Bas van Steensel

..Our data demonstrate that chromosomes fold into areas of active chromatin and areas of inactive chromatin and establish 4C technology as a powerful tool to study nuclear architecture...

..Taken together, these results demonstrate that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus...

..This review discusses the principles that drive the spatial architecture of chromatin, as well as genome-wide-binding patterns of chromatin proteins...

..Data sets obtained with these techniques begin to offer the first comprehensive views of genetic and epigenetic regulatory networks...

..Our network model provides a global view of the targeting interplay among dozens of chromatin components...

..Our approach for revealing determinants of context-dependent DNA binding will be applicable to many other transcription factors...

..These results demonstrate that HP1-chromatin is transcriptionally active and has extensive links with several other chromatin components...

..Colocalization hotspots constitute a previously uncharacterized type of feature in the genome of Drosophila, and our results provide insights into the general targeting mechanisms of transcription regulators in a higher eukaryote...

..Our results demonstrate that many interactions among PcG target genes exist and that these interactions are guided by overall chromosome architecture...

..Our results provide insights into the evolution of LAD organization and identify SU(HW) as a fine-tuner of genome - NL interactions...

..Finally, we provide evidence that the different chromatin types help to target DNA-binding factors to specific genomic regions. These results provide a global view of chromatin diversity and domain organization in a metazoan cell...

..These results indicate that HP1 acts as a docking platform for several mediator proteins that contribute to heterochromatin function...

..These results uncover a surprising link between heterochromatin and a large family of regulatory genes in mammals. We suggest a role for heterochromatin in the evolution of the KRAB-ZNF gene family...

..These results demonstrate that, depending on chromosomal location, the HP1 and Su(var)3-9 proteins form different complexes that associate with specific sets of developmentally coexpressed genes...

..Our results indicate that a substantial portion of the Drosophila genome is packaged into functionally coherent, multi-gene chromatin domains. This has broad mechanistic implications for gene regulation and genome evolution...

..These results provide insights into the mechanisms of HP1 targeting in the natural genomic context...

..These changes are independent of transcriptional changes. Our results show that the H3.3 protein counteracts association of H1, providing a mechanism to keep diverse genomic sites in an open chromatin conformation...

..These results suggest that lamina-genome interactions are widely involved in the control of gene expression programs during lineage commitment and terminal differentiation...

..This genome-wide analysis gives clear insight into the nature and dynamic behavior of the genome at the nuclear lamina, and implies that intergenic DNA functions in the global organization of chromatin in the nucleus...

..These results highlight the extensive involvement of PcG proteins in the coordination of development through the formation of large repressive chromatin domains...

..These genome-wide datasets uncover new architectural principles of eukaryotic genomes and indicate that multigene chromatin domains are prevalent and important regulatory units...

..Depending on the strategy used for expression of the Dam-fusion proteins, genome-wide binding maps can be obtained in as little as 2 weeks...

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..This chapter discusses DamID technology in detail, and a step-by-step experimental protocol is provided for use in Drosophila cell lines...

..Genome-wide mapping approaches provide new opportunities to decipher this code, but they may suffer from systematic biases. Integration of datasets and improved technologies will provide the way forward...

..New studies, including that of Gullerova and Proudfoot (2008) in this issue, reveal how cohesin is targeted to specific sites on chromosomes and implicate cohesin in the regulation of gene expression...

..Experimental tools are now available to begin to unravel the underlying molecular mechanisms...

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..These results suggest that a fundamental aspect of Max network function involves widespread binding and regulation of gene expression...

..As this correlation does not exist in S. cerevisiae, this interplay between DNA replication and transcription may be a unique characteristic of higher eukaryotes...

..Within the targeted genes, the DCC exhibits a strong preference for exons and coding sequences. Our results demonstrate gene-specific binding of the DCC, and identify several sequence elements that may partly direct its targeting...

..Finally, SUUR target genes are repressed in Drosophila embryos and gradually activated later in development. Together these results suggest that SUUR is a ubiquitous marker of heterochromatin in different cell types...

..These results define a blueprint for the transition from stem cell self-renewal to terminal differentiation...

..Methylation profiling has potential applications in disease research and diagnostic screening...