Research Topics
| Jane GreatorexSummaryAffiliation: University of Cambridge Country: UK Publications
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Detail Information
Publications
The retroviral RNA dimer linkage: different structures may reflect different rolesJane Greatorex
Division of Infectious Diseases, Dept of Medicine, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
Retrovirology 1:22. 2004..Differences and similarities in both secondary and tertiary structure are discussed. The implication of varying roles for the dimer linkage in related viruses is considered...
Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging, normal particle maturation and viral infectivityAnne L'Hernault
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge CB2 2QQ, UK
Retrovirology 4:90. 2007..In this study, we analysed the role of the HIV-2 packaging signal in genomic RNA dimerisation in vivo and its implication in viral replication...
Mutation of the Rev-binding loop in the human immunodeficiency virus 1 leader causes a replication defect characterized by altered RNA trafficking and packagingJane S Greatorex
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge CB2 2QQ, UK
J Gen Virol 87:3039-44. 2006..The loop A sequence, which is 98% conserved amongst viral isolates, is implicated in several cis-acting functions critical to virus viability...
Steric block high affinity oligonucleotide analogues: a new tool for mapping RNA-protein binding sitesDouglas Brown
Department of Medicine, University of Cambridge, Addenbrookes Hospital, Cambridge, United Kingdom
Nucleosides Nucleotides Nucleic Acids 27:196-212. 2008..We also demonstrate direct binding of oligonucleotide to their binding sites and confirm this interaction does not alter global RNA conformation, making them highly specific, nondisruptive probes of RNA protein interactions...
Structure and stability of wild-type and mutant RNA internal loops from the SL-1 domain of the HIV-1 packaging signalJane Greatorex
Department of Medicine, University of Cambridge, Level 5, Addenbrookes Hospital, Hills Road, CB2 2QQ, Cambridge, UK
J Mol Biol 322:543-57. 2002..In a manner analogous to SL-3, we propose that conformational flexibility at this site may facilitate melting of the structure during Gag protein capture or genomic RNA dimerisation...
Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNAJose Gallego
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
J Biol Chem 278:40385-91. 2003..HIV-1 appears to have a second Rev-binding site close to the major splice donor site that may have an additional role in the viral life cycle...
