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Genomes and Genes | David J HarveySummaryAffiliation: University of Oxford Country: UK Publications
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Publications
Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update for 2007-2008David J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Mass Spectrom Rev 31:183-311. 2012..However, most of the work relating to sample preparation and glycan synthesis is equally relevant to electrospray and, consequently, those proposing analyses by electrospray should also find material in this review of interest...- Fragmentation of negative ions from N-linked carbohydrates, part 4. Fragmentation of complex glycans lacking substitution on the 6-antennaDavid J Harvey
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
J Mass Spectrom 45:528-35. 2010..Glycans obtained from such enzyme-deficient patients can, thus, be a valuable way of obtaining spectra of specific isomers in a relatively pure state for interpretation of mass spectra... - Identification of high-mannose and multiantennary complex-type N-linked glycans containing alpha-galactose epitopes from Nurse shark IgM heavy chainDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Glycoconj J 26:1055-64. 2009..This appears to be the first comprehensive study of glycosylation in this species of animal. The glycosylation pattern has implications for the mechanism of activation of the complement system by nurse shark IgM... - Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update for the period 2005-2006David J Harvey
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
Mass Spectrom Rev 30:1-100. 2011..Large carbohydrate-protein complexes and glycodendrimers are highlighted in this final section... - Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2003-2004David J Harvey
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
Mass Spectrom Rev 28:273-361. 2009.... - Application of negative ion MS/MS to the identification of N-glycans released from carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1)David J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, UK
J Mass Spectrom 44:50-60. 2009..Sialic acids were in both alpha2-3 and alpha2-6 linkage as determined by MALDI-TOF MS following linkage-specific derivatization... - Proposal for a standard system for drawing structural diagrams of N- and O-linked carbohydrates and related compoundsDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, UK
Proteomics 9:3796-801. 2009..Constituent monosaccharides are depicted by shapes modified to show OAc, deoxy, etc. Linkage is indicated by the bond angle and anomericity by solid (beta) or dashed (alpha) lines... - Symbol nomenclature for representing glycan structures: Extension to cover different carbohydrate typesDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX, UK
Proteomics 11:4291-5. 2011..It is hoped that these proposals will stimulate discussion so that a consensus can be reached as to how the glycobiology community can best convey complex information in a simple manner... - Ion mobility mass spectrometry for extracting spectra of N-glycans directly from incubation mixtures following glycan release: application to glycans from engineered glycoforms of intact, folded HIV gp120David J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
J Am Soc Mass Spectrom 22:568-81. 2011.... - Differentiation between isomeric triantennary N-linked glycans by negative ion tandem mass spectrometry and confirmation of glycans containing galactose attached to the bisecting (beta1-4-GlcNAc) residue in N-glycans from IgGDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
Rapid Commun Mass Spectrom 22:1047-52. 2008..Negative ion MS/MS analysis of glycans is likely to prove an invaluable tool in the analysis and monitoring of therapeutic glycoproteins... - Derivatization of carbohydrates for analysis by chromatography; electrophoresis and mass spectrometryDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
J Chromatogr B Analyt Technol Biomed Life Sci 879:1196-225. 2011..All of these groups have been the targets of derivatization and this review attempts to summarise the main methods used for these various functional groups... - Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update covering the period 2001-2002David J Harvey
Department of Biochemistry, Oxford Glycobiology Institute, South Parks Road, Oxford OX1 3QU, UK
Mass Spectrom Rev 27:125-201. 2008..There is an increasing emphasis on its use for examination of biological systems rather than on studies of fundamental aspects and method development and this is reflected by much of the work on applications appearing in tabular form... - Interaction of mannan binding lectin with alpha2 macroglobulin via exposed oligomannose glycans: a conserved feature of the thiol ester protein family?James N Arnold
Medical Research Council Immunochemistry Unit and Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England
J Biol Chem 281:6955-63. 2006..Binding would lead to opsonization or activation of enzyme systems such as complement... - Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectinJames N Arnold
Medical Research Council Immunochemistry Unit, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
J Biol Chem 280:29080-7. 2005..Antigen-bound IgM pentamers therefore do not activate complement via the lectin pathway, but MBL might have a role in the clearance of aggregated IgM... - Inhibition of hybrid- and complex-type glycosylation reveals the presence of the GlcNAc transferase I-independent fucosylation pathwayMax Crispin
Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
Glycobiology 16:748-56. 2006..The presence of the GnT I-independent fucosylation pathway is an important consideration when engineering mammalian glycosylation... - Identification of N-glycans from Ebola virus glycoproteins by matrix-assisted laser desorption/ionisation time-of-flight and negative ion electrospray tandem mass spectrometryGayle Ritchie
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, UK
Rapid Commun Mass Spectrom 24:571-85. 2010..These results confirm and expand previous findings on partial characterisation of the Ebola virus transmembrane glycoprotein. They represent the first detailed data on carbohydrate structures of the Ebola virus sGP... - O-glycan sialylation and the structure of the stalk-like region of the T cell co-receptor CD8Anthony H Merry
Oxford Glycobiology Institute, Department of Biochemistry, The University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
J Biol Chem 278:27119-28. 2003.... - Cellular effects of deoxynojirimycin analogues: inhibition of N-linked oligosaccharide processing and generation of free glucosylated oligosaccharidesHoward R Mellor
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Biochem J 381:867-75. 2004..This represents the most detailed characterization of the FOS structures arising from ER a-glucosidase inhibition to date... - Disruption of alpha-mannosidase processing induces non-canonical hybrid-type glycosylationMax Crispin
Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK
FEBS Lett 581:1963-8. 2007.... - Recovery of intact 2-aminobenzamide-labeled O-glycans released from glycoproteins by hydrazinolysisAnthony H Merry
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
Anal Biochem 304:91-9. 2002.... - Human follicular lymphoma cells contain oligomannose glycans in the antigen-binding site of the B-cell receptorCatherine M Radcliffe
Glycobiology Institute, Medical Research Council Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
J Biol Chem 282:7405-15. 2007..These findings suggest a potential contribution to lymphoma pathogenesis involving antigen-independent interaction of surface immunoglobulin of the B-cell receptor with mannose-binding molecules of innate immunity in the germinal center... - Detailed glycan analysis of serum glycoproteins of patients with congenital disorders of glycosylation indicates the specific defective glycan processing step and provides an insight into pathogenesisMichael Butler
The Glycobiology Institute, Department of Biochemistry, Oxford University, South Parks Road, Oxford, OX1 3QU, UK
Glycobiology 13:601-22. 2003.... - Detailed N-glycan analysis of mannose receptor purified from murine spleen indicates tissue specific sialylationYunpeng Su
Glycobiology Institute, Biochemistry Department, University of Oxford, South Parks Road, OX1, 3QU, United Kingdom
Biochem Biophys Res Commun 384:436-43. 2009..Our study provides a profile of MR N-linked glycosylation that will facilitate our understanding of their physiological role on MR biology in vivo... - Inhibition of mammalian glycan biosynthesis produces non-self antigens for a broadly neutralising, HIV-1 specific antibodyChristopher N Scanlan
Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford
J Mol Biol 372:16-22. 2007..Moreover, this study provides an alternative protein engineering approach to the design of a carbohydrate vaccine for HIV-1 by chemical synthesis... - A strategy to reveal potential glycan markers from serum glycoproteins associated with breast cancer progressionUmi M Abd Hamid
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU, UK
Glycobiology 18:1105-18. 2008..These preliminary findings suggest that specific glycans and glycoforms of proteins may be candidates for improved markers in the monitoring of breast cancer progression... - Controlled glycosylation of therapeutic antibodies in plantsYoram Tekoah
Department of Biochemistry, Glycobiology Institute, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Arch Biochem Biophys 426:266-78. 2004..These results indicate that plant genetic engineering could provide an effective and inexpensive means to control the glycosylation of therapeutic proteins such as mAbs, by the addition of a KDEL signal as a regulatory element... - An analytical and structural database provides a strategy for sequencing O-glycans from microgram quantities of glycoproteinsLouise Royle
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
Anal Biochem 304:70-90. 2002..This approach provides a basis for the analysis of subpicomole quantities of O-glycans from normal levels of natural glycoproteins... - Secretory IgA N- and O-glycans provide a link between the innate and adaptive immune systemsLouise Royle
Glycobiology Institute, Department of Biochemistry, Oxford University, United Kingdom
J Biol Chem 278:20140-53. 2003.... - Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycansD Cameron Dunlop
Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Glycobiology 20:812-23. 2010.... - Identification of N-linked carbohydrates from severe acute respiratory syndrome (SARS) spike glycoproteinGayle Ritchie
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
Virology 399:257-69. 2010..Our data in tissue culture indicate that inhibition of N-glycan processing may be considered as a therapeutic strategy against SARS CoV infections... - Ovarian cancer is associated with changes in glycosylation in both acute-phase proteins and IgGRadka Saldova
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, UK
Glycobiology 17:1344-56. 2007.... - HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database softwareLouise Royle
Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
Anal Biochem 376:1-12. 2008..This technology is also suitable for preparing released glycans for other analytical techniques. Here we demonstrate its application to rheumatoid arthritis using 5 microl of patient serum... - Structural and quantitative analysis of N-linked glycans by matrix-assisted laser desorption ionization and negative ion nanospray mass spectrometryDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
Anal Biochem 376:44-60. 2008..Quantities of glycans were measured by ESI and by matrix-assisted laser desorption ionization mass spectrometry; each technique produced virtually identical results for the neutral desialylated glycans... - Proteomic analysis of glycosylation: structural determination of N- and O-linked glycans by mass spectrometryDavid J Harvey
Department of Biochemistry, Glycobiology Institute, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
Expert Rev Proteomics 2:87-101. 2005..This technique combines high throughput with ease of structural interpretation and provides structural details that are difficult to obtain by classical methods... - Fragmentation of negative ions from carbohydrates: part 2. Fragmentation of high-mannose N-linked glycansDavid J Harvey
Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford, United Kingdom
J Am Soc Mass Spectrom 16:631-46. 2005..C-ions defined the sequence of the constituent monosaccharide residues. Detailed fragmentation mechanisms are proposed to account for many of the diagnostic ions... - Determining the structure of glycan moieties by mass spectrometryDavid J Harvey
University of Oxford, Oxford, United Kingdom
Curr Protoc Protein Sci . 2006..Practical details are given for the purification of samples prior to mass spectrometric analysis and for recording FAB, MALDI, and ESI spectra... - Comparison of the N-linked glycans from soluble and GPI-anchored CD59 expressed in CHO cellsSusan F Wheeler
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford, OX1 3QU, United Kingdom
Glycobiology 12:261-71. 2002..The results show that the presence of the anchor increases the extent of glycan processing, possibly as the result of longer exposure to the glycosyltransferases or to a closer proximity of the protein to these enzymes... - Ionization and fragmentation of N-linked glycans as silver adducts by electrospray mass spectrometryDavid J Harvey
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Rapid Commun Mass Spectrom 19:484-92. 2005..Silver was very efficient at cleaving the glycosidic bonds, so much so that spectra tended to contain glycosidic ions at most possible combinations of the constituent monosaccharides... - "Internal residue loss": rearrangements occurring during the fragmentation of carbohydrates derivatized at the reducing terminusDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, UK
Anal Chem 74:734-40. 2002..A mechanism, involving initial protonation of the amine nitrogen atom of the derivative, is proposed to account for the formation of the observed ions... - Fragmentation of negative ions from carbohydrates: part 3. Fragmentation of hybrid and complex N-linked glycansDavid J Harvey
Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford, United Kingdom
J Am Soc Mass Spectrom 16:647-59. 2005..C-ions defined the sequence of the constituent monosaccharide residues. Detailed fragmentation mechanisms are proposed to account for several of the diagnostic ions... - Collision-induced fragmentation of negative ions from N-linked glycans derivatized with 2-aminobenzoic acidDavid J Harvey
Glycobiology Institute, Department of Biochemistry, University of Oxford, UK
J Mass Spectrom 40:642-53. 2005..Detection limits were around 200 fmol (3 : 1 signal-to-noise ratio)... - Identification of specific glycoforms of major histocompatibility complex class I heavy chains suggests that class I peptide loading is an adaptation of the quality control pathway involving calreticulin and ERp57Catherine M Radcliffe
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
J Biol Chem 277:46415-23. 2002..This is consistent with the hypothesis that the class I loading process is an adaptation of the quality control mechanism involving calreticulin and ERp57... - The glycosylation of human serum IgD and IgE and the accessibility of identified oligomannose structures for interaction with mannan-binding lectinJames N Arnold
Medical Research Council MRC Immunochemistry Unit and Oxford Glycobiology Institute, University of Oxford, United Kingdom
J Immunol 173:6831-40. 2004..This is the first study of the glycosylation of human serum IgD and IgE from nonmyeloma proteins... - Fragmentation of N-linked glycans with a matrix-assisted laser desorption/ionization ion trap time-of-flight mass spectrometerDavid J Harvey
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
Rapid Commun Mass Spectrom 18:2997-3007. 2004..The proposed structures of several cross-ring cleavage ions were confirmed and it was shown that MS3 spectra could be obtained from as little as 10 fmol of glycan... - Fragmentation of negative ions from carbohydrates: part 1. Use of nitrate and other anionic adducts for the production of negative ion electrospray spectra from N-linked carbohydratesDavid J Harvey
Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford, United Kingdom
J Am Soc Mass Spectrom 16:622-30. 2005..Acidic glycans fragmented differently on account of charge localization on the acid functions rather than the hydroxy groups... - Derivatization of sialic acids for stabilization in matrix-assisted laser desorption/ionization mass spectrometry and concomitant differentiation of alpha(2 --> 3)- and alpha(2 --> 6)-isomersSusan F Wheeler
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
Rapid Commun Mass Spectrom 23:303-12. 2009..The method was applied to N-linked glycans released from bovine fetuin and porcine thyroglobulin... - Halogeno-substituted 2-aminobenzoic acid derivatives for negative ion fragmentation studies of N-linked carbohydratesDavid J Harvey
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Rapid Commun Mass Spectrom 19:397-400. 2005.... - Cellular effects of deoxynojirimycin analogues: uptake, retention and inhibition of glycosphingolipid biosynthesisHoward R Mellor
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Biochem J 381:861-6. 2004.... - Enzymatic preparation and isolation of glycopeptidesDavid J Harvey
Oxford Glycobiology Institute, Oxford University, UK
Methods Mol Biol 211:83-9. 2003 - Identification of sites of glycosylationDavid J Harvey
Oxford Glycobiology Institute, Oxford University, UK
Methods Mol Biol 211:371-83. 2003 - Structural determination of N-linked glycans by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometryDavid J Harvey
Glycobiology Institute, Department of Biochemistry, University of Oxford, UK
Proteomics 5:1774-86. 2005..Fragmentation of negative ions followed specific pathways that defined structural details of the glycans that were difficult to obtain by classical methods such as exoglycosidase digestion... - Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update covering the period 1999-2000David J Harvey
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford OX1 3QU, United Kingdom
Mass Spectrom Rev 25:595-662. 2006..Applications of MALDI mass spectrometry to the study of enzymes acting on carbohydrates (glycosyltransferases and glycosidases) and to the synthesis of carbohydrates, are also covered... - A new charge-associated mechanism to account for the production of fragment ions in the high-energy CID spectra of fatty acidsDavid J Harvey
Department of Biochemistry, Oxford Glycobiology Institute, Oxford, United Kingdom
J Am Soc Mass Spectrom 16:280-90. 2005.... - Structural analysis of alpha-Gal and new non-Gal carbohydrate epitopes from specific pathogen-free miniature pig kidneyYun Gon Kim
Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Korea
Proteomics 8:2596-610. 2008..High-mannose type glycans were also abundant (35-43%) but appeared to be ionized more efficiently than the complex glycans by ESI than by MALDI... - Reversed-phase ion-pairing liquid chromatography/ion trap mass spectrometry for the analysis of negatively charged, derivatized glycansLynn A Gennaro
Department of Chemistry and Barnett Institute, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA
Rapid Commun Mass Spectrom 17:1528-34. 2003..With the use of mass spectrometric detection for glycan identity, this allowed relative quantitation of individual components... - Structural analysis of oligosaccharides by atmospheric pressure matrix-assisted laser desorption/ionisation quadrupole ion trap mass spectrometryColin S Creaser
Department of Chemistry and Physics, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK
Rapid Commun Mass Spectrom 16:176-84. 2002..The use of MSn (n = 3, 4) on these complex molecules enabled structural information to be elucidated that confirms data observed in the MS/MS spectra... - Structural characterization of the N-glycan moiety and site of glycosylation in vitellogenin from the decapod crustacean Cherax quadricarinatusIsam Khalaila
Institute for Medical Physics and Biophysics University of Münster, Robert Koch Str 31 D 48149, Munster, Germany
Glycobiology 14:767-74. 2004..Three of the consensus sites for N-glycosylation-namely, Asn(152), Asn(160) and Asn(2493)-were glycosylated with the high-mannose glycans, Man(5-9)GlcNAc(2), and the glucose-capped oligosaccharide Glc(1)Man(9)GlcNAc(2)... - Glycosylation of human pancreatic ribonuclease: differences between normal and tumor statesRosa Peracaula
Departament de Biologia, Universitat de Girona, Campus de Montilivi s n 17071, Girona, Spain
Glycobiology 13:227-44. 2003.... - Altered glycosylation pattern allows the distinction between prostate-specific antigen (PSA) from normal and tumor originsRosa Peracaula
Unitat de Bioquímica i Biologia Molecular, Departament de Biologia, Universitat de Girona, Campus de Montilivi s n 17071, Girona, Spain
Glycobiology 13:457-70. 2003..These carbohydrate differences allow a distinction to be made between PSA from normal and tumor origins and suggest a valuable biochemical tool for diagnosis and follow-up purposes... - Glycoprotein structural genomics: solving the glycosylation problemVeronica T Chang
Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
Structure 15:267-73. 2007..Since the scalability of transient mammalian expression is now comparable to that of bacterial systems, this approach should relieve one of the major bottlenecks in structural genomic analysis... - Sialylation of urinary prothrombin fragment 1 is implicated as a contributory factor in the risk of calcium oxalate kidney stone formationDawn Webber
Department of Chemistry, University of Cape Town, South Africa
FEBS J 273:3024-37. 2006.... - Oligosaccharides modulate the apoptotic activity of glycodelinRajesh Jayachandran
Department of Biochemistry, Indian Institute of Science, Balgalore 560012, India
Glycobiology 16:1052-63. 2006.... - Glycan analysis of monoclonal antibodies secreted in deposition disorders indicates that subsets of plasma cells differentially process IgG glycansLone A Omtvedt
Department of Molecular Biosciences, University of Oslo, Postboks 1041 Blindern, 0316 Oslo, Norway
Arthritis Rheum 54:3433-40. 2006.... - Glycosylation of serum ribonuclease 1 indicates a major endothelial origin and reveals an increase in core fucosylation in pancreatic cancerSílvia Barrabés
Unitat de Bioquímica i Biologia Molecular, Departament de Biologia, Universitat de Girona, Campus de Montilivi, Girona 17071, Spain
Glycobiology 17:388-400. 2007..RNase 1 from EA.hy926 was then purified and presented a glycosylation profile very similar to that from serum RNase 1, suggesting that endothelial cells are the main source of this enzyme... - Capillary electrophoresis/electrospray ion trap mass spectrometry for the analysis of negatively charged derivatized and underivatized glycansLynn A Gennaro
Department of Chemistry and Barnett Institute, Northeastern University, Boston, MA 02115, USA
Rapid Commun Mass Spectrom 16:192-200. 2002..This technique presents a complementary method to existing methodologies for the profiling of glycan mixtures... - Contrasting glycosylation profiles between Fab and Fc of a human IgG protein studied by electrospray ionization mass spectrometryYusuke Mimura
Division of Immunity and Infection, Medical School, Edgbaston, Birmingham, B15 2TT, UK
J Immunol Methods 326:116-26. 2007..Thus, combination of the two endoglycosidases can provide a simple means of glycan analysis of both Fab and Fc by ESI-MS, which may contribute to the development of therapeutic IgG with customized glycan profiles... - Changes of serum glycans during sepsis and acute pancreatitisOlga Gornik
Department of Biochemistry and Molecular Biology, University of Zagreb Faculty of Pharmacy and Biochemistry, 10000, Zagreb, Croatia
Glycobiology 17:1321-32. 2007.... - Function and glycosylation of plant-derived antiviral monoclonal antibodyKisung Ko
Biotechnology Foundation Laboratories at Thomas Jefferson University, 1020 Locust Street, JAH, Philadelphia, PA 19107, USA
Proc Natl Acad Sci U S A 100:8013-8. 2003..The mAbP was as efficient as HRIG for post-exposure prophylaxis against rabies virus in hamsters, indicating that differences in N-glycosylation do not affect the efficacy of the antibody in this model... - The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.)Bergljot Magnadottir
Institute for Experimental Pathology, University of Iceland, Reykjavik, Iceland
Fish Shellfish Immunol 12:209-27. 2002..The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody... - Localization and characterization of polysialic acid-containing N-linked glycans from bovine NCAMMaren von der Ohe
, University of Hamburg, D-20246 Hamburg, Germany
Glycobiology 12:47-63. 2002..In conclusion, we observed a structural variability and high regional selectivity for the PSA-glycans attached to the NCAM molecule that are most likely influencing its biological functions... - Monitoring endoplasmic reticulum-to-Golgi traffic of a plant calreticulin by protein glycosylation analysisLorella Navazio
Dipartimento di Biologia, Universita di Padova, Via U Bassi 58 B, 35131 Padova, Italy
Biochemistry 41:14141-9. 2002..The final fate of the protein after its complete maturation is still obscure... - Differential effects of alpha subunit Asparagine56 oligosaccharide structure on equine lutropin and follitropin hybrid conformation and receptor-binding activityGeorge R Bousfield
Department of Biological Sciences, Wichita State University, Wichita, Kansas 67260, USA
Biochemistry 43:10817-33. 2004..Oligosaccharide removal altered the conformation of most hybrids, suggesting that alphaAsn(82) oligosaccharide (located in alphaL3) also influenced gonadotropin conformation... - Differentiation between sulphated and phosphated carbohydrates in low-resolution matrix-assisted laser desorption/ionization mass spectraDavid J Harvey
Rapid Commun Mass Spectrom 19:287-8. 2005 - All-or-none N-glycosylation in primate follicle-stimulating hormone beta-subunitsGeorge R Bousfield
Wichita State University, Wichita, KS, United States
Mol Cell Endocrinol 260:40-8. 2007..Two primate species exhibited the same all-or-none pattern of pituitary FSHbeta glycosylation... - Plant N-glycan profiling of minute amounts of materialMartial Seveno
CNRS FRE 3090, IFRMP 23, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France Medicago Inc, Quebec, QC G1V 3V9, Canada
Anal Biochem 379:66-72. 2008.... - The identification and characterization of xenoantigenic nonhuman carbohydrate sequences in membrane proteins from porcine kidneyYun-Gon Kim
Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Korea
Proteomics 6:1133-42. 2006..This total glycan profile of the membrane proteins from porcine kidney will thus provide important information for the study of molecular interactions between antigenic carbohydrates and proteins in xenotransplantation... - Remarkable selective glycosylation of the immunoglobulin variable region in follicular lymphomaKaty J McCann
Genetic Vaccine Group, Cancer Sciences Division, University of Southampton School of Medicine, Southampton, UK
Mol Immunol 45:1567-72. 2008..The remarkable selective glycosylation within the heavy chain V region gene of FL apparently permits only limited processing to oligomannose at somatically mutated motifs, creating a feature exploitable by GC lymphomas...