M J Doughty

..To use transmission electron microscopy (TEM) to investigate further the ultrastructural details of the collagen fibrils linking the anterior limiting lamina (ALL; Bowman's membrane) of the human cornea to the anterior stromal lamellae...

..To investigate collagen fibrils of the equatorial sclera in relation to the age-related changes in eye size in sheep...

..The aim of this study was to compare two methods of assessments of the coefficient of variation (COV) of endothelial cell area...

..Little has been published on the reliability of estimates of the coefficient of variation (COV) in cell area for human corneal endothelia. The present study compares two methods...

..To assess the results from using a commercially available phenol red thread (PRT) test with the eyes being kept open (as recommended by the company) vs a closed eye protocol (as used by some investigators)...

..To report a case of conjunctival squamous metaplasia associated with oral carbamazepine use...

..To assess whether the difference between midperipheral (paracentral) cell density (MPCD) and central cell density (CCD) values of the corneal endothelium could be related to the duration of soft contact lens wear...

..To assess whether the use of Orbscan pachymetry, as an alternative to ultrasound pachymetry, had an effect on the IOP-CCT relationship for Goldmann tonometry in normal human eyes...

..The current study was therefore undertaken to assess the corneal thickness profile along the horizontal meridian by this scanning slit light method, comparing the data with that obtained with an ultrasound pachymeter...

..To assess the time-dependent changes in ocular comfort following unilateral instillation of preservative-free rose bengal 1% eyedrops when compared with saline 0.9% or proxymetacaine 0.5%...

..To develop a method for the quantitative analyses of the ring-shaped features usually observed on the surface of mammalian corneal epithelial cells...

..To further analyze the possible causes of variability in spontaneous eye blink activity in apparently healthy humans...

..It may also be used for the management of corneal and conjunctival abrasions and foreign body injuries, or some cases of chronic blepharitis...

..To illustrate a method of quantitative scanning electron microscopy (SEM) for the evaluation of the corneal endothelia of small animals by comparing two commonly used fixation methods...

..An acoustic correction factor (AF) was introduced later to compensate for this difference. The goal of this review was to assess the magnitude and consistency of the difference, as well as to assess how useful the AF had been...

..To compare the outcome measures from two different methods for estimating the coefficient of variation (COV) in cell area for human corneal endothelia...

..To quantitatively assess the effects of pH on the corneal stroma under an applied hydrostatic pressure...

..To specifically assess the nucleus size and its relationship to cell size for human bulbar conjunctival cells...

..The purpose of this work is to assess the reliability of endothelial cell-density (ECD) estimates in corneas with different severity pseudoguttata...

..To review the reported effects of contact lens wear on the surface epithelial cells of the human conjunctiva as assessed by conjunctival impression cytology (CIC)...

..The aim of the present study was to assess central corneal thickness (CCT) and intraocular pressure (IOP) in eyes where the corneas were affected by different degrees of severity of endothelial pseudo-guttata or guttata...

..To review the reported effects of contact lens wear on the goblet cells of the human conjunctiva...

..To objectively assess the cell and nucleus dimensions of human bulbar conjunctival cells in female soft contact lens wearers to illustrate a method for assessment of the nucleus-to-cytoplasm ratio based on simple linear measures...

..5)...

..To re-assess and further quantify the pH-related changes in the absorbance and fluorescence emission spectra of sodium fluorescein in buffered aqueous solutions...

..To assess the level of agreement for assignment of a normal grade for human ocular surface bulbar conjunctival cells as collected by conjunctival impression cytology...

..To objectively review the outcome of clinical studies where rose bengal stain (RB) has been used as an outcome measure to assess the efficacy of artificial tears (AT) in patients with dry eye...

..A number of recent studies have reported high spontaneous eyeblink rate (SEBR) values in apparently normal subjects, but the reasons for this are unclear...

..To investigate, by audit, the intraocular pressure (IOP) and central corneal thickness (CCT) of patients attending a routine glaucoma clinic, in order to assess the potential effect of CCT on IOP measures...

..To quantitatively assess surface features of corneal epithelial cells with particular emphasis on regional differences in cell size or shape...

..To assess whether fluorexon strips could be used in tear stability [tear film break-up time (TBUT)] measures, as compared with fluorescein strips...

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..To qualitatively and quantitatively assess the surface cells of the bulbar conjunctiva of slaughterhouse-obtained bovine eyes by impression cytology (IC) and scanning electron microscopy (SEM)...

..A study was thus undertaken on a standardised preparation to assess the relative magnitude of this swelling and its relationship to thickness of the preparations...

..In all solutions, protein and proteoglycans were detected, but these ranged from around 1 to 10% of the samples with the bicarbonate-buffered solutions, to around 30% with the use of some phosphate buffers or saline...

..To examine the surface of pre-corneal mucous layer by scanning electron microscopy (SEM) with the view to assessing whether it was an amorphous, fibrous or porous structure...

..To further assess the spontaneous eyeblink rate (SEBR) in primary gaze in young adults to further define the causes of variance in this activity...

..An adequate volume of tears is considered essential to prevent desiccation of the exposed ocular surface, especially in elderly individuals. Few assessments of tear meniscus height (TMH) have however been reported for elderly individuals...

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..The reason for this analysis was to try to identify why such different values for the spontaneous eyeblink rate (SEBR) have been reported...

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..To qualitatively assess the frequency and intensity of the staining of Marx's line with lissamine green dye...

..To assess whether the size of the cells obtained by conjunctival impression cytology can be quantitatively assessed by measurement of the longest dimension of the cells...

..To qualitatively and quantitatively assess the size and shape of the surface cells of the bovine corneal epithelium as assessed by scanning electron microscopy...

..The rabbit palpebral conjunctival surface is thus distinctly different from the peripheral corneal epithelium across which it slides following eyelid closure...

..Human occupational exposure to ultraviolet radiation (UVR) in welding is considered a risk for endothelial damage but the evidence for such an effect is limited...

..These types of interrelationships could also apply to other endothelia...

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..In mixed salts solution, 100% inhibition of swelling could be achieved at 2% CPC and BAC. In pure water, the relative swelling was much more substantial and could only be attenuated by CPC...

..J. Physiol. (Lond.) 298 (1980) 453-470). It is concluded that pH changes in the physiological range can have a small but reproducible impact on the swelling kinetics of the isolated mammalian corneal stroma ex vivo...

..We sought to determine the variance in endothelial cell density (ECD) estimates for human corneal endothelia...

..The first four methods however yielded cells with unacceptable artefacts (especially distortion). More details should be provided of the primary fixation method used...

..Occupational exposure to a mixture of IRR, perhaps some UVR as well as thermal convection effects, can apparently result in morphological changes in the human corneal endothelium. These may be the result of IRR-stimulated cell division...

..The overall cell shrinkage and relative accentuation of the cell-cell borders was confirmed by SEM, which also revealed that the 11 to 20% area shrinkage was also related to the number of cell sides...

..To assess the repeatability of Orbscan measures on right versus left eyes...

..To investigate age-related differences in central and peripheral corneal thickness in humans with univariate and multivariate regression analyses...

..To compare corneal thickness measurements obtained by a slit-scanning method with those obtained by noncontact specular microscopy, with a focus on the midperipheral (3.0 mm) region of the cornea...

..This study outcome can be taken to indicate that despite the occurrence of welders flash episodes, the eye protection habitually used by these workers was adequate to protect their corneal endothelium...

..The purpose of this study is to assess whether improved oxygen availability to the cornea resulted in changes in the corneal endothelium...

..The heterogeneous nature of this sclero-corneal interface requires further investigation to define the mechanism of the effect...

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..These aspects of the morphometry of small collagen fibrils therefore need to be carefully specified...

..5 mm from centre) and peripheral (4.5 mm from centre) region of the cornea...

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..To compare repeatability of the measures of corneal thickness obtained by slit-scanning light method (Orbscan II) with those obtained by an ultrasound pachymeter, with special interest in the peripheral region of the cornea...

..The removal of exogenous stimuli by anaesthesia does not shift the eyeblink pattern to a single type, so indicates endogenous control...

..CONCLUSIONS: The results indicate that chronic alcohol use can adversely affect subjective static accommodation, especially in younger alcoholics, as well as cause slight mydriasis...

..The purpose of this study was to assess the endothelium of corneal grafts by in vivo confocal microscopy (IVCM), and to evaluate an automated endothelial software system in comparison with a manual cell count and planimetry...

..These types of effects need to be further assessed in older patients with ocular hypertension, or other glaucoma suspects...

..An exploratory study was undertaken to assess three measures and their relationship in non-contact lens wearers and in contact lens wearers...

..To determine the total number of lamellae within the central region of the human corneal stroma by using a continuous section through the corneal stroma and at the ultrastructural resolution of the electron microscope...

..To compare, in routine optometric practice, the outcome of keeping successful soft contact lens (SCL) wearers in their lenses to re-fitting them with continuous wear of silicone hydrogel (SiH) lenses...

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..CONCLUSIONS: Intra- and inter-sample variability in collagen FDs is very similar, but the analysis indicates that the collagen fibrils are not homogeneous and that closely adjacent lamellae can have subtle differences in average FD...