regulation of inositol 1,4,5-trisphosphate 3-kinases by calcium and localization in cellsSamantha M Lloyd-Burton
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
J Biol Chem 282:9526-35. 2007....
Inositol lipids: to PHix or not to PHix?Robin Irvine
Department of Pharmacology, Tennis Court Road, Cambridge CB2 1PD, UK
Curr Biol 14:R308-10. 2004
A novel neuronal-specific splice variant of Type I phosphatidylinositol 4-phosphate 5-kinase isoform gammaMaria Luisa Giudici
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
Biochem J 379:489-96. 2004....
Effects of lipid kinase expression and cellular stimuli on phosphatidylinositol 5-phosphate levels in mammalian cell linesHilary F Roberts
Department of Pharmacology, University of Cambridge, UK
FEBS Lett 579:2868-72. 2005....
The structural integrity of the endoplasmic reticulum, and its possible regulation by inositol 1,3,4,5-tetrakisphosphateDavid Brough
Department of Pharmacology, Cambridge, UK
Cell Calcium 38:153-9. 2005..However, we were subsequently unable to detect by FRAP any significant effect of Ins(1,3,4,5)P(4) on ER integrity...
Genomic tagging reveals a random association of endogenous PtdIns5P 4-kinases IIalpha and IIbeta and a partial nuclear localization of the IIalpha isoformMinchuan Wang
Department of Pharmacology, Tennis Court Road, Cambridge, U K
Biochem J 430:215-21. 2010..Overall the results suggest a function of PtdIns5P 4-kinase IIbeta may be to target the more active IIalpha isoform into the nucleus...
Do mammals make all their own inositol hexakisphosphate?Andrew J Letcher
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB21PD, UK
Biochem J 416:263-70. 2008..Therefore claims that administrating InsP6 in the diet or applying it topically can produce health benefits by increasing extracellular InsP6 levels may require reassessment...
A femtomole-sensitivity mass assay for inositol hexakisphosphateAndrew J Letcher
Department of Pharmacology, University of Cambridge, Cambridge, UK
Methods Mol Biol 645:61-71. 2010..But the assay is unambiguous and is capable of quantifying accurately as little as 10 fmol of InsP(6) in a cell extract...
Calcium-triggered exit of F-actin and IP(3) 3-kinase A from dendritic spines is rapid and reversibleMichael J Schell
Department of Pharmacology, University of Cambridge CB2 1PD, UK
Eur J Neurosci 24:2491-503. 2006..This process contributes to changes in spine F-actin shape and content during synaptic activity, and might also regulate spine IP3 signals...
Solution behaviour of myo-inositol hexakisphosphate in the presence of multivalent cations. Prediction of a neutral pentamagnesium species under cytosolic/nuclear conditionsJulia Torres
, Departamento Estrella Campos, , , CC 1157, Montevideo, Uruguay
J Inorg Biochem 99:828-40. 2005..In vitro, InsP6 also forms high affinity 1:1 complexes with Fe(III) and Al(III). However, our data predict that in the biological context of excess free Mg(II), neither Fe(III) nor Fe(II) are complexed by InsP6...
Structure of a human inositol 1,4,5-trisphosphate 3-kinase: substrate binding reveals why it is not a phosphoinositide 3-kinaseBeatriz Gonzalez
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
Mol Cell 15:689-701. 2004..This lobe embraces all of the phosphates of Ins(1,4,5)P3 in a positively charged pocket, explaining the enzyme's substrate specificity and its inability to phosphorylate PtdIns(4,5)P2, the membrane-resident analog of Ins(1,4,5)P3...
Phospholipase D2 stimulates integrin-mediated adhesion via phosphatidylinositol 4-phosphate 5-kinase Igamma bDale J Powner
CRUK Institute for Cancer Studies, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
J Cell Sci 118:2975-86. 2005....
Kinetic model of the inositol trisphosphate receptor that shows both steady-state and quantal patterns of Ca2+ release from intracellular storesAlan P Dawson
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
Biochem J 370:621-9. 2003....
