Research Topics
Species | Felix M MunkongeSummaryAffiliation: Imperial College Country: UK Publications
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Detail Information
Publications
Emerging significance of plasmid DNA nuclear import in gene therapyFelix M Munkonge
Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, UK
Adv Drug Deliv Rev 55:749-60. 2003....
Identification and functional characterization of cytoplasmic determinants of plasmid DNA nuclear importFelix M Munkonge
Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW3 6LR, United Kingdom
J Biol Chem 284:26978-87. 2009..Increasing the potential for exogenously added pDNA to bind intracellular transport cofactors may enhance the potency of non-viral gene transfer...
Limitations of the murine nose in the development of nonviral airway gene transferUta Griesenbach
Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
Am J Respir Cell Mol Biol 43:46-54. 2010..At the current levels of gene transfer efficiency achievable with nonviral vectors, the murine nose is of limited value as a stepping stone to human trials...
Nondisruptive, sequence-specific coupling of fluorochromes to plasmid DNAElizabeth Hillery
Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW3 6LR, UK
Anal Biochem 352:169-75. 2006..Thus, this method allows for the sequence-specific coupling of commercially available fluorochromes to DNA expression vectors while retaining biological function...
The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airwaysUta Griesenbach
Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London SW3 6LR, UK
Biomaterials 31:2665-72. 2010..0001, n=18). This study suggests that contact time of non-viral gene transfer agents is a key factor for gene delivery, and suggests two methods which may be translatable for use in man...
Assessment of CFTR function after gene transfer in vitro and in vivoUta Griesenbach
Department of Gene Therapy, Faculty of Medicine, Imperial College London, UK
Methods Mol Biol 433:229-42. 2008..Here, we describe pre-clinical methods related to assessing correction of the CF chloride transport defect...
The efficacy of a 'master switch gene' HIF-1alpha in a porcine model of chronic myocardial ischaemiaAmanda Heinl-Green
Department of Gene Therapy, Faculty of Medicine, The National Heart and Lung Institute, Imperial College London, Manresa Road, London SW3 6LR, UK
Eur Heart J 26:1327-32. 2005..Therapeutic angiogenesis is a potential new treatment for patients unsuitable for conventional revascularization strategies. We investigated angiogenesis via a 'master switch gene' hypoxia inducible factor (HIF-1alpha)...
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expressionStephen C Hyde
Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
Nat Biotechnol 26:549-51. 2008..Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation...
