Research Topics
| Marcel TijstermanSummaryAffiliation: Netherlands Institute of Ecology Country: The Netherlands Publications
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Detail Information
Publications
The genetics of RNA silencingMarcel Tijsterman
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Annu Rev Genet 36:489-519. 2002..This chapter gives an overview of both biochemical and genetic approaches leading to the current understanding of the molecular mechanism of RNA silencing and its probable biological function...- Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAiJoris Pothof
Hubrecht Laboratory, Center for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
Genes Dev 17:443-8. 2003..Because known mutator genes are causally involved in many hereditary and sporadic human cancers, it is likely that some of these new mutators are equally relevant in cancer etiology... - PPW-1, a PAZ/PIWI protein required for efficient germline RNAi, is defective in a natural isolate of C. elegansMarcel Tijsterman
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Curr Biol 12:1535-40. 2002..Our data indicate that at least two members of this family are required for complete and effective RNAi in C. elegans... - Gene expression: long-term gene silencing by RNAiNadine L Vastenhouw
Hubrecht Laboratory-KNAW, University of Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Nature 442:882. 2006..Our findings may prove useful in the ongoing development of RNAi to treat disease... - Genes required for systemic RNA interference in Caenorhabditis elegansMarcel Tijsterman
Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Curr Biol 14:111-6. 2004..Analysis of these mutants suggests that dsRNA uptake is a specific process involving several unique proteins... - Identification of conserved pathways of DNA-damage response and radiation protection by genome-wide RNAiGijs van Haaften
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Curr Biol 16:1344-50. 2006.... - A robust network of double-strand break repair pathways governs genome integrity during C. elegans developmentDaphne B Pontier
Hubrecht Institute, Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Curr Biol 19:1384-8. 2009..Our results demonstrate profound versatility in DSB repair pathways for somatic cells of C. elegans, which are thus extremely fit to deal with chromosomal breaks... - RAP-1 and the RAL-1/exocyst pathway coordinate hypodermal cell organization in Caenorhabditis elegansEster W Frische
Department of Physiological Chemistry, Centre for Biomedical Genetics, UMC Utrecht, Utrecht, The Netherlands
EMBO J 26:5083-92. 2007..Genetic interactions show that the RAP-1 and RAL-1/exocyst pathway also act in parallel during larval stages. Together these data provide in vivo evidence for the exocyst complex as a downstream RAL-1 effector in cell migration... - Mutagenic capacity of endogenous G4 DNA underlies genome instability in FANCJ-defective C. elegansEvelien Kruisselbrink
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Curr Biol 18:900-5. 2008..The existence of 376,000 potentially mutagenic G4 DNA sites in the human genome could have major implications for the etiology of hereditary FancJ and nonhereditary cancers... - Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethalityGijs van Haaften
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Proc Natl Acad Sci U S A 101:12992-6. 2004..Knockdown of these genes increased sensitivity to ionizing radiation and camptothecin and resulted in increased chromosomal nondisjunction. All genes have human orthologs that may play a role in human carcinogenesis... - Genomic instability and cancer: scanning the Caenorhabditis elegans genome for tumor suppressorsGijs van Haaften
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands
Oncogene 23:8366-75. 2004..Here, we discuss genomic approaches in the model organism Caenorhabditis elegans, aimed to identify genes and genetic networks that contribute to genome stability and are thus potentially involved in human carcinogenesis... - Isolation of deletion alleles by G4 DNA-induced mutagenesisDaphne B Pontier
Hubrecht Institute Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands
Nat Methods 6:655-7. 2009..Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes... - Dicers at RISC; the mechanism of RNAiMarcel Tijsterman
Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
Cell 117:1-3. 2004..Two papers in this issue of Cell now demonstrate that Dicer is also essential for the execution phase of RNAi and explore the distinct requirements for Dicers in the siRNA and miRNA pathways... - Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAiFemke Simmer
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Curr Biol 12:1317-9. 2002..The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens... - Frequent germline mutations and somatic repeat instability in DNA mismatch-repair-deficient Caenorhabditis elegansMarcel Tijsterman
Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
Genetics 161:651-60. 2002..elegans as a model system to discover genes involved in maintaining DNA stability by large-scale RNAi screens... - RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAsMarcel Tijsterman
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands
Science 295:694-7. 2002..Our data favor the hypothesis that gene silencing is accomplished by RNA primer extension using the mRNA as template, leading to dsRNA that is subsequently degraded...