Research Topics
| Wengang ChaiSummaryAffiliation: Imperial College Country: UK Publications
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Detail Information
Publications
Relative susceptibilities of the glucosamine-glucuronic acid and N-acetylglucosamine-glucuronic acid linkages to heparin lyase IIIWengang Chai
MRC Glycosciences Laboratory, Imperial College Faculty of Medicine, Northwick Park Hospital Campus, Watford Road, Harrow, Middlesex, UK
Biochemistry 43:8590-9. 2004..It is possible that these are products of an additional glycosidase activity of lyase III, although other mechanisms cannot be completely ruled out...- Structural determination of novel lacto-N-decaose and its monofucosylated analogue from human milk by electrospray tandem mass spectrometry and 1H NMR spectroscopyWengang Chai
MRC Glycosciences Laboratory, Imperial College Faculty of Medicine, Northwick Park and St Mark s Campus, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
Arch Biochem Biophys 434:116-27. 2005.... - Analysis of chain and blood group type and branching pattern of sialylated oligosaccharides by negative ion electrospray tandem mass spectrometryWengang Chai
MRC Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Northwick Park and St Mark s Campus, Harrow, Middlesex, UK
Anal Chem 78:1581-92. 2006..The structure and sequence assignment was corroborated by methylation analysis and 1H NMR spectroscopy... - Neoglycolipid probes prepared via oxime ligation for microarray analysis of oligosaccharide-protein interactionsYan Liu
Glycosciences Laboratory, Imperial College London, Northwick Park and St Mark s Campus, Harrow, Middlesex HA1 3UJ, United Kingdom
Chem Biol 14:847-59. 2007.... - Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose coreHeide Kogelberg
MRC Glycosciences Laboratory, Imperial College Faculty of Medicine, Northwick Park Institute for Medical Research, Harrow, Middlesex, UK
Eur J Biochem 271:1172-86. 2004.... - Neoglycolipid technology: deciphering information content of glycomeWengang Chai
MRC Glycosciences Laboratory, Imperial College London, Northwick Park Hospital Campus, Harrow, Middlesex, HA1 3UJ, United Kingdom
Methods Enzymol 362:160-95. 2003 - Multifaceted approaches including neoglycolipid oligosaccharide microarrays to ligand discovery for malectinAngelina S Palma
Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Northwick Park Hospital Campus, Harrow, Middlesex, United Kingdom
Methods Enzymol 478:265-86. 2010..Thus, the way is open to the identification of the role of malectin in the N-glycosylation pathway... - High and low affinity carbohydrate ligands revealed for murine SIGN-R1 by carbohydrate array and cell binding approaches, and differing specificities for SIGN-R3 and langerinChristine Galustian
Glycosciences Laboratory, Imperial College London, Northwick Park and St Mark s Hospital campus, Watford Road, Harrow, Middlesex HA1 3UJ, UK
Int Immunol 16:853-66. 2004..But there is a close relationship between dextran recognition and mannose/fucose recognition, with dextran- and mannose-conjugates co-localizing in intracellular compartments... - An investigation of the interactions of E-selectin with fuco-oligosaccharides of the blood group familyMaria J Martin
Imperial College School of Medicine, Northwick Park Campus, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
Glycobiology 12:829-35. 2002..These findings contribute to understanding the molecular basis of E-selectin recognition and could influence future designs of selectin antagonists as possible therapeutic substances... - Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharidesAngelina S Palma
Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Northwick Park and St Mark's Campus, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
J Biol Chem 281:5771-9. 2006.... - Preparation of neoglycolipids with ring-closed cores via chemoselective oxime-ligation for microarray analysis of carbohydrate-protein interactionsYan Liu
The Glycosciences Laboratory, Imperial College London, Harrow Middlesex, UK
Methods Enzymol 415:326-40. 2006..Thus, AO-NGLs have broadened the applicability of NGLs as probes in studies of carbohydrate-protein interactions... - The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucinsAngelina S Palma
Glycosciences Laboratory, Department of Medicine, Imperial College London, Northwick Park and St Marks Campus, Watford Road, Harrow, Middlesex, UK
Biochem Biophys Res Commun 408:548-52. 2011.... - N-glycolyl GM1 ganglioside as a receptor for simian virus 40Maria A Campanero Rhodes
Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Northwick Park and St Mark s Campus, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
J Virol 81:12846-58. 2007..Our results open the way not only to detailed studies of SV40 infection in relation to receptor expression in host cells but also to the monitoring of changes that may occur with time in receptor usage by the virus... - Neoglycolipid-based "designer" oligosaccharide microarrays to define ?-glucan ligands for Dectin-1Angelina S Palma
The Glycosciences Laboratory, Department of Medicine, Imperial College London, Hammersmith Campus, London, UK
Methods Mol Biol 808:337-59. 2012.... - Neoglycolipid-based oligosaccharide microarray system: preparation of NGLs and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analysesYan Liu
The Glycosciences Laboratory, Department of Medicine, Imperial College London, Hammersmith Campus, London, UK
Methods Mol Biol 808:117-36. 2012.... - Oligosaccharide microarrays to decipher the glyco codeTen Feizi
The Glycosciences Laboratory, Imperial College London, Northwick Park Hospital Campus, Watford Road, Harrow, Middlesex HA1 3UJ, UK
Nat Rev Mol Cell Biol 5:582-8. 2004 - Altered receptor specificity and cell tropism of D222G hemagglutinin mutants isolated from fatal cases of pandemic A(H1N1) 2009 influenza virusYan Liu
The Glycosciences Laboratory, Department of Medicine, Imperial College London, UK
J Virol 84:12069-74. 2010..These features of 222G mutants may contribute to exacerbation of disease... - Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactionsShigeyuki Fukui
Glycosciences Laboratory, Imperial College Faculty of Medicine, Northwick Park Hospital, Watford Road, Harrow, Middlesex HA1 3UJ, UK
Nat Biotechnol 20:1011-7. 2002..The approach opens the way for discovering new carbohydrate-recognizing proteins in the proteome and for mapping the repertoire of carbohydrate recognition structures in the glycome... - Atomic resolution insight into host cell recognition by Toxoplasma gondiiTharin M A Blumenschein
Division of Molecular Biosciences, Imperial College London, London, UK
EMBO J 26:2808-20. 2007..Our work uncovers new features of parasite-receptor interactions at the early stages of host cell invasion, which will assist the design of new therapeutic strategies... - The role of sialyl glycan recognition in host tissue tropism of the avian parasite Eimeria tenellaLivia Lai
Division of Molecular Biosciences, Imperial College London, South Kensington Campus, London, United Kingdom
PLoS Pathog 7:e1002296. 2011..Finally, a preliminary chicken immunization trial provides evidence that recombinant EtMIC3 protein and EtMIC3 DNA are effective vaccine candidates... - Carbohydrate microarrays - a new set of technologies at the frontiers of glycomicsTen Feizi
The MRC Glycosciences Laboratory, Imperial College London, Northwick Park and St Mark s Hospital campus, Watford Road, Harrow, Middlesex HA1 3UJ, UK
Curr Opin Struct Biol 13:637-45. 2003..New leads to biological pathways that involve carbohydrate-protein interactions and new therapeutic targets are among biomedically important outcomes anticipated from applications of carbohydrate microarrays... - Branching pattern and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometryWengang Chai
MRC Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Harrow, Middlesex, United Kingdom
J Am Soc Mass Spectrom 13:670-9. 2002.... - The structural motif in chondroitin sulfate for adhesion of Plasmodium falciparum-infected erythrocytes comprises disaccharide units of 4-O-sulfated and non-sulfated N-acetylgalactosamine linked to glucuronic acidWengang Chai
Medical Research Council Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Harrow, Middlesex HA1 3UJ, United Kingdom
J Biol Chem 277:22438-46. 2002.... - On-line overpressure thin-layer chromatographic separation and electrospray mass spectrometric detection of glycolipidsWengang Chai
MRC Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Watford Road, Harrow, Middlesex HA 1 3UJ, UK
Anal Chem 75:118-25. 2003..Aspects for further improvement of OPLC instrumentation include use of narrower TLC plate dimensions and refined design of the eluate exit system... - Binding of sucrose octasulphate to the C-type lectin-like domain of the recombinant natural killer cell receptor NKR-P1A observed by NMR spectroscopyHeide Kogelberg
The Glycosciences Laboratory Faculty of Medicine Imperial College of Science Technology and Medicine Northwick Park Institute of Medical Research Harrow, Middlesex, HA1 3UJ, UK
Chembiochem 3:1072-7. 2002..1)H titration studies indicate that the binding is specific. These findings raise the possibility that NKR-P1A recognises sulphated natural ligands in common with certain other members of the C-type lectin family... - Sequence analysis of alginate-derived oligosaccharides by negative-ion electrospray tandem mass spectrometryZhenqing Zhang
Institute of Marine Drug and Food, Ocean University of China, Shandong, China
J Am Soc Mass Spectrom 17:621-30. 2006.... - Identification of a low affinity mannose 6-phosphate-binding site in domain 5 of the cation-independent mannose 6-phosphate receptorSreelatha T Reddy
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 279:38658-67. 2004..Taken together, these results demonstrate that the CI-MPR contains a third Man-6-P recognition site that is located in domain 5 and that exhibits lower affinity than the carbohydrate-binding sites present in domains 1-3 and 9... - First synthesis of heparan sulfate tetrasaccharides containing both N-acetylated and N-unsubstituted glucosamine-search for putative 10E4 epitopesDaniel Hamza
Laboratoire de Chimie Organique Multifonctionnelle, , , UMR CNRS-UPS 8182, , , 91405 Orsay Cedex, France
Chembiochem 7:1856-8. 2006 - Structural basis for binding of Plasmodium falciparum erythrocyte membrane protein 1 to chondroitin sulfate and placental tissue and the influence of protein polymorphisms on binding specificityJames G Beeson
The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
J Biol Chem 282:22426-36. 2007..These findings have significant implications for understanding the pathogenesis and biology of malaria, particularly during pregnancy, and the development of targeted interventions... - Sequence determination of sulfated carrageenan-derived oligosaccharides by high-sensitivity negative-ion electrospray tandem mass spectrometryGuangli Yu
Institute of Marine Drug and Food, Ocean University of China, Qingdao, Shandong 266003, China
Anal Chem 78:8499-505. 2006..Due to the anionic nature of the sulfate present, high-sensitivity detection (1-5 pmol, using a hexasaccharide as an example) was obtained...