Mesoangioblast stem cells ameliorate muscle function in dystrophic dogsMaurilio Sampaolesi
San Raffaele Scientific Institute, , Stem Cell Research Institute, Via Olgettina 58, 20132 Milan, Italy
Nature 444:574-9. 2006
..The outcome is a remarkable clinical amelioration and preservation of active motility. These data qualify mesoangioblasts as candidates for future stem cell therapy for Duchenne patients...
- Identification of a putative pathway for the muscle homing of stem cells in a muscular dystrophy model
Yvan Torrente
Department of Neurological Sciences, Stem Cell Laboratory, University of Milan, Padiglione Ponti, Ospedale Policlinico, Via Francesco Sforza 35, 20122 Milan, Italy
J Cell Biol 162:511-20. 2003
..This discovery will aid in the improvement of a potential therapy for muscular dystrophy based on the systemic delivery of MDSCs...
- High-resolution X-ray microtomography for three-dimensional visualization of human stem cell muscle homing
Y Torrente
Department of Neurological Sciences, Centro Dino Ferrari, University of Milan, Padiglione Ponti, Ospedale Policinico, Via Francesco Sforza 35, 20122 Milan, Italy
FEBS Lett 580:5759-64. 2006
..We show that X-ray computed microtomography offers the possibility to detect with high definition and resolution human cells after transplantation, and opens new possibilities for both experimental stem cell research...
- Human circulating AC133(+) stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle
Yvan Torrente
Stem Cell Laboratory, Department of Neurological Science, Instituto di Ricovero e Cura a Carattere Scientifico Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Italy
J Clin Invest 114:182-95. 2004
..As these cells can be isolated from the blood, manipulated in vitro, and delivered through the circulation, they represent a possible tool for future cell therapy applications in DMD disease or other muscular dystrophies...
- Autologous transplantation of muscle-derived CD133+ stem cells in Duchenne muscle patients
Y Torrente
Fondazione IRCCS Ospedale Maggiore Policlinico of Milan, Department of Neurological Sciences, Dino Ferrari Center, University of Milan, Italy
Cell Transplant 16:563-77. 2007
..No local or systemic side effects were observed in all treated DMD patients. Treated patients had an increased ratio of capillary per muscle fibers with a switch from slow to fast myosin-positive myofibers...
- Intraarterial injection of muscle-derived CD34(+)Sca-1(+) stem cells restores dystrophin in mdx mice
Y Torrente
Instituto de Ricovero e Cura a Carattere Scientifico (IRCCS) Ospedale Maggiore Policlinico, 20122 Milan, Italy
J Cell Biol 152:335-48. 2001
..Our results showed that the muscle-derived stem cells first attach to the capillaries of the muscles and then participate in regeneration after muscle damage...
- CD20-related signaling pathway is differently activated in normal and dystrophic circulating CD133(+) stem cells
D Parolini
Department of Neurological Sciences, Fondazione IRCCS Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Italy
Cell Mol Life Sci 66:697-710. 2009
..Presented findings represent the starting point toward the expansion of knowledge on pathways involved in the pathology of DMD and in the behavior of dystrophic blood-derived CD133(+) stem cells...
- In vitro and in vivo tetracycline-controlled myogenic conversion of NIH-3T3 cells: evidence of programmed cell death after muscle cell transplantation
R Del Bo
Dino Ferrari Center, Institute of Clinical Neurology, University of Milan, Italy
Cell Transplant 10:209-21. 2001
..This work opens the question whether apoptotic phenomena also play a general role as limiting factors of cell-mediated gene therapy of inherited muscle disorders...
- T and B lymphocyte depletion has a marked effect on the fibrosis of dystrophic skeletal muscles in the scid/mdx mouse
A Farini
Fondazione IRCCS Ospedale Maggiore Policlinico of Milan, Department of Neurological Sciences, Dino Ferrari Center, University of Milan, Italy
J Pathol 213:229-38. 2007
..These data demonstrate a correlation between the absence of B and T lymphocytes and loss of fibrosis accompanied by reduction of TGF-beta1, suggesting the importance of modulation of the immune system in DMD...
- T-antigen regulated expression reduces apoptosis of tag-transformed human myoblasts
S Corti
Institute of Clinical Neurology, IRCCS Ospedale Maggiore Policlinico, University of Milan, Italy
Cell Mol Life Sci 58:135-40. 2001
..The observation that apoptosis can be prevented by the down-regulation of Tag suggests that the programmed cell death induced in transformed cells can be reversed, and confirms the regulatory efficiency of the human vimentin promoter...
- Tumor necrosis factor-alpha (TNF-alpha) stimulates chemotactic response in mouse myogenic cells
Y Torrente
Centro Dino Ferrari, Institute of Clinical Neurology, University of Milan, Milan, Italy
Cell Transplant 12:91-100. 2003
..We propose that TNF-alpha may promote myoblast migration directly through chemotactic activity and indirectly by enhancing MMP activity at the site of muscle injury...
- Fate of autologous dermal stem cells transplanted into the spinal cord after traumatic injury (TSCI)
A Gorio
Laboratory of Pharmacology, Department of Medicine, Surgery and Dentistry, Faculty of Medicine, University of Milan, Via A Di Rudini 8, Milano 20142, Italy
Neuroscience 125:179-89. 2004
..On the other hand there is the possibility of dye transfer from labeled cells to endogenous cells, and this might influence the data...
- Restoration of human dystrophin following transplantation of exon-skipping-engineered DMD patient stem cells into dystrophic mice
Rachid Benchaouir
Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Via F Sforza 35, 20122 Milan, Italy
Cell Stem Cell 1:646-57. 2007
..These data demonstrate that autologous engrafting of blood or muscle-derived CD133+ cells, previously genetically modified to reexpress a functional dystrophin, represents a promising approach for DMD...
- VCAM-1 expression on dystrophic muscle vessels has a critical role in the recruitment of human blood-derived CD133+ stem cells after intra-arterial transplantation
Manuela Gavina
Stem Cell Laboratory, Department of Neurological Sciences, IRCCS, Centro Dino Ferrari, University of Milan, Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, Italy
Blood 108:2857-66. 2006
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- Skin-derived stem cells transplanted into resorbable guides provide functional nerve regeneration after sciatic nerve resection
C Marchesi
Fondazione IRCCS Ospedale Maggiore Policlinico Mangiagalli e Regina Elena of Milan, Stem Cell Laboratory, Department of Neurological Sciences, Centro Dino Ferrari, University of Milan, Italy
Glia 55:425-38. 2007
..These data support the hypothesis that SDSCs could represent a tool for future cell therapy applications in peripheral nerve regeneration...
- Extracorporeal circulation as a new experimental pathway for myoblast implantation in mdx mice
Y Torrente
Centro Dino Ferrari, Institute of Clinical Neurology, University of Milan, Italy
Cell Transplant 8:247-58. 1999
..2% of the total muscle fibers. These results demonstrate that the extracorporeal circulation allows selective myoblast-mediated gene transfer to muscles, opening new perspectives in muscular dystrophy gene therapy...
- Cell based therapy for Duchenne muscular dystrophy
Andrea Farini
Stem Cell Laboratory, Department of Neurological Science, Centro Dino Ferrari, University of Milan, Fondazione IRCCS Policlinico Mangiagalli Regina Elena, Italy
J Cell Physiol 221:526-34. 2009
..We speculated that the most promising possibility for the treatment of DMD is a combination of different approaches, such as gene and stem cell therapy...
- The new challenge of stem cell: brain tumour therapy
F Colleoni
Stem Cell Laboratory, Department of Neurological Science, University of Milan, Fondazione IRCCS Ospedale Maggiore Policlinico, Centro Dino Ferrari, Italy
Cancer Lett 272:1-11. 2008
..In this review we highlight the close relationship between normal neural stem cells and brain tumour stem cells and also suggest the possible clinical implications that these similarities could offer...
- Human skin-derived stem cells migrate throughout forebrain and differentiate into astrocytes after injection into adult mouse brain
Marzia Belicchi
Department of Neurological Sciences, Stem Cell Laboratory, Centro Dino Ferrari, IRCCS Ospedale Maggiore Policlinico, University of Milan, Milan, Italy
J Neurosci Res 77:475-86. 2004
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- Correlation of circulating CD133+ progenitor subclasses with a mild phenotype in Duchenne muscular dystrophy patients
Chiara Marchesi
Stem Cell Laboratory, Department of Neurological Science, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Milan, Italy
PLoS ONE 3:e2218. 2008
..Here we assessed whether the levels of certain stem cells may predict the progression of Duchenne muscular dystrophy (DMD)...
- [Restoration of human dystrophin following transplantation of exon-skipping-engineered DMD patient stem cells into dystrophic mice]
Rachid Benchaouir
Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Via F. Sforza 35, Milan, Italy
Med Sci (Paris) 24:99-101. 2008
- Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2
Marzia Belicchi
Stem Cell Laboratory, Department of Neurological Sciences, Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Universita di Milano, Padiglione Ponti, Via F Sforza 35, 20122 Milano, Italy
Biomaterials 31:5385-96. 2010
..The exploitation of cluster-assembled ns-TiO(2) substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical...
- Expression of parathyroid-specific genes in vascular endothelial progenitors of normal and tumoral parathyroid glands
Sabrina Corbetta
Endocrinology and Diabetology Unit, Department of Medical Surgical Sciences, University of Milan, IRCCS Policlinico S Donato, Via Morandi 30, S Donato M se MI, Milan, Italy
Am J Pathol 175:1200-7. 2009
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- Stem cell therapies to treat muscular dystrophy: progress to date
Mirella Meregalli
Stem Cell Laboratory, Dipartimento di Scienze Neurologiche, Centro Dino Ferrari, Universita di Milano, Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, Milan, Italy
BioDrugs 24:237-47. 2010
..We also emphasize that the most promising possibility for the management and therapy of DMD and BMD is a combination of different approaches, such as gene and stem cell therapy...
- Effect of human skin-derived stem cells on vessel architecture, tumor growth, and tumor invasion in brain tumor animal models
Federica Pisati
Stem Cell Laboratory, Department of Neurological Science, Centro Dino Ferrari, University of Milan, Milan, Italy
Cancer Res 67:3054-63. 2007
..Taken together, these data validate the use of hSDSCs for targeting human brain tumors. They may represent therapeutically effective cells for the treatment of intracranial tumors after autologous transplantation...
- Inclusion body myopathy and frontotemporal dementia caused by a novel VCP mutation
Anna Bersano
Dino Ferrari Centre, Department of Neurological Sciences, University of Milan, IRCCS Foundation Ospedale Maggiore Policlinico Mangiagalli and Regina Elena, Via F Sforza 35, 20122 Milan, Italy
Neurobiol Aging 30:752-8. 2009
..These findings suggest VCP gene investigation even in apparently sporadic cases...
- Induction of neurotrophin expression via human adult mesenchymal stem cells: implication for cell therapy in neurodegenerative diseases
Federica Pisati
Fondazione IRCCS Ospedale Maggiore, Department of Neurological Sciences, Stem Cell Laboratory, Dino Ferrari Center, University of Milan, Milan, Italy
Cell Transplant 16:41-55. 2007
..hMSCs are further defined in their transplantation potential for treating neurological disorders...
- Combining stem cells and exon skipping strategy to treat muscular dystrophy
Mirella Meregalli
Stem Cell Laboratory, Department of Neurological Sciences, University of Milan, Padiglione Ponti, Fondazione IRCCS, Ospedale Policlinico, Via Francesco Sforza 35, 20122 Milan, Italy
Expert Opin Biol Ther 8:1051-61. 2008
..Characterization of the dystrophin gene and evidence that different types of adult stem cells are capable of muscle regeneration has lead to the development of potential gene therapy and stem cell treatments for DMD...
- Mesenchymal stem cell transplantation for neurodegenerative diseases
Yvan Torrente
Stem Cell Laboratory, Department of Neurological Science, Fondazione IRCCS Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Milan, Italy
Cell Transplant 17:1103-13. 2008
..hMSCs transplantations therefore still have their "dark side." However, the challenge in well-planned clinical trials merits discussion...
- Alternative sources of neurons and glia from somatic stem cells
Yvan Torrente
IRCCS Ospedale Maggiore Policlinico, Italy
Cell Transplant 11:25-34. 2002
..Brain engraftment of the somatic-derived neural stem cells generated neuronal phenotypes, demonstrating the great plasticity of these cells with potential clinical application...
- Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblasts
Maurilio Sampaolesi
Stem Cell Research Institute, H. S. Raffaele, Via Olgettina 58, 20132 Milan, Italy
Science 301:487-92. 2003
..The success of this protocol was mainly due to widespread distribution of donor stem cells through the capillary network, a distinct advantage of this strategy over previous approaches...
- Galectin-1 induces skeletal muscle differentiation in human fetal mesenchymal stem cells and increases muscle regeneration
Jerry Chan
Institute of Reproductive and Developmental Biology, Imperial College London, Hammersmith Campus, London, United Kingdom
Stem Cells 24:1879-91. 2006
..The high degree of myogenic conversion achieved by this method has relevance for the development of therapies for muscular dystrophies...
- Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
Beatriz G Galvez
Stem Cell Research Institute, San Raffaele Hospital, 20132 Milan, and Department of Experimental Medicine, Human Anatomy Institute, University of Pavia, Italy
J Cell Biol 174:231-43. 2006
..This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies...
- Stem and progenitor cells in skeletal muscle development, maintenance, and therapy
Bruno Peault
Stem Cell Research Center, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Mol Ther 15:867-77. 2007
..As an alternative to myoblast transplantation, stem cells such as mesoangioblasts and CD133+ progenitors administered through blood circulation have recently shown great potential to regenerate dystrophic muscle...
- Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells
Arianna Dellavalle
Stem Cell Research Institute, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy
Nat Cell Biol 9:255-67. 2007
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